Cloning and characterization of two Serratia marcescens genes involved in core lipopolysaccharide biosynthesis.

abstract：:A polyacrylamide gel electrophoretic method was used for identification of L-forms in the genera Streptococcus, Staphylococcus, and Proteus, by comparing phenol-acetic acid-water extracts of homologous parent L-form pairs to one another and to other pairs. The method requires only milligram quantities of material for ...

journal_title：Journal of bacteriology

authors： King JR,Theodore TS,Cole RM

更新日期：1969-10-01 00:00:00

abstract：:A novel phenotype is described for Escherichia coli K-12 carrying the prlA4 allele determining a membrane component of the protein export mechanism. It is manifest as transformation deficiency for plasmids containing the cloned group C streptococcal streptokinase gene, skc. Streptokinase plasmid mutations relieving th...

journal_title：Journal of bacteriology

authors： Müller J,Reinert H,Malke H

更新日期：1989-04-01 00:00:00

abstract：:Polyamines are present in high concentrations in archaea, yet little is known about their synthesis, except by extrapolation from bacterial and eucaryal systems. S-Adenosylmethionine (AdoMet) decarboxylase, a pyruvoyl group-containing enzyme that is required for spermidine biosynthesis, has been previously identified ...

journal_title：Journal of bacteriology

authors： Kim AD,Graham DE,Seeholzer SH,Markham GD

更新日期：2000-12-01 00:00:00

abstract：:We examined 14 multiresistant and 8 ampicillin- or tetracycline-resistant Haemophilus influenzae isolates and 4 ampicillin-resistant H. parainfluenzae isolates for plasmid deoxyribonucleic acid. Sixteen strains carried plasmids. Both "plasmid-free" and plasmid-carrying isolates transferred the antibiotic resistance by...

journal_title：Journal of bacteriology

authors： Roberts MC,Smith AL

更新日期：1980-10-01 00:00:00

abstract：:d-Desthiobiotin synthetase, an enzyme that catalyzes the synthesis of d-desthiobiotin from dl-7,8-diaminopelargonic acid and HCO(3) (-), was purified 100-fold from cells of a biotin mutant strain of Escherichia coli. Adenosine triphosphate and Mg(2+) were shown, especially in purified extracts, to be obligatory for en...

journal_title：Journal of bacteriology

authors： Cheeseman P,Pai CH

更新日期：1970-11-01 00:00:00

abstract：:Streptococcus agalactiae cultures possess an aerobic pathway for glucose oxidation that is strongly inhibited by cyanide. The products of glucose oxidation by aerobically grown cells of S. agalactiae 50 are lactic and acetic acids, acetylmethylcarbinol, and carbon dioxide. Glucose degradation products by aerobically g...

journal_title：Journal of bacteriology

authors： Mickelson MN

更新日期：1967-07-01 00:00:00

abstract：:Two clindamycin-erythromycin resistance transfer factors of Bacteroides fragilis, pBF4 and pBFTM10, were analyzed for regions of DNA homology. Although the plasmids were derived from different clinical isolates of B. fragilis and have different sizes, they showed homology in the clindamycin-erythromycin resistance reg...

journal_title：Journal of bacteriology

authors： Shimell MJ,Smith CJ,Tally FP,Macrina FL,Malamy MH

更新日期：1982-11-01 00:00:00

abstract：:Ben-Hamida, Fakher (Washington University School of Medicine, St. Louis, Mo.), and David Schlessinger. Stability of beta-galactosidase messenger ribonucleic acid in Escherichia coli. J. Bacteriol. 90:1611-1616. 1965.-Synthesis of beta-galactosidase stops within several minutes when preinduced, permeaseless cultures ar...

journal_title：Journal of bacteriology

authors： Ben-Hamida F,Schlessinger D

更新日期：1965-12-01 00:00:00

abstract：:Schlissel, Harvey J. (The University of Kansas, Lawrence), and C. P. Sword. Conditions affecting transformation of a group H streptococcus. J. Bacteriol. 92:1357-1363. 1966.-A defined transforming medium (DTM) containing buffer and 5 to 10 mug per ml of deoxyribonucleic acid was developed to study the physical and che...

journal_title：Journal of bacteriology

authors： Schlissel HJ,Sword CP

更新日期：1966-11-01 00:00:00

abstract：:We demonstrate that expression of the UGA1, CAN1, GAP1, PUT1, PUT2, PUT4, and DAL4 genes is sensitive to nitrogen catabolite repression. The expression of all these genes, with the exception of UGA1 and PUT2, also required a functional GLN3 protein. In addition, GLN3 protein was required for expression of the DAL1, DA...

