New aerobactin-mediated iron uptake system in a septicemia-causing strain of Enterobacter cloacae.

Abstract:

:Unlike the great majority of the aerobactin-producing enteric bacteria documented in the literature, Enterobacter cloacae EK33, isolated from a case of human neonatal meningitis, did not show any homology at the DNA level with the prototype aerobactin system encoded by the ColV-K30 plasmid. However, both the nuclear magnetic resonance spectrum and fast-atom bombardment mass spectrometry of the siderophore purified from EK33 confirmed its identity with aerobactin. Bioassay screening of a gene library of total DNA of EK33 led to the isolation of several aerobactin-positive clones. Under conditions of iron limitation, these clones expressed in Escherichia coli a protein of 72 kilodaltons that reacted with antiserum raised against the pColV-K30 74-kilodalton aerobactin receptor, while the original E. cloacae strain synthesized an 85-kilodalton protein which also cross-reacted with the antiserum. Restriction endonuclease analysis of the cloned DNA confirmed the structural differences between the two aerobactin genetic systems.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Crosa LM,Wolf MK,Actis LA,Sanders-Loehr J,Crosa JH

doi

10.1128/jb.170.12.5539-5544.1988

subject

Has Abstract

pub_date

1988-12-01 00:00:00

pages

5539-44

issue

12

eissn

0021-9193

issn

1098-5530

journal_volume

170

pub_type

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