Abstract:
:After thermal denaturation, an in vivo-labeled RNA was found in a temperature-sensitive initiation mutant of Bacillus subtilis (dna-37) associated with high-molecular-weight DNA. This RNA could be clearly distinguished from other RNA species by different techniques of separation, such as Sepharose 2B filtration, chromatography on nitrocellulose, and equilibrium centrifugation in density gradient. It was obtained even when HCHO was present during denaturation and chilling of nucleic acids and was still detected after a second denaturation as well as after incubation with proteinase K. Properties of the complex were not altered by prior treatment with RNase H. A control experiment using two samples of the complex treated either with pancreatic DNase or with pancreatic RNase, denatured together and centrifuged in the same density gradient, showed that no artifactual associations occur between the DNA and the RNA components of the complex. These results demonstrate that the DNA and RNA in the complex are associated by neither hydrogen bonds nor proteins, but are indicative of a DNA-RNA covalent linkage. In addition, during synchronous replication after a previous period at a nonpermissive temperature, DNA-linked RNA synthesis took place at specific times which coincided with the appearance of rifampin resistance of the first and the second replication cycles. A possible involvement of this RNA in the initiation of chromosome replication is discussed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Henckes G,Vannier F,Buu A,Seror-Laurent SJdoi
10.1128/JB.149.1.79-91.1982subject
Has Abstractpub_date
1982-01-01 00:00:00pages
79-91issue
1eissn
0021-9193issn
1098-5530journal_volume
149pub_type
杂志文章abstract::DNA uptake by natural competence is a central process underlying the genetic plasticity, biology, and virulence of the human respiratory opportunistic pathogen Streptococcus pneumoniae A study reported in this issue (J. Slager, R. Aprianto, and J.-W. Veening, J. Bacteriol. 201:e00780-18, https://doi.org/10.1128/JB.007...
journal_title:Journal of bacteriology
pub_type: 评论,杂志文章
doi:10.1128/JB.00238-19
更新日期:2019-06-10 00:00:00
abstract::Diploid Saccharomyces cerevisiae cells induce YVH1 expression and enter the developmental pathway, leading to sporulation when starved for nitrogen. We show that yvh1 disruption causes a defect in spore maturation; overexpression of MCK1 or IME1 suppresses this yvh1 phenotype. While mck1 mutations are epistatic to tho...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.17.5219-5224.1999
更新日期:1999-09-01 00:00:00
abstract::In Salmonella enterica, the biosynthetic pathways for the generation of purines and the essential cofactor thiamine pyrophosphate branch after sharing five enzymatic steps. Phosphoribosyl amine (PRA) is the first intermediate in the common portion of the pathway and is generated from phosphoribosylpyrophosphate and gl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00745-06
更新日期:2006-10-01 00:00:00
abstract::Phase variation is important in bacterial pathogenesis, since it generates antigenic variation for the evasion of immune responses and provides a strategy for quick adaptation to environmental changes. In this study, a Helicobacter pylori clone, designated MOD525, was identified that displayed phase-variable lacZ expr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.23.6615-6624.2002
更新日期:2002-12-01 00:00:00
abstract::Malmgren, Richard A. (National Cancer Institute; Bethesda, Md.), Alan S. Rabson, Paula G. Carney, and Frances J. Paul. Immunofluorescence of green monkey kidney cells infected with adenovirus 12 and with adenovirus 12 plus simian virus 40. J. Bacteriol. 91:262-265. 1966.-Immunofluorescence studies of the viral antigen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.1.262-265.1966
更新日期:1966-01-01 00:00:00
abstract::Site-directed mutagenesis has been employed to address the functional significance of the highly conserved aspartic and glutamic acid residues present in the Walker B (also called motif II) sequence in Escherichia coli DNA helicase II. Two mutant proteins, UvrDE221Q and UvrDD220NE221Q, were expressed and purified to a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.19.5612-5621.1995
更新日期:1995-10-01 00:00:00
abstract::Mycoplasma pneumoniae harvested from media which had become acid lost the ability both to induce formation of tetrazolium reduction inhibition antibody and to act as antigens in immunodiffusion against human convalescent-phase sera. Incorporation of N-tris(hydroxymethyl)-2-aminoethane sulfonic acid and N-2-hydroxyethy...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.2.612-619.1969
更新日期:1969-02-01 00:00:00
