Molecular analysis of the cryptic and functional phn operons for phosphonate use in Escherichia coli K-12.

abstract：:Containment strategies for outbreaks of invasive Neisseria meningitidis disease are informed by serogroup assays that characterize the polysaccharide capsule. We sought to uncover the genomic basis of conflicting serogroup assay results for an isolate (M16917) from a patient with acute meningococcal disease. To this e...

journal_title：Journal of bacteriology

authors： Rishishwar L,Katz LS,Sharma NV,Rowe L,Frace M,Dolan Thomas J,Harcourt BH,Mayer LW,Jordan IK

更新日期：2012-10-01 00:00:00

abstract：:In Escherichia coli, the additon of ethanol resulted in the synthesis of an increased proportion of phospholipids containing two unsaturated fatty acids. The addition of hexanol resulted in the opposite effect, an increase in the proportion of monounsaturated molecular species. The alcohol-induced changes were quantit...

journal_title：Journal of bacteriology

authors： Berger B,Carty CE,Ingram LO

更新日期：1980-06-01 00:00:00

abstract：:Aeromonas salmonicida subsp. salmonicida, the etiological agent of furunculosis, is an important fish pathogen. We have screened this bacterium with a broad-host-range probe directed against yscV, the gene that encodes the archetype of a highly conserved family of inner membrane proteins found in every known type III ...

journal_title：Journal of bacteriology

authors： Burr SE,Stuber K,Wahli T,Frey J

更新日期：2002-11-01 00:00:00

abstract：:Escherichia coli lactose permease mediates the proton-driven translocation of galactosides across the cytoplasmic membrane. To define regions important for membrane insertion as well as for biological function, we constructed plasmids encoding different portions of the lactose carrier. Among several lacY deletions, tw...

journal_title：Journal of bacteriology

authors： Wrubel W,Stochaj U,Sonnewald U,Theres C,Ehring R

更新日期：1990-09-01 00:00:00

abstract：:H10407 is a strain of enterotoxigenic Escherichia coli (ETEC) that utilizes CFA/I pili to adhere to surfaces of the small intestine, where it elaborates toxins that cause profuse watery diarrhea in humans. Expression of the CFA/I pilus is positively regulated at the level of transcription by CfaD, a member of the AraC...

journal_title：Journal of bacteriology

authors： Pilonieta MC,Bodero MD,Munson GP

更新日期：2007-07-01 00:00:00

abstract：:Type III secretion systems (T3SSs) secrete needle components, pore-forming translocators, and the translocated effectors. In part, effector recognition by a T3SS involves their N-terminal amino acids and their 5' mRNA. To investigate whether similar molecular constraints influence translocator secretion, we scrutinize...

journal_title：Journal of bacteriology

authors： Amer AA,Åhlund MK,Bröms JE,Forsberg Å,Francis MS

更新日期：2011-12-01 00:00:00

abstract：UNLABELLED:Ileal lesions of patients with Crohn's disease are colonized by adherent-invasive Escherichia coli (AIEC), which is able to adhere to and to invade intestinal epithelial cells (IEC), to replicate within macrophages, and to form biofilms on the surface of the intestinal mucosa. Previous analyses indicated the...

journal_title：Journal of bacteriology

authors： Chassaing B,Garénaux E,Carriere J,Rolhion N,Guérardel Y,Barnich N,Bonnet R,Darfeuille-Michaud A

更新日期：2015-04-01 00:00:00

abstract：:Selection for chlorate resistance yields mol (formerly chl) mutants with defects in molybdenum cofactor synthesis. Complementation and genetic mapping analyses indicated that the Klebsiella pneumoniae mol genes are functionally homologous to those of Escherichia coli and occupy analogous genetic map positions. Hypoxan...

journal_title：Journal of bacteriology

authors： Garzón A,Li J,Flores A,Casadesus J,Stewart V

更新日期：1992-10-01 00:00:00

abstract：:During insect infection Photorhabdus luminescens emits light and expresses virulence factors, including insecticidal toxin complexes (Tcs) and an RTX-like metalloprotease (Prt). Using quantitative PCR and protein assays, we describe the expression patterns of these factors both in culture and during insect infection a...

journal_title：Journal of bacteriology

authors： Daborn PJ,Waterfield N,Blight MA,Ffrench-Constant RH

更新日期：2001-10-01 00:00:00

abstract：:Eleven conditional lethal dnaG(Ts) mutations were located by chemical cleavage of heteroduplexes formed between polymerase chain reaction-amplified DNAs from wild-type and mutant dnaG genes. This entailed end labeling one DNA strand of the heteroduplex, chemically modifying the strands with hydroxylamine or osmium tet...

