The C-Terminal Zwitterionic Sequence of CotB1 Is Essential for Biosilicification of the Bacillus cereus Spore Coat.


UNLABELLED:Silica is deposited in and around the spore coat layer of Bacillus cereus, and enhances the spore's acid resistance. Several peptides and proteins, including diatom silaffin and silacidin peptides, are involved in eukaryotic silica biomineralization (biosilicification). Homologous sequence search revealed a silacidin-like sequence in the C-terminal region of CotB1, a spore coat protein of B. cereus. The negatively charged silacidin-like sequence is followed by a positively charged arginine-rich sequence of 14 amino acids, which is remarkably similar to the silaffins. These sequences impart a zwitterionic character to the C terminus of CotB1. Interestingly, the cotB1 gene appears to form a bicistronic operon with its paralog, cotB2, the product of which, however, lacks the C-terminal zwitterionic sequence. A ΔcotB1B2 mutant strain grew as fast and formed spores at the same rate as wild-type bacteria but did not show biosilicification. Complementation analysis showed that CotB1, but neither CotB2 nor C-terminally truncated mutants of CotB1, could restore the biosilicification activity in the ΔcotB1B2 mutant, suggesting that the C-terminal zwitterionic sequence of CotB1 is essential for the process. We found that the kinetics of CotB1 expression, as well as its localization, correlated well with the time course of biosilicification and the location of the deposited silica. To our knowledge, this is the first report of a protein directly involved in prokaryotic biosilicification. IMPORTANCE:Biosilicification is the process by which organisms incorporate soluble silicate in the form of insoluble silica. Although the mechanisms underlying eukaryotic biosilicification have been intensively investigated, prokaryotic biosilicification was not studied until recently. We previously demonstrated that biosilicification occurs in Bacillus cereus and its close relatives, and that silica is deposited in and around a spore coat layer as a protective coating against acid. The present study reveals that a B. cereus spore coat protein, CotB1, which carried a C-terminal zwitterionic sequence, is essential for biosilicification. Our results provide the first insight into mechanisms required for biosilicification in prokaryotes.


J Bacteriol


Journal of bacteriology


Motomura K,Ikeda T,Matsuyama S,Abdelhamid MA,Tanaka T,Ishida T,Hirota R,Kuroda A




Has Abstract


2015-10-26 00:00:00














  • SpoVM, a small protein essential to development in Bacillus subtilis, interacts with the ATP-dependent protease FtsH.

    abstract::The spoVM gene encodes a 26-amino-acid polypeptide that is essential for spore formation in Bacillus subtilis. A transposon insertion within the spoVM open reading frame has been shown to encode a chimeric protein which is biologically inactive and produces a phenotype identical to that of a deletion and insertion mut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Cutting S,Anderson M,Lysenko E,Page A,Tomoyasu T,Tatematsu K,Tatsuta T,Kroos L,Ogura T

    更新日期:1997-09-01 00:00:00

  • Detoxification of protoanemonin by dienelactone hydrolase.

    abstract::Protoanemonin is a toxic metabolite which may be formed during the degradation of some chloroaromatic compounds, such as polychlorinated biphenyls, by natural microbial consortia. We show here that protoanemonin can be transformed by dienelactone hydrolase of Pseudomonas sp. strain B13 to cis-acetylacrylate. Although ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Brückmann M,Blasco R,Timmis KN,Pieper DH

    更新日期:1998-01-01 00:00:00

  • Alteration of tryptophan-mediated regulation in Neurospora crassa by indoleglycerol phosphate.

    abstract::The accumulation of imidazoleglycerol phosphate during growth of Neurospora crassa in the presence of 3-amino-1,2,4-triazole was found to cause derepression of tryptophan synthetase and to inhibit the induction of kynureninase. Accumulation of indoleglycerol phosphate in response to growth in the presence of indole ac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Turner JR,Matchett WH

    更新日期:1968-05-01 00:00:00

  • Cloning, sequences, and characterization of two chitinase genes from the Antarctic Arthrobacter sp. strain TAD20: isolation and partial characterization of the enzymes.

    abstract::Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA and ChiB (ArChiA and ArChiB), in response to chitin induction. A single chromosomal DNA fragment containing the genes c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lonhienne T,Mavromatis K,Vorgias CE,Buchon L,Gerday C,Bouriotis V

    更新日期:2001-03-01 00:00:00

  • CTX prophages in classical biotype Vibrio cholerae: functional phage genes but dysfunctional phage genomes.

