Abstract:
:Cytochrome bd oxidase operons from more than 50 species of bacteria contain a short gene encoding a small protein that ranges from ∼30 to 50 amino acids and is predicted to localize to the cell membrane. Although cytochrome bd oxidases have been studied for more than 70 years, little is known about the role of this small protein, denoted CydX, in oxidase activity. Here we report that Escherichia coli mutants lacking CydX exhibit phenotypes associated with reduced oxidase activity. In addition, cell membrane extracts from ΔcydX mutant strains have reduced oxidase activity in vitro. Consistent with data showing that CydX is required for cytochrome bd oxidase activity, copurification experiments indicate that CydX interacts with the CydAB cytochrome bd oxidase complex. Together, these data support the hypothesis that CydX is a subunit of the CydAB cytochrome bd oxidase complex that is required for complex activity. The results of mutation analysis of CydX suggest that few individual amino acids in the small protein are essential for function, at least in the context of protein overexpression. In addition, the results of analysis of the paralogous small transmembrane protein AppX show that the two proteins could have some overlapping functionality in the cell and that both have the potential to interact with the CydAB complex.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
VanOrsdel CE,Bhatt S,Allen RJ,Brenner EP,Hobson JJ,Jamil A,Haynes BM,Genson AM,Hemm MRdoi
10.1128/JB.00324-13subject
Has Abstractpub_date
2013-08-01 00:00:00pages
3640-50issue
16eissn
0021-9193issn
1098-5530pii
JB.00324-13journal_volume
195pub_type
杂志文章abstract::Host range mutants of phage T1 (T1h), which productively infected tonB mutants of Escherichia coli, were isolated. The phage mutants were inactivated by isolated outer membranes of E. coli in contrast to the wild-type phage, which only adsorbed reversibly. For the infection process, the tonB function is apparently onl...
journal_title:Journal of bacteriology
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doi:10.1128/JB.135.1.190-197.1978
更新日期:1978-07-01 00:00:00
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doi:10.1128/JB.127.1.523-535.1976
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JB.123.3.862-870.1975
更新日期:1975-09-01 00:00:00
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更新日期:2005-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/jb.174.24.8094-8101.1992
更新日期:1992-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-04-01 00:00:00
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更新日期:1988-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.3.869-878.1999
更新日期:1999-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1992-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1972-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1981-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.11.2842-2848.1998
更新日期:1998-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-08-01 00:00:00
abstract::Clostridium perfringens food poisoning is caused by type A isolates carrying a chromosomal enterotoxin (cpe) gene (C-cpe), while C. perfringens-associated non-food-borne gastrointestinal (GI) diseases are caused by isolates carrying a plasmid-borne cpe gene (P-cpe). C. perfringens spores are thought to be the importan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.5.1320-1326.1967
更新日期:1967-11-01 00:00:00