Novel genomic rearrangement that affects expression of the Streptococcus pyogenes streptolysin O (slo) gene.

Abstract:

:A RecA-independent chromosomal rearrangement in the upstream region of the streptolysin O (slo) gene of Streptococcus pyogenes which affects slo expression was identified. PCR analysis was used to demonstrate that this kind of rearrangement was found in several strains of different lineages. Chromosomal loci involved in the recombination were found to be 746 kb apart on the 1.85-Mb-long chromosome. The primary structure of the splicing region, the reproducibility of the rearrangement, and the fact that reconstructed recombinant molecules fused to erm and lacZ reporter genes affected their expression indicate that this event is not accidental but may play a role in the expression of the slo gene. In addition, the product of the recombining DNAs, including the splicing site, does not follow any example of a known recombination mechanism. The implications of this rearrangement for slo expression are discussed.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Savic DJ,Ferretti JJ

doi

10.1128/jb.185.6.1857-1869.2003

keywords:

subject

Has Abstract

pub_date

2003-03-01 00:00:00

pages

1857-69

issue

6

eissn

0021-9193

issn

1098-5530

journal_volume

185

pub_type

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