A global investigation of the Bacillus subtilis iron-sparing response identifies major changes in metabolism.

abstract：:The large and small subunits of Escherichia coli glutamate synthase were isolated. The small subunit catalyzes the NH(3)-dependent synthesis of glutamate. The large subunit exhibits glutaminase activity. ...

journal_title：Journal of bacteriology

authors： Mantsala P,Zalkin H

更新日期：1976-04-01 00:00:00

abstract：:The porin composition of the Escherichia coli cell envelope was analyzed during growth at different external pHs (pHo) as a function of the acetyl phosphate (AcP) level (DeltaackA pta or ackA mutant, pyruvate or glucose as the carbon source) in the presence or absence of EnvZ. Our results indicate that the AcP level i...

journal_title：Journal of bacteriology

authors： Heyde M,Laloi P,Portalier R

更新日期：2000-01-01 00:00:00

abstract：:Succinate dehydrogenase (EC 1.3.99.1) of Micrococcus luteus was selectively precipitated from Triton X-100-solubilized membranes by using specific antiserum. The precipitated enzyme contained equimolar amounts of four polypeptides with apparent molecular weights of 72,000, 30,000, 17,000, and 15,000. The 72,000 polype...

journal_title：Journal of bacteriology

authors： Crowe BA,Owen P

更新日期：1983-03-01 00:00:00

abstract：:Germination and outgrowth of ascospores of Saccharomyces cerevisiae 4579 require both methionine and adenine, whereas leucine is only required for outgrowth. The methionine requirement may be satisfied by S-adenosylmethionine, but this sulfonium compound will not substitute for adenine. Between 30 and 70 min of protei...

journal_title：Journal of bacteriology

authors： Choih SJ,Ferro AJ,Shapiro SK

更新日期：1977-07-01 00:00:00

abstract：:Two solid medium formulations, designated 100:10 and 10:10, were developed for the growth of Thiobacillus ferrooxidans. The new media contain a mixture of both ferrous iron and thiosulfate as available energy sources, permitting the detection of colony morphology variants that arise spontaneously in a wild-type popula...

journal_title：Journal of bacteriology

authors： Schrader JA,Holmes DS

更新日期：1988-09-01 00:00:00

abstract：:Small cytoplasmic RNA (scRNA; 271 nucleotides) is an abundant and stable RNA of the gram-positive bacterium Bacillus subtilis. To investigate the function of scRNA in B. subtilis cells, we developed a strain that is dependent on isopropyl-beta-D-thiogalactopyranoside for scRNA synthesis by fusing the chromosomal scr l...

journal_title：Journal of bacteriology

authors： Nakamura K,Imai Y,Nakamura A,Yamane K

更新日期：1992-04-01 00:00:00

abstract：:A new alginate regulatory gene, algQ, was identified in a chromosomal region which, when tandemly amplified, induces mucoidy in Pseudomonas aeruginosa. The algQ gene was found closely linked to the previously identified algR gene. Both algQ and algR were required for transcription of the key alginate biosynthetic gene...

journal_title：Journal of bacteriology

authors： Deretic V,Konyecsni WM

更新日期：1989-07-01 00:00:00

abstract：:Three genes were shown to provide functions specific for ferric enterobactin transport in Escherichia coli: fepA encoded the outer membrane receptor, fepB produced a periplasmic protein, and the fepC product was presumably a component of a cytoplasmic membrane permease system for this siderophore. A 10.6-kilobase-pair...

journal_title：Journal of bacteriology

authors： Ozenberger BA,Nahlik MS,McIntosh MA

更新日期：1987-08-01 00:00:00

abstract：:An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. The alterations observed in time course experiments were related to absorbance changes and to release of macrom...

journal_title：Journal of bacteriology

authors： Woldringh CL,van Iterson W

更新日期：1972-09-01 00:00:00

abstract：:Genetically disabled lambda plac5 transducing phage derivatives were used to study the recB dependence of recombination during specialized transduction. The frequency of transduction was normalized to colony-forming units, and the end product of recombination was monitored by scoring for addition and substitution tran...

journal_title：Journal of bacteriology

authors： Porter RD,Welliver RA,Witkowski TA

更新日期：1982-06-01 00:00:00

abstract：:The degree to which deoxyribonucleic acid segregates nonrandomly has been investigated for Escherichia coli B/r growing in different media. The degree of nonrandom segregation observed is dependent on the medium, with segregation becoming less random as the growth rate decreases. This indicates that there must be some...

