Exchange of genetic markers at extremely high temperatures in the archaeon Sulfolobus acidocaldarius.

abstract：:Mycoplasma mobile has a unique mechanism that enables it to glide on solid surfaces faster than any other gliding mycoplasma. To elucidate the gliding mechanism, we developed a transformation system for M. mobile based on a transposon derived from Tn4001. Modification of the electroporation conditions, outgrowth time,...

journal_title：Journal of bacteriology

authors： Tulum I,Yabe M,Uenoyama A,Miyata M

更新日期：2014-05-01 00:00:00

abstract：:Myxococcus xanthus, a gram-negative bacterium, has a complex life cycle. In response to starvation, most cells in a population participate in the formation of multicellular aggregates (i.e., fruiting bodies) in which cells differentiate into spores. However, some cells do not enter aggregates. In this and the two acco...

journal_title：Journal of bacteriology

authors： O'Connor KA,Zusman DR

更新日期：1991-06-01 00:00:00

abstract：:Freeman, B. M. (Houghton Poultry Research Station, Huntingdon, England), and L. G. Chubb. Effect of Salmonella gallinarum on certain Krebs cycle intermediates of domestic fowl. J. Bacteriol. 88:93-95. 1964.-In chickens infected with Salmonella gallinarum, the citric acid content of the blood rose but that of the liver...

journal_title：Journal of bacteriology

authors： FREEMAN BM,CHUBB LG

更新日期：1964-07-01 00:00:00

abstract：:Bacillus subtilis responds to phosphate starvation stress by inducing the PhoP and SigB regulons. While the PhoP regulon provides a specific response to phosphate starvation stress, maximizing the acquisition of phosphate (P(i)) from the environment and reducing the cellular requirement for this essential nutrient, th...

journal_title：Journal of bacteriology

authors： Allenby NE,O'Connor N,Prágai Z,Ward AC,Wipat A,Harwood CR

更新日期：2005-12-01 00:00:00

abstract：:The Tat system is a recently discovered protein export pathway that serves to translocate folded proteins, often containing redox cofactors, across the bacterial cytoplasmic membrane. Here we report that tat strains are associated with a mutant cell septation phenotype, where chains of up to 10 cells are evident. Muta...

journal_title：Journal of bacteriology

authors： Stanley NR,Findlay K,Berks BC,Palmer T

更新日期：2001-01-01 00:00:00

abstract：:When allyl alcohol was used as a suicide substrate, spontaneous mutants and UV light- and nitrous acid-generated mutants of Methylobacterium organophilum XX were selected which grew on methylamine but not on methanol. There was no detectable methanol dehydrogenase (MDH) activity in crude extracts of these mutants, yet...

journal_title：Journal of bacteriology

authors： Machlin SM,Tam PE,Bastien CA,Hanson RS

更新日期：1988-01-01 00:00:00

abstract：:The ftf gene, coding for the cell-bound beta-D-fructosyltransferase (FTF) of Streptococcus salivarius ATCC 25975, has been analyzed, and its deduced amino acid sequence has been compared with that of the secreted FTF of Streptococcus mutans and the levansucrases (SacBs) of Bacillus species. A unique proline-rich regio...

journal_title：Journal of bacteriology

authors： Rathsam C,Giffard PM,Jacques NA

更新日期：1993-07-01 00:00:00

abstract：:Nine natural isolates of Escherichia coli were examined, and the sequence of the entire 1,404 bases of the gnd gene (6-phosphogluconate dehydrogenase, EC 1.1.1.44) was determined. These isolates, along with E. coli K-12, constitute 10 strains for analysis. (The sequence of the E. coli K-12 gnd gene is known.) A total ...

journal_title：Journal of bacteriology

authors： Bisercić M,Feutrier JY,Reeves PR

更新日期：1991-06-01 00:00:00

abstract：:Mycobacterium avium-Mycobacterium intracellulare complex (MAC) is the most common isolate of nontuberculous mycobacteria and causes pulmonary and extrapulmonary diseases. MAC species can be grouped into 31 serotypes by the epitopic oligosaccharide structure of the species-specific glycopeptidolipid (GPL) antigen. The ...

journal_title：Journal of bacteriology

authors： Fujiwara N,Nakata N,Naka T,Yano I,Doe M,Chatterjee D,McNeil M,Brennan PJ,Kobayashi K,Makino M,Matsumoto S,Ogura H,Maeda S

更新日期：2008-05-01 00:00:00

abstract：:The mecA-27r gene, which encodes PBP2a-27r, was modified by site-specific mutagenesis, resulting in replacement of the N-terminal membrane anchor with a short chelating peptide (CP-PBP2a-27r). CP-PBP2a-27r retained the same binding affinity for beta-lactam antibiotics as the wild-type enzyme. Approximately 95% pure CP...

