The cell-bound fructosyltransferase of Streptococcus salivarius: the carboxyl terminus specifies attachment in a Streptococcus gordonii model system.

Abstract:

:The ftf gene, coding for the cell-bound beta-D-fructosyltransferase (FTF) of Streptococcus salivarius ATCC 25975, has been analyzed, and its deduced amino acid sequence has been compared with that of the secreted FTF of Streptococcus mutans and the levansucrases (SacBs) of Bacillus species. A unique proline-rich region detected at the C terminus of the FTF of S. salivarius preceded a hydrophobic terminal domain. This proline-rich region was shown to possess strong homology to the product of the prgC gene from pCF10 in Enterococcus faecalis, which encodes a pheromone-responsive protein of unknown function, as well as homology to the human proline-rich salivary protein PRP-4. A series of 3'-OH deletions of the S. salivarius ftf gene expressed in Streptococcus gordonii Challis LGR2 showed that the C terminus was required for cell surface attachment in this heterologous organism, as only the complete gene product was cell bound. This cell-bound activity was released in the presence of sucrose, suggesting that the mode of attachment and release of the S. salivarius FTF in S. gordonii was similar to that in its native host.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Rathsam C,Giffard PM,Jacques NA

doi

10.1128/jb.175.14.4520-4527.1993

subject

Has Abstract

pub_date

1993-07-01 00:00:00

pages

4520-7

issue

14

eissn

0021-9193

issn

1098-5530

journal_volume

175

pub_type

杂志文章
  • Isolation of the lipid intermediate in peptidoglycan biosynthesis from Escherichia coli.

    abstract::The lipid intermediate in peptidoglycan biosynthesis was isolated from Escherichia coli strain W and characterized as C(55)-isoprenyl-pyrophosphoryl N-acetylmuramyl(-pentapeptide)-N-acetyl-glucosamine. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.3.1306-1309.1972

    authors: Umbreit JN,Strominger JL

    更新日期:1972-12-01 00:00:00

  • Magnesium Suppresses Defects in the Formation of 70S Ribosomes as Well as in Sporulation Caused by Lack of Several Individual Ribosomal Proteins.

    abstract::Individually, the ribosomal proteins L1, L23, L36, and S6 are not essential for cell proliferation of Bacillus subtilis, but the absence of any one of these ribosomal proteins causes a defect in the formation of the 70S ribosomes and a reduced growth rate. In mutant strains individually lacking these ribosomal protein...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00212-18

    authors: Akanuma G,Yamazaki K,Yagishi Y,Iizuka Y,Ishizuka M,Kawamura F,Kato-Yamada Y

    更新日期:2018-08-24 00:00:00

  • Comparison of two ferredoxins from Rhodospirillum rubrum as electron carriers for the native nitrogenase.

    abstract::In coupling the reducing power of illuminated chloroplasts to the nitrogenase from photosynthetically grown Rhodospirillum rubrum cells, one of the native ferredoxins. FdI, was found to be three times more effective than FdII. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.2.743-745.1975

    authors: Yoch DC,Arnon DI

    更新日期:1975-02-01 00:00:00

  • Vibrio vulnificus secretes a broad-specificity metalloprotease capable of interfering with blood homeostasis through prothrombin activation and fibrinolysis.

    abstract::Vibrio vulnificus is a causative agent of serious food-borne diseases in humans related to the consumption of raw seafood. It secretes a metalloprotease that is associated with skin lesions and serious hemorrhagic complications. In this study, we purified and characterized an extracellular metalloprotease (designated ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.20.6909-6916.2005

    authors: Chang AK,Kim HY,Park JE,Acharya P,Park IS,Yoon SM,You HJ,Hahm KS,Park JK,Lee JS

    更新日期:2005-10-01 00:00:00

  • Purification and properties of pyridoxal-5'-phosphate-dependent histidine decarboxylases from Klebsiella planticola and Enterobacter aerogenes.

    abstract::Histidine decarboxylases from Klebsiella planticola and Enterobacter aerogenes were purified to homogeneity and compared with the histidine decarboxylase from Morganella morganii. All three enzymes required pyridoxal 5'-phosphate as a coenzyme, showed optimal activity at pH 6.5, decarboxylated only histidine among the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.9.3963-3968.1987

    authors: Guirard BM,Snell EE

    更新日期:1987-09-01 00:00:00

  • Adenylate energy charge in Saccharomyces cerevisiae during starvation.