    abstract::CTXphi is a filamentous, lysogenic bacteriophage whose genome encodes cholera toxin, the primary virulence factor produced by Vibrio cholerae. CTX prophages in O1 El Tor and O139 strains of V. cholerae are found within arrays of genetically related elements integrated at a single locus within the V. cholerae large chr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Davis BM,Moyer KE,Boyd EF,Waldor MK

    更新日期:2000-12-01 00:00:00

  • Physiological and biochemical characterization of the soluble formate dehydrogenase, a molybdoenzyme from Alcaligenes eutrophus.

    abstract::Organoautotrophic growth of Alcaligenes eutrophus on formate was dependent on the presence of molybdate in the medium. Supplementation of the medium with tungstate lead to growth cessation. Corresponding effects of these anions were observed for the activity of the soluble, NAD(+)-linked formate dehydrogenase (S-FDH; ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Friedebold J,Bowien B

    更新日期:1993-08-01 00:00:00

  • Identification of crucial amino acids of bacterial Peptide deformylases affecting enzymatic activity in response to oxidative stress.

    abstract::Peptide deformylase (PDF) is an essential bacterial metalloprotease involved in deformylation of N-formyl group from nascent polypeptide chains during protein synthesis. Iron-containing variants of this enzyme from Salmonella enterica serovar Typhimurium (sPDF) and Mycobacterium tuberculosis (mPDF), although inhibited...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kumar S,Kanudia P,Karthikeyan S,Chakraborti PK

    更新日期:2014-01-01 00:00:00

  • The MscS and MscL families of mechanosensitive channels act as microbial emergency release valves.

    abstract::Single-celled organisms must survive exposure to environmental extremes. Perhaps one of the most variable and potentially life-threatening changes that can occur is that of a rapid and acute decrease in external osmolarity. This easily translates into several atmospheres of additional pressure that can build up within...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审


    authors: Booth IR,Blount P

    更新日期:2012-09-01 00:00:00


    abstract::Altenbern, Robert A. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.). Growth of L forms of streptomycin-dependent Proteus mirabilis. J. Bacteriol. 85:619-622. 1963.-A streptomycin-dependent mutant of Proteus mirabilis produces L forms on penicillin-containing medium when dihydrostreptomycin is also p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: ALTENBERN RA

    更新日期:1963-03-01 00:00:00

  • VirB1* promotes T-pilus formation in the vir-Type IV secretion system of Agrobacterium tumefaciens.

    abstract::The vir-type IV secretion system of Agrobacterium is assembled from 12 proteins encoded by the virB operon and virD4. VirB1 is one of the least-studied proteins encoded by the virB operon. Its N terminus is a lytic transglycosylase. The C-terminal third of the protein, VirB1*, is cleaved from VirB1 and secreted to the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Zupan J,Hackworth CA,Aguilar J,Ward D,Zambryski P

    更新日期:2007-09-01 00:00:00

  • Absence of correlation between rates of cell wall turnover and autolysis shown by Bacillus subtilis mutants.

    abstract::Bacillus subtilis mutants with reduced rates of cell wall autolysis reached a constant rate of wall turnover after a longer lag than the standard strain but eventually showed the same turnover rate. In reverse, a turnover-deficient mutant autolysed at a slightly higher rate than the standard strain. Consequently, ther...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Vitković L,Cheung HY,Freese E

    更新日期:1984-01-01 00:00:00

  • Transformation of leucine and rifampin traits in Neisseria gonorrhoeae with deoxyribonucleic acid from homologous and heterologous origins.

    abstract::A leucine-requiring, rifampin-sensitive strain of Neisseria gonorrhoeae was transformed to a leucine-nonrequiring, rifampin-resistant phenotype with deoxyribonucleic acid (DNA) obtained from both N. meningitidis and N. gonorrhoeae. The transforming efficiency of the meningococcal DNA was about 10- to 100-fold less tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wood DO,Brownell GH

    更新日期:1975-02-01 00:00:00

  • Purification and characterization of ribitol-5-phosphate and xylitol-5-phosphate dehydrogenases from strains of Lactobacillus casei.

    abstract::A simple three-step procedure is described which yields electrophoretically homogeneous preparations of ribitol-5-phosphate dehydrogenase and xylitol-5-phosphate dehydrogenase. The former enzyme is a 115,000-molecular-weight protein composed of two subunits of identical size and is specific for its substrate, ribitol....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hausman SZ,London J

    更新日期:1987-04-01 00:00:00

  • Levels of enzymes involved in the synthesis of acetate from CO2 in Clostridium thermoautotrophicum.