journal_title：Journal of bacteriology

authors： Daugherty JR,Rai R,el Berry HM,Cooper TG

更新日期：1993-01-01 00:00:00

abstract：:The gap-2 gene, encoding the NAD(P)-dependent D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH2) of the cyanobacterium Synechocystis sp. strain PCC 6803, was cloned by functional complementation of an Escherichia coli gap mutant with a genomic DNA library; this is the first time that this cloning strategy has been us...

journal_title：Journal of bacteriology

authors： Valverde F,Losada M,Serrano A

更新日期：1997-07-01 00:00:00

abstract：:The Mg-2+-Sarkosyl crystals (M band) procedure was used to study the effect of ribonuclease (RNase) A on the association of Escherichia coli deoxyribonucleic acid (DNA) with membrane. Incubation of gently prepared cell extracts with RNase results in the release of DNA from membrane. This effect appears to result from ...

journal_title：Journal of bacteriology

authors： McIntosh MA,Earhart CF

更新日期：1975-05-01 00:00:00

abstract：:Using a system of chromatography through columns of DEAE-Bio-Gel, HTP-Bio-Gel, and CM-Bio-Gel, we isolated and characterized six different (1 leads to 3)-beta-glucanases from cell wall autolysates and cell extracts of Saccharomyces cerevisiae haploid strain 2180B. These enzymes were designated glucanases I, II, IIIA, ...

journal_title：Journal of bacteriology

authors： Hien NH,Fleet GH

更新日期：1983-12-01 00:00:00

abstract：:The isolation of a new thermophilic bacterium, Thermus aquaticus gen. n. and sp. n., is described. Successful enrichment requires incubation at 70 to 75 C, and the use of nutrient media relatively dilute with respect to the organic components. Strains of T. aquaticus have been isolated from a variety of thermal spring...

journal_title：Journal of bacteriology

authors： Brock TD,Freeze H

更新日期：1969-04-01 00:00:00

abstract：:Chorpenning, Frank W. (The Ohio State University, Columbus), and Matthew C. Dodd. Heterogenetic antigens of gram-positive bacteria. J. Bacteriol. 91:1440-1445. 1966.-Soluble antigens obtained by various methods from gram-positive bacteria were used to modify erythrocytes whose hemagglutinating reactions with immune ra...

journal_title：Journal of bacteriology

authors： Chorpenning FW,Dodd MC

更新日期：1966-04-01 00:00:00

abstract：:We describe the isolation and characterization of amber mutations in the lexA gene of Escherichia coli K-12. These mutations, designated spr(Am), were isolated and characterized in a lexA tif sfi genetic background. They abolished the sensitivity of the strain to UV light and resulted in high rates of synthesis of rec...

journal_title：Journal of bacteriology

authors： Pacelli LZ,Edmiston SH,Mount DW

更新日期：1979-01-01 00:00:00

abstract：:In the acetoclastic methanogen Methanothrix soehngenii, acetate is activated to acetyl coenzyme A by acetyl coenzyme A synthetase (Acs). The acs gene, coding for the single Acs subunit, was isolated from a genomic library of M. soehngenii DNA in Escherichia coli by using antiserum raised against the purified Acs. Afte...

journal_title：Journal of bacteriology

authors： Eggen RI,Geerling AC,Boshoven AB,de Vos WM

更新日期：1991-10-01 00:00:00

abstract：:A total of 12 VanA-type vancomycin-resistant enterococci, consisting of 10 Enterococcus faecium isolates and two Enterococcus avium isolates, were examined in detail. The vancomycin resistance conjugative plasmids pHTalpha (65.9 kbp), pHTbeta (63.7 kbp), and pHTgamma (66.5 kbp) were isolated from each of three differe...

journal_title：Journal of bacteriology

authors： Tomita H,Tanimoto K,Hayakawa S,Morinaga K,Ezaki K,Oshima H,Ike Y

更新日期：2003-12-01 00:00:00

abstract：:Vibrio cholerae is the etiologic agent of cholera in humans. Intestinal colonization occurs in a stepwise fashion, initiating with attachment to the small intestinal epithelium. This attachment is followed by expression of the toxin-coregulated pilus, microcolony formation, and cholera toxin (CT) production. We have r...

journal_title：Journal of bacteriology

authors： Jude BA,Martinez RM,Skorupski K,Taylor RK

更新日期：2009-11-01 00:00:00

abstract：:The ethanol oxidase respiratory chain of Gluconobacter suboxydan was characterized by using G. suboxydans subsp. alpha, a variant species of G. suboxydans incapable of oxidizing ethanol. The membranes of G. suboxydans subsp. alpha exhibited neither alcohol dehydrogenase, ethanol oxidase, nor glucose-ferricyanide oxido...

