Physical aggregation and functional reconstitution of solubilized membranes of Bacillus stearothermophilus.

abstract：:Bacillus subtilis DegS-DegU belongs to a bacterial two-component system that controls many processes, including the production of exocellular proteases and competence development. It was found that when the glutamine synthetase gene glnA, which is involved in nitrogen regulation, was disrupted, the expression of the r...

journal_title：Journal of bacteriology

authors： Yasumura A,Abe S,Tanaka T

更新日期：2008-08-01 00:00:00

abstract：:Two temperature-sensitive sporulation mutants have been characterized. One mutant, which is blocked at stage II, has a short temperature-sensitive period that occurs at about the time when the spore septum is formed. Cells can escape the sporulation block, if incubated for a short period at the permissive temperature,...

journal_title：Journal of bacteriology

authors： Young M

更新日期：1976-05-01 00:00:00

abstract：:In wild-type Escherichia coli, fumarate reductase encoded by the frd operon is inducible by its substrate in the absence of molecular oxygen and nitrate. Synthesis of this enzyme under permissive conditions requires the fnr+ gene product, which is believed to be a pleiotropic regulatory protein that activates transcri...

journal_title：Journal of bacteriology

authors： Iuchi S,Kuritzkes DR,Lin EC

更新日期：1985-03-01 00:00:00

abstract：:Marchette, Nyven J. (University of Utah, Salt Lake City), and Paul S. Nicholes. Virulence and citrulline ureidase activity of Pasteurella tularensis. J. Bacteriol. 82:26-32. 1961.-The presence of a citrulline ureidase system in Pasteurella tularensis strains of high virulence, and its absence in avirulent strains and ...

journal_title：Journal of bacteriology

authors： MARCHETTE NJ,NICHOLES PS

更新日期：1961-07-01 00:00:00

abstract：:The Bacillus subtilis dciA operon encodes a dipeptide transport complex that is induced rapidly as cells enter stationary phase and initiate sporulation. Expression of this operon in growing cells is repressed by glucose, by a mixture of amino acids, and by the AbrB protein. A genetic screen was devised to identify mu...

journal_title：Journal of bacteriology

authors： Slack FJ,Mueller JP,Sonenshein AL

更新日期：1993-08-01 00:00:00

abstract：:The discovery of a novel multicistronic operon that encodes phosphofructokinase, pyruvate kinase, and lactate dehydrogenase in the lactic acid bacterium Lactococcus lactis is reported. The three genes in the operon, designated pfk, pyk, and ldh, contain 340, 502, and 325 codons, respectively. The intergenic distances ...

journal_title：Journal of bacteriology

authors： Llanos RM,Harris CJ,Hillier AJ,Davidson BE

更新日期：1993-05-01 00:00:00

abstract：:Lysozyme is an important component of the innate immune system. It functions by hydrolyzing the peptidoglycan (PG) layer of bacteria. The human pathogen Listeria monocytogenes is intrinsically lysozyme resistant. The peptidoglycan N-deacetylase PgdA and O-acetyltransferase OatA are two known factors contributing to it...

journal_title：Journal of bacteriology

authors： Rismondo J,Schulz LM,Yacoub M,Wadhawan A,Hoppert M,Dionne MS,Gründling A

更新日期：2021-01-25 00:00:00

abstract：:DNA helicase II, the product of the uvrD gene, has been implicated in DNA repair, replication, and recombination. Because the phenotypes of individual uvrD alleles vary significantly, we constructed deletion-insertion mutations in the uvrD gene to determine the phenotype of cells lacking DNA helicase II. Deletion muta...

journal_title：Journal of bacteriology

authors： Washburn BK,Kushner SR

更新日期：1991-04-01 00:00:00

abstract：:Methods were devised to purify the cell-associated, iron-binding compounds known as mycobactins from the closely related species Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum (i.e., the MAIS complex of organisms). The mycobactins from these three species showed a structure that is c...

journal_title：Journal of bacteriology

authors： Barclay R,Ewing DF,Ratledge C

更新日期：1985-11-01 00:00:00

abstract：:Streptomyces sp. strain HK803 produces six analogues of phoslactomycin (Plm A through Plm F). With the exception of Plm B, these analogues contain a C-18 hydroxyl substituent esterified with a range of short-alkyl-chain carboxylic acids. Deletion of the plmS(2) open reading frame (ORF), showing high sequence similarit...

journal_title：Journal of bacteriology

authors： Ghatge MS,Reynolds KA

更新日期：2005-12-01 00:00:00

abstract：:An 8,883-bp mini-pXO1 plasmid containing a replicon from Bacillus anthracis pXO1 (181.6 kb) was identified by making large deletions in the original plasmid using a newly developed Cre-loxP system. Portions of the truncated mini-pXO1 were cloned into an Escherichia coli vector unable to replicate in B. anthracis. A 5....

