Structure of complex flagellar filaments in Rhizobium meliloti.

Abstract:

:The complex flagella of Rhizobium meliloti 2011 and MVII-1 were analyzed with regard to serology, fine structure, subunits, and amino acid composition. The serological identities of flagellar filaments of the two strains were demonstrated by double immunodiffusion with antiflagellin antiserum. The filaments had a diameter of 16 nm. Their morphology was dominated by the prominent undulations of an external three-start helix running at a 10-nm axial distance and at an angle of 32 degrees. Faint nearly axial striations indicated the presence of a tubular core of a different helical order. The complex filaments consisted of 40,000-dalton flagellin monomers. Typically, the amino acid composition was 3 to 4% higher in nonpolar residues and 5 to 7% lower in aspartic and glutamic acids (and their amides) than that of plain flagellar proteins. There were no immunochemical relationships among Pseudomonas rhodos, Rhizobium lupini, and R. meliloti complex flagella, suggesting that the latter represent a new class.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Krupski G,Götz R,Ober K,Pleier E,Schmitt R

doi

10.1128/JB.162.1.361-366.1985

subject

Has Abstract

pub_date

1985-04-01 00:00:00

pages

361-6

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

162

pub_type

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