Antigens of Bordetella pertussis. II. Purification of heat-labile toxin.

abstract：:The celA1 gene encoding an endo-beta-1,4-glucanase from a mesophilic actinomycete, strain JM8, identified as Streptomyces halstedii, was cloned and expressed in S. lividans JI66. From the nucleotide sequence of a 1.7-kb DNA fragment we identified an open reading frame of 963 nucleotides encoding a protein of 321 amino...

journal_title：Journal of bacteriology

authors： Fernández-Abalos JM,Sánchez P,Coll PM,Villanueva JR,Pérez P,Santamaría RI

更新日期：1992-10-01 00:00:00

abstract：:The Czc system of Alcaligenes eutrophus mediates resistance to cobalt, zinc, and cadmium through ion efflux catalyzed by the CzcCB2A cation-proton antiporter. DNA sequencing of the region upstream of the czcNICBADRS determinant located on megaplasmid pMOL30 revealed the 5' end of czcN and a gene for a MgtC-like protei...

journal_title：Journal of bacteriology

authors： Grosse C,Grass G,Anton A,Franke S,Santos AN,Lawley B,Brown NL,Nies DH

更新日期：1999-04-01 00:00:00

abstract：:Escherichia coli K antigens (capsular polysaccharides) are divided into two broad classes, designated groups I and II, on the basis of a number of chemical, physical, and genetic criteria. Group I K antigens can be further subdivided on the basis of the absence (group IA) or presence (group IB) of amino sugars in the ...

journal_title：Journal of bacteriology

authors： MacLachlan PR,Keenleyside WJ,Dodgson C,Whitfield C

更新日期：1993-12-01 00:00:00

abstract：:With two-dimensional restriction enzyme analysis we have been able to cleave the Bacillus subtilis genome and resolve the resulting deoxyribonucleic acid (DNA) segments into discrete bands on agarose gels. A general procedure for gene purification has been developed by coupling multidimensional restriction analysis wi...

journal_title：Journal of bacteriology

authors： Potter SS,Bott KF,Newbold JE

更新日期：1977-01-01 00:00:00

abstract：:The prokaryotic transposable element Tn5 has been found to promote the formation of adjacent deletions. The frequency of adjacent deletion formation is much lower than that of normal transposition events. Like normal transposition, however, adjacent deletion formation requires the activity of the transposase protein. ...

journal_title：Journal of bacteriology

authors： Jilk RA,Makris JC,Borchardt L,Reznikoff WS

更新日期：1993-03-01 00:00:00

abstract：:Flavobacterium johnsoniae cells move rapidly over surfaces by gliding motility. Gliding results from the movement of adhesins such as SprB and RemA along the cell surface. These adhesins are delivered to the cell surface by a Bacteroidetes-specific secretion system referred to as the type IX secretion system (T9SS). G...

journal_title：Journal of bacteriology

authors： Shrivastava A,Johnston JJ,van Baaren JM,McBride MJ

更新日期：2013-07-01 00:00:00

abstract：:The production of extracellular enzymes such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt) is activated by the cell density (quorum)-sensing signal, N-(3-oxohexanoyl)-L-homoserine lactone (HSL); plant signals; and aep genes during postexponential growth of Erwinia carotovora subs...

journal_title：Journal of bacteriology

authors： Cui Y,Chatterjee A,Liu Y,Dumenyo CK,Chatterjee AK

更新日期：1995-09-01 00:00:00

abstract：:The distribution of glycerophospholipid-cholesterol acyltransferase in selected bacterial species was examined. Enzyme activity was demonstrated in cell-free growth media from all members of the family Vibrionaceae which were tested except Plesiomonas shigelloides. In each case, enzyme was produced in exponential to e...

journal_title：Journal of bacteriology

authors： MacIntyre S,Trust TJ,Buckley JT

更新日期：1979-07-01 00:00:00

abstract：:In an attempt to isolate the superoxide dismutase (SOD) gene from the anaerobic sulfate-reducing bacterium Desulfoarculus baarsii, a DNA fragment was isolated which functionally complemented an Escherichia coli mutant (sodA sodB) deficient in cytoplasmic SODs. This region carries two open reading frames with sequences...

journal_title：Journal of bacteriology

authors： Pianzzola MJ,Soubes M,Touati D

更新日期：1996-12-01 00:00:00

abstract：:The growth cycle of Rickettsia quintana was studied for the first time in liquid culture. Growth of the microorganism in a transparent broth medium was made possible by the finding that fetal calf serum (FCS), but not calf serum (CS), satisfied the requirement of R. quintana (Fuller strain) for red blood cell lysate. ...

