Characterization of endonuclease III (nth) and endonuclease VIII (nei) mutants of Escherichia coli K-12.

abstract：:We have examined the uptake of [14C]octopine and [14C]nopaline by Agrobacterium tumefaciens strains containing the C58 chromosomal background in medium suitable for the induction of vir genes. All strains tested could transport both of these opines, regardless of the presence or type of Ti plasmid (octopine or nopalin...

journal_title：Journal of bacteriology

authors： Krishnan M,Burgner JW,Chilton WS,Gelvin SB

更新日期：1991-01-01 00:00:00

abstract：:Genetic transformation of Sulfolobus acidocaldarius by a multiply marked pyrE gene provided a high-resolution assay of homologous recombination in a hyperthermophilic archaeon. Analysis of 100 Pyr(+) transformants revealed that this recombination system could transfer each of 23 nonselected base pair substitutions to ...

journal_title：Journal of bacteriology

authors： Grogan DW,Rockwood J

更新日期：2010-09-01 00:00:00

abstract：:We have cloned a lipopolysaccharide (LPS) biosynthetic gene from Pseudomonas aeruginosa PAO1 that complements the defect in the production and incorporation of LPS O side chains in the LPS-rough strain AK1012. This gene was characterized by pulsed-field gel electrophoresis, deletion and restriction mapping of the clon...

journal_title：Journal of bacteriology

authors： Goldberg JB,Hatano K,Pier GB

更新日期：1993-03-01 00:00:00

abstract：:The group A streptococcus (GAS) Streptococcus pyogenes is known to cause self-limiting purulent infections in humans. The role of GAS pili in host cell adhesion and biofilm formation is likely fundamental in early colonization. Pilus genes are found in the FCT (fibronectin-binding protein, collagen-binding protein, an...

journal_title：Journal of bacteriology

authors： Kimura KR,Nakata M,Sumitomo T,Kreikemeyer B,Podbielski A,Terao Y,Kawabata S

更新日期：2012-02-01 00:00:00

abstract：:Growth of Lactococcus lactis in milk depends on the utilization of extracellular peptides. Up to now, oligopeptide uptake was thought to be due only to the ABC transporter Opp. Nevertheless, analysis of several Opp-deficient L. lactis strains revealed the implication of a second oligopeptide ABC transporter, the so-ca...

journal_title：Journal of bacteriology

authors： Lamarque M,Charbonnel P,Aubel D,Piard JC,Atlan D,Juillard V

更新日期：2004-10-01 00:00:00

abstract：:The properties of the anthranilate synthetase complex and its separated subunits were compared in catalyzing the anthranilate synthetase reaction, chorismate + l-glutamine or NH(4) (+) --> anthranilate, and the transferase reaction, anthranilate + 5'-phosphorylribosyl-1-pyrophosphate --> phosphoribosyl anthranilate. I...

journal_title：Journal of bacteriology

authors： Ito J,Yanofsky C

更新日期：1969-02-01 00:00:00

abstract：:Temperature induction of an Escherichia coli strains with lambda cI1857 integrated in the guaB gene has been used to produce strains containing chromosomal deletions extending into the xse and upp genes. By utilizing strains containing these deletions, it has been possible to order the genes in the guanine operon with...

journal_title：Journal of bacteriology

authors： Vales LD,Chase JW,Murphy JB

更新日期：1979-07-01 00:00:00

abstract：:We previously showed that dprA is required for efficient processing of linear DNA during cellular transformation in Haemophilus influenzae. In this study the transcriptional regulation of dprA and two downstream genes, dprB and dprC, is examined. We demonstrate by Northern blot analysis that the dprABC genes are trans...

journal_title：Journal of bacteriology

authors： Karudapuram S,Barcak GJ

更新日期：1997-08-01 00:00:00

abstract：:Intact cells of Bacillus stearothermophilus PV72 revealed, after conventional thin-sectioning procedures, the typical cell wall profile of S-layer-carrying gram-positive eubacteria consisting of a ca. 10-nm-thick peptidoglycan-containing layer and a ca. 10-nm-thick S layer. Cell wall preparations obtained by breaking ...

journal_title：Journal of bacteriology

authors： Breitwieser A,Gruber K,Sleytr UB

更新日期：1992-12-01 00:00:00

abstract：:Bacillus subtilis possesses different enzymes for the utilization of plant cell wall polysaccharides. This includes a gene cluster containing galactan degradation genes (ganA and ganB), two transporter component genes (ganQ and ganP), and the sugar-binding lipoprotein-encoding gene ganS (previously known as cycB). The...

