Abstract:
:Flagellar biogenesis and hence motility of Vibrio fischeri depends upon the presence of magnesium. In the absence of magnesium, cells contain few or no flagella and are poorly motile or nonmotile. To dissect the mechanism by which this regulation occurs, we screened transposon insertion mutants for those that could migrate through soft agar medium lacking added magnesium. We identified mutants with insertions in two distinct genes, VF0989 and VFA0959, which we termed mifA and mifB, respectively, for magnesium-dependent induction of flagellation. Each gene encodes a predicted membrane-associated protein with diguanylate cyclase activity. Consistent with that activity, introduction into V. fischeri of medium-copy plasmids carrying these genes inhibited motility. Furthermore, multicopy expression of mifA induced other phenotypes known to be correlated with diguanylate cyclase activity, including cellulose biosynthesis and biofilm formation. To directly test their function, we introduced the wild-type genes on high-copy plasmids into Escherichia coli. We assayed for the production of cyclic di-GMP using two-dimensional thin-layer chromatography and found that strains carrying these plasmids produced a small but reproducible spot that migrated with an R(f) value consistent with cyclic di-GMP that was not produced by strains carrying the vector control. Disruptions of mifA or mifB increased flagellin levels, while multicopy expression decreased them. Semiquantitative reverse transcription-PCR experiments revealed no significant difference in the amount of flagellin transcripts produced in either the presence or absence of Mg(2+) by either vector control or mifA-overexpressing cells, indicating that the impact of magnesium and cyclic-di-GMP primarily acts following transcription. Finally, we present a model for the roles of magnesium and cyclic di-GMP in the control of motility of V. fischeri.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
O'Shea TM,Klein AH,Geszvain K,Wolfe AJ,Visick KLdoi
10.1128/JB.00728-06subject
Has Abstractpub_date
2006-12-01 00:00:00pages
8196-205issue
23eissn
0021-9193issn
1098-5530pii
JB.00728-06journal_volume
188pub_type
杂志文章abstract::Growth and sporulation of a Bacillus subtilis mutant deficient in branched fatty acid synthesis (gene symbol bfmB) were examined. The mutant, which produces an acyl-coenzyme A:acyl carrier protein transacylase with reduced affinity for branched fatty acid primers, could grow in media containing any one of a wide range...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.148.2.480-486.1981
更新日期:1981-11-01 00:00:00
abstract::Previous studies have implicated glutamine synthetase (L-glutamate:ammonia ligase [adenosine diphosphate for-ing], EC 6.6.1.2) as a major controlling element of the nitrogen fixation (nif) genes in Klebsiella pneumoniae. We report here the isolation of a new class of K. pneumoniae mutants which exhibit altered pattern...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.597-606.1979
更新日期:1979-11-01 00:00:00
abstract::The nucleotide sequence of a 2220-base-pair fragment containing the btuB gene of Escherichia coli was determined. There was a single open reading frame which was translated into a 614-amino-acid polypeptide, the first 20 amino acids of which comprised a typical leader sequence. The putative mature or processed form ha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.3.904-908.1985
更新日期:1985-03-01 00:00:00
abstract::Escherichia coli prefers growth in neutral pH environments but can withstand extremely acidic conditions (pH 2) for long periods. Of the four E. coli systems that contribute to acid resistance, one, the glutamate-dependent system, is remarkable in its efficacy and regulatory complexity. The resistance mechanism involv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.21.7378-7389.2004
更新日期:2004-11-01 00:00:00
abstract::Grixazone (GX), which is a diffusible yellow pigment containing a phenoxazinone chromophore, is one of the secondary metabolites under the control of A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) in Streptomyces griseus. GX production is also induced by phosphate starvation. The whole biosynthesis gen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00055-07
更新日期:2007-05-01 00:00:00
abstract::Pseudomonas aeruginosa XMG, isolated from soil, utilizes lactate. Here we present a 6.45-Mb assembly of its genome sequence. Besides the lactate utilization mechanism of the strain, the genome sequence may also provide other useful information related to P. aeruginosa, such as identifying genes involved in virulence, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00943-12
更新日期:2012-09-01 00:00:00
abstract::Here, we present the complete genome sequence of Rhodococcus pyridinivorans AK37 strain NCAIM PB1376, which was isolated from an oil-polluted site in Hungary. R. pyridinivorans AK37 is an aerobic, nonsporulating, nonmotile, gram-positive bacterium with remarkable aromatic-decomposing activity. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06603-11