    abstract::The acetogenic bacterium Clostridium thermoautotrophicum, grown on methanol, glucose, or CO2-H2, contained high levels of corrinoids, formate dehydrogenase, tetrahydrofolate enzymes, carbon monoxide dehydrogenase, and hydrogenase. Cell-free extracts catalyzed pyruvate-dependent formation of acetate from methyltetrahyd...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Clark JE,Ragsdale SW,Ljungdahl LG,Wiegel J

    更新日期:1982-07-01 00:00:00


    abstract::Dienes, Louis (Massachusetts General Hospital, Boston, Mass.), Bojana M. Bandur, and Sarabelle Madoff. Development of L-type growth in Neisseria gonorrhoeae cultures. J. Bacteriol. 87:1471-1476. 1964.-Strains of Neisseria gonorrhoeae (22) were studied immediately after isolation from patients. After 24 to 48 hr of inc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1964-06-01 00:00:00

  • Gastrointestinal microflora of mutton birds (Puffinus tenuirostris) in relation to "limy" disease.

    abstract::Mushin, Rose (University of Melbourne, Victoria, Australia) and Frances M. Ashburner. Gastrointestinal microflora of mutton birds (Puffinus tenuirostris) in relation to "limy" disease. J. Bacteriol. 83:1260-1267. 1962.-The aerobic bacterial intestinal flora of mutton birds, healthy and affected with "limy" disease, wa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1962-06-01 00:00:00

  • Probing the role of cysteine residues in glucosamine-1-phosphate acetyltransferase activity of the bifunctional GlmU protein from Escherichia coli: site-directed mutagenesis and characterization of the mutant enzymes.

    abstract::The glucosamine-1-phosphate acetyltransferase activity but not the uridyltransferase activity of the bifunctional GlmU enzyme from Escherichia coli was lost when GlmU was stored in the absence of beta-mercaptoethanol or incubated with thiol-specific reagents. The enzyme was protected from inactivation in the presence ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pompeo F,van Heijenoort J,Mengin-Lecreulx D

    更新日期:1998-09-01 00:00:00

  • The A1A0 ATPase from Methanosarcina mazei: cloning of the 5' end of the aha operon encoding the membrane domain and expression of the proteolipid in a membrane-bound form in Escherichia coli.

    abstract::Three additional ATPase genes, clustered in the order ahaH, ahaI, and ahaK, were found upstream of the previously characterized genes ahaECFABDG coding for the archaeal A1A0 ATPase from Methanosarcina mazei. ahaH, the first gene in the cluster, is preceded by a conserved promoter sequence. Northern blot analysis revea...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ruppert C,Wimmers S,Lemker T,Müller V

    更新日期:1998-07-01 00:00:00

  • The RNA polymerase alpha subunit from Sinorhizobium meliloti can assemble with RNA polymerase subunits from Escherichia coli and function in basal and activated transcription both in vivo and in vitro.

    abstract::Sinorhizobium meliloti, a gram-negative soil bacterium, forms a nitrogen-fixing symbiotic relationship with members of the legume family. To facilitate our studies of transcription in S. meliloti, we cloned and characterized the gene for the alpha subunit of RNA polymerase (RNAP). S. meliloti rpoA encodes a 336-amino-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Peck MC,Gaal T,Fisher RF,Gourse RL,Long SR

    更新日期:2002-07-01 00:00:00

  • A family of insertion sequences that impacts integrons by specific targeting of gene cassette recombination sites, the IS1111-attC Group.

    abstract::Integrons facilitate the evolution of complex phenotypes by physical and transcriptional linkage of genes. They can be categorized as chromosomal integrons (CIs) or mobile resistance integrons (MRIs). The significance of MRIs for the problem of multiple antibiotic resistance is well established. CIs are more widesprea...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tetu SG,Holmes AJ

    更新日期:2008-07-01 00:00:00

  • Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12.

    abstract::We have cloned and sequenced a cluster of six open reading frames containing gene kdsA from Escherichia coli K-12. The gene encodes 3-deoxy-D-manno-octulosonate 8-phosphate synthetase (KDO-8-phosphate synthetase), which catalyzes formation of 3-deoxy-D-manno-octulosonic acid (KDO), an essential component of enterobact...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Strohmaier H,Remler P,Renner W,Högenauer G

    更新日期:1995-08-01 00:00:00

  • cse15, cse60, and csk22 are new members of mother-cell-specific sporulation regulons in Bacillus subtilis.