journal_title：Journal of bacteriology

authors： Grompe M,Versalovic J,Koeuth T,Lupski JR

更新日期：1991-02-01 00:00:00

abstract：:The RecA proteins of the unusually strong protease-constitutive mutants recA1202 and recA1211 can use RNA in addition to single-stranded DNA (ssDNA) as a cofactor in the cleavage of the LexA repressor in vitro. In the presence of rRNA or tRNA, the effectiveness of these proteins decreased in the order RecA1202 greater...

journal_title：Journal of bacteriology

authors： Wang WB,Tessman ES,Tessman I

更新日期：1988-10-01 00:00:00

abstract：:The nucleotide sequence of tnaB of the tryptophanase operon of Escherichia coli is presented. TnaB is a tryptophan-specific permease that is homologous to Mtr, a second tryptophan-specific permease, and to TyrP, a tyrosine-specific permease. Each member of this family appears to contain 11 membrane-spanning domains. ...

journal_title：Journal of bacteriology

authors： Sarsero JP,Wookey PJ,Gollnick P,Yanofsky C,Pittard AJ

更新日期：1991-05-01 00:00:00

abstract：:An open-reading-frame fragment of a Methylobacterium sp. strain AM1 gene (moxF) encoding a portion of the methanol dehydrogenase structural protein has been used as a hybridization probe to detect similar sequences in a variety of methylotrophic bacteria. This hybridization was used to isolate clones containing putati...

journal_title：Journal of bacteriology

authors： Stephens RL,Haygood MG,Lidstrom ME

更新日期：1988-05-01 00:00:00

abstract：:Thin pili of the closely related IncI1 plasmids ColIb-P9 and R64 are required only for liquid mating and belong to the type IV family of pili. They were sedimented by ultracentrifugation from culture medium in which Escherichia coli cells harboring ColIb-P9- or R64-derived plasmids had been grown, and then the pili we...

journal_title：Journal of bacteriology

authors： Yoshida T,Furuya N,Ishikura M,Isobe T,Haino-Fukushima K,Ogawa T,Komano T

更新日期：1998-06-01 00:00:00

abstract：:Acetosyringone, a phenolic inducer of the virulence (vir) genes of Agrobacterium tumefaciens, inhibited the growth of the nopaline-type strains T37 and C58 incubated under acidic conditions. In the course of a 6-day incubation with acetosyringone, avirulent clones were produced in different proportions by strains T37 ...

journal_title：Journal of bacteriology

authors： Fortin C,Nester EW,Dion P

更新日期：1992-09-01 00:00:00

abstract：:Acid titrations of intact and butanol-treated cells of Streptococcus faecalis revealed that nearly all of the intracellular K(+) ions could diffuse into the suspending medium in association with small anions, including ribonucleic acid breakdown products, when the cell membrane was damaged. In contrast, nearly all of ...

journal_title：Journal of bacteriology

authors： Marquis RE,Porterfield N,Matsumura P

更新日期：1973-05-01 00:00:00

abstract：:The human bacterial pathogens Chlamydia spp. possess a genus-specific lipopolysaccharide as a major surface antigen, the structure of which has been determined by analytical chemistry as Kdop alpha 2-8-Kdop alpha 2-4-Kdop alpha 2-6GlcNp beta 1-6-GlcNol (Kdo, 3-deoxy-D-manno-2-octulosonic acid). Immunochemical studies ...

journal_title：Journal of bacteriology

authors： Holst O,Brade L,Kosma P,Brade H

更新日期：1991-03-01 00:00:00

abstract：:Evidence is presented that progametangia in both the plus and the minus mating types of Mucor mucedo can be induced by one substance, namely (-)-trisporic acid B. A method is described for the determination of the concentration of the sex factors (trisporone, trisporic acid B, trisporic acid C) in mated cultures of Mu...

journal_title：Journal of bacteriology

authors： Van den Ende H,Wiechmann AH,Reyngoud DJ,Hendriks T

更新日期：1970-02-01 00:00:00

abstract：UNLABELLED:The role of chromosomal toxin-antitoxin (TA) systems, which are ubiquitous within the genomes of free-living bacteria, is still debated. We have scanned the Vibrio cholerae N16961 genome for class 2 TA genes and identified 18 gene pair candidates. Interestingly, all but one are located in the chromosome 2 su...

journal_title：Journal of bacteriology

authors： Iqbal N,Guérout AM,Krin E,Le Roux F,Mazel D

更新日期：2015-07-01 00:00:00

abstract：:The Escherichia coli rluD gene encodes a pseudouridine synthase responsible for the pseudouridine (Ψ) modifications at positions 1911, 1915, and 1917 in helix 69 of 23S rRNA. It has been reported that deletion of rluD in K-12 strains of E. coli is associated with extremely slow growth, increased readthrough of stop co...

