Specificity and biological distribution of coenzyme M (2-mercaptoethanesulfonic acid).

Abstract:

:The specificity of the growth requirement of Methanobacterium ruminantium strain M1 for a new coenzyme, 2-mercaptoethanesulfonic acid (HS--CoM), was examined. A variety of derivatives, analogs, and potential biosynthetic precursors of coenzyme M were tested; only a restricted range of thioether, thioester, and thiocarbonate derivatives of the cofactor were found to replace the HS--CoM requirement. Bromoethanesulfonic acid (BrCH2CH2SO3-), a halogenated analog of HS--CoM, potently inhibited the growth response. No coenzyme was detectable in a wide range of nonmethanogenic eucaryotic tissues and procaryotic organisms. However, all methanogens available in pure culture exhibited high levels of coenzyme M which ranged from 0.3 to 16 nmol/mg of dry weight.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Balch WE,Wolfe RS

doi

10.1128/JB.137.1.256-263.1979

keywords:

subject

Has Abstract

pub_date

1979-01-01 00:00:00

pages

256-63

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

137

pub_type

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