journal_title：Journal of bacteriology

authors： Wu CY,Blaszczak LC,Smith MC,Skatrud PL

更新日期：1994-03-01 00:00:00

abstract：:The Bacillus anthracis endospore loses resistance properties during germination when its cortex peptidoglycan is degraded by germination-specific lytic enzymes (GSLEs). Although this event normally employs several GSLEs for complete cortex removal, the SleB protein alone can facilitate enough cortex hydrolysis to prod...

journal_title：Journal of bacteriology

authors： Heffron JD,Sherry N,Popham DL

更新日期：2011-01-01 00:00:00

abstract：:The large virulence plasmid pMYSH6000 of Shigella flexneri contains a determinant that is highly effective in stabilizing otherwise unstable plasmids in Escherichia coli. Expression of two small contiguous genes, mvpA and mvpT (formerly termed STBORF1 and STBORF2), was shown to be sufficient for stability. Mutations i...

journal_title：Journal of bacteriology

authors： Sayeed S,Reaves L,Radnedge L,Austin S

更新日期：2000-05-01 00:00:00

abstract：:The Escherichia coli HtrA protein is a periplasmic protease/chaperone that is upregulated under stress conditions. The protease and chaperone activities of HtrA eliminate or refold damaged and unfolded proteins in the bacterial periplasm that are generated upon stress conditions. In the absence of substrates, HtrA oli...

journal_title：Journal of bacteriology

authors： Jomaa A,Iwanczyk J,Tran J,Ortega J

更新日期：2009-03-01 00:00:00

abstract：:By transformation of dnaA null mutant host cells that are suppressed either by an rnh mutation or by chromosomal integration of a mini-R1 plasmid, it was shown that replication of miniplasmids composed of the NR1 minimal replicon had no absolute dependence upon DnaA protein. In addition, the suppression of the dnaA nu...

journal_title：Journal of bacteriology

authors： Tang XB,Womble DD,Rownd RH

更新日期：1989-10-01 00:00:00

abstract：:AraC protein, a transcriptional regulator of the L-arabinose operon in Escherichia coli, is dimeric. Each monomer consists of a domain for DNA binding plus transcription activation and a domain for dimerization plus arabinose binding. These are connected to one another by a linker region of at least 5 amino acids. Her...

journal_title：Journal of bacteriology

authors： Eustance RJ,Schleif RF

更新日期：1996-12-01 00:00:00

abstract：:We show that hisT function is required for normal growth of Escherichia coli K-12, since a lack of hisT-mediated pseudouridine tRNA modification causes a uracil requirement that interferes with cell division. We also show that hisT transcription is positively growth rate regulated in exponentially growing bacteria and...

journal_title：Journal of bacteriology

authors： Tsui HC,Arps PJ,Connolly DM,Winkler ME

更新日期：1991-11-01 00:00:00

abstract：:Protein synthesis in Allomyces marcogynus mitospores began at the time of encystment and was required for further germination. The rate of protein synthesis in germinating spores decreased in the absence of ribonucleic acid synthesis relative to the uninhibited control. There was a concomitant decrease in polysome con...

journal_title：Journal of bacteriology

authors： Smith BA,Burke DD

更新日期：1980-09-01 00:00:00

abstract：:The azlB locus of Bacillus subtilis was defined previously by a mutation conferring resistance to a leucine analog, 4-azaleucine (J. B. Ward, Jr., and S. A. Zahler, J. Bacteriol. 116:727-735, 1973). In this report, azlB is shown to be the first gene of an operon apparently involved in branched-chain amino acid transpo...

journal_title：Journal of bacteriology

authors： Belitsky BR,Gustafsson MC,Sonenshein AL,Von Wachenfeldt C

更新日期：1997-09-01 00:00:00

abstract：:Siderophore-mediated iron transport in Pseudomonas aeruginosa is dependent upon the cytoplasmic membrane-associated TonB1 energy coupling protein for activity. To assess the functional significance of the various regions of this molecule and to identify functionally important residues, the tonB1 gene was subjected to ...

journal_title：Journal of bacteriology

authors： Zhao Q,Poole K

更新日期：2002-03-01 00:00:00

abstract：:A conditional developmental mutant of Mucor racemosus which is capable of oxidative energy metabolism is described. Unlike the wild-type strain the mutant was highly fermentative and exhibited the yeast morphology when grown aerobically in glucose-containing media. The high fermentative activity and yeast morphology u...