journal_title：Journal of bacteriology

authors： Pomerantsev AP,Camp A,Leppla SH

更新日期：2009-08-01 00:00:00

abstract：:Pore-forming colicins are soluble bacteriocins which form voltage-gated ion channels in the inner membrane of Escherichia coli. To reach their target, these colicins first bind to a receptor located on the outer membrane and then are translocated through the envelope. Colicins are subdivided into two groups according ...

journal_title：Journal of bacteriology

authors： Duché D,Letellier L,Géli V,Bénédetti H,Baty D

更新日期：1995-09-01 00:00:00

abstract：:The nucleotide sequence of the DNA encoding the ADP-ribosyltransferase (A1) fragment of cholera enterotoxin was determined. A putative precursor of the A1 peptide contains an 18-amino acid leader peptide, and the mature A1 peptide contains 194 amino acids. The primary structure of the A1 fragment from cholera enteroto...

journal_title：Journal of bacteriology

authors： Lockman HA,Galen JE,Kaper JB

更新日期：1984-09-01 00:00:00

abstract：:A cosmid library of copper-resistant (Cur) Pseudomonas syringae pv. tomato PT23 plasmid DNA was constructed and mobilized into the copper-sensitive recipient P. syringae pv. syringae PS61. One resultant cosmid clone, pCOP1 (46 kilobases), conferred copper resistance. The PT23 Cur gene(s) was located on pCOP1 by subclo...

journal_title：Journal of bacteriology

authors： Bender CL,Cooksey DA

更新日期：1987-02-01 00:00:00

abstract：:In the bacterium Escherichia coli, oxidized pyrimidines are removed by two DNA glycosylases, endonuclease III and endonuclease VIII (endo VIII), encoded by the nth and nei genes, respectively. Double mutants lacking both of these activities exhibit a high spontaneous mutation frequency, and here we show that all of th...

journal_title：Journal of bacteriology

authors： Blaisdell JO,Hatahet Z,Wallace SS

更新日期：1999-10-01 00:00:00

abstract：:Rhodococcus equi is a facultative intracellular pathogen which proliferates rapidly in both manure-enriched soil and alveolar macrophages. Although both environments are characterized by extremely low concentrations of free iron, very little is known regarding the strategies employed by R. equi to thrive under these c...

journal_title：Journal of bacteriology

authors： Miranda-Casoluengo R,Duffy PS,O'Connell EP,Graham BJ,Mangan MW,Prescott JF,Meijer WG

更新日期：2005-05-01 00:00:00

abstract：:An analysis of the turnover of the major phospholipids of Micrococcus cerificans growing or nongrowing cultures. The turnover rates of (14)C-PE and (14)C-PE were 61.5% of the total phospholipid, exhibited no significant rate of turnover in either growing or nongrowing cultures. The turnover rates of PE-(14)C and PE-(3...

journal_title：Journal of bacteriology

authors： Makula RA,Finnerty WR

更新日期：1971-09-01 00:00:00

abstract：:Flagellar biogenesis and hence motility of Vibrio fischeri depends upon the presence of magnesium. In the absence of magnesium, cells contain few or no flagella and are poorly motile or nonmotile. To dissect the mechanism by which this regulation occurs, we screened transposon insertion mutants for those that could mi...

journal_title：Journal of bacteriology

authors： O'Shea TM,Klein AH,Geszvain K,Wolfe AJ,Visick KL

更新日期：2006-12-01 00:00:00

abstract：:Some lines of the widely used strain CR34 of Escherichia coli K-12 carry a plasmid. The plasmid has a mass of 12 +/- 2 X 10(6) megadaltons and is maintained at a low copy number per cell (1 to 2), and this number is not amplified by growth of cells in the presence of chloramphenicol. The plasmid is designated as pCU3....

journal_title：Journal of bacteriology

authors： Wang PY,Relf J,Palchaudhuri S,Iyer VN

更新日期：1978-02-01 00:00:00

abstract：:The molecular mechanisms of regulation of the genes involved in the biosynthesis of cysteine are poorly characterized in Bacillus subtilis and other gram-positive bacteria. In this study we describe the expression pattern of the B. subtilis cysH operon in response to sulfur starvation. A 6.1-kb polycistronic transcrip...

journal_title：Journal of bacteriology

authors： Mansilla MC,Albanesi D,de Mendoza D

更新日期：2000-10-01 00:00:00

abstract：:The raffinose (raf) operon is negatively controlled by the specific binding of raf repressor (rafR gene) to raf operator (rafO) DNA. Both rafR and rafO have been sequenced. The 1,011-base-pair rafR gene encodes a 336-amino-acid polypeptide containing an N-terminal helix-turn-helix motif. rafO, as defined by in vivo ti...