更新日期:2012-03-01 00:00:00
abstract:UNLABELLED:Edwardsiella tarda is an important pathogenic bacterium that can replicate in macrophages. However, how the intramacrophage infection process affects the virulence of this bacterium is essentially unknown. Here, we show that E. tarda replicates and induces a caspase-1-dependent cell pyroptosis in a murine ma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00978-15
更新日期:2016-04-28 00:00:00
abstract::Thiobacillus denitrificans was grown anaerobically with nitrate as an acceptor in both sterile and nonsterile media. Ribulose diphosphate carboxylase was stable throughout the exponential growth phase and declined slowly only after cells reached the stationary phase. Reversible inactivation of the carboxylase occurred...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.2.633-642.1972
更新日期:1972-05-01 00:00:00
abstract::The endoplasmic reticulum from Neurospora crassa was identified by monitoring the activity of the putative enzyme marker phosphatidylcholine glyceride transferase. After differential centrifugation of a cell homogenate, phosphatidylcholine glyceride transferase activity initially copurified with plasma membrane H+-ATP...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.1.362-368.1983
更新日期:1983-10-01 00:00:00
abstract::In growing cultures of Escherichia coli, the nonacylated glycerol of phosphatidylglycerol (PG) is labeled more rapidly than is the acylated glycerol. This is, in part, due to a rapid exchange reaction of the nonacylated glycerol. Only some of the PG molecules undergo this reaction while others are stable. Using a muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.1.210-214.1973
更新日期:1973-10-01 00:00:00
abstract::Several phosphate-starvation-inducible (psi) genetic loci in Salmonella typhimurium were identified by fusing the lacZ gene to psi promoters by using the Mu d1 and Mu d1-8 bacteriophages. Although several different starvation conditions were examined, the psi loci responded solely to phosphate deprivation. A regulator...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.2.666-669.1986
更新日期:1986-05-01 00:00:00
abstract::A temperature-sensitive mutant of Escherichia coli with a temperature-dependent change in the control of initiation of DNA replication was isolated. The phenotype of the mutant was dependent on a mutation in the RNA polymerase gene rpoC. In vitro RNA polymerase activity was temperature sensitive. The mutant grew and s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.1.443-451.1983
更新日期:1983-04-01 00:00:00
abstract::The ski22::Tn917lac insertion mutation in Bacillus subtilis was isolated in a screen for mutations that cause a defect in sporulation but are suppressed by the presence or overexpression of the histidine protein kinase encoded by kinA (spoIIJ). The ski22::Tn917lac insertion mutation was in ald, the gene encoding alani...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.21.6789-6796.1993
更新日期:1993-11-01 00:00:00
abstract::We have found, using a newly developed genetic method, a protein (named Cnu, for oriC-binding nucleoid-associated) that binds to a specific 26-base-pair sequence (named cnb) in the origin of replication of Escherichia coli, oriC. Cnu is composed of 71 amino acids (8.4 kDa) and shows extensive amino acid identity to a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.20.6998-7008.2005
更新日期:2005-10-01 00:00:00
abstract::All bacteria use the conserved Sec pathway to transport proteins across the cytoplasmic membrane, with the SecA ATPase playing a central role in the process. Mycobacteria are part of a small group of bacteria that have two SecA proteins: the canonical SecA (SecA1) and a second, specialized SecA (SecA2). The SecA2-depe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00630-13
更新日期:2013-10-01 00:00:00
abstract::Bacillus subtilis mutants deficient in amino acid repression of the histidine utilization (hut) operon were isolated by transposon mutagenesis. Genetic characterization of these mutants indicated that they most likely contained transposon insertions within the codVWXY operon. The codY gene is required for nutritional ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.13.3779-3784.1996
更新日期:1996-07-01 00:00:00
abstract::The widely conserved second messenger cyclic diguanosine monophosphate (c-di-GMP) plays a key role in quorum-sensing (QS)-dependent production of virulence factors in Xanthomonas campestris pv. campestris. The detection of QS diffusible signal factor (DSF) by the sensor RpfC leads to the activation of response regulat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01253-09
更新日期:2010-02-01 00:00:00