journal_title：Journal of bacteriology

authors： Borgia PT,Gokul NK,Phillips GJ

更新日期：1985-12-01 00:00:00

abstract：:Early log-phase cells of Mycoplasma gallisepticum A5969 were synchronized by holding in Eagle minimal essential medium (MEM) for 2 h. When transferred out of MEM into tryptose medium, the cells exhibited synchronous growth. Deoxyribonucleic acid (DNA) synthesis proceeded continuously during this growth but stopped dur...

journal_title：Journal of bacteriology

authors： Quinlan DC,Maniloff J

更新日期：1973-07-01 00:00:00

abstract：:Two low-molecular-weight basic proteins, termed A and B proteins, comprise about 15% of the protein of dormant spores of Bacillus megaterium. Irradiation of intact dormant spores with ultraviolet light results in covalent cross-linking of the A and B proteins to other spore macromolecules. The cross-linked A and B pro...

journal_title：Journal of bacteriology

authors： Setlow B,Setlow P

更新日期：1979-08-01 00:00:00

abstract：:We have mapped a regulatory site mediating the hyperproduction of cholera toxin in mutants of Vibrio cholerae strain 569B. Mutations in this locus, called htx, result in the hypertoxinogenic phenotype, as measured by the ganglioside filter assay and immunoradial diffusion. Transposon-facilitated recombination was used...

journal_title：Journal of bacteriology

authors： Mekalanos JJ,Sublett RD,Romig WR

更新日期：1979-09-01 00:00:00

abstract：:The O antigen is an important cell wall antigen of gram-negative bacteria, and the genes responsible for its biosynthesis are located in a gene cluster. We have cloned and sequenced the DNA segment unique to the O-antigen gene cluster of Salmonella enterica group D3. This segment includes a novel O-antigen polymerase ...

journal_title：Journal of bacteriology

authors： Curd H,Liu D,Reeves PR

更新日期：1998-02-01 00:00:00

abstract：:The intermolecular interactions of the mycobacteriophage Ms6 secretion chaperone with endolysin were characterized. The 384-amino-acid lysin (lysin(384))-binding domain was found to encompass the N-terminal region of Gp1, which is also essential for a lysis phenotype in Escherichia coli. In addition, a GXXXG-like moti...

journal_title：Journal of bacteriology

authors： Catalão MJ,Gil F,Moniz-Pereira J,Pimentel M

更新日期：2011-09-01 00:00:00

abstract：:Pseudomonas oleovorans contains an isomerase which catalyzes the cis-trans conversion of the abundant unsaturated membrane fatty acids 9-cis-hexadecenoic acid (palmitoleic acid) and 11-cis-octadecenoic acid (vaccenic acid). We purified the isomerase from the periplasmic fraction of Pseudomonas oleovorans. The molecula...

journal_title：Journal of bacteriology

authors： Pedrotta V,Witholt B

更新日期：1999-05-01 00:00:00

abstract：:The acetogenic bacterium Clostridium thermoautotrophicum, grown on methanol, glucose, or CO2-H2, contained high levels of corrinoids, formate dehydrogenase, tetrahydrofolate enzymes, carbon monoxide dehydrogenase, and hydrogenase. Cell-free extracts catalyzed pyruvate-dependent formation of acetate from methyltetrahyd...

journal_title：Journal of bacteriology

authors： Clark JE,Ragsdale SW,Ljungdahl LG,Wiegel J

更新日期：1982-07-01 00:00:00

abstract：:Covalently closed circular deoxyribonucleic acid (DNA) was isolated from two strains of Streptomyces coelicolor A3(2), representing two of the known fertility types. In each of the two strains circular DNA of about 20 times 10-6 daltons could be detected, amounting to about 1.5% of the total cellular DNA. The possible...

journal_title：Journal of bacteriology

authors： Schrempf H,Bujard H,Hopwood DA,Goebel W

更新日期：1975-02-01 00:00:00

abstract：:The minimal origin of replication of the broad-host-range plasmid RK2 has two potential recognition sequences for the DnaA protein of Escherichia coli. DNA transfer by transformation into a dnaA-null mutant of E. coli showed that DnaA protein is needed for replication or maintenance of mini-RK2. We isolated and purifi...

journal_title：Journal of bacteriology

authors： Gaylo PJ,Turjman N,Bastia D

更新日期：1987-10-01 00:00:00

abstract：:The biosynthesis of the thaxtomin cyclic dipeptide phytotoxins proceeds nonribosomally via the thiotemplate mechanism. Acyladenylation, thioesterification, N-methylation, and cyclization of two amino acid substrates are catalyzed by the txtAB-encoded thaxtomin synthetase. Nucleotide sequence analysis of the region 3' ...

journal_title：Journal of bacteriology

authors： Healy FG,Krasnoff SB,Wach M,Gibson DM,Loria R

更新日期：2002-04-01 00:00:00