A transposase-independent mechanism gives rise to precise excision of IS256 from insertion sites in Staphylococcus epidermidis.

abstract：:Toxin and antitoxin (TA) gene pairs are addiction systems that are present in many microbial genomes. Sinorhizobium meliloti is an N2-fixing bacterial symbiont of alfalfa and other leguminous plants, and its genome consists of three large replicons, a circular chromosome (3.7 Mb) and the megaplasmids pSymA (1.4 Mb) an...

journal_title：Journal of bacteriology

authors： Milunovic B,diCenzo GC,Morton RA,Finan TM

更新日期：2014-02-01 00:00:00

abstract：:The level of extracellular alpha-amylase (EC 3.2.1.1) of Bacillus subtilis Marburg was increased about fivefold by introducing the amyR marker from B. natto 1212 through transformation. amyR2 of B. natto 1212 has been assumed to determine a high level of alpha-amylase of the organism. The gene acts specifically on alp...

journal_title：Journal of bacteriology

authors： Yamaguchi K,Nagata Y,Maruo B

更新日期：1974-08-01 00:00:00

abstract：:Pyridine nucleotide-dependent glucose dehydrogenase activity (GPND) is described for the first time in cell-free extracts of certain blue-green algae. When glucose is added to these crude cell extracts, nicotinamide adenine dinucleotide phosphate is reduced at twice the rate as nicotinamide adenine dinucleotide; but e...

journal_title：Journal of bacteriology

authors： Pulich WM,Van Baalen C

更新日期：1973-04-01 00:00:00

abstract：:We previously reported that the methicillin resistance gene mecA is carried by a novel type of mobile genetic element, SCCmec (staphylococcal cassette chromosome mec), in the chromosome of methicillin-resistant Staphylococcus aureus (MRSA). These elements are precisely excised from the chromosome and integrated into a...

journal_title：Journal of bacteriology

authors： Katayama Y,Takeuchi F,Ito T,Ma XX,Ui-Mizutani Y,Kobayashi I,Hiramatsu K

更新日期：2003-05-01 00:00:00

abstract：:We identified in Streptococcus mutans six new genes (rgpA through rgpF), whose disruption results in a loss of serotype-specific antigenicity, specified by the glucose side chains of rhamnose-glucose polysaccharide from the cell wall. Rhamnose and glucose content of the cell wall decreased drastically in all these dis...

journal_title：Journal of bacteriology

authors： Yamashita Y,Tsukioka Y,Tomihisa K,Nakano Y,Koga T

更新日期：1998-11-01 00:00:00

abstract：:The transfer of plasmid DNA molecules between bacterial cells is achieved by a large array of conjugative transfer proteins which assemble into both cytoplasmic and membrane-associated complexes. TrhC is a membrane-associated protein that is required for the transfer of the IncHI1 resistance plasmid R27. Homologous pr...

journal_title：Journal of bacteriology

authors： Gilmour MW,Taylor DE

更新日期：2004-03-01 00:00:00

abstract：:An obligate thermophilic strain of Bacillus stearothermophilus, strain PH24, isolated from industrial sediment by elective culture, grew readily at 55 C on phenol or on one of the isomers of cresol as the major carbon source. Intact cells grown in the presence of phenol, o-cresol, m-cresol, or p-cresol were induced to...

journal_title：Journal of bacteriology

authors： Buswell JA

更新日期：1975-12-01 00:00:00

abstract：:Current mouse models for evaluating the efficacy of live oral cholera vaccines (OCVs) have important limitations. Conventionally raised adult mice are resistant to intestinal colonization by Vibrio cholerae, but germfree mice can be colonized and have been used to study OCV immunogenicity. However, germfree animals ha...

journal_title：Journal of bacteriology

authors： Fakoya B,Sit B,Waldor MK

更新日期：2020-11-19 00:00:00

abstract：:Independently controlled, inducible, catabolic genes in Pseudomonas aeruginosa are subject to strong catabolite repression control by intermediates of the tricarboxylic acid cycle. Mutants which exhibited a pleiotropic loss of catabolite repression control of multiple pathways were isolated. The mutations mapped in th...

journal_title：Journal of bacteriology

authors： Wolff JA,MacGregor CH,Eisenberg RC,Phibbs PV Jr

更新日期：1991-08-01 00:00:00

abstract：:Plasmids for high-level expression of penicillin-binding protein 6 (PBP6) were constructed, giving rise to overproduction of PBP6 under the control of the lambda pR promoter in either the periplasmic or the cytoplasmic space. In contrast to penicillin-binding protein 5 (PBP5), the presence of high amounts of PBP6 in t...

