Abstract:
:A RecA-independent chromosomal rearrangement in the upstream region of the streptolysin O (slo) gene of Streptococcus pyogenes which affects slo expression was identified. PCR analysis was used to demonstrate that this kind of rearrangement was found in several strains of different lineages. Chromosomal loci involved in the recombination were found to be 746 kb apart on the 1.85-Mb-long chromosome. The primary structure of the splicing region, the reproducibility of the rearrangement, and the fact that reconstructed recombinant molecules fused to erm and lacZ reporter genes affected their expression indicate that this event is not accidental but may play a role in the expression of the slo gene. In addition, the product of the recombining DNAs, including the splicing site, does not follow any example of a known recombination mechanism. The implications of this rearrangement for slo expression are discussed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Savic DJ,Ferretti JJdoi
10.1128/jb.185.6.1857-1869.2003keywords:
subject
Has Abstractpub_date
2003-03-01 00:00:00pages
1857-69issue
6eissn
0021-9193issn
1098-5530journal_volume
185pub_type
杂志文章abstract::Cell survival, deoxyribonucleic acid (DNA) degradation, and the repair of DNA single-strand breaks were measured for Escherichia coli K-12 pol+, polA1, polC1026(ts), and polA1 polC1026(ts) cells after 137Cs gamma irradiation. The results indicate that DNA polymerase III is required for growth medium-dependent (type II...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.3.1307-1314.1976
更新日期:1976-09-01 00:00:00
abstract::Transducing frequencies of phage P22 lysates prepared from Salmonella typhimurium exponential cultures in minimal and nutrient broth media were compared. The assumption is that cells grown in a minimal medium will have one replication fork per replication unit, but cells in nutrient broth will have multiple replicatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.1.168-176.1973
更新日期:1973-07-01 00:00:00
abstract::In many bacteria, the ferric uptake regulator (Fur) protein plays a central role in the regulation of iron uptake genes. Because iron figures prominently in the agriculturally important symbiosis between soybean and its nitrogen-fixing endosymbiont Bradyrhizobium japonicum, we wanted to assess the role of Fur in the i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.5.1409-1414.2004
更新日期:2004-03-01 00:00:00
abstract::Pseudomonas aeruginosa PAO1 catabolized the aromatic amines tyramine and octopamine through 4-hydroxyphenylacetic acid and 3,4-dihydroxyphenylacetic acid (HPA). meta ring cleavage was mediated by 3,4-dihydroxyphenylacetate 2,3-dioxygenase (HPADO), producing 2-hydroxy-5-carboxymethylmuconic semialdehyde (MSA). An NAD-d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.393-399.1988
更新日期:1988-01-01 00:00:00
abstract::The suf operon is composed of four genes (sufB, sufC, sufD, and sufS) and is highly conserved in the genomes of cyanobacteria. Open reading frame sll0088 in Synechocystis sp. strain PCC 6803 is located near the 5' end of the suf operon but is transcribed in the direction opposite that of the suf operon. We previously ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.4.956-967.2004
更新日期:2004-02-01 00:00:00
abstract::Optochin-resistant mutant and wild-type diaphorases were purified approximately 300-fold by a combination of batch adsorption and column chromatography with diethylaminoethyl cellulose, and were characterized with regard to their pH optima, sensitivity to optochin inhibition and heat inactivation, Michaelis constants ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.4.1029-1036.1968
更新日期:1968-10-01 00:00:00
abstract::Xylose is rarely described as a component of bacterial glycans. UDP-xylose is the nucleotide-activated form necessary for incorporation of xylose into glycans and is synthesized by the decarboxylation of UDP-glucuronic acid (UDP-GlcA). Enzymes with UDP-GlcA decarboxylase activity include those that lead to the formati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05337-11
更新日期:2011-10-01 00:00:00
abstract::A newly isolated bacterium, Pseudomonas geniculata N1, can efficiently degrade nicotine. Here we present a 4.51-Mb assembly of its genome, which is the first sequence of the P. geniculata group. The sequence contains the genes related to nicotine catabolism and may provide insights into its molecular mechanism for N-h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00625-12