journal_title：Journal of bacteriology

authors： Aslanidis C,Schmitt R

更新日期：1990-04-01 00:00:00

abstract：:Bacteria in biofilms often undergo active dispersal events and revert to a free-swimming, planktonic state to complete the biofilm life cycle. The signaling molecule nitric oxide (NO) was previously found to trigger biofilm dispersal in the opportunistic pathogen Pseudomonas aeruginosa at low, nontoxic concentrations ...

journal_title：Journal of bacteriology

authors： Barraud N,Schleheck D,Klebensberger J,Webb JS,Hassett DJ,Rice SA,Kjelleberg S

更新日期：2009-12-01 00:00:00

abstract：:Escherichia coli mutants lacking activities of all known cytosolic ATP-dependent proteases (Lon, ClpAP, ClpXP, and HslVU), due to double deletions [DeltahslVU and Delta(clpPX-lon)], cannot grow at low (30 degrees C) or very high (45 degrees C) temperatures, unlike those carrying either of the deletions. Such growth de...

journal_title：Journal of bacteriology

authors： Kanemori M,Yanagi H,Yura T

更新日期：1999-06-01 00:00:00

abstract：:A procedure is described for the purification of the Escherichia coli outer membrane (lipopolysaccharide or L membrane) with flagella still attached. The resulting lipopolysaccharide membrane was in the form of vesicles that had a trilaminar structure in thin section and contained about 55% lipopolysaccharide and 45% ...

journal_title：Journal of bacteriology

authors： DePamphilis ML,Adler J

更新日期：1971-01-01 00:00:00

abstract：:Unique lipids found in Azotobacter vinelandii cysts are derived primarily from beta-hydroxybutyrate used to induce encystment. Tracer studies with beta-[14C]hydroxybutyrate showed that the biosynthesis of these compounds during encystment began at 8 to 12 h after induction and reached maximal levels after 2 days, Seve...

journal_title：Journal of bacteriology

authors： Su CJ,Sadoff HL

更新日期：1981-07-01 00:00:00

abstract：:In this study, we demonstrated that the surface-expressed enolase from diarrheal isolate SSU of Aeromonas hydrophila bound to human plasminogen and facilitated the latter's tissue-type plasminogen activator-mediated activation to plasmin. The bacterial surface-bound plasmin was more resistant to the action of its spec...

journal_title：Journal of bacteriology

authors： Sha J,Erova TE,Alyea RA,Wang S,Olano JP,Pancholi V,Chopra AK

更新日期：2009-05-01 00:00:00

abstract：:Protein export in Escherichia coli is mediated by translocase, a multisubunit membrane protein complex with SecA as the peripheral subunit and the SecY, SecE, and SecG proteins as the integral membrane domain. In the gram-positive bacterium Bacillus subtilis, SecA, SecY, and SecE have been identified through genetic a...

journal_title：Journal of bacteriology

authors： van Wely KH,Swaving J,Broekhuizen CP,Rose M,Quax WJ,Driessen AJ

更新日期：1999-03-01 00:00:00

abstract：:Clostridium perfringens type B and D isolates produce epsilon-toxin, the third most potent clostridial toxin. The epsilon-toxin gene (etx) is plasmid borne in type D isolates, but etx genetics have been poorly studied in type B isolates. This study reports the first sequencing of any etx plasmid, i.e., pCP8533etx, fro...

journal_title：Journal of bacteriology

authors： Miyamoto K,Li J,Sayeed S,Akimoto S,McClane BA

更新日期：2008-11-01 00:00:00

abstract：:Chorpenning, Frank W. (The Ohio State University, Columbus), and Matthew C. Dodd. Heterogenetic antigens of gram-positive bacteria. J. Bacteriol. 91:1440-1445. 1966.-Soluble antigens obtained by various methods from gram-positive bacteria were used to modify erythrocytes whose hemagglutinating reactions with immune ra...

journal_title：Journal of bacteriology

authors： Chorpenning FW,Dodd MC

更新日期：1966-04-01 00:00:00

abstract：:The major acid-soluble spore proteins (ASSPs) of Bacillus subtilis were detected by immunoprecipitation of radioactively labeled in vitro- and in vivo-synthesized proteins. ASSP synthesis in vivo began 2 h after the initiation of sporulation (t2) and reached its maximum rate at t7. This corresponded to the time of syn...

journal_title：Journal of bacteriology

authors： Leventhal JM,Chambliss GH

更新日期：1982-12-01 00:00:00