Abstract：
:Escherichia coli mutants lacking activities of all known cytosolic ATP-dependent proteases (Lon, ClpAP, ClpXP, and HslVU), due to double deletions [DeltahslVU and Delta(clpPX-lon)], cannot grow at low (30 degrees C) or very high (45 degrees C) temperatures, unlike those carrying either of the deletions. Such growth defects were particularly marked when the deletions were introduced into strain MG1655 or W3110. To examine the functions of HslVU and other proteases further, revertants that can grow at 30 degrees C were isolated from the multiple-protease mutant and characterized. The revertants were found to carry a suppressor affecting either ftsZ (encoding a key cell division protein) or sulA (encoding the SulA inhibitor, which binds and inhibits FtsZ). Whereas the ftsZ mutations were identical to a mutation known to produce a protein refractory to SulA inhibition, the sulA mutations affected the promoter-operator region, reducing synthesis of SulA. These results suggested that the growth defect of the parental double-deletion mutant at a low temperature was due to the accumulation of excess SulA without DNA-damaging treatment. Consistent with these results, SulA in the double-deletion mutant was much more stable than that in the Delta(clpPX-lon) mutant, suggesting that SulA can be degraded by HslVU. As expected, purified HslVU protease degraded SulA (fused to the maltose-binding protein) efficiently in an ATP-dependent manner. These results suggest that HslVU as well as Lon participates in the in vivo turnover of SulA and that HslVU becomes essential for growth when the Lon (and Clp) protease level is reduced below a critical threshold.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kanemori M,Yanagi H,Yura Tdoi
10.1128/JB.181.12.3674-3680.1999keywords:
subject
Has Abstractpub_date
1999-06-01 00:00:00pages
3674-80issue
12eissn
0021-9193issn
1098-5530journal_volume
181pub_type
杂志文章abstract：:In aquatic environments, Caulobacter spp. can be found at the boundary between liquid and air known as the neuston. I report an approach to study temporal features of Caulobacter crescentus colonization and pellicle biofilm development at the air-liquid interface and have defined the role of cell surface structures in...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.00064-19
更新日期：2019-08-22 00:00:00
abstract：:The transferred DNA (T-DNA) is transported from Agrobacterium tumefaciens to the nucleus and is stably integrated into the genome of many plant species. It has been proposed that the VirD2 protein, tightly attached to the T-DNA, pilots the T-DNA into the plant cell nucleus and that it is involved in integration. Using...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.175.3.723-731.1993
更新日期：1993-02-01 00:00:00
abstract：:The experiments presented in this paper suggest that the shift observed in sedimentation of deoxyribonucleic acid from cells of Escherichia coli subjected to amino acid starvation is related to inhibition of ribonucleic acid synthesis rather than to its release from the membrane at the termination of replication. ...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.137.2.790-794.1979
更新日期：1979-02-01 00:00:00
abstract：:Screening of derivatives of Rhizobium etli KIM5s randomly mutagenized with mTn5SSgusA30 resulted in the identification of strain KIM-G1. Its rough colony appearance, flocculation in liquid culture, and Ndv(-) Fix(-) phenotype were indicative of a lipopolysaccharide (LPS) defect. Electrophoretic analysis of cell-associ...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.181.18.5606-5614.1999
更新日期：1999-09-01 00:00:00
abstract：:SugE of Escherichia coli, first identified as a suppressor of groEL mutations but a member of the small multidrug resistance family, has not previously been shown to confer a drug resistance phenotype. We show that high-level expression of sugE leads to resistance to a subset of toxic quaternary ammonium compounds. ...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.184.9.2543-2545.2002
更新日期：2002-05-01 00:00:00
abstract：:Antipain kills Escherichia coli K-12 cells in an exponential manner beginning 1 h after its addition. Mutant strains, delta cya and crp, which are unable to synthesize cyclic adenosine 3',5'-monophosphate (cAMP) and the cAMP receptor protein, respectively, are not affected. Addition of cAMP (5 mM) to antipain-treated ...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.139.2.690-693.1979
更新日期：1979-08-01 00:00:00
abstract：:Calcium-dependent exonuclease activity was produced by sporulating cells of Bacillus subtilis 168. Nuclease activity was released into the culture medium at approximately the same time as sporulation proteases, and production of these enzymes was tied to DNA replication. Results suggest that nuclease production is a f...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.149.2.768-770.1982