    abstract::Bakers' yeast cells, Saccharomyces cerevisiae, if grown aerobically on ethanol or if grown aerobically on glucose and allowed to pass into stationary phase, with utilization of accumulated ethanol, maintain a normal value (0.8 to 0.9) of the adenylate energy charge during prolonged starvation. In contrast, cells grown...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.3.975-982.1975

    authors: Ball WJ Jr,Atkinson DE

    更新日期:1975-03-01 00:00:00

  • Kinetic characterization of the WalRKSpn (VicRK) two-component system of Streptococcus pneumoniae: dependence of WalKSpn (VicK) phosphatase activity on its PAS domain.

    abstract::The WalRK two-component system plays important roles in maintaining cell wall homeostasis and responding to antibiotic stress in low-GC Gram-positive bacteria. In the major human pathogen, Streptococcus pneumoniae, phosphorylated WalR(Spn) (VicR) response regulator positively controls the transcription of genes encodi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01690-09

    authors: Gutu AD,Wayne KJ,Sham LT,Winkler ME

    更新日期:2010-05-01 00:00:00

  • Arrangement of glycan chains in the sacculus of Escherichia coli.

    abstract::A novel of Escherichia coli endopeptidase was used for a selective partial hydrolysis of the peptide bridges which interlink the glycan chains in E. coli sacculi. The loosening of the murein network revealed, in the electron microscope, a preferential orientation of the glycan chains, more or less perpendicular to the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.2.723-729.1978

    authors: Verwer RW,Nanninga N,Keck W,Schwarz U

    更新日期:1978-11-01 00:00:00

  • A transposase-independent mechanism gives rise to precise excision of IS256 from insertion sites in Staphylococcus epidermidis.

    abstract::The mobile element IS256 causes phase variation of biofilm formation in Staphylococcus epidermidis by insertion and precise excision from the icaADBC operon. Precise excision, i.e., removal of the target site duplications (TSDs) and restoration of the original DNA sequence, occurs rarely but independently of functiona...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01290-07

    authors: Hennig S,Ziebuhr W

    更新日期:2008-02-01 00:00:00

  • Effect of degradative plasmid CAM-OCT on responses of Pseudomonas bacteria to UV light.

    abstract::The effect of plasmid CAM-OCT on responses to UV irradiation was compared in Pseudomonas aeruginosa, in Pseudomonas putida, and in Pseudomonas putida mutants carrying mutations in UV response genes. CAM-OCT substantially increased both survival and mutagenesis in the two species. P. aeruginosa strains without CAM-OCT ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.2.975-982.1989

    authors: McBeth DL

    更新日期:1989-02-01 00:00:00

  • Major proteins of the Escherichia coli outer cell envelope membrane as bacteriophage receptors.

    abstract::Three Escherichia coli phages, TuIa, TuIb, and TuII, were isolated from local sewage. We present evidence that they use the major outer membrane proteins Ia, Ib, and II, respectively, as receptors. In all cases the proteins, under the experimental conditions used, required lipopolysaccharide to exhibit their receptor ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.131.3.821-829.1977

    authors: Datta DB,Arden B,Henning U

    更新日期:1977-09-01 00:00:00

  • A self-splicing group I intron in DNA polymerase genes of T7-like bacteriophages.

    abstract::Group I introns are inserted into genes of a wide variety of bacteriophages of gram-positive bacteria. However, among the phages of enteric and other gram-negative proteobacteria, introns have been encountered only in phage T4 and several of its close relatives. Here we report the insertion of a self-splicing group I ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.23.8153-8155.2004

    authors: Bonocora RP,Shub DA

    更新日期:2004-12-01 00:00:00

  • Ribosomes, polyribosomes, and deoxyribonucleic acid from thermophilic mesophilic, and psychrophilic clostridia.

    abstract::Analysis of deoxyribonucleic acid (DNA) from four species of Clostridium, including two thermophiles, a mesophile, and a psychrophile, revealed no obvious relationship between growth temperature and DNA base composition. The melting temperatures (T(m)) of the DNA from the four species varied no more among the thermoph...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.1.252-262.1973

    authors: Irwin CC,Akagi JM,Himes RH

    更新日期:1973-01-01 00:00:00

  • The essential β-barrel assembly machinery complex components BamD and BamA are required for autotransporter biogenesis.

    abstract::Autotransporter biogenesis is dependent upon BamA, a central component of the β-barrel assembly machinery (BAM) complex. In this report, we detail the role of the other BAM components (BamB-E). We identify the importance of BamD in autotransporter biogenesis and show that BamB, BamC, and BamE are not required. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00192-11

    authors: Rossiter AE,Leyton DL,Tveen-Jensen K,Browning DF,Sevastsyanovich Y,Knowles TJ,Nichols KB,Cunningham AF,Overduin M,Schembri MA,Henderson IR