更新日期:2012-07-01 00:00:00
abstract::Controlled acetolysis of yeast mannans yields mixtures of oligosaccharides with (1-->2) and (1-->3) linkages between the mannose units, whereas the less stable (1-->6) linkages of the polysaccharide backbone are cleaved. The "fingerprints," obtained by gel filtration of the oligosaccharide mixtures, can be used to dis...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.3.1175-1181.1969
更新日期:1969-12-01 00:00:00
abstract::Studies on the active product of pepsin digestion of rabbit immunoglobulin G (IgG), F(ab')(2) produced from antiserum against Neurospora glutamate dehydrogenase, showed it to behave in the same way, in both antienzyme and precipitation experiments, as homologous IgG. It is proposed that the inhibition of glutamate deh...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.4.958-962.1967
更新日期:1967-10-01 00:00:00
abstract::Investigation of the chromosomal region downstream of the lacZ gene from Lactobacillus delbrueckii subsp. bulgaricus revealed the presence of a gene (prtB) encoding a proteinase of 1,946 residues with a predicted molecular mass of 212 kDa. The deduced amino acid sequence showed that PrtB proteinase displays significan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.11.3059-3065.1996
更新日期:1996-06-01 00:00:00
abstract::In this study, the H303A mutant form of the cbb(3) oxidase (H303A oxidase), which has the H303A mutation in its catalytic subunit (CcoN), was purified from Rhodobacter sphaeroides. The H303A oxidase showed the same catalytic activity as did the wild-type form of the oxidase (WT oxidase). The heme contents of the mutan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00443-07
更新日期:2007-08-01 00:00:00
abstract::ibeA is a virulence factor found in some extraintestinal pathogenic Escherichia coli (ExPEC) strains from the B2 phylogenetic group and particularly in newborn meningitic and avian pathogenic strains. It was shown to be involved in the invasion process of the newborn meningitic strain RS218. In a previous work, we sho...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00089-12
更新日期:2012-09-01 00:00:00
abstract::Neonatal bacterial meningitis continues to be an important cause of mortality and morbidity worldwide. Escherichia coli possessing the K1 capsular polysaccharide is the most common Gram-negative pathogen causing neonatal meningitis. Here we present the complete genome sequence of neonatal meningitis-associated E. coli...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06284-11
更新日期:2011-12-01 00:00:00
abstract::Cybulska, Janina (State Institute of Hygiene, Warsaw, Poland), and J. Jeljaszewicz. Bacteriostatic activity of serum against staphylococci. J. Bacteriol. 91:953-962. 1966.-Antistaphylococcal activity of normal serum against strains exhibiting various patterns of coagulase, clumping-factor, and staphylokinase productio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.3.953-962.1966
更新日期:1966-03-01 00:00:00
abstract::Lactobacillus casei and Streptococcus faecalis accumulated labeled folic acid and metabolized this compound to poly-gamma-glutamates of chain lengths of up to 11 and 5, respectively. Octa- and nonaglutamates predominated in L. casei, and tetraglutamates predominated in S. faecalis. The most effective monoglutamate sub...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.316-325.1983
更新日期:1983-01-01 00:00:00
abstract::Selection for chlorate resistance yields mol (formerly chl) mutants with defects in molybdenum cofactor synthesis. Complementation and genetic mapping analyses indicated that the Klebsiella pneumoniae mol genes are functionally homologous to those of Escherichia coli and occupy analogous genetic map positions. Hypoxan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.19.6298-6302.1992
更新日期:1992-10-01 00:00:00
abstract::Picolinic acid (PA), a typical C-2-carboxylated pyridine derivative, is a metabolite of l-tryptophan and many other aromatic compounds in mammalian and microbial cells. Microorganisms can degrade and utilize PA for growth. However, the precise mechanism of PA metabolism remains unknown. Alcaligenes faecalis strain JQ1...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00665-18
更新日期:2019-03-13 00:00:00
abstract::The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique protein...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.23.8172-8180.2005
更新日期:2005-12-01 00:00:00