journal_title：Journal of bacteriology

authors： Watzlawick H,Morabbi Heravi K,Altenbuchner J

更新日期：2016-09-22 00:00:00

abstract：:Escherichia coli possesses a soluble flavohemoglobin, with an unknown function, encoded by the hmp gene. A monolysogen containing an hmp-lacZ operon fusion was constructed to determine how the hmp promoter is regulated in response to heme ligands (O2, NO) or the presence of anaerobically utilized electron acceptors (n...

journal_title：Journal of bacteriology

authors： Poole RK,Anjum MF,Membrillo-Hernández J,Kim SO,Hughes MN,Stewart V

更新日期：1996-09-01 00:00:00

abstract：:Commonly used procedures effected the extraction of only 10% of the lipoteichoic acid of stationary-phase cells of Staphylococcus aureus and Streptococcus faecium, unless the cells were first disrupted. ...

journal_title：Journal of bacteriology

authors： Huff E

更新日期：1982-01-01 00:00:00

abstract：:Membrane vesicles isolated from competent cultures of Bacillus subtilis 168 bound up to 20 mug of double-stranded deoxyribonucleic acid (DNA) per mg of membrane protein in the presence of ethylenediaminetetraacetate. The formation of the DNA-membrane complex was time, temperature, and pH dependent. Eighty per cent of ...

journal_title：Journal of bacteriology

authors： Joenje H,Konings WN,Venema G

更新日期：1974-09-01 00:00:00

abstract：:Chlamydial lipopolysaccharide (LPS) agglutinated mouse and rabbit erythrocytes but not human, guinea pig, or pronghorn antelope erythrocytes. Hemagglutination was not specific for Chlamydia spp., as rough LPSs from Coxiella burnetii and Escherichia coli also agglutinated erythrocytes from the same animal species. Nona...

journal_title：Journal of bacteriology

authors： Watkins NG,Caldwell HD,Hackstadt T

更新日期：1987-08-01 00:00:00

abstract：:Twelve different dominant negative mutants of the Escherichia coli nucleoid-associated protein, H-NS, have been selected and characterized in vivo. The mutants are all severely defective in promoter repression activity in a strain lacking H-NS, and they all disrupt the repression normally exerted by H-NS at two of its...

journal_title：Journal of bacteriology

authors： Williams RM,Rimsky S,Buc H

更新日期：1996-08-01 00:00:00

abstract：:The ultrastructure of seven Thiobacillus species was studied. The structure of their cell envelopes is similar, if not identical, to that found in other gram-negative bacteria. Obvious differences were noted in the middle layer of the cell envelope of the seven cultures. Polyhedral inclusion bodies were apparent in fo...

journal_title：Journal of bacteriology

authors： Shively JM,Decker GL,Greenawalt JW

更新日期：1970-02-01 00:00:00

abstract：:Little is known about the effect of the host on the genetic stability of bacterial plant pathogens. Crown gall, a plant disease caused by Agrobacterium tumefaciens, may represent a useful model to study this effect. Indeed, our previous observations on the natural occurrence and origin of nonpathogenic agrobacteria su...

journal_title：Journal of bacteriology

authors： Bélanger C,Canfield ML,Moore LW,Dion P

更新日期：1995-07-01 00:00:00

abstract：:Oxalurate, the gratuitous inducer of the allantoin degradative enzymes, was taken into the cell by an energy-dependent active transport system with an apparent Km of 1.2 mM. Efflux of previously accumulated oxalurate was rapid, with a half-life of about 2 min. The oxalurate uptake system appears to be both constitutiv...

journal_title：Journal of bacteriology

authors： Cooper TG,McKelvey J,Sumrada R

更新日期：1979-09-01 00:00:00

abstract：:A new strategy was developed for rapid cloning of genes with a transposon mutation library. We constructed a transposon designated TnV that was derived from Tn5 and consists of the gene coding for neomycin phosphotransferase II as well as the replication origin of an Escherichia coli plasmid, pSC101, flanked by Tn5 in...

journal_title：Journal of bacteriology

authors： Furuichi T,Inouye M,Inouye S

更新日期：1985-10-01 00:00:00

abstract：:Transcriptional factor CarD is the only reported prokaryotic analog of eukaryotic high-mobility-group A (HMGA) proteins, in that it has contiguous acidic and AT hook DNA-binding segments and multifunctional roles in Myxococcus xanthus carotenogenesis and fruiting body formation. HMGA proteins are small, randomly struc...