journal_title：Journal of bacteriology

authors： van der Linden MP,de Haan L,Hoyer MA,Keck W

更新日期：1992-12-01 00:00:00

abstract：:Polyglutamate is found in various bacteria, but displays different functions depending on the species and their environment. Here, we describe a minimal polyglutamate synthesis system in Bacillus anthracis. In addition to the three genes previously described as sufficient for polyglutamate synthesis, this system inclu...

journal_title：Journal of bacteriology

authors： Candela T,Mock M,Fouet A

更新日期：2005-11-01 00:00:00

abstract：:Payne, W. J. (University of Georgia, Athens), and R. L. Todd. Flavin-linked dehydrogenation of ether glycols by cell-free extracts of a soil bacterium. J. Bacteriol. 91:1533-1536. 1966.-Cell-free extracts of bacterium TEG-5 grown on tetraethylene glycol dehydrogenated a variety of ether glycols and nonylphenoxy and se...

journal_title：Journal of bacteriology

authors： Payne WJ,Todd RL

更新日期：1966-04-01 00:00:00

abstract：:Stable, metabolically active protoplasts of Staphylococcus aureus have been prepared by the use of a staphylolytic enzyme produced by Aeromonas hydrophila. Respiratory and glycolytic rates and synthesis of nucleic acids, protein, and lipid in these protoplasts, stabilized variously in 1.1 M sucrose, 0.37 M sodium succ...

journal_title：Journal of bacteriology

authors： Coles NW,Gross R

更新日期：1973-09-01 00:00:00

abstract：:A RecA-independent chromosomal rearrangement in the upstream region of the streptolysin O (slo) gene of Streptococcus pyogenes which affects slo expression was identified. PCR analysis was used to demonstrate that this kind of rearrangement was found in several strains of different lineages. Chromosomal loci involved ...

journal_title：Journal of bacteriology

authors： Savic DJ,Ferretti JJ

更新日期：2003-03-01 00:00:00

abstract：:Aspartate transcarbamylase is synthesized during exponential growth of Bacillus subtilis and is inactivated when the cells enter the stationary phase. This work is a study of the regulation of aspartate transcarbamylase synthesis during growth and the stationary phase. Using specific immunoprecipitation of aspartate t...

journal_title：Journal of bacteriology

authors： Maurizi MR,Switzer RL

更新日期：1978-09-01 00:00:00

abstract：:Neisseria meningitidis expresses multicomponent organelles called type four pili (Tfp), which are key virulence factors required for attachment to human cells during carriage and disease. Pilin (PilE) is the main component of Tfp, and N. meningitidis isolates either have a class I pilE locus and express pilins that un...

journal_title：Journal of bacteriology

authors： Lobanovska M,Tang CM,Exley RM

更新日期：2019-09-20 00:00:00

abstract：:Genetic studies have shown that the 53-kDa (Exo53) and 49-kDa (ExoS) forms of exoenzyme S of Pseudomonas aeruginosa are encoded by separate genes, termed exoT and exoS, respectively. Although ExoS and Exo53 possess 76% primary amino acid homology, Exo53 has been shown to express ADP-ribosyltransferase activity at abou...

journal_title：Journal of bacteriology

authors： Liu S,Yahr TL,Frank DW,Barbieri JT

更新日期：1997-03-01 00:00:00

abstract：:Conjugative transfer and replacement of hundreds of kilobases of a bacterial chromosome can occur in vitro, but replacements in nature are either an order of magnitude smaller or involve the movement of mobile genetic elements. We discovered that two lineages of Staphylococcus aureus, including a pandemic methicillin-...

journal_title：Journal of bacteriology

authors： Robinson DA,Enright MC

更新日期：2004-02-01 00:00:00

abstract：:The occurrence of the novel regulatory nucleotide bis(3',5')-cyclic diguanylic acid (c-di-GMP) and its relation to cellulose biogenesis in the plant pathogen Agrobacterium tumefaciens was studied. c-di-GMP was detected in acid extracts of 32P-labeled cells grown in various media, and an enzyme responsible for its form...

journal_title：Journal of bacteriology

authors： Amikam D,Benziman M

更新日期：1989-12-01 00:00:00

abstract：:Myxobacterial hemagglutinin (MBHA) is a major developmentally induced protein that accumulates during the period of cellular aggregation in the bacterium Myxococcus xanthus. It has been shown that this lectin is targeted to the cell surface and periplasmic space of developmental cells, suggesting that it may play a ro...

