Novel one-step cloning vector with a transposable element: application to the Myxococcus xanthus genome.

abstract：:The capsular polysaccharide is a critical virulence factor for group B streptococci associated with human infections, yet little is known about capsule biosynthesis. We detected CMP-Neu5Ac synthetase, the enzyme which activates N-acetylneuraminic acid (Neu5Ac, or sialic acid) for transfer to the nascent capsular polys...

journal_title：Journal of bacteriology

authors： Haft RF,Wessels MR

更新日期：1994-12-01 00:00:00

abstract：:In vitro complementation assays have been used to demonstrate the induction of alkane hydroxylase proteins in mutants lacking the ability to convert n-alkanes to their primary alcohols. Purified heptane is an effective inducer in a mutant lacking detectable hydroxylase activity. ...

journal_title：Journal of bacteriology

authors： Benson S,Shapiro J

更新日期：1975-08-01 00:00:00

abstract：:The asg mutants of Myxococcus xanthus are defective in production of extracellular A-signal, which serves as a cell density signal for fruiting-body development. The DNA sequence of asgB, one of the three asg genes, was determined. The deduced amino acid sequence of AsgB contains a DNA-binding helix-turn-helix motif n...

journal_title：Journal of bacteriology

authors： Plamann L,Davis JM,Cantwell B,Mayor J

更新日期：1994-04-01 00:00:00

abstract：:We have determined the amounts of a number of small molecules and enzymes in the mother cell compartment and the developing forespore during sporulation of Bacillus megaterium. Significant amounts of adenosine 5'-triphosphate and reduced nicotinamide adenine dinucleotide were present in the forespore compartment befor...

journal_title：Journal of bacteriology

authors： Singh RP,Setlow B,Setlow P

更新日期：1977-06-01 00:00:00

abstract：:A 6.9-kilobase region of the Escherichia coli F plasmid containing the 3' half of the traD gene and the entire traI gene (encodes the TraI protein, DNA helicase I and TraI, a polypeptide arising from an internal in-frame translational start in traI) has been sequenced. A previously unidentified open reading frame (ten...

journal_title：Journal of bacteriology

authors： Bradshaw HD Jr,Traxler BA,Minkley EG Jr,Nester EW,Gordon MP

更新日期：1990-07-01 00:00:00

abstract：:The fatty acid pattern in three hydrocarbon-utilizing bacteria during growth on various substrates was examined. The predominant fatty acids in acetate-grown cells were C(16), C(16:1), C(18:1), and Br-C(19) and the major fatty acids in propane-grown cells were C(15), C(17), C(17:1), C(18:1), and Br-C(18). When one org...

journal_title：Journal of bacteriology

authors： Dunlap KR,Perry JJ

更新日期：1967-12-01 00:00:00

abstract：:A sulfonic acid found to be a major constituent of spores of Bacillus subtilis was provisionally identified as 3-l-sulfolactic acid. This compound was completely absent from vegetative cells during growth, but large amounts accumulated in sporulating cells just before the development of refractile spores. Essentially ...

journal_title：Journal of bacteriology

authors： Bonsen PP,Spudich JA,Nelson DL,Kornberg A

更新日期：1969-04-01 00:00:00

abstract：:The filamentous cyanobacterium Anabaena fixes nitrogen in specialized cells called heterocysts. The immediate product of fixation, ammonia, is known to be assimilated by addition to glutamate to make glutamine. How fixed nitrogen is transported along the filament to the 10 to 20 vegetative cells that separate heterocy...

journal_title：Journal of bacteriology

authors： Ke S,Haselkorn R

更新日期：2013-01-01 00:00:00

abstract：:Smith, Rodney F. (University of Maryland, Baltimore), Donald E. Shay, and Norman J. Doorenbos. Antimicrobial action of nitrogen-containing steroids. J. Bacteriol. 85:1295-1299. 1963.-A new group of 16 synthetic nitrogen-containing steroids have been tested against a variety of microorganisms for antimicrobial properti...

journal_title：Journal of bacteriology

authors： SMITH RF,SHAY DE,DOORENBOS NJ

更新日期：1963-06-01 00:00:00

abstract：:Vibrio cholerae uses a variety of strategies for obtaining iron in its diverse environments. In this study we report the identification of a novel iron utilization protein in V. cholerae, VciB. The vciB gene and its linked gene, vciA, were isolated in a screen for V. cholerae genes that permitted growth of an Escheric...

journal_title：Journal of bacteriology

authors： Mey AR,Wyckoff EE,Hoover LA,Fisher CR,Payne SM

更新日期：2008-09-01 00:00:00

abstract：:The seven proteins encoded by the comG operon of Bacillus subtilis exhibit similarity to gene products required for the assembly of type 4 pili and for the secretion of certain proteins in gram-negative bacteria. Although polar transposon insertions in comG result in the loss of transformability and in the failure of ...