abstract::A procedure is described for the purification of the Escherichia coli outer membrane (lipopolysaccharide or L membrane) with flagella still attached. The resulting lipopolysaccharide membrane was in the form of vesicles that had a trilaminar structure in thin section and contained about 55% lipopolysaccharide and 45% ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.396-407.1971
更新日期:1971-01-01 00:00:00
abstract::Integrative and conjugative elements (ICEs), also called conjugative transposons, are genomic islands that excise, self-transfer by conjugation, and integrate in the genome of the recipient bacterium. The current investigation shows the intraspecies conjugative transfer of the first described ICEs in Streptococcus the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01412-08
更新日期:2009-04-01 00:00:00
abstract::Genetically disabled lambda plac5 transducing phage derivatives were used to study the recB dependence of recombination during specialized transduction. The frequency of transduction was normalized to colony-forming units, and the end product of recombination was monitored by scoring for addition and substitution tran...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.3.1485-1488.1982
更新日期:1982-06-01 00:00:00
abstract::The abundance of oxidants and reductants must be balanced for an organism to thrive. Bacteria have evolved methods to prevent redox imbalances and to mitigate their deleterious consequences through the expression of detoxification enzymes, antioxidants, and systems to repair or degrade damaged proteins and DNA. Regula...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00128-18
更新日期:2018-08-10 00:00:00
abstract::Strain C5.84 is a Tn5-751 insertion mutant of Aeromonas hydrophila which is unable to secrete extracellular proteins, instead accumulating them in the periplasm (B. Jiang and S.P. Howard, J. Bacteriol. 173:1241-1249, 1991). A 3.5-kb BglII fragment which complements this mutation was isolated from the chromosome of the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.22.6819-6826.1994
更新日期:1994-11-01 00:00:00
abstract::Genetic and biochemical evidence was obtained for lysine catabolism via cadaverine and delta-aminovalerate in both the beta-lactam producer Streptomyces clavuligerus and the nonproducer Streptomyces lividans. This pathway is used when lysine is supplied as the sole source of nitrogen for the organism. A second pathway...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.1.299-302.1989
更新日期:1989-01-01 00:00:00
abstract::Bam35, a temperate double-stranded DNA bacteriophage with a 15-kb linear genome, infects gram-positive Bacillus thuringiensis cells. Bam35 morphology and genome organization resemble those of PRD1, a lytic phage infecting gram-negative bacteria. Bam35 and PRD1 have an outer protein coat surrounding a membrane that enc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.10.3521-3527.2005
更新日期:2005-05-01 00:00:00
abstract::Cytochrome bd oxidase operons from more than 50 species of bacteria contain a short gene encoding a small protein that ranges from ∼30 to 50 amino acids and is predicted to localize to the cell membrane. Although cytochrome bd oxidases have been studied for more than 70 years, little is known about the role of this sm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00324-13
更新日期:2013-08-01 00:00:00
abstract::An analysis of the turnover of the major phospholipids of Micrococcus cerificans growing or nongrowing cultures. The turnover rates of (14)C-PE and (14)C-PE were 61.5% of the total phospholipid, exhibited no significant rate of turnover in either growing or nongrowing cultures. The turnover rates of PE-(14)C and PE-(3...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.3.806-814.1971
更新日期:1971-09-01 00:00:00
abstract::High-pressure liquid chromatography of a muramidase digest of murein sacculi from Caulobacter crescentus showed that the absence of D-alanine carboxypeptidase activity in the cells was reflected by a very high content of pentapeptide in the murein. Approximately half of the pentapeptide side chains were shown to conta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.2.649-655.1983
更新日期:1983-11-01 00:00:00
abstract::Rhodococcus sp. strain DK17 is capable of utilizing various derivatives of benzene and bicyclics containing both aromatic and alicyclic moieties as sole carbon and energy sources. Here, we present the 9,107,362-bp draft genome sequence of DK17 and its genomic analysis in comparison with other members of the genus Rhod...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00844-12
更新日期:2012-08-01 00:00:00
abstract::Temperature-sensitive nalA mutants of Escherichia coli have been used to investigate the structure and functions of deoxyribonucleic acid (DNA) gyrase. Extracts of one such mutant (nalA43) had thermosensitive DNA gyrase subunit A activity but normal gyrase subunit B activity, proving definitively that nalA is the stru...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.424-435.1979
