Importance of carbon dioxide in the isolation of pneumococci.

abstract：:Deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerase (EC 2.7.7.6) from Acinetobacter calcoaceticus was purified to apparent homogeneity and its properties were compared with those of the Escherichia coli B enzyme. The molecular weights of the two native active enzymes as well as their alpha and beta...

journal_title：Journal of bacteriology

authors： Kleppe RK,Kleppe K

更新日期：1976-02-01 00:00:00

abstract：:Two regulatory proteins, Gal repressor and isorepressor, control the expression of the gal and mgl operons in Escherichia coli. The transcription start sites for galR and galS, the genes for the repressor and isorepressor, were determined by primer extension of in vivo transcripts. Study of the promoter-lacZ gene fusi...

journal_title：Journal of bacteriology

authors： Weickert MJ,Adhya S

更新日期：1993-01-01 00:00:00

abstract：:Banerjea, A. (Rocky Mountain Laboratory, Hamilton, Mont.) and J. Munoz. Antigens of Bordetella pertussis. II, Purification of heat-labile toxin. J. Bacteriol. 84:269-274. 1962.-A mild method of separating heat-labile toxin of Bordetella pertussis from other cellular components is described; it consists of absorbing to...

journal_title：Journal of bacteriology

authors： BANERJEA A,MUNOZ J

更新日期：1962-08-01 00:00:00

abstract：:Deletion mutants of plasmid ColE1 that involve the replication origin and adjacent regions of the plasmid have been studied to determine the mechanism by which those mutations affect the expression of plasmid incompatibility. It was observed that (i) a region of ColE1 that is involved in the expression of plasmid inco...

journal_title：Journal of bacteriology

authors： Hashimoto-Gotoh T,Inselburg J

更新日期：1979-08-01 00:00:00

abstract：:A fatty acid Delta(9)-desaturase mutant of yeast was analyzed to establish the specificity of the fatty acid structural requirement. Several double-bond-containing and substituted fatty acids were tested. It was concluded that the presence of a Delta(9)cis double bond is necessary for growth. The need for a specific c...

journal_title：Journal of bacteriology

authors： Wisnieski BJ,Keith AD,Resnick MR

更新日期：1970-01-01 00:00:00

abstract：:An inorganic pyrophosphatase was purified over 600-fold to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme is a tetramer of Mr = 84,000, has a sedimentation coefficient of 5.8S, a Stokes radius of 3.5 nm, and an isoelectric point of 5.7. Like the enzyme of Escherichia coli, the pyrophosphatase ...

journal_title：Journal of bacteriology

authors： Verhoeven JA,Schenck KM,Meyer RR,Trela JM

更新日期：1986-10-01 00:00:00

abstract：:The type IV pili of plasmid R64 belonging to the type IVB group are required only for liquid mating. They consist of the major and minor components PilS pilin and PilV adhesin, respectively. PilS pilin is first synthesized as a 22-kDa prepilin from the pilS gene and is then processed to a 19-kDa mature pilin by PilU p...

journal_title：Journal of bacteriology

authors： Shimoda E,Muto T,Horiuchi T,Furuya N,Komano T

更新日期：2008-02-01 00:00:00

abstract：:We previously reported that the methicillin resistance gene mecA is carried by a novel type of mobile genetic element, SCCmec (staphylococcal cassette chromosome mec), in the chromosome of methicillin-resistant Staphylococcus aureus (MRSA). These elements are precisely excised from the chromosome and integrated into a...

journal_title：Journal of bacteriology

authors： Katayama Y,Takeuchi F,Ito T,Ma XX,Ui-Mizutani Y,Kobayashi I,Hiramatsu K

更新日期：2003-05-01 00:00:00

abstract：:Several phosphate-starvation-inducible (psi) genetic loci in Salmonella typhimurium were identified by fusing the lacZ gene to psi promoters by using the Mu d1 and Mu d1-8 bacteriophages. Although several different starvation conditions were examined, the psi loci responded solely to phosphate deprivation. A regulator...

journal_title：Journal of bacteriology

authors： Foster JW,Spector MP

更新日期：1986-05-01 00:00:00

abstract：:Primer extension analysis of transcripts of the Bacillus subtilis autolysin (cwlB) operon indicated that SigD-dependent transcripts from the Pd promoter are missing in the degU32(Hy) and degS200 (Hy) mutants. The degU32(Hy) mutation caused a 99% reduction in the expression of a sigD-lacZ translational fusion gene cons...