更新日期：1982-02-01 00:00:00
abstract：:In bacteria, a subset of membrane proteins insert into the membrane via the Sec apparatus with the assistance of the widely conserved essential membrane protein insertase YidC. After threading into the SecYEG translocon, transmembrane segments of nascent proteins are thought to exit the translocon via a lateral gate i...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.01095-13
更新日期：2014-01-01 00:00:00
abstract：:Genome sequences from the prolific Roseobacter clade in the Alphaproteobacteria are beginning to reveal the genetic basis for the diverse lifestyles of these organisms. Here we present the genome sequences of Oceanicola granulosus HTCC2516(T) and Oceanicola batsensis HTCC2597(T), two marine Roseobacter species isolate...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.00412-10
更新日期：2010-07-01 00:00:00
abstract：:Membrane vesicles isolated from competent cultures of Bacillus subtilis 168 bound up to 20 mug of double-stranded deoxyribonucleic acid (DNA) per mg of membrane protein in the presence of ethylenediaminetetraacetate. The formation of the DNA-membrane complex was time, temperature, and pH dependent. Eighty per cent of ...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.119.3.784-794.1974
更新日期：1974-09-01 00:00:00
abstract：:Bladen, Howard A. (National Institute of Dental Health, Bethesda, Md.), and Edward G. Hampp. Ultrastructure of Treponema microdentium and Borrelia vincentii. J. Bacteriol. 87:1180-1191.-A small oral Treponema (FM) and Borrelia vincentii (N9) were harvested after 3 to 7 days of incubation and either embedded in Vestopa...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.87.5.1180-1191.1964
更新日期：1964-05-01 00:00:00
abstract：:Klein, Dolph (Rutgers, The State University, New Brunswick, N. J.), and David Pramer. Bacterial dissimilation of streptomycin. J. Bacteriol. 82:505-510. 1961.-The influence of various nutritional and environmental factors on the dissimilation of streptomycin by a pseudomonad isolated from soil was investigated, and co...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.82.4.505-510.1961
更新日期：1961-10-01 00:00:00
abstract：:Vibrio cholerae hemolysin (HlyA) is a 65-kDa water-soluble pore-forming toxin that causes lysis of eukaryotic cells by destroying selective permeability of the plasma membrane bilayer. The HlyA monomer self-assembles on the target cell surface to the more stable beta-barrel amphipathic heptamer, which inserts into the...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.00930-09
更新日期：2010-01-01 00:00:00
abstract：:Compared with the other class B multimodular penicillin- binding proteins (PBPs), the low-affinity PBP5 responsible for penicillin resistance in Enterococcus hirae R40, has an extended non-penicillin-binding module because of the presence of an approximately 110-amino-acid E-46(-)D-160 insert downstream from the membr...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.178.6.1774-1775.1996
更新日期：1996-03-01 00:00:00
abstract：:MreC and MreD, along with the actin homologue MreB, are required to maintain the shape of rod-shaped bacteria. The depletion of MreCD in rod-shaped bacteria leads to the formation of spherical cells and the accumulation of suppressor mutations. Ovococcus bacteria, such as Streptococcus pneumoniae, lack MreB homologues...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.05245-11
更新日期：2011-08-01 00:00:00
abstract：:The lprG-Rv1410c operon is critical for the survival of Mycobacterium tuberculosis during infection, but very little is known about the functions of its proteins. LprG is a lipoprotein, and Rv1410c encodes the major facilitator superfamily small molecule transporter P55. P55 likely exports small molecules outside of t...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.01046-07
更新日期：2008-03-01 00:00:00
abstract：:The effects of high doses of radiation (1, 5, or 20 Mrad) on the toxicity, pyrogenicity, and immunogenicity of Salmonella typhimurium cells and endotoxin were studied. Toxicity decreased progressively after exposure to 1, 5, or 20 Mrad. The lethal effect of 1-Mrad exposed cells was greater than that of heat-, acetone-...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.95.6.2165-2170.1968
更新日期：1968-06-01 00:00:00
abstract：:Cryptococcus neoformans is an important pathogenic fungus that has been classified as a basidiomycete. Little is known of the molecular genetics of this fungal pathogen. To begin such studies, we devised a procedure for extraction of DNA from cryptococci; this method involved the use of the cell wall-active enzyme Nov...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.171.10.5596-5600.1989
更新日期：1989-10-01 00:00:00
abstract：:We have identified three transposable gene-activating elements from Pseudomonas cepacia on the basis of their abilities to increase expression of the lac genes of the broad-host-range plasmid pGC91.14 (pRP1::Tn951). When introduced into auxotrophic derivatives of P. cepacia 249 (ATCC 17616), this plasmid failed to con...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.172.4.1719-1724.1990
