Abstract:
:Mevinolinic acid, the open acid form of mevinolin, which is a metabolite of Aspergillus terreus, has been shown to be a competitive inhibitor of the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (Alberts et al., Proc. Natl. Acad. Sci. U.S.A. 77:3957-3961, 1980). The biosynthesis of mevinolinic acid was studied by examining the incorporation of [1-14C]acetate and [methyl-14C]methionine into the molecule. These isotopes were rapidly incorporated into mevinolinic acid, with [1-14C]acetate and [methyl-14C]methionine incorporation being linear for at least 10 and 30 min, respectively. A comparison of acetate incorporation into mevinolinic acid and fatty acids indicated that mevinolinic acid biosynthesis increased with a maximum between days 3 and 5 of growth; at this time cell growth had ceased and fatty acid biosynthesis was negligible. Hydrolysis of the mevinolinic acid and isolation of the products showed that [1-14C]acetate and [methyl-14C]methionine were incorporated into the 2-methylbutyric acid side chain as well as into the main (alcohol) portion of the molecule.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Greenspan MD,Yudkovitz JBdoi
10.1128/JB.162.2.704-707.1985subject
Has Abstractpub_date
1985-05-01 00:00:00pages
704-7issue
2eissn
0021-9193issn
1098-5530journal_volume
162pub_type
杂志文章abstract::A thermosensitive sporulation mutant of Bacillus subtilis containing a mutation in the secY gene was isolated and characterized. No asymmetric septum specific to the sporulation was detected by electron microscopy at the nonpermissive temperature, indicating that the block occurred at a very early stage of sporulation...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.11.3656-3660.1993
更新日期:1993-06-01 00:00:00
abstract::Granular structures containing inorganic polyphosphate were found in Micrococcus lysodeikticus. These structures were isolated by fractionation of the bacterial extract obtained by lysing the organisms with lysozyme. The composition of the fraction which was enriched with these structures was found to be: protein, 24%...
journal_title:Journal of bacteriology
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doi:10.1128/JB.96.2.544-553.1968
更新日期:1968-08-01 00:00:00
abstract::The brucellae are the etiological agents of brucellosis, a worldwide-distributed zoonosis. These bacteria are facultative intracellular parasites and thus are able to adjust their metabolism to the extra- and intracellular environments encountered during an infectious cycle. However, this aspect of Brucella biology is...
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journal_title:Journal of bacteriology
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更新日期:2012-12-01 00:00:00
abstract::A series of mutations causing alterations in ribosomal protein S21 was mapped close to dnaG, around 66.5 min on the Escherichia coli chromosomal linkage map. They cotransduced about 95% with this marker. These mutations define gene rpsU, which is very likely the structural gene for protein S21. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.2.603-607.1980
更新日期:1980-11-01 00:00:00
abstract::Sinorhizobium meliloti is a free-living soil bacterium which is capable of establishing a symbiotic relationship with the alfalfa plant (Medicago sativa). This symbiosis involves a network of bacterium-host signaling, as well as the potential for bacterium-bacterium communication, such as quorum sensing. In this study...
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更新日期:2002-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.3549-3552.1989
更新日期:1989-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.11.3587-3590.1991
更新日期:1991-06-01 00:00:00
abstract::Ellar, D. J. (Syracuse University, Syracuse, N.Y.), and D. G. Lundgren. Fine structure of sporulation in Bacillus cereus grown in a chemically defined medium. J. Bacteriol. 92:1748-1764. 1966.-A study was made of the fine structure of sporulating cells of Bacillus cereus grown in a chemically defined medium. The devel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.6.1748-1764.1966
更新日期:1966-12-01 00:00:00
abstract::Pseudomonas aeruginosa hemolytic phospholipase C (PlcH) degrades phosphatidylcholine (PC), an abundant lipid in cell membranes and lung surfactant. A ΔplcHR mutant, known to be defective in virulence in animal models, was less able to colonize epithelial cell monolayers and was defective in biofilm formation on plasti...