journal_title：Journal of bacteriology

authors： Chung YS,Dubnau D

更新日期：1998-01-01 00:00:00

abstract：:Phosphate is an essential nutrient that Staphylococcus aureus and other pathogens must acquire from the host during infection. While inorganic monophosphate (Pi) is the preferred source of this nutrient, bacteria can also obtain it from phosphate-containing organic molecules. The Pi-responsive regulator PhoPR is neces...

journal_title：Journal of bacteriology

authors： Kelliher JL,Leder Macek AJ,Grudzinski KM,Radin JN,Kehl-Fie TE

更新日期：2020-10-22 00:00:00

abstract：:Sphingomonas wittichii RW1 degrades chlorinated dibenzofurans and dibenzo-p-dioxins via meta cleavage. We used inverse PCR to amplify dxnB2, a gene encoding one of three meta-cleavage product (MCP) hydrolases identified in the organism that are homologues of BphD involved in biphenyl catabolism. Purified DxnB2 catalyz...

journal_title：Journal of bacteriology

authors： Seah SY,Ke J,Denis G,Horsman GP,Fortin PD,Whiting CJ,Eltis LD

更新日期：2007-06-01 00:00:00

abstract：:Purified superdormant spores of Bacillus cereus, B. megaterium, and B. subtilis isolated after optimal heat activation of dormant spores and subsequent germination with inosine, d-glucose, or l-valine, respectively, germinate very poorly with the original germinants used to remove dormant spores from spore populations...

journal_title：Journal of bacteriology

authors： Ghosh S,Zhang P,Li YQ,Setlow P

更新日期：2009-09-01 00:00:00

abstract：:A chemically defined medium (MI(c)) is described in which cells of Haemophilus influenzae Rd grow rapidly (generation time, 35 +/- 5 min) and reach a stationary level of 10(10) cells/ml. Our strain of cells grown in this medium developed high levels of competence when transferred to another medium designed for that pu...

journal_title：Journal of bacteriology

authors： Herriott RM,Meyer EY,Vogt M,Modan M

更新日期：1970-02-01 00:00:00

abstract：:Cells of the osmotolerant yeast Saccharomyces rouxii were transformed to protoplasts in good yield (85%) by digesting cell walls with snail-gut enzyme in the presence of 10 mM dithioerythritol, 0.1 M sodium phosphate buffer (pH 6.8), and 2.0 M KCl. The requirement for 2.0 M KCl compares with that for S. bisporus var. ...

journal_title：Journal of bacteriology

authors： Arnold WN,Garrison RG

更新日期：1979-03-01 00:00:00

abstract：:Fructose-6-phosphate kinase (pfkA) mutants have impaired growth on carbon sources which enter glycolysis at or above the level of fructose-6-phosphate, but the degree of impairment depends on the carbon source (e.g., growth on glucose is very much slower than growth on glucose-6-phosphate). The present report contains...

journal_title：Journal of bacteriology

authors： Vinopal RT,Fraenkel DG

更新日期：1974-06-01 00:00:00

abstract：:The activity of bacterial alkaline phosphatase (PhoA) is dependent on it being exported across the plasma membrane. A plasmid vector (pJEM11) allowing fusions between phoA and genes encoding exported proteins was constructed to study protein export in mycobacteria. Introduction of the Mycobacterium fortuitum beta-lact...

journal_title：Journal of bacteriology

authors： Lim EM,Rauzier J,Timm J,Torrea G,Murray A,Gicquel B,Portnoi D

更新日期：1995-01-01 00:00:00

abstract：:The mechanism of action of diphtheria toxin in an Escherichia coli cell-free protein-synthesizing system was examined. When the washed ribosomes were incubated with toxin before addition of messenger ribonucleic acid (RNA), peptide syntheses of (14)C-phenylalanine directed by polyuridylic acid or phage R17 RNA were st...

journal_title：Journal of bacteriology

authors： Tsugawa A,Ohsumi Y,Kato I

更新日期：1970-10-01 00:00:00

abstract：:The mecA-27r gene from Staphylococcus aureus 27r encodes penicillin-binding protein 2a (PBP2a-27r), which causes this strain to be methicillin resistant. Removal or replacement of the N-terminal transmembrane domain had no effect on binding of penicillin, but removal of portions of the putative transglycosylase domain...

journal_title：Journal of bacteriology

authors： Wu CY,Alborn WE Jr,Flokowitsch JE,Hoskins J,Unal S,Blaszczak LC,Preston DA,Skatrud PL

