Cloning and characterization of the beta-amylase gene from Bacillus polymyxa.

abstract：:A thermosensitive episome bearing the transposon Tn10, F(Ts)::Tn10 Lac+, has been successfully transferred from Escherichia coli to several wild strains of the enterobacteria Erwinia carotovora subsp. chrysanthemi, which are pathogenic on Saintpaulia ionantha. In one of these strains, all of the characters controlled ...

journal_title：Journal of bacteriology

authors： Kotoujansky A,Lemattre M,Boistard P

更新日期：1982-04-01 00:00:00

abstract：:Succinyl-coenzyme A synthetase of Escherichia coli has an alpha 2 beta 2 subunit structure. By measuring reconstituted enzyme activity present after addition of purified alpha or beta subunits to cell extracts followed by refolding, we have shown that extracts contain no significant excess of either subunit species. T...

journal_title：Journal of bacteriology

authors： Wolodko WT,Brownie ER,Bridger WA

更新日期：1980-07-01 00:00:00

abstract：:The ability to respond to ambient pH is critical to the growth and virulence of the fungal pathogen Candida albicans. This response entails the differential expression of several genes affecting morphogenesis. To investigate the mechanism of pH-dependent gene expression, the C. albicans homolog of pacC, designated PRR...

journal_title：Journal of bacteriology

authors： Ramon AM,Porta A,Fonzi WA

更新日期：1999-12-01 00:00:00

abstract：:The reactivation of membranes from Gaffkya homari for the synthesis of sodium dodecyl sulfate-insoluble peptidoglycan (SDS-insoluble PG) was achieved by successive cycles of freeze-thawing (- 196 versus 25 degrees C). The presence of G. homari walls during this process affected the synthesis of both SDS-soluble (nasce...

journal_title：Journal of bacteriology

authors： Kalomiris E,Bardin C,Neuhaus FC

更新日期：1982-05-01 00:00:00

abstract：:A human isolate of Salmonella enteritidis which displayed strong pellicle formation during static broth culture and mannose-sensitive hemagglutination produced fimbriae which were morphologically indistinguishable from type 1 fimbriae of members of the family Enterobacteriaceae. Fimbrin was purified to homogeneity, an...

journal_title：Journal of bacteriology

authors： Feutrier J,Kay WW,Trust TJ

更新日期：1986-10-01 00:00:00

abstract：:The role of oxygen in the transcriptional regulation of the PN promoter that controls the bzd operon involved in the anaerobic catabolism of benzoate in the denitrifying Azoarcus sp. strain CIB has been investigated. In vivo experiments using PN::lacZ translational fusions, in both Azoarcus sp. strain CIB and Escheric...

journal_title：Journal of bacteriology

authors： Durante-Rodríguez G,Zamarro MT,García JL,Díaz E,Carmona M

更新日期：2006-04-01 00:00:00

abstract：:Total synthesis of acetate from CO2 by Clostridium acidiurici during fermentations of hypoxanthine has been shown to involve synthesis of glycine from methylenetetrahydrofolate, CO2, and NH3. The glycine is converted to serine by the addition of methylenetetrahydrofolate, and the resulting serine is converted to pyruv...

journal_title：Journal of bacteriology

authors： Waber LJ,Wood HG

更新日期：1979-11-01 00:00:00

abstract：:cat-86 is a promoter-deficient plasmid gene that encodes chloramphenicol acetyltransferase (CAT). Insertion of a promoter at a site 144 base pairs 5' to the cat-86 coding sequence activates transcription of the gene and allows cat-86 to specify chloramphenicol-inducible CAT activity in Bacillus subtilis. Induction of ...

journal_title：Journal of bacteriology

authors： Ambulos NP Jr,Duvall EJ,Lovett PS

更新日期：1987-03-01 00:00:00

abstract：:In gram-negative bacteria, a pathway for aerobic degradation of phenylacetic acid (PAA) that proceeds via phenylacetyl-coenzyme A (CoA) and hydrolytic ring fission plays a central role in the degradation of a range of aromatic compounds. In contrast, the PAA pathway and its role are not well characterized in gram-posi...

journal_title：Journal of bacteriology

authors： Navarro-Llorens JM,Patrauchan MA,Stewart GR,Davies JE,Eltis LD,Mohn WW

更新日期：2005-07-01 00:00:00

abstract：:During batch growth of Alcaligenes eutrophus on benzoate-acetate mixtures, benzoate was the preferred substrate, with acetate consumption being delayed until the rate of benzoate consumption had diminished. This effect was attributed to a transcriptional control of the synthesis of acetyl coenzyme A (acetyl-CoA) synth...

