A self-splicing group I intron in DNA polymerase genes of T7-like bacteriophages.

Abstract:

:Group I introns are inserted into genes of a wide variety of bacteriophages of gram-positive bacteria. However, among the phages of enteric and other gram-negative proteobacteria, introns have been encountered only in phage T4 and several of its close relatives. Here we report the insertion of a self-splicing group I intron in the coding sequence of the DNA polymerase genes of PhiI and W31, phages that are closely related to T7. The introns belong to subgroup IA2 and both contain an open reading frame, inserted into structural element P6a, encoding a protein belonging to the HNH family of homing endonucleases. The introns splice efficiently in vivo and self-splice in vitro under mild conditions of ionic strength and temperature. We conclude that there is no barrier for maintenance of group I introns in phages of proteobacteria.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bonocora RP,Shub DA

doi

10.1128/JB.186.23.8153-8155.2004

keywords:

subject

Has Abstract

pub_date

2004-12-01 00:00:00

pages

8153-5

issue

23

eissn

0021-9193

issn

1098-5530

pii

186/23/8153

journal_volume

186

pub_type

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