abstract::The kil-kor regulon of the self-transmissible, broad-host-range plasmid RK2 is a unique network with eight coregulated operons. Among the genes encoded by the kil-kor regulon are trfA, which encodes the replication initiator, and several kil loci (kilA, kilB, kilC, and kilE), each of which is lethal to the host cell i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.8.2423-2435.1993
更新日期:1993-04-01 00:00:00
abstract::The photosynthetic cyanobacterium Synechocystis sp. strain PCC6803 possesses homologs of known genes of the non-mevalonate 2-C-methyl-D-erythritol 2-phosphate (MEP) pathway for synthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Isoprenoid biosynthesis in extracts of this cyanobacterium, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.18.5045-5051.2002
更新日期:2002-09-01 00:00:00
abstract::The purine regulon repressor, PurR, was identified as a component of the Escherichia coli regulatory system for pyrC, the gene that encodes dihydroorotase, an enzyme in de novo pyrimidine nucleotide synthesis. PurR binds to a pyrC control site that resembles a pur regulon operator and represses expression by twofold. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.6.3201-3207.1990
更新日期:1990-06-01 00:00:00
abstract::Uninduced cultures of Saccharomyces cerevisiae exhibit high basal levels of allantoinase, allantoicase, and ureidoglycolate hydrolase, the enzymes responsible for degrading allantoin to urea. As a result, these activities increase only 4- to 8-fold upon induction, whereas the urea-degrading enzymes, urea carboxylase a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.2.490-497.1978
更新日期:1978-08-01 00:00:00
abstract::Type 3 fimbriae are adhesive organelles found in enterobacterial pathogens. The fimbriae promote biofilm formation on biotic and abiotic surfaces; however, the exact identity of the receptor for the type 3 fimbriae adhesin, MrkD, remains elusive. We analyzed naturally occurring structural and functional variabilities ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00753-13
更新日期:2013-12-01 00:00:00
abstract::Escherichia coli O111a is a thermosensitive strain which, when grown at 40 C, accumulates large quantities of intracellular membranes. The ultrastructure of these membranes in cells which have been chemically fixed, embedded, and examined as thin sections has been compared with that of membranes in cells negatively st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.1.433-444.1973
更新日期:1973-01-01 00:00:00
abstract::RsbT is an essential component of the pathway that activates the Bacillus subtilis sigma(B) transcription factor in response to physical stress. rsbT is located within an operon that includes the genes for its principal negative regulator (RsbS) and the stress pathway component that it activates (RsbU), as immediate u...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.24.8520-8525.2005
更新日期:2005-12-01 00:00:00
abstract::Electron microscopy of thin sections of dormant and germinating spores of Bacillus subtilis 168 revealed a progressive change in the structure of the cortex, outer spore coat, and inner spore coat. The initial changes were observed in the cortex region, which showed a loose fibrous network within 10 min of germination...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.475-481.1974
更新日期:1974-10-01 00:00:00
abstract::Radiorespirometric and enzymatic analyses reveal that glucose-grown cells of Rhizobium japonicum isolates I-110 and L1-110, both derivatives of R. japonicum strain 3I1b110, possess an active tricarboxylic acid cycle and metabolize glucose by simultaneous operation of the Embden-Meyerhof-Parnas and Entner-Doudoroff pat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.1.179-187.1977
更新日期:1977-07-01 00:00:00
abstract::A thermosensitive episome bearing the transposon Tn10, F(Ts)::Tn10 Lac+, has been successfully transferred from Escherichia coli to several wild strains of the enterobacteria Erwinia carotovora subsp. chrysanthemi, which are pathogenic on Saintpaulia ionantha. In one of these strains, all of the characters controlled ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.1.122-131.1982
更新日期:1982-04-01 00:00:00
abstract::Two isoenzymes of malate dehydrogenase (MDH) were demonstrated in plasmodia of Physarum polycephalum by polyacrylamide-gel electrophoresis. The more "cathodal" form was uniquely associated with mitochondria (M-MDH) and the other form was found in the soluble cytoplasm (S-MDH). The isoenzymes were separated by acetone ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.2.673-684.1973
更新日期:1973-11-01 00:00:00
abstract::The SsuDAT1I restriction-modification (R-M) system, which contains two methyltransferases and two restriction endonucleases with recognition sequence 5'-GATC-3', was first found in a field isolate of Streptococcus suis serotype 2. Isoschizomers of the R-M system were found in the same locus between purH and purD in a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.183.18.5436-5440.2001
更新日期:2001-09-01 00:00:00