journal_title：Journal of bacteriology

authors： Matsushita K,Nagatani Y,Shinagawa E,Adachi O,Ameyama M

更新日期：1991-06-01 00:00:00

abstract：:This work describes the draft genome sequence of Lactococcus garvieae DCC43. The 2.2-Mb draft genome contains 2,227 predicted protein-coding genes, among which is a region encoding the bacteriocin garvicin ML. No antibiotic resistance genes or capsule-related virulence genes were identified. Two plasmid replication re...

journal_title：Journal of bacteriology

authors： Gabrielsen C,Brede DA,Hernández PE,Nes IF,Diep DB

更新日期：2012-12-01 00:00:00

abstract：:Aspergillus fumigatus secretes a number of glycosidases into the culture medium when the cells are grown in a mineral salts medium containing guar flour (a galactomannan) as the carbon source. At least some of these glycosidases have been reported to be glycoproteins having N-linked oligosaccharides. In this study, we...

journal_title：Journal of bacteriology

authors： Elbein AD,Mitchell M,Molyneux RJ

更新日期：1984-10-01 00:00:00

abstract：:The physical arrangement of cell envelope components leads to the exposure of selected structural motifs which in turn may influence host-parasite interactions. To gain insight into the exposed epitopes, the present study describes a flow cytometric method designed to probe defined molecules on dispersed mycobacteria....

journal_title：Journal of bacteriology

authors： Ozanne V,Ortalo-Magne A,Vercellone A,Fournie JJ,Daffe M

更新日期：1996-12-01 00:00:00

abstract：:Genetic transformation of auxotrophs of the extreme thermophile Thermus thermophilus HB27 to prototrophy was obtained at high frequencies of 10(-2) to 10(-1) when proliferating cell populations were exposed to chromosomal DNA from a nutritionally independent wild-type strain. The transformation frequency was proportio...

journal_title：Journal of bacteriology

authors： Koyama Y,Hoshino T,Tomizuka N,Furukawa K

更新日期：1986-04-01 00:00:00

abstract：:Sulfate-utilizing revertants of a cysteine auxotroph of Salmonella pullorum have been found that cause an accumulation in growth medium of a sulfur compound more oxidized than sulfide. The sulfur compound was presumptively identified as sulfite by the formation of a colored complex in the presence of basic fuchsin and...

journal_title：Journal of bacteriology

authors： Kline BC,Schoenhard DE

更新日期：1969-10-01 00:00:00

abstract：:We have isolated the Bradyrhizobium japonicum gene encoding glutamine synthetase I (glnA) from a phage lambda library by using a fragment of the Escherichia coli glnA gene as a hybridization probe. The rhizobial glnA gene has homology to the E. coli glnA gene throughout the entire length of the gene and can complement...

journal_title：Journal of bacteriology

authors： Carlson TA,Guerinot ML,Chelm BK

更新日期：1985-05-01 00:00:00

abstract：:Pulse labeling of deoxyribonucleic acid (DNA) and radioautography have been used to study the effect of growth rate on nuclear replication in Aspergillus nidulans. When conidia were germinated in media supporting a fast growth rate, the radioactive pulse labeled either all of the nuclei in a cell or none of them. At s...

journal_title：Journal of bacteriology

authors： Kessel M,Rosenberger RF

更新日期：1968-06-01 00:00:00

abstract：:Garrett et al. (Mol. Gen. Genet. 182:326-331, 1981) constructed strains of Escherichia coli harboring derivatives of plasmid pBR322 that carry the lysis genes (S, R, and Rz) of phage lambda. The plasmid construction placed the genes under control of the lactose operon operator-promotor (and thus of lac repressor). Ind...

journal_title：Journal of bacteriology

authors： Crabtree S,Cronan JE Jr

更新日期：1984-04-01 00:00:00

abstract：:The raffinose (raf) operon is negatively controlled by the specific binding of raf repressor (rafR gene) to raf operator (rafO) DNA. Both rafR and rafO have been sequenced. The 1,011-base-pair rafR gene encodes a 336-amino-acid polypeptide containing an N-terminal helix-turn-helix motif. rafO, as defined by in vivo ti...

journal_title：Journal of bacteriology

authors： Aslanidis C,Schmitt R

更新日期：1990-04-01 00:00:00

abstract：:The bacterial peptidoglycan consists of glycan chains of repeating beta-1,4-linked N-acetylglucosaminyl-N-acetylmuramyl units cross-linked through short peptide chains. The polymerization of the glycans, or glycosyltransfer (GT), and transpeptidation (TP) are catalyzed by bifunctional penicillin-binding proteins (PBPs...

journal_title：Journal of bacteriology

authors： Di Guilmi AM,Dessen A,Dideberg O,Vernet T

更新日期：2003-08-01 00:00:00