Abstract:
:Small cytoplasmic RNA (scRNA; 271 nucleotides) is an abundant and stable RNA of the gram-positive bacterium Bacillus subtilis. To investigate the function of scRNA in B. subtilis cells, we developed a strain that is dependent on isopropyl-beta-D-thiogalactopyranoside for scRNA synthesis by fusing the chromosomal scr locus with the spac-1 promoter by homologous recombination. Depletion of the inducer leads to a loss of scRNA synthesis, defects in protein synthesis and production of alpha-amylase and beta-lactamase, and eventual cell death. The loss of the scRNA gene in B. subtilis can be complemented by the introduction of human signal recognition particle 7S RNA, which is considered to be involved in protein transport, or Escherichia coli 4.5S RNA. These results provide further evidence for a functional relationship between B. subtilis scRNA, human signal recognition particle 7S RNA, and E. coli 4.5S RNA.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Nakamura K,Imai Y,Nakamura A,Yamane Kdoi
10.1128/jb.174.7.2185-2192.1992keywords:
subject
Has Abstractpub_date
1992-04-01 00:00:00pages
2185-92issue
7eissn
0021-9193issn
1098-5530journal_volume
174pub_type
杂志文章abstract::Among 120 strains of gliding bacteria which were screened for restriction endonucleases, 27 were found positive. Additionally, three strains carried enzymes able to release the supercoiled state of closed circular DNA. By using a new rapid method, restriction endonuclease activity was released by stirring about 0.5 g ...
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更新日期:1978-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.6.1982-1990.1968
更新日期:1968-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.150.1.410-413.1982
更新日期:1982-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.8.2724-2728.1992
更新日期:1992-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2018-05-09 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00464-08
更新日期:2008-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-02-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1715-1720.1988
更新日期:1988-04-01 00:00:00
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更新日期:2005-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.7.3329-3339.1987
更新日期:1987-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.156.2.625-635.1983
更新日期:1983-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1993-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.20.5714-5722.2002
更新日期:2002-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.3.655-658.1971
更新日期:1971-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1986-02-01 00:00:00