    更新日期:2011-08-01 00:00:00

  • Myxococcus xanthus sasN encodes a regulator that prevents developmental gene expression during growth.

    abstract::Myxococcus xanthus multicellular fruiting body development is initiated by nutrient limitation at high cell density. Five clustered point mutations (sasB5, -14, -15, -16, and -17) can bypass the starvation and high-cell-density requirements for expression of the 4521 developmental reporter gene. These mutants express ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Xu D,Yang C,Kaplan HB

    更新日期:1998-12-01 00:00:00

  • Control of transcription of gal repressor and isorepressor genes in Escherichia coli.

    abstract::Two regulatory proteins, Gal repressor and isorepressor, control the expression of the gal and mgl operons in Escherichia coli. The transcription start sites for galR and galS, the genes for the repressor and isorepressor, were determined by primer extension of in vivo transcripts. Study of the promoter-lacZ gene fusi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.1.251-258.1993

    authors: Weickert MJ,Adhya S

    更新日期:1993-01-01 00:00:00

  • Aerobic metabolism of Streptococcus agalactiae.

    abstract::Streptococcus agalactiae cultures possess an aerobic pathway for glucose oxidation that is strongly inhibited by cyanide. The products of glucose oxidation by aerobically grown cells of S. agalactiae 50 are lactic and acetic acids, acetylmethylcarbinol, and carbon dioxide. Glucose degradation products by aerobically g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.1.184-191.1967

    authors: Mickelson MN

    更新日期:1967-07-01 00:00:00

  • Molecular characterization of the alpha-glucosidase gene (malA) from the hyperthermophilic archaeon Sulfolobus solfataricus.

    abstract::Acidic hot springs are colonized by a diversity of hyperthermophilic organisms requiring extremes of temperature and pH for growth. To clarify how carbohydrates are consumed in such locations, the structural gene (malA) encoding the major soluble alpha-glucosidase (maltase) and flanking sequences from Sulfolobus solfa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.5.1287-1295.1998

    authors: Rolfsmeier M,Haseltine C,Bini E,Clark A,Blum P

    更新日期:1998-03-01 00:00:00

  • Germination and outgrowth of single spores of Saccharomyces cerevisiae viewed by scanning electron and phase-contrast microscopy.

    abstract::Single spores of Saccharomyces cerevisiae were examined during germination and outgrowth by scanning electron and phase-contrast microscopy. Also determined were changes in cell weight and light absorbance, trehalose utilization, and synthesis of protein and KOH-soluble carbohydrates. These studies reveal that develop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.3.1232-1238.1972

    authors: Rousseau P,Halvorson HO,Bulla LA Jr,St Julian G

    更新日期:1972-03-01 00:00:00

  • Identification of a phosphoenolpyruvate:fructose 1-phosphotransferase system in Azospirillum brasilense.

    abstract::An inducible phosphoenolpyruvate:fructose phosphotransferase system has been detected in Azospirillum brasilense, which requires a minimum of two components of the crude extracts for activity: (i) a soluble fraction (enzyme I) and (ii) a membrane fraction (enzyme II). The uninduced cells neither show any uptake of fru...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.3.1204-1206.1984

    authors: Gupta KD,Ghosh S

    更新日期:1984-12-01 00:00:00

  • TOL plasmid in Pseudomonas aeruginosa PAO: thermosensitivity of self-maintenance and inhibition of host cell growth.

    abstract::The TOL plasmid originally isolated in Pseudomonas putida (arvilla) mt-2 was transmissible to strains of the fluorescens group of Pseudomonas, i.e., P. putida, P. fluorescens, and P. aeruginosa, except for a strain of P. aeruginosa, strain PAO. The same strain, however, could accept the plasmid when its restriction an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.2.527-535.1978

    authors: Nakazawa T

    更新日期:1978-02-01 00:00:00

  • FlhD/FlhC is a regulator of anaerobic respiration and the Entner-Doudoroff pathway through induction of the methyl-accepting chemotaxis protein Aer.

    abstract::The regulation by two transcriptional activators of flagellar expression (FlhD and FlhC) and the chemotaxis methyl-accepting protein Aer was studied with glass slide DNA microarrays. An flhD::Kan insertion and an aer deletion were independently introduced into two Escherichia coli K-12 strains, and the effects upon ge...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.2.534-543.2003

    authors: Prüss BM,Campbell JW,Van Dyk TK,Zhu C,Kogan Y,Matsumura P

    更新日期:2003-01-01 00:00:00

  • Isolation and characterization of ribosomes and translation initiation factors from the gram-positive soil bacterium Streptomyces lividans.