journal_title：Journal of bacteriology

authors： Cayuela ML,Elías-Arnanz M,Peñalver-Mellado M,Padmanabhan S,Murillo FJ

更新日期：2003-06-01 00:00:00

abstract：:Thermococcus sp. strain 4557 is a hyperthermophilic anaerobic archaeon isolated from the deep-sea hydrothermal vent Guaymas Basin site in the Gulf of California at a depth of 2,000 m. Here, we present the complete genome sequence of Thermococcus sp. 4557, which consists of a single circular chromosome of 2,011,320 bp ...

journal_title：Journal of bacteriology

authors： Wang X,Gao Z,Xu X,Ruan L

更新日期：2011-10-01 00:00:00

abstract：:Austrian, Robert (The University of Pennsylvania School of Medicine, Philadelphia), and Patricia Collins. Importance of carbon dioxide in the isolation of pneumococci. J. Bacteriol. 92:1281-1284. 1966.-Of the strains of pneumococci isolated from man, 8% manifest a requirement for CO(2) if detectable growth is to occur...

journal_title：Journal of bacteriology

authors： Austrian R,Collins P

更新日期：1966-11-01 00:00:00

abstract：:Abram, Dinah (Purdue University, Lafayette, Ind.), A. E. Vatter, and Henry Koffler. Attachment and structural features of flagella of certain bacilli. J. Bacteriol. 91:2045-2068. 1966.-The attachment of flagella to cells of various mesophilic and thermophilic strains of Bacillus was studied electron microscopically. S...

journal_title：Journal of bacteriology

authors： Abram D,Vatter AE,Koffler H

更新日期：1966-05-01 00:00:00

abstract：:The coccoid cyanobacterium Chroococcidiopsis dominates microbial communities in the most extreme arid hot and cold deserts. These communities withstand constraints that result from multiple cycles of drying and wetting and/or prolonged desiccation, through mechanisms which remain poorly understood. Here we describe th...

journal_title：Journal of bacteriology

authors： Billi D,Friedmann EI,Helm RF,Potts M

更新日期：2001-04-01 00:00:00

abstract：:An aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646 was purified 196-fold by a combination of Mono-Q, Reactive Green 19 agarose affinity, and hydroxyapatite chromatographies. The purified enzyme runs as a single band of 140 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular ...

journal_title：Journal of bacteriology

authors： Li T,Rosazza JP

更新日期：1997-06-01 00:00:00

abstract：:A study was made to determine whether factors other than the availability of phosphorus were involved in the regulation of synthesis of teichoic and teichuronic acids in Bacillus subtilis subsp. niger WM. First, the nature of the carbon source was varied while the dilution rate was maintained at about 0.3 h-1. Irrespe...

journal_title：Journal of bacteriology

authors： Kruyssen FJ,de Boer WR,Wouters JT

更新日期：1980-10-01 00:00:00

abstract：:Bacteroides ruminicola B(1)4, a noncellulolytic rumen bacterium, produces an endoglucanase (carboxymethylcellulase [CMCase]) that is excreted into the culture supernatant. Cultures grown on glucose, fructose, maltose, mannose, and cellobiose had high specific activities of CMCase (greater than 3 mmol of reducing sugar...

journal_title：Journal of bacteriology

authors： Matsushita O,Russell JB,Wilson DB

更新日期：1990-07-01 00:00:00

abstract：:Shigella flexneri is a gram-negative bacterium that causes diarrhea and dysentery by invasion and spread through the colonic epithelium. Bacteria spread by assembling actin and other cytoskeletal proteins of the host into "actin tails" at the bacterial pole; actin tail assembly provides the force required to move bact...

journal_title：Journal of bacteriology

authors： Charles M,Magdalena J,Theriot JA,Goldberg MB

更新日期：1999-02-01 00:00:00

abstract：:Escherichia coli K-12, polAl(-) is a mutant strain whose extracts are deficient in Kornberg deoxyribonucleic acid (DNA) polymerase activity. We have compared the mutant and parental strains on the basis of a number of responses to ultraviolet (UV) and X-irradiation. For both types of radiation, the mutant is more sens...

journal_title：Journal of bacteriology

authors： Paterson MC,Boyle JM,Setlow RB

更新日期：1971-07-01 00:00:00

abstract：:The energetic efficiency of microbial growth is significantly reduced in cultures growing under glucose excess compared to cultures growing under glucose limitation, but the magnitude to which different energy-dissipating processes contribute to the reduced efficiency is currently not well understood. We introduce her...

journal_title：Journal of bacteriology

authors： Dauner M,Storni T,Sauer U

更新日期：2001-12-01 00:00:00