更新日期:1979-11-01 00:00:00
abstract::Exfoliative toxin type B is produced by bacteriophage group II strains of Staphylococcus aureus and is a causative agent of staphylococcal scalded-skin syndrome. In addition to exfoliative toxin B, most isolates also produce a bacteriocin and are immune to the action of the bacteriocin. These phenotypes, as well as re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.2.574-580.1986
更新日期:1986-05-01 00:00:00
abstract::Rhizobactin 1021 is a hydroxymate siderophore produced by the soil bacterium Sinorhizobium meliloti 2011. A regulon comprising rhtA, encoding the outer membrane receptor protein for the ferrisiderophore; the biosynthesis operon rhbABCDEF; and rhrA, the Ara-C-like regulator of the receptor and biosynthesis genes has be...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.10.2996-3005.2004
更新日期:2004-05-01 00:00:00
abstract::We previously showed that adsorption of bacteriophages T4 and T5 to their respective outer membrane receptors induced a partial depolarization of the cytoplasmic membrane. As these membrane potential changes were independent of phage properties, we proposed that phage adsorption triggered the emission of a signal whic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.3.789-794.1984
更新日期:1984-03-01 00:00:00
abstract::The gene (bdb) for protein thiol-disulfide oxidoreductase cloned from Bacillus brevis was found to encode a polypeptide consisting of 117 amino acid residues with a signal peptide of 27 residues. Bdb contains a well-conserved motif, Cys-X-X-Cys, which functions as the active center of disulfide oxidoreductases such as...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.3.745-749.1995
更新日期:1995-02-01 00:00:00
abstract::Transposon mutagenesis was used to isolate mutants of Aeromonas hydrophila which were deficient in the production of extracellular proteins. The culture supernatants of two of the mutants were essentially devoid of the proteins normally secreted by the parent strain, despite their continued synthesis. Western immunobl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.3.1241-1249.1991
更新日期:1991-02-01 00:00:00
abstract::Thrust for propulsion of flagellated bacteria is generated by rotation of a propeller, the flagellum. The power to drive the polar flagellar rotary motor of Vibrio parahaemolyticus is derived from the transmembrane potential of sodium ions. Force is generated by the motor on coupling of the movement of ions across the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.19.5988-5998.1994
更新日期:1994-10-01 00:00:00
abstract::A novel of Escherichia coli endopeptidase was used for a selective partial hydrolysis of the peptide bridges which interlink the glycan chains in E. coli sacculi. The loosening of the murein network revealed, in the electron microscope, a preferential orientation of the glycan chains, more or less perpendicular to the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.2.723-729.1978
更新日期:1978-11-01 00:00:00
abstract::The mobile element IS256 causes phase variation of biofilm formation in Staphylococcus epidermidis by insertion and precise excision from the icaADBC operon. Precise excision, i.e., removal of the target site duplications (TSDs) and restoration of the original DNA sequence, occurs rarely but independently of functiona...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01290-07
更新日期:2008-02-01 00:00:00
abstract::We developed a procedure for isolating membrane vesicles from the homolactic fermentative bacterium Streptococcus cremoris. The membrane vesicles were shown to have a right-side-out orientation by freeze-etch electron microscopy and to be free of cytoplasmic constituents. The membrane vesicles retained their functiona...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.733-738.1982
更新日期:1982-02-01 00:00:00
abstract::"Roseburia inulinivorans" is an anaerobic polysaccharide-utilizing firmicute bacterium from the human colon that was identified as a producer of butyric acid during growth on glucose, starch, or inulin. R. inulinivorans A2-194 is also able to grow on the host-derived sugar fucose, following a lag period, producing pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00137-06
更新日期:2006-06-01 00:00:00
abstract::We performed a systematic survey of transcription of a large region of the Rhizobium meliloti symbiotic plasmid pSym. This led to the discovery of two new sequences induced during symbiosis. The first sequence was linked to the known nitrogen fixation (nif-fix) gene cluster, and its expression depended on the nifA gen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.2239-2244.1987
更新日期:1987-05-01 00:00:00