In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae.

abstract：:The gene coding for the Escherichia coli enzyme 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase has been cloned and sequenced. This gene, designated folK, codes for a protein of 159 amino acids, including an amino-terminal methionine. The protein was overexpressed in E. coli MC4100 by cloning the gene behind the l...

journal_title：Journal of bacteriology

authors： Talarico TL,Ray PH,Dev IK,Merrill BM,Dallas WS

更新日期：1992-09-01 00:00:00

abstract：:The BacA protein is essential for the long-term survival of Sinorhizobium meliloti and Brucella abortus within acidic compartments in plant and animal cells, respectively. Since both the S. meliloti and B. abortus bacA mutants have an increased resistance to bleomycin, it was hypothesized that BacA was a transporter o...

journal_title：Journal of bacteriology

authors： Ferguson GP,Roop RM 2nd,Walker GC

更新日期：2002-10-01 00:00:00

abstract：:The elongation factor Tu, encoded by tuf genes, is a GTP binding protein that plays a central role in protein synthesis. One to three tuf genes per genome are present, depending on the bacterial species. Most low-G+C-content gram-positive bacteria carry only one tuf gene. We have designed degenerate PCR primers derive...

journal_title：Journal of bacteriology

authors： Ke D,Boissinot M,Huletsky A,Picard FJ,Frenette J,Ouellette M,Roy PH,Bergeron MG

更新日期：2000-12-01 00:00:00

abstract：:From several presumably pure cultures of Thiobacillus ferrooxidans, we isolated a pair of stable phenotypes. One was a strict autotroph utilizing sulfur or ferrous iron as the energy source and unable to utilize glucose; the other phenotype was an acidophilic obligate heterotroph capable of utilizing glucose but not s...

journal_title：Journal of bacteriology

authors： Harrison AP Jr,Jarvis BW,Johnson JL

更新日期：1980-07-01 00:00:00

abstract：:A new strategy was developed for rapid cloning of genes with a transposon mutation library. We constructed a transposon designated TnV that was derived from Tn5 and consists of the gene coding for neomycin phosphotransferase II as well as the replication origin of an Escherichia coli plasmid, pSC101, flanked by Tn5 in...

journal_title：Journal of bacteriology

authors： Furuichi T,Inouye M,Inouye S

更新日期：1985-10-01 00:00:00

abstract：:The global transcriptional regulator Spx of Bacillus subtilis is controlled at several levels of the gene expression process. It is maintained at low concentrations during unperturbed growth by the ATP-dependent protease ClpXP. Under disulfide stress, Spx concentration increases due in part to a reduction in ClpXP-cat...

journal_title：Journal of bacteriology

authors： Garg SK,Kommineni S,Henslee L,Zhang Y,Zuber P

更新日期：2009-02-01 00:00:00

abstract：:Pseudomonas savastanoi pv. savastanoi strains harbor native plasmids belonging to the pPT23A plasmid family (PFPs) which are detected in all pathovars of the related species Pseudomonas syringae examined and contribute to the ecological and pathogenic fitness of their host. However, there is a general lack of informat...

journal_title：Journal of bacteriology

authors： Pérez-Martínez I,Zhao Y,Murillo J,Sundin GW,Ramos C

更新日期：2008-01-01 00:00:00

abstract：:Bacitracin is a peptide antibiotic nonribosomally produced by Bacillus licheniformis. The bcrABC genes which confer bacitracin resistance to the bacitracin producer encode ATP binding cassette (ABC) transporter proteins, which are hypothesized to pump out bacitracin from the cells. Bacillus subtilis 168, which has no ...

journal_title：Journal of bacteriology

authors： Ohki R,Tateno K,Okada Y,Okajima H,Asai K,Sadaie Y,Murata M,Aiso T

更新日期：2003-01-01 00:00:00

abstract：:Pseudomonas sp. isolated by selective culture with 3-phenylbutyrate (3-PB) as the sole carbon source metabolized the compound through two different pathways by initial oxidation of the benzene ring and by initial oxidation of the side chain. During early exponential growth, a catechol substance identified as 3-(2,3-di...

journal_title：Journal of bacteriology

authors： Sariaslani FS,Sudmeier JL,Focht DD

更新日期：1982-10-01 00:00:00

abstract：:Glycerol:NAD+2-OXIDOREDUCTASE (EC 1.1.1.6) was purified to homogeneity from a mutant of Escherichia coli K12 that uses this enzyme, instead of ATP:glycerol 3-phosphotransferase (EC 2.7.1.30), as the first enzyme for the dissimilation of glycerol. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sul...