journal_title：Journal of bacteriology

authors： Tokunaga T,Rashid MH,Kuroda A,Sekiguchi J

更新日期：1994-08-01 00:00:00

abstract：:Azotobacter vinelandii presents a differentiation process leading to the formation of desiccation-resistant cysts. Alginate, the exopolysaccharide produced by this bacterium, has been postulated to have a role in cyst formation. Here, we report the cloning and characterization of the A. vinelandii gene coding for the ...

journal_title：Journal of bacteriology

authors： Campos M,Martínez-Salazar JM,Lloret L,Moreno S,Núñez C,Espín G,Soberón-Chávez G

更新日期：1996-04-01 00:00:00

abstract：:Spontaneous mutators of Salmonella typhimurium LT2 were generated by inserting the transposable element Tn5 or Tn10 into the bacterial chromosome. Two mutators mapped at the position of the mutH and mutL loci of S. typhimurium, and two other mutators mapped at positions corresponding to the mutS and uvrD loci of Esche...

journal_title：Journal of bacteriology

authors： Shanabruch WG,Behlau I,Walker GC

更新日期：1981-09-01 00:00:00

abstract：:During depletion of 4.5S RNA, cells of Escherichia coli displayed a heat shock response that was simultaneous with the first detectable effect on ribosome function and before major effects on cell growth. Either 4.5S RNA is involved directly in regulating the heat shock response, or this particular impairment of prote...

journal_title：Journal of bacteriology

authors： Bourgaize DB,Phillips TA,VanBogelen RA,Jones PG,Neidhardt FC,Fournier MJ

更新日期：1990-02-01 00:00:00

abstract：:The phs mutation, which causes a pleiotropic growth defect, has been mapped and shown to be an allele of rpoA, the gene for the alpha subunit of RNA polymerase. The mutation is shown to cause a transcription defect in the arabinose operon, araBAD. ...

journal_title：Journal of bacteriology

authors： Rowland GC,Giffard PM,Booth IR

更新日期：1985-11-01 00:00:00

abstract：:omega 4403 is the site of a Tn5 lac insertion in the Myxococcus xanthus genome that fuses lacZ expression to a developmentally regulated promoter. Cell-cell interactions that occur during development, including C-signaling, are required for expression of Tn5 lac omega 4403. We have cloned DNA upstream of the omega 440...

journal_title：Journal of bacteriology

authors： Fisseha M,Gloudemans M,Gill RE,Kroos L

更新日期：1996-05-01 00:00:00

abstract：:The gene encoding sigmaD, sigD, is transcribed from two promoter regions, the fla/che promoter region in front of the fla/che operon and PsigD directly in front of sigD. If sigmaD is translated from transcripts originating from PsigD, the cell is unable to express motility functions but synthesizes autolysins. Therefo...

journal_title：Journal of bacteriology

authors： Yang DH,von Kalckreuth J,Allmansberger R

更新日期：1999-05-01 00:00:00

abstract：:Scanning electron microscopy has been employed to examine events in the release and development of mitospores of the aquatic fungus, Allomyces arbuscula. Among the salient features of spore release from the mitosporangium is the digestion of the inner matrix of the exit papillum. Hydrolysis appears to begin at the out...

journal_title：Journal of bacteriology

authors： Seale TW,Delehanty J,Runyan RB

更新日期：1974-12-01 00:00:00

abstract：:Many genomes of anaerobic hyperthermophiles encode multiple homologs of NAD(P)H oxidase that are thought to function in response to oxidative stress. We investigated one of the seven NAD(P)H oxidase homologs (TK1481) in the sulfur-reducing hyperthermophilic archaeon Thermococcus kodakarensis, focusing on the catalytic...

journal_title：Journal of bacteriology

authors： Kobori H,Ogino M,Orita I,Nakamura S,Imanaka T,Fukui T

更新日期：2010-10-01 00:00:00

abstract：:Two low-molecular-weight basic proteins, termed A and B proteins, comprise about 15% of the protein of dormant spores of Bacillus megaterium. Irradiation of intact dormant spores with ultraviolet light results in covalent cross-linking of the A and B proteins to other spore macromolecules. The cross-linked A and B pro...

journal_title：Journal of bacteriology

authors： Setlow B,Setlow P

更新日期：1979-08-01 00:00:00

abstract：:Chloroethylthiamine was found to inhibit an entrapment of thiamine as thiamine monophosphate by blocking thiamine monophosphokinase in the cytoplasm after thiamine was taken up by the cells of Escherichia coli. ...