journal_title：Journal of bacteriology

authors： Cooper S,Weinberger M

更新日期：1977-04-01 00:00:00

abstract：:In vivo assembly of the Klebsiella aerogenes urease nickel metallocenter requires the presence of UreD, UreF, and UreG accessory proteins and is further facilitated by UreE. Prior studies had shown that urease apoprotein exists in an uncomplexed form as well as in a series of UreD-urease (I.-S. Park, M.B. Carr, and R....

journal_title：Journal of bacteriology

authors： Moncrief MB,Hausinger RP

更新日期：1996-09-01 00:00:00

abstract：:The chb operon of Escherichia coli is involved in the utilization of the β-glucosides chitobiose and cellobiose. The function of chbG (ydjC), the sixth open reading frame of the operon that codes for an evolutionarily conserved protein is unknown. We show that chbG encodes a monodeacetylase that is essential for growt...

journal_title：Journal of bacteriology

authors： Verma SC,Mahadevan S

更新日期：2012-09-01 00:00:00

abstract：:Phosphate import is required for the growth of mycobacteria and is regulated by environmental inorganic phosphate (P(i)) concentrations, although the mechanism of this regulation has not been characterized. The expression of genes involved in P(i) acquisition is frequently regulated by two-component regulatory systems...

journal_title：Journal of bacteriology

authors： Glover RT,Kriakov J,Garforth SJ,Baughn AD,Jacobs WR Jr

更新日期：2007-08-01 00:00:00

abstract：:The expression of any given bacterial protein is predicted to depend on (i) the transcriptional regulation of the promoter and the translational regulation of its mRNA and (ii) the synthesis and translation of total (bulk) mRNA. This is because total mRNA acts as a competitor to the specific mRNA for the binding of in...

journal_title：Journal of bacteriology

authors： Liang ST,Xu YC,Dennis P,Bremer H

更新日期：2000-06-01 00:00:00

abstract：:Proteins induced by acid or base, during long-term aerobic or anaerobic growth in complex medium, were identified in Escherichia coli. Two-dimensional gel electrophoresis revealed pH-dependent induction of 18 proteins, nine of which were identified by N-terminal sequencing. At pH 9, tryptophan deaminase (TnaA) was ind...

journal_title：Journal of bacteriology

authors： Blankenhorn D,Phillips J,Slonczewski JL

更新日期：1999-04-01 00:00:00

abstract：:Both fi(+) and fi(-) type R factors function as intact replicative units when segregated into Escherichia coli minicells. Hybridization studies demonstrate that at least 95% of the deoxyribonucleic acid (DNA) in R(+) minicells is episomal in origin. About half of the DNA can be extracted in a closed circular form and ...

journal_title：Journal of bacteriology

authors： Levy SB

更新日期：1971-10-01 00:00:00

abstract：:The mevalonate diphosphate decarboxylase is an enzyme which converts mevalonate diphosphate to isopentenyl diphosphate, the building block of isoprenoids. We used the Saccharomyces cerevisiae temperature-sensitive mutant defective for mevalonate diphosphate decarboxylase previously described (C. Chambon, V. Ladeveve, ...

journal_title：Journal of bacteriology

authors： Bergès T,Guyonnet D,Karst F

更新日期：1997-08-01 00:00:00

abstract：:Virulent bacteria of the genus Yersinia secrete a number of virulence determinants called Yops. These proteins lack typical signal sequences and are not posttranslationally processed. Two gene loci have been identified as being involved in the specific Yop secretion system (G. Cornelis, p. 231-265, In C. E. Hormache, ...

journal_title：Journal of bacteriology

authors： Bergman T,Erickson K,Galyov E,Persson C,Wolf-Watz H

更新日期：1994-05-01 00:00:00

abstract：:In Pseudomonas aeruginosa, flagellar genes are regulated in a cascade headed by FleQ, an NtrC/NifA-type activator. FleQ and RpoN positively regulate expression of flhA, fliE, fliL, and fleSR genes, among others. Direct interaction of FleQ with flhA, fliE, fliL, and fleSR promoters was demonstrated by gel shift assay, ...