    abstract::A primer extension inhibition (toeprint) assay was developed using ribosomes and ribosomal subunits from Streptomyces lividans. This assay allowed the study of ribosome binding to streptomycete leaderless and leadered mRNA. Purified 30S subunits were unable to form a ternary complex on aph leaderless mRNA, whereas 70S...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.20.6864-6875.2004

    authors: Day JM,Janssen GR

    更新日期:2004-10-01 00:00:00

  • Construction and characterization of an Escherichia coli strain with a uncI mutation.

    abstract::A strain of Escherichia coli with a mutation in the promoter proximal gene ( uncI ) of the unc operon has been constructed by using a new gene replacement method. The mutation is a deletion of a defined sequence of 196 base pairs. It was constructed by homologous integration and segregation of a ColE1-derived recombin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.3.820-825.1984

    authors: Gay NJ

    更新日期:1984-06-01 00:00:00

  • A LysR-type regulator, CidR, is required for induction of the Staphylococcus aureus cidABC operon.

    abstract::The Staphylococcus aureus cidABC and lrgAB operons have been shown to regulate murein hydrolase activity and affect antibiotic tolerance. The cid operon enhances murein hydrolase activity and antibiotic sensitivity, whereas the lrg operon inhibits these processes. Based on these findings and the structural similaritie...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.17.5893-5900.2005

    authors: Yang SJ,Rice KC,Brown RJ,Patton TG,Liou LE,Park YH,Bayles KW

    更新日期:2005-09-01 00:00:00

  • Effect of uncouplers on "downhill" beta-galactoside transport in energy-depleted cells of Escherichia coli.

    abstract::Galactoside permease-containing cells of Escherichia coli can be depleted of energy reserves so that the "downhill" cellular hydrolysis of o-nitrophenyl-beta-d-galactopyranoside (ONPG) no longer takes place. Treatment of such energy-depleted cells with proton-conducting agents such as carbonylcyanide m-chlorophenylhyd...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.1.187-195.1975

    authors: Cecchini G,Koch AL

    更新日期:1975-07-01 00:00:00

  • Two Anabaena sp. strain PCC 7120 DNA-binding factors interact with vegetative cell- and heterocyst-specific genes.

    abstract::The DNA-binding factor BifA (previously called VF1) binds upstream of the developmentally regulated site-specific recombinase gene xisA in the cyanobacterium Anabaena sp. strain PCC 7120. Besides binding xisA, BifA also binds the glnA, rbcL, and nifH promoter regions. DNase I footprint analysis of BifA binding to glnA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.5.1214-1223.1994

    authors: Ramasubramanian TS,Wei TF,Golden JW

    更新日期:1994-03-01 00:00:00

  • Mutations in the Escherichia coli dnaG gene suggest coupling between DNA replication and chromosome partitioning.

    abstract::Eleven conditional lethal dnaG(Ts) mutations were located by chemical cleavage of heteroduplexes formed between polymerase chain reaction-amplified DNAs from wild-type and mutant dnaG genes. This entailed end labeling one DNA strand of the heteroduplex, chemically modifying the strands with hydroxylamine or osmium tet...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.3.1268-1278.1991

    authors: Grompe M,Versalovic J,Koeuth T,Lupski JR

    更新日期:1991-02-01 00:00:00

  • Ultrastructural alterations associated with the growth of resistant Pseudomonas aeruginosa in the presence of benzalkonium chloride.

    abstract::Cells of Pseudomonas aeruginosa resistant to benzalkonium chloride (BC) underwent unique ultrastructural reorganizations when they were grown in the presence of 1 mg of BC/ml. The resistant cells usually contained a single, centrally positioned pseudovacuole. The pseudovacuole was surrounded by a diffuse substance tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.1.409-416.1973

    authors: Hoffmann HP,Geftic SG,Gelzer J,Heymann H,Adair FW

    更新日期:1973-01-01 00:00:00

  • Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells.

    abstract::Gilden, R. V. (Wistar Institute, Philadelphia, Pa.), and R. I. Carp. Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells. J. Bacteriol. 91:1295-1297. 1966.-Synthesis of the simian virus 40 (SV40) T antigen in primary African green monkey kidney cells was abolis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.3.1295-1297.1966

    authors: Gilden RV,Carp RI

    更新日期:1966-03-01 00:00:00