journal_title：Journal of bacteriology

authors： Tang CT,Ruch FE Jr,Lin CC

更新日期：1979-10-01 00:00:00

abstract：:The gene (hmgA) for 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (EC 1.1.1.34) from the thermophilic archaeon Sulfolobus solfataricus P2 was cloned and sequenced. S. solfataricus HMG-CoA reductase exhibited a high degree of sequence identity (47%) to the HMG-CoA reductase of the halophilic archaeon Halofe...

journal_title：Journal of bacteriology

authors： Bochar DA,Brown JR,Doolittle WF,Klenk HP,Lam W,Schenk ME,Stauffacher CV,Rodwell VW

更新日期：1997-06-01 00:00:00

abstract：:By the use of [5'-32P]tRNA3Gly from Escherichia coli as a hybridization probe, glyW was located on cloned fragments of the uvrC pgsA region of the bacterial chromosome. After determination of the sites of action of several restriction enzymes, glyW was found to be within approximately 300 base pairs of pgsA. The order...

journal_title：Journal of bacteriology

authors： Tucker SD,Gopalakrishnan AS,Bollinger R,Dowhan W,Murgola EJ

更新日期：1982-11-01 00:00:00

abstract：:Rhythmically advancing waves of cells, called ripples, arise spontaneously during the aggregation of Myxococcus xanthus into fruiting bodies. Extracts prepared by washing rippling cells contain a substance that will induce quiescent cells to ripple. Three lines of evidence indicate that murein (peptidoglycan) is the r...

journal_title：Journal of bacteriology

authors： Shimkets LJ,Kaiser D

更新日期：1982-10-01 00:00:00

abstract：:Several spontaneous cya and crp mutants of Escherichia coli have been selected as clones simultaneously resistant to phage lambda and nalidixic acid and characterized. Both cya and crp mutants have been found to grow as cocci with increased doubling times. They have increased resistance to some mutagens (methylmethane...

journal_title：Journal of bacteriology

authors： Kumar S

更新日期：1976-02-01 00:00:00

abstract：:Small subunit rRNA sequences have been determined for 10 of the most clinically important pathogenic species of the yeast genus Candida (including Torulopsis [Candida] glabrata and Yarrowia [Candida] lipolytica) and for Hansenula polymorpha. Phylogenetic analyses of these sequences and those of Saccharomyces cerevisia...

journal_title：Journal of bacteriology

authors： Barns SM,Lane DJ,Sogin ML,Bibeau C,Weisburg WG

更新日期：1991-04-01 00:00:00

abstract：:Vibrio cholerae is a highly motile bacterium which possesses a single polar flagellum as a locomotion organelle. Motility is thought to be an important factor for the virulence of V. cholerae. The genome sequencing project of this organism is in progress, and the genes that are highly homologous to the essential genes...

journal_title：Journal of bacteriology

authors： Kojima S,Yamamoto K,Kawagishi I,Homma M

更新日期：1999-03-01 00:00:00

abstract：:A conjugation system initially discovered in beta-lactamase-producing gonococci mobilized small non-selftransmissible R plasmids encoding beta-lactamase (penicillinase) production into other gonococci, Neisseria, and Escherichia coli. This conjugation system was mediated by a separate selftransmissible plasmid of 23.9...

journal_title：Journal of bacteriology

authors： Sox TE,Mohammed W,Blackman E,Biswas G,Sparling PF

更新日期：1978-04-01 00:00:00

abstract：:Immunity to leptospirosis is principally humorally mediated and involves opsonization of leptospires for phagocytosis by macrophages and neutrophils. The only protective antigen identified to date is the leptospiral lipopolysaccharide (LPS), which biochemically resembles typical gram-negative LPS but has greatly reduc...

journal_title：Journal of bacteriology

authors： Mitchison M,Bulach DM,Vinh T,Rajakumar K,Faine S,Adler B

更新日期：1997-02-01 00:00:00

abstract：:The genes for protein synthesis elongation factors Tu and G were cloned from the cyanobacterium Anacystis nidulans. The locations of these genes were mapped within the cloned DNA fragment by hybridization with Escherichia coli probes. The organization of the cloned fragment and the DNA flanking it in the A. nidulans c...

journal_title：Journal of bacteriology

authors： Mickel FS,Spremulli LL

更新日期：1986-04-01 00:00:00

abstract：:Thirteen different serotypes of Listeria monocytogenes can be distinguished on the basis of variation in somatic and flagellar antigens. Although the known virulence genes are present in all serotypes, greater than 90% of human cases of listeriosis are caused by serotypes 1/2a, 1/2b, and 4b and nearly all outbreaks of...

journal_title：Journal of bacteriology

authors： Zhang C,Zhang M,Ju J,Nietfeldt J,Wise J,Terry PM,Olson M,Kachman SD,Wiedmann M,Samadpour M,Benson AK