journal_title：Journal of bacteriology

authors： Ampe F,Lindley ND

更新日期：1995-10-01 00:00:00

abstract：:Sequence flanking the bfpA locus on the enteroadherent factor plasmid of the enteropathogenic Escherichia coli (EPEC) strain B171-8 (O111:NM) was obtained to identify genes that might be required for bundle-forming pilus (BFP) biosynthesis. Deletion experiments led to the identification of a contiguous cluster of at l...

journal_title：Journal of bacteriology

authors： Sohel I,Puente JL,Ramer SW,Bieber D,Wu CY,Schoolnik GK

更新日期：1996-05-01 00:00:00

abstract：:Secretion vectors based on the genes from Bacillus amyloliquefaciens P for alkaline protease (aprBamP) and neutral protease (nprBamP) were constructed. With both aprBamP and nprBamP, a unique restriction site was introduced 3' of the predicted signal coding region by using the technique of oligonucleotide-directed mut...

journal_title：Journal of bacteriology

authors： Vasantha N,Thompson LD

更新日期：1986-03-01 00:00:00

abstract：:Bifidobacterium breve is an actinobacterium frequently isolated from colonic microbiota of breastfeeding babies. Here, we report the complete and annotated genome sequence of a B. breve strain isolated from human milk, B. breve CECT 7263. The genome sequence will provide new insights into the biology of this potential...

journal_title：Journal of bacteriology

authors： Jiménez E,Villar-Tajadura MA,Marín M,Fontecha J,Requena T,Arroyo R,Fernández L,Rodríguez JM

更新日期：2012-07-01 00:00:00

abstract：:Site-directed mutagenesis has been used to identify a number of charged residues essential for the transport activity of the PheP protein. These residues are highly conserved in the cluster of amino acid transporters. However, some other conserved residues and a number of aromatic residues have been shown not to be es...

journal_title：Journal of bacteriology

authors： Pi J,Wookey PJ,Pittard AJ

更新日期：1993-11-01 00:00:00

abstract：:The complex flagella of Rhizobium meliloti 2011 and MVII-1 were analyzed with regard to serology, fine structure, subunits, and amino acid composition. The serological identities of flagellar filaments of the two strains were demonstrated by double immunodiffusion with antiflagellin antiserum. The filaments had a diam...

journal_title：Journal of bacteriology

authors： Krupski G,Götz R,Ober K,Pleier E,Schmitt R

更新日期：1985-04-01 00:00:00

abstract：:Acidithiobacillus caldus is an extremely acidophilic, moderately thermophilic, chemolithoautotrophic gammaproteobacterium that derives energy from the oxidation of sulfur and reduced inorganic sulfur compounds. Here we present the draft genome sequence of Acidithiobacillus caldus ATCC 51756 (the type strain of the spe...

journal_title：Journal of bacteriology

authors： Valdes J,Quatrini R,Hallberg K,Dopson M,Valenzuela PD,Holmes DS

更新日期：2009-09-01 00:00:00

abstract：:The yfdXWUVE operon appears to encode proteins that enhance the ability of Escherichia coli MG1655 to survive under acidic conditions. Although the molecular mechanisms underlying this phenotypic behavior remain to be elucidated, findings from structural genomic studies have shown that the structure of YfdW, the prote...

journal_title：Journal of bacteriology

authors： Toyota CG,Berthold CL,Gruez A,Jónsson S,Lindqvist Y,Cambillau C,Richards NG

更新日期：2008-04-01 00:00:00

abstract：:Entry into intestinal epithelial cells is an essential step in the pathogenesis of Salmonella infections. Our laboratory has previously identified a genetic locus, inv, that is necessary for efficient entry of Salmonella typhimurium into cultured epithelial cells. We have carried out a molecular and functional analysi...

journal_title：Journal of bacteriology

authors： Eichelberg K,Ginocchio CC,Galán JE

更新日期：1994-08-01 00:00:00

abstract：:Daniel, John W. (University of Wisconsin, Madison), and Karlee Babcock. Methionine metabolism of the myxomycete Physarum polycephalum. J. Bacteriol. 92:1028-1035. 1966.-Previous studies have shown that Physarum polycephalum requires exogenous methionine for growth, but not cysteine, folic acid, or vitamin B(12). Methi...

journal_title：Journal of bacteriology

authors： Daniel JW,Babcock K

更新日期：1966-10-01 00:00:00

abstract：:Mutants for 9 of the 10 steps in histidine biosynthesis have been isolated and identified by enzyme assay. Each locus has been mapped in relation to the aro cluster and to other histidine loci by deoxyribonucleic acid-mediated transformation. The genes which code for enzymes 3, 6, and 8 of the pathway are linked to th...

