Abstract:
:In many microorganisms the first step for alkane degradation is the terminal oxidation of the molecule by an alkane hydroxylase. We report the characterization of a gene coding for an alkane hydroxylase in a Burkholderia cepacia strain isolated from an oil-contaminated site. The protein encoded showed similarity to other known or predicted bacterial alkane hydroxylases, although it clustered on a separate branch together with the predicted alkane hydroxylase of a Mycobacterium tuberculosis strain. Introduction of the cloned B. cepacia gene into an alkane hydroxylase knockout mutant of Pseudomonas fluorescens CHAO restored its ability to grow on alkanes, which confirms that the gene analyzed encodes a functional alkane hydroxylase. The gene, which was named alkB, is not linked to other genes of the alkane oxidation pathway. Its promoter was identified, and its expression was analyzed under different growth conditions. Transcription was induced by alkanes of chain lengths containing 12 to at least 30 carbon atoms as well as by alkanols. Although the gene was efficiently expressed during exponential growth, transcription increased about fivefold when cells approached stationary phase, a characteristic not shared by the few alkane degraders whose regulation has been studied. Expression of the alkB gene was under carbon catabolite repression when cells were cultured in the presence of several organic acids and sugars or in a complex (rich) medium. The catabolic repression process showed several characteristics that are clearly different from what has been observed in other alkane degradation pathways.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Marín MM,Smits TH,van Beilen JB,Rojo Fdoi
10.1128/JB.183.14.4202-4209.2001keywords:
subject
Has Abstractpub_date
2001-07-01 00:00:00pages
4202-9issue
14eissn
0021-9193issn
1098-5530journal_volume
183pub_type
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1969-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1975-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1987-08-01 00:00:00
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更新日期:2005-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1997-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.4.1489-1492.1987
更新日期:1987-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1489-1501.1975
更新日期:1975-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.2.298-304.1961
更新日期:1961-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.10.5567-5571.1989
更新日期:1989-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1964-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-01-01 00:00:00
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更新日期:1979-11-01 00:00:00
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更新日期:1994-08-01 00:00:00
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更新日期:2010-08-01 00:00:00
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更新日期:1986-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2006-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2017-08-14 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-01-01 00:00:00