更新日期：2003-09-01 00:00:00

abstract：:Considerable amounts of immunoglobulin G (IgG) antibody appeared in hyperimmune rabbit serum at a late period during a course of immunization with several injections of Shigella flexneri O antigens. High yields of IgG antibody possessing homogenous specificity could be fractionated from crude gamma-globulin solution o...

journal_title：Journal of bacteriology

authors： Fukazawa Y,Shinoda T,Tsuchiya T

更新日期：1969-06-01 00:00:00

abstract：:When the crude ribosomal fraction of Saccharomyces cerevisiae was separated into "light" and "heavy" fractions, fatty acid synthetase was concentrated in the former, whereas acetyl-Coenzyme A synthetase, fatty acid "desaturase," and squalene oxidocyclase were found in the latter. The "desaturase" sedimented with the r...

journal_title：Journal of bacteriology

authors： Klein HP,Volkmann CM,Leaffer MA

更新日期：1967-07-01 00:00:00

abstract：:The developmentally regulated gene dofA, identified from pulse-labeling experiments by two-dimensional gel electrophoresis, and its homologue, dofB, were cloned and characterized in Myxococcus xanthus. Deletion of dofA and dofB did not affect the vegetative growth and development of M. xanthus. dofA was specifically e...

journal_title：Journal of bacteriology

authors： Horiuchi T,Akiyama T,Inouye S,Komano T

更新日期：2002-12-01 00:00:00

abstract：:Southern blot analysis indicated that the gene encoding the constitutive, NADP-linked bile acid 7 alpha-hydroxysteroid dehydrogenase of Eubacterium sp. strain VPI 12708 was located on a 6.5-kb EcoRI fragment of the chromosomal DNA. This fragment was cloned into bacteriophage lambda gt11, and a 2.9-kb piece of this ins...

journal_title：Journal of bacteriology

authors： Baron SF,Franklund CV,Hylemon PB

更新日期：1991-08-01 00:00:00

abstract：:The accumulation of imidazoleglycerol phosphate during growth of Neurospora crassa in the presence of 3-amino-1,2,4-triazole was found to cause derepression of tryptophan synthetase and to inhibit the induction of kynureninase. Accumulation of indoleglycerol phosphate in response to growth in the presence of indole ac...

journal_title：Journal of bacteriology

authors： Turner JR,Matchett WH

更新日期：1968-05-01 00:00:00

abstract：:FtsZ and ZipA are essential components of the septal ring apparatus, which mediates cell division in Escherichia coli. FtsZ is a cytoplasmic tubulin-like GTPase that forms protofilament-like homopolymers in vitro. In the cell, the protein assembles into a ring structure at the prospective division site early in the di...

journal_title：Journal of bacteriology

authors： Hale CA,Rhee AC,de Boer PA

更新日期：2000-09-01 00:00:00

abstract：:Most motile bacteria are able to bias their movement towards more favorable environments or to escape from obnoxious substances by a process called chemotaxis. Chemotaxis depends on a chemosensory system that is able to sense specific environmental signals and generate a behavioral response. Typically, the signal is t...

journal_title：Journal of bacteriology

authors： Ringgaard S,Yang W,Alvarado A,Schirner K,Briegel A

更新日期：2018-03-12 00:00:00

abstract：:Lipopolysaccharide (LPS) was isolated from the unicellular blue-green bacterium Agmenellum quadruplicatum using the procedure of Westphal and Jann (1965). It was composed of a lipid A and polysaccharide region suggesting a similarity to other gram-negative LPSs. Chemical analyses demonstrated the presence of glucose, ...

journal_title：Journal of bacteriology

authors： Buttke TM,Ingram LO

更新日期：1975-12-01 00:00:00

abstract：:The Dps-like peroxide resistance protein (Dpr) is essential for H2O2 stress tolerance and aerobic growth of the oral pathogen Streptococcus mutans Dpr accumulates during oxidative stress, protecting the cell by sequestering iron ions and thereby preventing the generation of toxic hydroxyl radicals that result from the...

journal_title：Journal of bacteriology

authors： Ganguly T,Kajfasz JK,Miller JH,Rabinowitz E,Galvão LCC,Rosalen PL,Abranches J,Lemos JA

更新日期：2018-06-25 00:00:00

abstract：:The mutB gene of Salmonella typhimurium is involved in a methylation-independent repair pathway specific for A/G or A/C mismatches and is the homolog of the Escherichia coli mutY gene. The mutB gene of S. typhimurium was cloned and sequenced. The isolated mutB clone reduced the mutation rate of the mutB mutant to wild...

journal_title：Journal of bacteriology

authors： Desiraju V,Shanabruch WG,Lu AL

更新日期：1993-01-01 00:00:00