abstract::The cheA locus of Escherichia coli encodes two similar proteins, CheAL (654 amino acids) and CheAS (557 amino acids), which are made by initiating translation from different in-frame start sites [start(L) and start(S)]. CheAL plays an essential role in chemotactic signaling. It autophosphorylates at a histidine residu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.10.2713-2720.1995
更新日期:1995-05-01 00:00:00
abstract::Cells colicinogenic for the colicin plasmids E1 or E2 (Col E1 and Col E2, respectively) were selected for a loss of colicin production after infection with bacteriophage Mu. Extrachromosomal deoxyribonucleic acid that was larger than the original colicin plasmids was found in such cells. A small insertion mutant in Co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.2.469-477.1974
更新日期:1974-08-01 00:00:00
abstract::A number of gram-negative bacteria have a quorum-sensing system and produce N-acyl-l-homoserine lactone (AHL) that they use them as a quorum-sensing signal molecule. Pantoea ananatis is reported as a common colonist of wheat heads at ripening and causes center rot of onion. In this study, we demonstrated that P. anana...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01054-07
更新日期:2007-11-01 00:00:00
abstract::Antiserum to Aeromonas hydrophila A6 cell envelopes was shown in a previous study (C. Y. F. Wong, G. Mayrhofer, M. W. Heuzenroeder, H. M. Atkinson, D. M. Quinn, and R. L. P. Flower, FEMS Immunol. Med. Microbiol. 15:233-241, 1996) to protect mice against lethal infection by this organism. In this study, colony blot ana...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.11.3397-3403.1997
更新日期:1997-06-01 00:00:00
abstract::The YycFG two-component system is the only signal transduction system in Bacillus subtilis known to be essential for cell viability. This system is highly conserved in low-G+C gram-positive bacteria, regulating important processes such as cell wall homeostasis, cell membrane integrity, and cell division. Four other ge...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01936-06
更新日期:2007-04-01 00:00:00
abstract::NADPH is an intermediate in the oxidation of organic compounds coupled to Fe(III) reduction in Geobacter species, but Fe(III) reduction with NADPH as the electron donor has not been studied in these organisms. Crude extracts of Geobacter sulfurreducens catalyzed the NADPH-dependent reduction of Fe(III)-nitrilotriaceti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.15.4468-4476.2001
更新日期:2001-08-01 00:00:00
abstract::Germination and outgrowth of ascospores of Saccharomyces cerevisiae 4579 require both methionine and adenine, whereas leucine is only required for outgrowth. The methionine requirement may be satisfied by S-adenosylmethionine, but this sulfonium compound will not substitute for adenine. Between 30 and 70 min of protei...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.1.63-68.1977
更新日期:1977-07-01 00:00:00
abstract::The plasmid-encoded citrate determinant of the Lactococcus lactis subsp. lactis var. diacetylactis NCDO176 was cloned and functionally expressed in a Cit- Escherichia coli K-12 strain. From deletion derivative analysis, a 3.4-kilobase region was identified which encodes the ability to transport citrate. Analysis of pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.5789-5794.1990
更新日期:1990-10-01 00:00:00
abstract::LamB-LacZ fusion proteins have classically been used in studies of the general secretion pathway of Escherichia coli. Here we describe how increasing signal sequence hydrophobicity routes LamB-LacZ Hyb42-1 to the signal recognition particle (SRP) pathway. Secretion of this hydrophobic fusion variant (H*LamB-LacZ) was ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.19.5697-5705.2003
更新日期:2003-10-01 00:00:00
abstract::Decreased intracellular levels of FtsH, a membrane-bound ATPase, led to retardation of growth and protein export, as well as to an abnormal translocation of alkaline phosphatase that had been attached to a cytoplasmic domain of a multispanning membrane protein, SecY. The last phenotype is designated Std (stop transfer...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.4.1141-1145.1996
更新日期:1996-02-01 00:00:00
abstract::The type IV pili of plasmid R64 belonging to the type IVB group are required only for liquid mating. They consist of the major and minor components PilS pilin and PilV adhesin, respectively. PilS pilin is first synthesized as a 22-kDa prepilin from the pilS gene and is then processed to a 19-kDa mature pilin by PilU p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01204-07
更新日期:2008-02-01 00:00:00