journal_title：Journal of bacteriology

authors： Romeo JM,Zusman DR

更新日期：1987-08-01 00:00:00

abstract：:Site-directed mutagenesis has been used to identify a number of charged residues essential for the transport activity of the PheP protein. These residues are highly conserved in the cluster of amino acid transporters. However, some other conserved residues and a number of aromatic residues have been shown not to be es...

journal_title：Journal of bacteriology

authors： Pi J,Wookey PJ,Pittard AJ

更新日期：1993-11-01 00:00:00

abstract：:We have isolated and determined the nucleotide sequence of a 1,626-base-pair fragment from R-plasmid R483 which encodes a trimethoprim-resistant dihydrofolate reductase. Analysis of the nucleotide sequence of this fragment revealed the presence of two open reading frames, each sufficient to encode polypeptides of appr...

journal_title：Journal of bacteriology

authors： Simonsen CC,Chen EY,Levinson AD

更新日期：1983-09-01 00:00:00

abstract：:Adler, H. E. (University of California, Davis), M. Shifrine, and Herrad Ortmayer. Mycoplasma inocuum sp. n., a saprophyte from chickens. J. Bacteriol. 82:239-240. 1961.-A new species of Mycoplasma was isolated from the infraorbital sinuses of chickens. The organism, a saprophyte, differs from all known mycoplasmas of ...

journal_title：Journal of bacteriology

authors： ADLER HE,SHIFRINE M,ORTMAYER H

更新日期：1961-08-01 00:00:00

abstract：:The acetohydroxy acid synthase III isozyme, which catalyzes the first common step in the biosynthesis of isoleucine, leucine, and valine in Escherichia coli K-12, is composed of two subunits, the ilvI and ilvH gene products. A missense mutation in ilvH (ilvH612), which reduced the sensitivity of the enzyme to the end ...

journal_title：Journal of bacteriology

authors： Ricca E,Limauro D,Lago CT,de Felice M

更新日期：1988-11-01 00:00:00

abstract：:The gene for beta-amylase was isolated from Bacillus polymyxa by molecular cloning in B. subtilis. B. subtilis cells containing this gene express and secrete an amylase which resembles the B. polymyxa beta-amylase and barley beta-amylase in terms of the products it generates during carbohydrate hydrolysis. Starch hydr...

journal_title：Journal of bacteriology

authors： Friedberg F,Rhodes C

更新日期：1986-03-01 00:00:00

abstract：:The Hly region from the chromosome of Vibrio cholerae El Tor strain RV79(Hly-) and the nonhemolytic classical strain 569B were cloned into plasmid vector pBR322. Escherichia coli K-12 transformants possessing these recombinant plasmids were nonhemolytic and were detected with a 32P-labeled hly-specific DNA probe. Rest...

journal_title：Journal of bacteriology

authors： Goldberg SL,Murphy JR

更新日期：1985-04-01 00:00:00

abstract：:High-density tiling microarray and RNA sequencing technologies were used to analyze the transcriptome of the periodontopathogenic bacterium Porphyromonas gingivalis. The compiled P. gingivalis transcriptome profiles were based on total RNA samples isolated from three different laboratory culturing conditions, and the ...

journal_title：Journal of bacteriology

authors： Høvik H,Yu WH,Olsen I,Chen T

更新日期：2012-01-01 00:00:00

abstract：:The genes that encode the two subunits of Bacillus subtilis phenylalanyl-tRNA synthetase were cloned from alpha lambda library of chromosomal B. subtilis DNA by specific complementation of a thermosensitive Escherichia coli pheS mutation. Both genes (we named them pheS and pheT, analogous to the corresponding genes of...

journal_title：Journal of bacteriology

authors： Brakhage AA,Putzer H,Shazand K,Röschenthaler RJ,Grunberg-Manago M

更新日期：1989-02-01 00:00:00

abstract：:Two replicons were isolated independently from different IncHI1 plasmids. One was isolated from R27, and a second was isolated from pIP522. We demonstrate, by DNA-DNA hybridization experiments, that these maintenance regions are different and that they are specific to, and carried by, all IncHI1 plasmids tested. In vi...

journal_title：Journal of bacteriology

authors： Gabant P,Newnham P,Taylor D,Couturier M

更新日期：1993-12-01 00:00:00

abstract：:The crtEF, bchCA, and puf operons of the facultative phototrophic bacterium Rhodobacter capsulatus encode gene products that are necessary for the formation of various components of the photosynthetic apparatus. The crtEF operon encodes two enzymes involved in the biosynthesis of carotenoids, the bchCA operon codes fo...

journal_title：Journal of bacteriology

authors： Wellington CL,Beatty JT

更新日期：1991-02-01 00:00:00