journal_title：Journal of bacteriology

authors： O'Connor M,Gregory ST

更新日期：2011-01-01 00:00:00

abstract：:Eklund, Curtis (The University of Texas, Austin) and Orville Wyss. Enzyme associated with bacteriophage infection. J. Bacteriol. 84:1209-1215. 1962.-A capsule-digesting enzyme was formed when azotobacter cells were infected with bacteriophage. The enzyme appeared in the medium when the phages lysed the cells. By disru...

journal_title：Journal of bacteriology

authors： Eklund C,Wyss O

更新日期：1962-12-01 00:00:00

abstract：:The specificity of the growth requirement of Methanobacterium ruminantium strain M1 for a new coenzyme, 2-mercaptoethanesulfonic acid (HS--CoM), was examined. A variety of derivatives, analogs, and potential biosynthetic precursors of coenzyme M were tested; only a restricted range of thioether, thioester, and thiocar...

journal_title：Journal of bacteriology

authors： Balch WE,Wolfe RS

更新日期：1979-01-01 00:00:00

abstract：:An NADPH-specific disulfide reductase that is active with bis-gamma-glutamylcystine has been purified 1,900-fold from Halobacterium halobium to yield a homogeneous preparation of the enzyme. Purification of this novel reductase, designated bis-gamma-glutamylcystine reductase (GCR), and purification of halobacterial di...

journal_title：Journal of bacteriology

authors： Sundquist AR,Fahey RC

更新日期：1988-08-01 00:00:00

abstract：:In bacteria, normal mutation frequencies are mostly around 10(-10) per base pair. However, there exists natural isolates, called "mutators," that exhibit permanent mutation occurrences up to 1,000-fold greater than usual. As mutations play essential roles, particularly in the evolution of antibiotic resistance, bacter...

journal_title：Journal of bacteriology

authors： Le Bars H,Bousarghin L,Bonnaure-Mallet M,Jolivet-Gougeon A,Barloy-Hubler F

更新日期：2012-07-01 00:00:00

abstract：:The pink yeast Rhodotorula rubra of marine origin was found to be capable of extended growth at very low phosphate concentrations (K(0.5) = 10.8 nm). Average intracellular phosphate concentrations, based on isotope exchange techniques, were 15 to 200 nm, giving concentration gradients across the cell envelope of about...

journal_title：Journal of bacteriology

authors： Button DK,Dunker SS,Morse ML

更新日期：1973-02-01 00:00:00

abstract：:Mutagenesis of Nitrosomonas europaea was achieved by electroporation and recombination. To demonstrate this, an aminoglycoside 3'-phosphotransferase (kan) gene was specifically inserted into each of the three gene copies of hao individually. Southern hybridizations and PCR analysis showed the incorporation of the kan ...

journal_title：Journal of bacteriology

authors： Hommes NG,Sayavedra-Soto LA,Arp DJ

更新日期：1996-07-01 00:00:00

abstract：:The nitrogen assimilation control protein (NAC) binds to a site within the promoter region of the histidine utilization operon (hutUH) of Klebsiella aerogenes, and NAC bound at this site activates transcription of hutUH. This NAC-binding site was characterized by a combination of random and directed DNA mutagenesis. M...

journal_title：Journal of bacteriology

authors： Pomposiello PJ,Janes BK,Bender RA

更新日期：1998-02-01 00:00:00

abstract：:Polyphosphate of average chain length corresponding to 10 phosphate units was detected in the mycelial extract of zygomycetes. Gel electrophoresis techniques commonly used for the separation and characterization of acidic mucopolysaccharides were successfully used for the detection, purification, and characterization ...

journal_title：Journal of bacteriology

authors： Dietrich SM

更新日期：1976-09-01 00:00:00

abstract：:In the filamentous cyanobacterium Anabaena variabilis, specialized cells called heterocysts occur in a regular pattern along the filament and are the sites of nitrogen fixation. We used two different types of DNA-excess RNA hybridization techniques to estimate the number of genes expressed in recently differentiated, ...

journal_title：Journal of bacteriology

authors： Lynn ME,Bantle JA,Ownby JD

更新日期：1986-09-01 00:00:00

abstract：:To investigate the function of spot 42 RNA, a small RNA of Escherichia coli, we constructed a strain in which spf, the structural gene for this RNA, is deleted. We achieved this by using a delta att phage lambda carrying a DNA fragment spanning the spf region but with a precise deletion of spf. By integration of this ...

journal_title：Journal of bacteriology

authors： Hatfull GF,Joyce CM

更新日期：1986-06-01 00:00:00