更新日期：1990-04-01 00:00:00
abstract：:The β-barrel assembly machinery (BAM) complex is the core machinery for the assembly of β-barrel membrane proteins, and inhibition of BAM complex activity is lethal to bacteria. Discovery of integral membrane proteins that are key to pathogenesis and yet do not require assistance from the BAM complex raises the questi...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.00521-17
更新日期：2018-02-07 00:00:00
abstract：:Streptococcus pneumoniae is the causative agent of multiple diseases, including otitis media, pneumonia, bacteremia, and meningitis. Pneumolysin (Ply), a member of the cholesterol-dependent cytolytic pore-forming toxins, is produced by virtually all clinical isolates of S. pneumoniae, and strains in which the Ply gene...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.01489-08
更新日期：2009-04-01 00:00:00
abstract：:Antiserum prepared against protocatechuate 3, 4-dioxygenase from Pseudomonas aeruginosa forms precipitin bands without spurs with isofunctional enzymes from different strains of the fluorescent pseudomonads on immunodiffusion plates. Catalytic activity of the isofunctional enzymes was inhibited by an immunoglobulin fr...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.126.1.516-519.1976
更新日期：1976-04-01 00:00:00
abstract：:Wild-type Escherichia coli cells are unable to grow on beta-glucosides. Spontaneous mutants arise, however, which are able to utilize certain aromatic beta-glucosides such as salicin or arbutin as carbon sources, revealing the presence of a cryptic operon called bgl. Mutations activating the operon map within (or clos...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.169.6.2579-2590.1987
更新日期：1987-06-01 00:00:00
abstract：:An 8,883-bp mini-pXO1 plasmid containing a replicon from Bacillus anthracis pXO1 (181.6 kb) was identified by making large deletions in the original plasmid using a newly developed Cre-loxP system. Portions of the truncated mini-pXO1 were cloned into an Escherichia coli vector unable to replicate in B. anthracis. A 5....
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.00422-09
更新日期：2009-08-01 00:00:00
abstract：:The outer membranes of Escherichia coli K-12 cells carrying a derepressed F-like R factor contain about 7 times 10-4 molecules per cell of a protein similar to the subunit of the sex pili specified by the R factor. This protein pool is absent in cells carrying the repressed variant of the R factor. The size of the poo...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.122.1.59-65.1975
更新日期：1975-04-01 00:00:00
abstract：:Ribosomal subunits from Chloroflexus aurantiacus were isolated and examined by sucrose gradient sedimentation, gel electrophoresis, and electron microscopy. The 30S subunits had all the characteristic structural features of other eubacterial 30S subunits. The data support the proposal that the absence of the archaebac...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.155.2.900-902.1983
更新日期：1983-08-01 00:00:00
abstract：:Cultures of isocitrate dehydrogenase-deficient (icd) mutants were overgrown by double mutants (icd glt) lacking citrate synthase activity also. The icd mutants grew more slowly than wild-type cells or the double mutants because they accumulated an inhibitory metabolite (possibly citrate). Intracellular citrate levels ...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.127.1.76-83.1976
更新日期：1976-07-01 00:00:00
abstract：:NO, a free radical gas, is the signal for Nitrosomonas europaea cells to switch between different growth modes. At an NO concentration of more than 30 ppm, biofilm formation by N. europaea was induced. NO concentrations below 5 ppm led to a reversal of the biofilm formation, and the numbers of motile and planktonic (m...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.186.9.2781-2788.2004
更新日期：2004-05-01 00:00:00
abstract：:Variations of the membrane filter technique for deoxyribonucleic acid (DNA) hybridizations were studied with respect to Streptomyces species. At the temperatures required for specific hybridization of DNA with the high melting temperature (T(m)) characteristic of Streptomyces, large amounts (up to 97%) of filter-bound...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.104.3.1086-1094.1970
更新日期：1970-12-01 00:00:00
abstract：:In Bacillus subtilis, extracellular peptide signaling regulates several biological processes. Secreted Phr signaling peptides are imported into the cell and act intracellularly to antagonize the activity of regulators known as Rap proteins. B. subtilis encodes several Rap proteins and Phr peptides, and the processes r...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.00300-06
更新日期：2006-07-01 00:00:00