journal_title：Journal of bacteriology

authors： Iwashima A,Nose Y

更新日期：1972-12-01 00:00:00

abstract：:A nonswarming Serratia liquefaciens mutant deficient in serrawettin W2 production was constructed by transposon mutagenesis. Sequence homology indicated that insertion had occurred in gene swrA, which encodes a putative peptide synthetase. Expression of swrA is controlled by quorum sensing. ...

journal_title：Journal of bacteriology

authors： Lindum PW,Anthoni U,Christophersen C,Eberl L,Molin S,Givskov M

更新日期：1998-12-01 00:00:00

abstract：:Kasugamycin-resistant mutants of Bacillus subtilis were isolated and classified into two groups, one of which had resistance to kasugamycin in in vitro protein synthesis and mapped in the ribosomal region. The other group had no resistance to kasugamycin in in vitro protein synthesis and had weak cross-resistance to g...

journal_title：Journal of bacteriology

authors： Tominaga A,Kobayashi Y

更新日期：1978-09-01 00:00:00

abstract：:When an Escherichia coli K-12 culture was starved for glucose, 50% of the cells lost viability in about 6 days. When a K-12 mutant lacking five distinct peptidase activities, CM89, was starved in the same manner, viability was lost much more rapidly; 50% of the cells lost viability in about 2 days, whereas a parent st...

journal_title：Journal of bacteriology

authors： Reeve CA,Bockman AT,Matin A

更新日期：1984-03-01 00:00:00

abstract：:The quorum-sensing system of Staphylococcus aureus, the accessory gene regulator (Agr) system, is responsible for increased transcription of certain exoprotein genes and decreased transcription of certain cell wall-associated proteins during the postexponential phase of growth. This regulation is important for virulen...

journal_title：Journal of bacteriology

authors： Tseng CW,Zhang S,Stewart GC

更新日期：2004-03-01 00:00:00

abstract：:Mir M7 is a spontaneous morphologically conditional mutant of Klebsiella pneumoniae which grows as round cells (cocci) at pH 7 and as normal rods at pH 5.8. We studied the rates of peptidoglycan synthesis of cocci and rods growing at pH values of 7 and 5.8, respectively. It was found that exponentially growing cocci p...

journal_title：Journal of bacteriology

authors： Satta G,Fontana R,Canepari P,Botta G

更新日期：1979-02-01 00:00:00

abstract：:At some time during sporulation development, cells of Bacillus subtilis develop a commitment to continue sporulation even after addition of or dilution into a fresh nutrient. The extent of commitment was measured by the titer of spores produced at the time at which the original culture sporulated maximally. Since newl...

journal_title：Journal of bacteriology

authors： Cooney PH,Whiteman PF,Freese E

更新日期：1977-02-01 00:00:00

abstract：:The effects of high doses of radiation (1, 5, or 20 Mrad) on the toxicity, pyrogenicity, and immunogenicity of Salmonella typhimurium cells and endotoxin were studied. Toxicity decreased progressively after exposure to 1, 5, or 20 Mrad. The lethal effect of 1-Mrad exposed cells was greater than that of heat-, acetone-...

journal_title：Journal of bacteriology

authors： Previte JJ

更新日期：1968-06-01 00:00:00

abstract：:Escherichia coli sigma70-dependent promoters have typically been characterized as either -10/-35 promoters, which have good matches to both the canonical -10 and the -35 sequences or as extended -10 promoters (TGn/-10 promoters), which have the TGn motif and an excellent match to the -10 consensus sequence. We report ...

journal_title：Journal of bacteriology

authors： Hook-Barnard I,Johnson XB,Hinton DM

更新日期：2006-12-01 00:00:00

abstract：:Hottle, G. A. (Naval Biological Laboratory, University of California, Berkeley), and D. N. Wright. Growth and survival of Mycoplasma neurolyticum in liquid media. J. Bacteriol. 91:1834-1839. 1966.-Maximal growth of Mycoplasma neurolyticum (between 10(8) and 10(9) colony-forming units per ml) was obtained after 3 days ...

journal_title：Journal of bacteriology

authors： Hottle GA,Wright DN

更新日期：1966-05-01 00:00:00

abstract：:Acid phosphatase V of Aspergillus nidulans was purified by ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography. The enzyme demonstrated a charge microheterogeneity on starch and acrylamide gel electrophoresis, but proved to be homogeneous on ultracentrifugation and gel filtration. Phosphata...

journal_title：Journal of bacteriology

authors： Harsanyi Z,Dorn GL

更新日期：1972-04-01 00:00:00