journal_title：Journal of bacteriology

authors： Chapman LF,Nester EW

更新日期：1969-03-01 00:00:00

abstract：:The virA and virG gene products are required for the regulation of the vir regulon on the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens. VirA is a membrane-associated protein which is homologous to the sensor molecules of other two-component regulatory systems. We overproduced truncated VirA proteins in Esc...

journal_title：Journal of bacteriology

authors： Jin S,Roitsch T,Ankenbauer RG,Gordon MP,Nester EW

更新日期：1990-02-01 00:00:00

abstract：:Helicobacter pylori urease requires nickel ions in the enzyme active site for catalytic activity. Nickel ions must, therefore, be actively acquired by the bacterium. NixA (high-affinity nickel transport protein)-deficient mutants of H. pylori retain significant urease activity, suggesting the presence of alternate nic...

journal_title：Journal of bacteriology

authors： Hendricks JK,Mobley HL

更新日期：1997-09-01 00:00:00

abstract：:A strain of Escherichia coli which was derived from a gentamicin-resistant clinical isolate was found to be cross-resistant to neomycin and streptomycin. The molecular nature of the genetic defect was found to be an insertion of two GC base pairs in the uncG gene of the mutant. The insertion led to the production of a...

journal_title：Journal of bacteriology

authors： Humbert R,Altendorf K

更新日期：1989-03-01 00:00:00

abstract：:Sinorhizobium meliloti is a soil bacterium capable of invading and establishing a symbiotic relationship with alfalfa plants. This invasion process requires the synthesis, by S. meliloti, of at least one of the two symbiotically important exopolysaccharides, succinoglycan and EPS II. We have previously shown that the ...

journal_title：Journal of bacteriology

authors： Marketon MM,Glenn SA,Eberhard A,González JE

更新日期：2003-01-01 00:00:00

abstract：:The tuf gene which encodes peptide chain elongation factor Tu was found to be duplicated in nine enteric and four nonenteric gram-negative bacteria, but present only in one copy in two gram-positive genera. In two of the nonenteric gram-negative genera, Pseudomonas and Caulobacter, the duplicate tuf genes were found t...

journal_title：Journal of bacteriology

authors： Filer D,Furano AV

更新日期：1981-12-01 00:00:00

abstract：:TonB-gated transporters have beta-barrels containing an amino-terminal globular domain that occludes the interior of the barrel. Mutations in the globular domain prevent transport of ligands across the outer membrane. Surprisingly, FepA with deletions of the globular domain (amino acids 3 to 150 and 17 to 150) was pre...

journal_title：Journal of bacteriology

authors： Vakharia HL,Postle K

更新日期：2002-10-01 00:00:00

abstract：:Flac mutants insensitive to transfer inhibition by R factors. JR66a and R485 were isolated and characterized. Representative mutations were cis dominant and are therefore presumed to be at the sites of action, fisU and fisV, respectively, of the FinU and FinV transfer inhibition systems encoded by JR66a and R485. The ...

journal_title：Journal of bacteriology

authors： Gasson MJ,Willetts NS

更新日期：1977-08-01 00:00:00

abstract：:The phoB gene of Bacillus subtilis encodes an alkaline phosphatase (PhoB, formerly alkaline phosphatase III) that is expressed from separate promoters during phosphate deprivation in a PhoP-PhoR-dependent manner and at stage two of sporulation under phosphate-sufficient conditions independent of PhoP-PhoR. Isogenic st...

journal_title：Journal of bacteriology

authors： Abdel-Fattah WR,Chen Y,Eldakak A,Hulett FM

更新日期：2005-08-01 00:00:00

abstract：:Rhizobia form a symbiotic relationship with plants of the legume family to produce nitrogen-fixing root nodules under nitrogen-limiting conditions. We have examined the importance of glutathione (GSH) during free-living growth and symbiosis of Sinorhizobium meliloti. An S. meliloti mutant strain (SmgshA) which is unab...

journal_title：Journal of bacteriology

authors： Harrison J,Jamet A,Muglia CI,Van de Sype G,Aguilar OM,Puppo A,Frendo P

更新日期：2005-01-01 00:00:00

abstract：:Sequencing 16S rRNA genes (SSU) cloned from Aeromonas strains revealed that strains contained up to six copies differing by < or = 1.5%. The SSU copies from Aeromonas veronii LMG13695 clustered with sequences from four Aeromonas species. These results demonstrate intragenomic heterogeneity of SSU and suggest caution w...

journal_title：Journal of bacteriology

authors： Morandi A,Zhaxybayeva O,Gogarten JP,Graf J

更新日期：2005-09-01 00:00:00