更新日期：1994-01-01 00:00:00

abstract：:We previously described a bacteriophage of the Lyme disease agent Borrelia burgdorferi designated phiBB-1. This phage packages the host complement of the 32-kb circular plasmids (cp32s), a group of homologous molecules found throughout the genus Borrelia. To demonstrate the ability of phiBB-1 to package and transduce ...

journal_title：Journal of bacteriology

authors： Eggers CH,Kimmel BJ,Bono JL,Elias AF,Rosa P,Samuels DS

更新日期：2001-08-01 00:00:00

abstract：:The regulatory proteins OmpR and EnvZ are both required to activate expression of the genes for the major outer membrane porin proteins, OmpF and OmpC, of Escherichia coli K-12. Here we show that OmpR, under certain conditions, could activate porin expression in the complete absence of EnvZ. In addition, the pleiotrop...

journal_title：Journal of bacteriology

authors： Slauch JM,Garrett S,Jackson DE,Silhavy TJ

更新日期：1988-01-01 00:00:00

abstract：:Aerosols of Mycoplasma pneumoniae were studied at several relative humidities at a controlled temperature of 27 C. Production of an experimentally reproducible aerosol required preatomization of the organism in its suspending fluid and was dependent on the type of fluid used in atomization as well as on the procedures...

journal_title：Journal of bacteriology

authors： Wright DN,Bailey GD,Hatch MT

更新日期：1968-10-01 00:00:00

abstract：:After the demonstration that Stigmatella aurantiaca DW4 secretes an endo-N-acetyl-beta-D-glucosaminidase (ENGase), acting on the di-N-acetylchitobiosyl part of N-linked glycans (S. Bourgerie, Y. Karamanos, T. Grard, and R. Julien, J. Bacteriol. 176:6170-6174, 1994), an ENGase activity having the same substrate specifi...

journal_title：Journal of bacteriology

authors： Barreaud JP,Bourgerie S,Julien R,Guespin-Michel JF,Karamanos Y

更新日期：1995-02-01 00:00:00

abstract：:We have used a plasmid-based transcriptional reporter system to examine the transcriptional effects of 33 single point mutations in the box A region (TATA-like sequence) of the Haloferax volcanii tRNA(Lys) promoter. The most pronounced effects on transcriptional efficiency were found when the nucleotides corresponding...

journal_title：Journal of bacteriology

authors： Palmer JR,Daniels CJ

更新日期：1995-04-01 00:00:00

abstract：:Resting cells of Escherichia coli are able to initiate growth and murein biosynthesis in the presence of beta-lactam antibiotics binding to penicillin-binding proteins (PBPs) 1a and 1b (E. J. de la Rosa, M. A. de Pedro, and D. Vázquez, Proc. Natl. Acad. Sci. USA 82:5632-5635, 1985). Under these conditions, cells elong...

journal_title：Journal of bacteriology

authors： García-del Portillo F,Pisabarro AG,de la Rosa EJ,de Pedro MA

更新日期：1987-06-01 00:00:00

abstract：:The cell walls of selected bacteria were studied by X-ray diffraction analysis to determine and characterize crystalline components. The walls were isolated by mechanical disruption and purified by enzymatic and washing procedures. The X-ray diffraction lines which appeared from the gram-positive cell walls were shown...

journal_title：Journal of bacteriology

authors： Carito SL,Bazil SL,DiGiacomo G

更新日期：1967-01-01 00:00:00

abstract：:With two-dimensional restriction enzyme analysis we have been able to cleave the Bacillus subtilis genome and resolve the resulting deoxyribonucleic acid (DNA) segments into discrete bands on agarose gels. A general procedure for gene purification has been developed by coupling multidimensional restriction analysis wi...

journal_title：Journal of bacteriology

authors： Potter SS,Bott KF,Newbold JE

更新日期：1977-01-01 00:00:00

abstract：:The cell wall lipopolysaccharides from 17 species belonging to the genus Xanthomonas were extracted from the cells with hot 45% phenol. After purification, the components of the polysaccharide were obtained by acid hydrolysis of the lipopolysaccharide and were quantitatively assayed. Data obtained show that all prepar...

journal_title：Journal of bacteriology

authors： Volk WA

更新日期：1968-03-01 00:00:00

abstract：:Since relatively few quantitative immunological studies had been conducted with invertebrates, this study was designed to learn more about the immune potential of the oyster, a molluscan representative. Experiments measured the primary and secondary clearance rates of T2 coliphage in the oyster. The fate of phage inje...

journal_title：Journal of bacteriology

authors： Acton RT,Evans EE

更新日期：1968-04-01 00:00:00