journal_title：Journal of bacteriology

authors： Mason RA

更新日期：1970-07-01 00:00:00

abstract：:In streptomycete anthracycline biosynthetic gene clusters, small open reading frames are located just upstream of minimal polyketide synthase genes. aknX is such a gene found in the aklavinone-aclacinomycin biosynthetic gene cluster of Streptomyces galilaeus. In order to identify its function, the aknX gene was expres...

journal_title：Journal of bacteriology

authors： Chung JY,Fujii I,Harada S,Sankawa U,Ebizuka Y

更新日期：2002-11-01 00:00:00

abstract：:Fimbriae recognizing sialyl galactosides (S fimbriae) were purified from an Escherichia coli strain. The S fimbriae were morphologically identical to type 1 and P fimbriae of E. coli and showed a hemagglutination that was abolished when erythrocytes were treated with neuraminidase. Hemagglutination by the purified fim...

journal_title：Journal of bacteriology

authors： Korhonen TK,Väisänen-Rhen V,Rhen M,Pere A,Parkkinen J,Finne J

更新日期：1984-08-01 00:00:00

abstract：:The brucellae are the etiological agents of brucellosis, a worldwide-distributed zoonosis. These bacteria are facultative intracellular parasites and thus are able to adjust their metabolism to the extra- and intracellular environments encountered during an infectious cycle. However, this aspect of Brucella biology is...

journal_title：Journal of bacteriology

authors： Zúñiga-Ripa A,Barbier T,Conde-Álvarez R,Martínez-Gómez E,Palacios-Chaves L,Gil-Ramírez Y,Grilló MJ,Letesson JJ,Iriarte M,Moriyón I

更新日期：2014-08-15 00:00:00

abstract：:Rogosa, M. (National Institutes of Health, Bethesda, Md.). The genus Veillonella. I. General cultural, ecological, and biochemical considerations. J. Bacteriol. 87:162-170. 1964.-Arguments are presented for excluding Veillonella discoides, V. reniformis, V. orbiculus, and V. vulvovaginitidis from the genus, and for re...

journal_title：Journal of bacteriology

authors： ROGOSA M

更新日期：1964-01-01 00:00:00

abstract：:Purified bulk tRNA from Methanococcus vanielii (carbon source, formate) showed variation in the modified nucleoside pattern reported for Escherichia coli as analyzed by both ion-exchange and thin-layer chromatography. Ribothymidine and 7-methylguanosine were absent; 1-methyladenosine, 1-methylguanosine, N2-methylguano...

journal_title：Journal of bacteriology

authors： Best AN

更新日期：1978-01-01 00:00:00

abstract：:A mutant of Rhizobium meliloti, 4R3, which is unable to grow on aspartate has been isolated. The defect is specific to aspartate utilization, since 4R3 is not an auxotroph and grows as well as its parent strain on other carbon and nitrogen sources. The defect was correlated with an inability to fix nitrogen within nod...

journal_title：Journal of bacteriology

authors： Rastogi VK,Watson RJ

更新日期：1991-05-01 00:00:00

abstract：:Uranyl nitrate inhibited the transport of several amino acids, a vitamin, and a disaccharide into yeast. ...

journal_title：Journal of bacteriology

authors： Maxwell WA,Metzler R,Spoerl E

更新日期：1971-03-01 00:00:00

abstract：:Four genes that encode the homologues of plant geranylgeranyl reductase were isolated from a hyperthermophilic archaeon Archaeoglobus fulgidus, which produces menaquinone with a fully saturated heptaprenyl side chain, menaquinone-7(14H). The recombinant expression of one of the homologues in Escherichia coli led to a ...

journal_title：Journal of bacteriology

authors： Hemmi H,Takahashi Y,Shibuya K,Nakayama T,Nishino T

更新日期：2005-03-01 00:00:00

abstract：:Disruption of gene HI0894 or HI0895 in Haemophilus influenzae Rd, homologs of Escherichia coli acrAB multidrug efflux genes, caused hypersusceptibility to erythromycin, rifampin, novobiocin, and dyes such as ethidium bromide and crystal violet and increased accumulation of radioactive erythromycin, showing that these ...

journal_title：Journal of bacteriology

authors： Sánchez L,Pan W,Viñas M,Nikaido H

更新日期：1997-11-01 00:00:00

abstract：:The expression of R-factor-mediated resistance to tetracycline has been compared in Proteus mirabilis and Escherichia coli. Resistance to a range of concentrations of tetracycline was significantly lower in P. mirabilis than in E. coli in both induced and repressed states. Indirect evidence showed that conditions whic...

