Return of Streptococcus faecalis DNA cloned in Escherichia coli to its original host via transformation of Streptococcus sanguis followed by conjugative mobilization.

Abstract:

:Cloning vectors were introduced into Streptococcus faecalis by conjugation. A conjugative plasmid (pVA797) and cloning vector pVA838 recombined in Streptococcus sanguis at homologous sequences, forming a cointegrate. The pVA797::pVA838 cointegrate transferred to S. faecalis by conjugation. Recombination between homologous sequences resolved the cointegrate in the S. faecalis transconjugants, and pVA797 and pVA838 segregated because of incompatibility. S. faecalis strains that received pVA838 by this mechanism contained plasmids indistinguishable from authentic pVA838 from Escherichia coli. Other plasmids, including pVA736, were introduced into S. faecalis by this method. This approach should facilitate the introduction of cloned DNA into S. faecalis.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Smith MD,Clewell DB

doi

10.1128/JB.160.3.1109-1114.1984

subject

Has Abstract

pub_date

1984-12-01 00:00:00

pages

1109-14

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

160

pub_type

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