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pub_type: 杂志文章
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更新日期:2013-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.1.262-265.1966
更新日期:1966-01-01 00:00:00
abstract::Proteus mirabilis, a leading cause of catheter-associated urinary tract infection (CaUTI), differentiates into swarm cells that migrate across catheter surfaces and medium solidified with 1.5% agar. While many genes and nutrient requirements involved in the swarming process have been identified, few studies have addre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2013-03-01 00:00:00
abstract::A series of studies were performed on a Staphylococcus aureus strain thought to contain a pencillinase plasmid integrated into the host chromosome. Reassociation kinetics analysis of whole-cell deoxyribonucleic acid (DNA) in the presence of pure radioactive plasmid DNA revealed that plasmid-specific sequences were pre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1424-1428.1975
更新日期:1975-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.3.1476-1486.1977
更新日期:1977-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00071-13
更新日期:2013-09-01 00:00:00
abstract::Rhodospirillum rubrum was shown to grow fermentatively on fructose with N2 as a nitrogen source. The nitrogenase activity of these cells was regulated by the NH4+ switch-off/switch-on mechanism in a manner identical to that for photosynthetically grown cells. In vitro, the inactive nitrogenase Fe protein from fermenti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.3.1322-1324.1985
更新日期:1985-06-01 00:00:00
abstract::Expression of the lower catabolic pathway of the TOL plasmid pWWO requires an aromatic acid inducer and the product of the xylS regulatory gene. Pseudomonas putida cells transformed with a plasmid containing the operator-promoter region of the lower pathway (OP2 [or Pm]), upstream from the catechol 2,3-dioxygenase str...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.8.3742-3746.1988
更新日期:1988-08-01 00:00:00
abstract::Expression levels of Bacillus amyloliquefaciens alpha-amylase, Escherichia coli TEM-beta-lactamase, and Semliki Forest virus glycoprotein E1 genes were compared in Bacillus subtilis. All three model genes were expressed by using a secretion vector, constructed by joining the B. amyloliquefaciens alpha-amylase promoter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.1.176-182.1985
更新日期:1985-04-01 00:00:00
abstract::Phosphotransacetylase and acetate kinase catalyze the activation of acetate to acetyl coenzyme A in the first step of methanogenesis from acetate in Methanosarcina thermophila. The genes encoding these enzymes (pta and ack) have been cloned and sequenced. They are arranged on the chromosome with pta upstream of ack (M...
journal_title:Journal of bacteriology
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doi:10.1128/jb.177.7.1699-1702.1995
更新日期:1995-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.2.811-819.1972
更新日期:1972-02-01 00:00:00
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更新日期:2002-06-01 00:00:00
abstract::Thiamine pyrophosphate (TPP), the biologically active form of thiamine (also known as vitamin B1), is an essential cofactor for several important enzymes involved in carbohydrate metabolism, and therefore, it is required for all living organisms. We recently found that a thiamine-binding protein (TDE_0143) is essentia...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02472-14
更新日期:2015-04-01 00:00:00
abstract::The hpaB gene encoding an aromatic hydroxylase of Escherichia coli ATCC 11105, a penicillin G acylase-producing strain, has been cloned and expressed in E. coli K-12. This gene was located near the pacA gene coding for penicillin G acylase. The hydroxylase has a molecular mass of 59,000 Da, uses NADH as a cosubstrate,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1993-04-01 00:00:00
abstract:UNLABELLED:Ileal lesions of patients with Crohn's disease are colonized by adherent-invasive Escherichia coli (AIEC), which is able to adhere to and to invade intestinal epithelial cells (IEC), to replicate within macrophages, and to form biofilms on the surface of the intestinal mucosa. Previous analyses indicated the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02499-14
更新日期:2015-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1658-1665.1988
更新日期:1988-04-01 00:00:00
abstract::Green fluorescent protein (GFP) fusions, immunofluorescence microscopy, and cryo-electron tomography revealed that the chemoreceptors of the Lyme disease spirochete Borrelia burgdorferi form long, thin arrays near both cell poles. These arrays are in close proximity to the flagellar motors. This information provides a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2011-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.1.346-349.1986
更新日期:1986-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00157-07
更新日期:2007-07-01 00:00:00
abstract::An Escherichia coli F19 recA, nitrate reductase-deficient mutant was constructed by transposon mutagenesis and shown to be resistant to metronidazole. This mutant was a most suitable host for the isolation of Clostridium acetobutylicum genes on recombinant plasmids, which activated metronidazole and rendered the E. co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.3.1088-1095.1991
更新日期:1991-02-01 00:00:00
abstract::The EcfG-type sigma factor RpoE2 is the regulator of the general stress response in Sinorhizobium meliloti. RpoE2 activity is negatively regulated by two NepR-type anti-sigma factors (RsiA1/A2), themselves under the control of two anti-anti-sigma factors (RsiB1/B2) belonging to the PhyR family of response regulators. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01623-14
更新日期:2014-07-01 00:00:00