journal_title：Journal of bacteriology

authors： Franklin TJ,Rownd R

更新日期：1973-07-01 00:00:00

abstract：:We describe the identification and characterization of two genes and their gene products responsible for determining receptor binding and pilus length in type 1-piliated Escherichia coli. One gene, pilE, conferred the ability of piliated cells to agglutinate guinea pig erythrocytes. The other gene, pilF, determined pi...

journal_title：Journal of bacteriology

authors： Maurer L,Orndorff PE

更新日期：1987-02-01 00:00:00

abstract：:A nitrate-utilizing strain of marine bacteria was isolated in which luciferase was inducible by l-arginine. The induction was highly specific; structural analogues of arginine were ineffective, as were other natural amino acids. Several metabolites of arginine acted as weak inducers but did not affect the rate of indu...

journal_title：Journal of bacteriology

authors： Coffey JJ

更新日期：1967-11-01 00:00:00

abstract：:Bacteriocin 28b from Serratia marcescens binds to Escherichia coli outer membrane proteins OmpA and OmpF and to lipopolysaccharide (LPS) core (J. Enfedaque, S. Ferrer, J. F. Guasch, J. Tomás, and M. Requé, Can. J. Microbiol. 42:19-26, 1996). A cosmid-based genomic library of S. marcescens was introduced into E. coli N...

journal_title：Journal of bacteriology

authors： Guasch JF,Piqué N,Climent N,Ferrer S,Merino S,Rubires X,Tomas JM,Regué M

更新日期：1996-10-01 00:00:00

abstract：:Mutations in the araC gene of Escherichia coli B/r were isolated which alter both activation of the araBAD operon expression and autoregulation. The mutations were isolated on an araC-containing plasmid by hydroxylamine mutagenesis of plasmid DNA. The mutant phenotype selected was the inability to autoregulate. The DN...

journal_title：Journal of bacteriology

authors： Cass LG,Wilcox G

更新日期：1986-06-01 00:00:00

abstract：:The physiological changes in an F1-ATPase-defective mutant of Escherichia coli W1485 growing in a glucose-limited chemostat included a decreased growth yield (60%) and increased specific rates of both glucose consumption (168%) and respiration (171%). Flux analysis revealed that the mutant showed approximately twice a...

journal_title：Journal of bacteriology

authors： Noda S,Takezawa Y,Mizutani T,Asakura T,Nishiumi E,Onoe K,Wada M,Tomita F,Matsushita K,Yokota A

更新日期：2006-10-01 00:00:00

abstract：:Proteus mirabilis swarming behavior is characterized by the development of concentric rings of growth that are formed as cyclic events of swarmer cell differentiation, swarming migration, and cellular differentiation are repeated during colony translocation across a surface. This cycle produces the bull's-eye colony o...

journal_title：Journal of bacteriology

authors： Belas R,Schneider R,Melch M

更新日期：1998-12-01 00:00:00

abstract：:Unlike mutations to streptomycin resistance, which restrict the function of an SU II amber suppressor, mutations to spectinomycin resistance have no restrictive effect. Furthermore, mutations to spectinomycin resistance counteract the restrictive action of streptomycin-resistant mutations when combined in a single str...

journal_title：Journal of bacteriology

authors： Kuwano M,Endo H,Ohnishi Y

更新日期：1969-02-01 00:00:00

abstract：:Aeromonas hydrophila AH-3 lateral flagella are not assembled when bacteria grow in liquid media; however, lateral flagellar genes are transcribed. Our results indicate that A. hydrophila lateral flagellar genes are transcribed at three levels (class I to III genes) and share some similarities with, but have many impor...

journal_title：Journal of bacteriology

authors： Wilhelms M,Gonzalez V,Tomás JM,Merino S

更新日期：2013-04-01 00:00:00

abstract：:DNA from the STA1 (extracellular glucoamylase) gene of Saccharomyces diastaticus was used as a probe to enable the cloning by colony hybridization of three DNA fragments from Saccharomyces cerevisiae; these were designated S1, S2, and SGA (intracellular, sporulation-specific glucoamylase gene). To examine the evolutio...

journal_title：Journal of bacteriology

authors： Yamashita I,Nakamura M,Fukui S

更新日期：1987-05-01 00:00:00

abstract：:Glycogen accumulation in Escherichia coli is inversely related to the growth rate and occurs most actively when cells enter the stationary phase. The levels of the three biosynthetic enzymes undergo corresponding changes under these conditions, suggesting that genetic control of enzyme biosynthesis may account for at ...

journal_title：Journal of bacteriology

authors： Romeo T,Preiss J

更新日期：1989-05-01 00:00:00