Abstract:
:Purified superdormant spores of Bacillus cereus, B. megaterium, and B. subtilis isolated after optimal heat activation of dormant spores and subsequent germination with inosine, d-glucose, or l-valine, respectively, germinate very poorly with the original germinants used to remove dormant spores from spore populations, thus allowing isolation of the superdormant spores, and even with alternate germinants. However, these superdormant spores exhibited significant germination with the original or alternate germinants if the spores were heat activated at temperatures 8 to 15 degrees C higher than the optimal temperatures for the original dormant spores, although the levels of superdormant spore germination were not as great as those of dormant spores. Use of mixtures of original and alternate germinants lowered the heat activation temperature optima for both dormant and superdormant spores. The superdormant spores had higher wet-heat resistance and lower core water content than the original dormant spore populations, and the environment of dipicolinic acid in the core of superdormant spores as determined by Raman spectroscopy of individual spores differed from that in dormant spores. These results provide new information about the germination, heat activation optima, and wet-heat resistance of superdormant spores and the heterogeneity in these properties between individual members of dormant spore populations.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ghosh S,Zhang P,Li YQ,Setlow Pdoi
10.1128/JB.00736-09subject
Has Abstractpub_date
2009-09-01 00:00:00pages
5584-91issue
18eissn
0021-9193issn
1098-5530pii
JB.00736-09journal_volume
191pub_type
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doi:10.1128/JB.180.15.3923-3932.1998
更新日期:1998-08-01 00:00:00
abstract::A previously constructed trp-S-lacZ fusion encoding a hybrid protein with beta-galactosidase activity was subcloned from a multicopy plasmid onto a lambda vector. Single-copy lysogens of lambda trpS-lacZ were used to determine whether trpS was regulated in a manner similar to that of other aminoacyl-tRNA synthetases. ...
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更新日期:1980-09-01 00:00:00
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更新日期:1994-11-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2014-09-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:1991-06-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1999-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.172.10.5758-5766.1990
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更新日期:1980-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.2.737-741.1982
更新日期:1982-08-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:1978-06-01 00:00:00
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更新日期:2010-03-01 00:00:00
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更新日期:1983-03-01 00:00:00
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更新日期:1999-10-01 00:00:00
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更新日期:2011-09-01 00:00:00
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更新日期:1983-10-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2009-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.3620-3630.1990
更新日期:1990-07-01 00:00:00
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更新日期:2009-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-12-01 00:00:00