    abstract::We report on the characterization of three new transcription units expressed during sporulation in Bacillus subtilis. Two of the units, cse15 and cse60, were mapped at about 123 degrees and 62 degrees on the genetic map, respectively. Their transcription commenced around h 2 of sporulation and showed an absolute requi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Henriques AO,Bryan EM,Beall BW,Moran CP Jr

    更新日期:1997-01-01 00:00:00

  • Suppression of the defects in rdgB mutants of Escherichia coli K-12 by the cloned purA gene.

    abstract::A gene suppressing the defects of Escherichia coli rdgB (recA-dependent growth) and recA(Ts) rdgB mutants was cloned. The cloned gene suppresses the hyper-Rec phenotype and the enhanced level of SOS functions in rdgB mutants at the nonpermissive temperature. We identified the cloned gene as purA. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Clyman J,Cunningham RP

    更新日期:1991-02-01 00:00:00

  • Deletion strains reveal metabolic roles for key elemental sulfur-responsive proteins in Pyrococcus furiosus.

    abstract::Transcriptional and enzymatic analyses of Pyrococcus furiosus previously indicated that three proteins play key roles in the metabolism of elemental sulfur (S(0)): a membrane-bound oxidoreductase complex (MBX), a cytoplasmic coenzyme A-dependent NADPH sulfur oxidoreductase (NSR), and sulfur-induced protein A (SipA). D...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bridger SL,Clarkson SM,Stirrett K,DeBarry MB,Lipscomb GL,Schut GJ,Westpheling J,Scott RA,Adams MW

    更新日期:2011-12-01 00:00:00

  • Purification and properties of mutant and wild-type diaphorases from Diplococcus pneumoniae.

    abstract::Optochin-resistant mutant and wild-type diaphorases were purified approximately 300-fold by a combination of batch adsorption and column chromatography with diethylaminoethyl cellulose, and were characterized with regard to their pH optima, sensitivity to optochin inhibition and heat inactivation, Michaelis constants ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kreger AS,Olsen RH

    更新日期:1968-10-01 00:00:00

  • Growth phase and pH influence peptide signaling for competence development in Streptococcus mutans.

    abstract::The development of competence by the dental caries pathogen Streptococcus mutans is mediated primarily through the alternative sigma factor ComX (SigX), which is under the control of multiple regulatory systems and activates the expression of genes involved in DNA uptake and recombination. Here we report that the indu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Guo Q,Ahn SJ,Kaspar J,Zhou X,Burne RA

    更新日期:2014-01-01 00:00:00

  • Actin-binding proteins from Burkholderia mallei and Burkholderia thailandensis can functionally compensate for the actin-based motility defect of a Burkholderia pseudomallei bimA mutant.

    abstract::Recently we identified a bacterial factor (BimA) required for actin-based motility of Burkholderia pseudomallei. Here we report that Burkholderia mallei and Burkholderia thailandensis are capable of actin-based motility in J774.2 cells and that BimA homologs of these bacteria can restore the actin-based motility defec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Stevens JM,Ulrich RL,Taylor LA,Wood MW,Deshazer D,Stevens MP,Galyov EE

    更新日期:2005-11-01 00:00:00

  • Bacillus subtilis gene involved in cell division, sporulation, and exoenzyme secretion.

    abstract::Bacillus subtilis strains carrying div-341 or sacU mutations, or both, have been characterized to reveal the roles of both genes in the initiation of sporulation, as well as in cell division and exoenzyme secretion. Both mutations were closely linked by transformation and caused the pleiotropic effects on sporulation ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sadaie Y,Kada T

    更新日期:1985-08-01 00:00:00

  • Mutational analysis of Agrobacterium tumefaciens virD2: tyrosine 29 is essential for endonuclease activity.

    abstract::Agrobacterium tumefaciens VirD2 polypeptide, in the presence of VirD1, catalyzes a site- and strand-specific nicking reaction at the T-DNA border sequences. VirD2 is found tightly attached to the 5' end of the nicked DNA. The protein-DNA complex is presumably formed via a tyrosine residue of VirD2 (F. Durrenberger, A....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Vogel AM,Das A

    更新日期:1992-01-01 00:00:00

  • Protoplast formation and leakage of intramembrane cell components: induction by the competence activator substance of pneumococci.

    abstract::Treatment of pneumococci with activator (a protein that induces bacterial "competence" to absorb deoxyribonucleic acid molecules and undergo genetic transformation) can cause either protoplast formation or leakage of intracellular components to the medium depending on postincubation conditions. The leaked intracellula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Seto H,Tomasz A

    更新日期:1975-01-01 00:00:00