Abstract:
:Membrane vesicles isolated from competent cultures of Bacillus subtilis 168 bound up to 20 mug of double-stranded deoxyribonucleic acid (DNA) per mg of membrane protein in the presence of ethylenediaminetetraacetate. The formation of the DNA-membrane complex was time, temperature, and pH dependent. Eighty per cent of the DNA could be removed from the complex by treatment with deoxyribonuclease I. Nevertheless, the DNA that remained attached to the vesicles appeared to have been attacked by the enzyme, suggesting that all the complexed DNA is located at the outer surface of the vesicles. Pretreatment of DNA with deoxyribonuclease I destroyed its affinity for the vesicles. The extent of binding decreased by the addition of Mg(2+) ions, especially at high DNA concentrations (more than 2 mug/ml). This effect was partially due to membrane-associated Mg(2+)-dependent endonucleolytic activity, which caused double-strand breaks in addition to single-strand nicks, and to exonuclease activity. The endonucleolytic activity was enhanced by heating the membranes at 80 C. DNA-membrane association was not markedly affected by sulfhydryl reagents, but was largely inhibited by formaldehyde. Endogenous competence-stimulating activity did not alter the DNA-binding capacity of the vesicles.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Joenje H,Konings WN,Venema Gdoi
10.1128/JB.119.3.784-794.1974subject
Has Abstractpub_date
1974-09-01 00:00:00pages
784-94issue
3eissn
0021-9193issn
1098-5530journal_volume
119pub_type
杂志文章abstract::The Rhizobium meliloti bacA gene encodes a function that is essential for bacterial differentiation into bacteroids within plant cells in the symbiosis between R. meliloti and alfalfa. An Escherichia coli homolog of BacA, SbmA, is implicated in the uptake of microcin B17, microcin J25 (formerly microcin 25), and bleom...
journal_title:Journal of bacteriology
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1972-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.21.6732-6741.1991
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.7.1871-1878.1993
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1987-08-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1981-05-01 00:00:00
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更新日期:2006-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.377-382.1969
更新日期:1969-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.179.17.5534-5542.1997
更新日期:1997-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2015-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05660-11
更新日期:2011-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.17.5403-5405.1991
更新日期:1991-09-01 00:00:00
abstract::Expression of a circadian rhythm of conidiation in Neurospora crassa is enhanced in cultures grown on medium that is neutral or deficient in biotin. ...
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pub_type: 杂志文章
doi:10.1128/JB.123.1.387-389.1975
更新日期:1975-07-01 00:00:00