journal_title：Journal of bacteriology

authors： Jyot J,Dasgupta N,Ramphal R

更新日期：2002-10-01 00:00:00

abstract：:A novel regulatory gene, trh, which is involved in hrp gene expression, is identified in the plant pathogen Xanthomonas oryzae pv. oryzae. In the trh mutant, expression of HrpG, which is a key regulator for hrp gene expression, is reduced both under the in vitro hrp-inducing condition and in planta. ...

journal_title：Journal of bacteriology

authors： Tsuge S,Nakayama T,Terashima S,Ochiai H,Furutani A,Oku T,Tsuno K,Kubo Y,Kaku H

更新日期：2006-06-01 00:00:00

abstract：:A comparison of the spectra of spontaneous growth-dependent and adaptive mutations in ebgR shows that both spectra are dominated by insertion sequence (IS)-mediated mutations. The difference between growth-dependent mutations (61% IS mediated) and adaptive mutations (80% IS mediated) is highly significant (P < 0.0001)...

journal_title：Journal of bacteriology

authors： Hall BG

更新日期：1999-02-01 00:00:00

abstract：:Rhodobacter sphaeroides maintained intracellular ammonium pools of 1.1 to 2.6 mM during growth in several fixed nitrogen sources as well as during diazotrophic growth. Addition of 0.15 mM NH4+ to washed, nitrogen-free cell suspensions was followed by linear uptake of NH4+ from the medium and transient formation of int...

journal_title：Journal of bacteriology

authors： Cordts ML,Gibson J

更新日期：1987-04-01 00:00:00

abstract：UNLABELLED:Ileal lesions of patients with Crohn's disease are colonized by adherent-invasive Escherichia coli (AIEC), which is able to adhere to and to invade intestinal epithelial cells (IEC), to replicate within macrophages, and to form biofilms on the surface of the intestinal mucosa. Previous analyses indicated the...

journal_title：Journal of bacteriology

authors： Chassaing B,Garénaux E,Carriere J,Rolhion N,Guérardel Y,Barnich N,Bonnet R,Darfeuille-Michaud A

更新日期：2015-04-01 00:00:00

abstract：:A total of 508 random clones from five Mycoplasma genitalium genomic libraries were partially sequenced and analyzed. This resulted in the identification of 291 unique contigs. Sequence information from these clones (100,993 nucleotides), representing approximately 17% of this pathogen's genome, was analyzed by compar...

journal_title：Journal of bacteriology

authors： Peterson SN,Hu PC,Bott KF,Hutchison CA 3rd

更新日期：1993-12-01 00:00:00

abstract：:Heme A, as a prosthetic group, is found exclusively in respiratory oxidases of mitochondria and aerobic bacteria. Bacillus subtilis CtaA and other heme A synthases catalyze the conversion of a methyl side group on heme O into a formyl group. The catalytic mechanism of heme A synthase is not understood, and little is k...

journal_title：Journal of bacteriology

authors： Hederstedt L,Lewin A,Throne-Holst M

更新日期：2005-12-01 00:00:00

abstract：:Sphingomonas sp. strain ATCC 31555 can produce an anionic heteropolysaccharide, welan gum, which shows excellent stability and viscosity retention even at high temperatures. Here we present a 4.0-Mb assembly of its genome sequence. We have annotated 10 coding sequences (CDSs) responsible for the welan gum biosynthesis...

journal_title：Journal of bacteriology

authors： Wang X,Tao F,Gai Z,Tang H,Xu P

更新日期：2012-11-01 00:00:00

abstract：:In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by co...

journal_title：Journal of bacteriology

authors： Wong PK,Popham D,Keener J,Kustu S

更新日期：1987-06-01 00:00:00

abstract：:A combined physical and genetic map of the Serpulina hyodysenteriae B78T genome was constructed by using pulsed-field gel electrophoresis and DNA blot hybridizations. The S. hyodysenteriae genome is a single circular chromosome about 3.2 Mb in size. The physical map of the chromosome was constructed with the restricti...

journal_title：Journal of bacteriology

authors： Zuerner RL,Stanton TB

更新日期：1994-02-01 00:00:00

abstract：:We showed previously that the outer membrane of the Escherichia coli cell envelope normally contains about 200 to 250 B12 receptors, and that these receptors function both in B12 transport and as receptors for the E colicins. This paper shows that this receptor system is also shared with bacteriophage BF23. A strong p...

journal_title：Journal of bacteriology

authors： Bradbeer C,Woodrow ML,Khalifah LI

更新日期：1976-03-01 00:00:00