Partial purification and properties of D-desthiobiotin synthetase from Escherichia coli.

abstract：:Escherichia coli strains mutant in the starvation gene cstC grow normally in a mineral salts medium but are impaired in utilizing amino acids as nitrogen sources. They are also compromised in starvation survival, where amino acid catabolism is important. The cstC gene encodes a 406-amino-acid protein that closely rese...

journal_title：Journal of bacteriology

authors： Fraley CD,Kim JH,McCann MP,Matin A

更新日期：1998-08-01 00:00:00

abstract：:With the exception of Escherichia coli lysyl-tRNA synthetase, the genes coding for the different aminoacyl-tRNA synthetases in procaryotes are always unique. Here we report on the occurrence and cloning of two genes (thrSv and thrS2), both encoding functional threonyl-tRNA synthetase in Bacillus subtilis. The two prot...

journal_title：Journal of bacteriology

authors： Putzer H,Brakhage AA,Grunberg-Manago M

更新日期：1990-08-01 00:00:00

abstract：:Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of convertin...

journal_title：Journal of bacteriology

authors： Römermann D,Friedrich B

更新日期：1985-05-01 00:00:00

abstract：:Growth of Streptococcus faecalis in the presence of l-malate resulted in the induction of a "malic enzyme" [l-malate:nicotinamide adenine dinucleotide (NAD) oxidoreductase (decarboxylating), E.C. 1.1.1.39]. Synthesis of the malic enzyme did not appear to be subject to catabolite repression by intermediate products of ...

journal_title：Journal of bacteriology

authors： London J,Meyer EY

更新日期：1969-05-01 00:00:00

abstract：:Previous work has shown that lethal heat treatment of Bacillus subtilis spores lacking the major DNA-binding proteins SASP-alpha and -beta (alpha-beta- spores) causes significant DNA damage, including many single-strand breaks. In this work we have used a reagent specific for aldehydes present in abasic sites in DNA t...

journal_title：Journal of bacteriology

authors： Setlow B,Setlow P

更新日期：1994-04-01 00:00:00

abstract：:A mutant sensitive to colicin M at 30 degrees C and tolerant at 42 degrees C to high concentrations of colicin M was isolated from Escherichia coli K-12. A temperature shift from 30 to 42 degrees C rescued all cells up to the time they started to lyse at 30 degrees C (25 min after addition of colicin M). The growth ra...

journal_title：Journal of bacteriology

authors： Schaller K,Krauel A,Braun V

更新日期：1981-07-01 00:00:00

abstract：:In this study, we demonstrated the remarkable genome plasticity of lytic lactococcal phages that allows them to rapidly adapt to the dynamic dairy environment. The lytic double-stranded DNA phage ul36 was used to sequentially infect a wild-type strain of Lactococcus lactis and two isogenic derivatives with genes encod...

journal_title：Journal of bacteriology

authors： Labrie SJ,Moineau S

更新日期：2007-02-01 00:00:00

abstract：:The bacterioferritin (BFR) of Escherichia coli K-12 is an iron-storage hemoprotein, previously identified as cytochrome b1. The bacterioferritin gene (bfr) has been cloned, sequenced, and located in the E. coli linkage map. Initially a gene fusion encoding a BFR-lambda hybrid protein (Mr 21,000) was detected by immuno...

journal_title：Journal of bacteriology

authors： Andrews SC,Harrison PM,Guest JR

更新日期：1989-07-01 00:00:00

abstract：:Autotransporter biogenesis is dependent upon BamA, a central component of the β-barrel assembly machinery (BAM) complex. In this report, we detail the role of the other BAM components (BamB-E). We identify the importance of BamD in autotransporter biogenesis and show that BamB, BamC, and BamE are not required. ...

journal_title：Journal of bacteriology

authors： Rossiter AE,Leyton DL,Tveen-Jensen K,Browning DF,Sevastsyanovich Y,Knowles TJ,Nichols KB,Cunningham AF,Overduin M,Schembri MA,Henderson IR

更新日期：2011-08-01 00:00:00

abstract：:Specific growth rates as low as 0.005 hr(-1) (generation times of 20 to 200 hr) of aquatic bacteria in natural waters have been calculated from significant differences between dilution rates and washout rates in a chemostat. The measured growth rates were affected by the treatment of the water samples (type of sterili...

journal_title：Journal of bacteriology

authors： Jannasch HW

更新日期：1969-07-01 00:00:00

abstract：:Formate is an important energy substrate for sulfate-reducing bacteria in natural environments, and both molybdenum- and tungsten-containing formate dehydrogenases have been reported in these organisms. In this work, we studied the effect of both metals on the levels of the three formate dehydrogenases encoded in the ...

journal_title：Journal of bacteriology

authors： da Silva SM,Pimentel C,Valente FM,Rodrigues-Pousada C,Pereira IA

更新日期：2011-06-01 00:00:00

abstract：:Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane...

journal_title：Journal of bacteriology

authors： Gregory MA,Till R,Smith MC

更新日期：2003-09-01 00:00:00

abstract：:Purified bulk tRNA from Methanococcus vanielii (carbon source, formate) showed variation in the modified nucleoside pattern reported for Escherichia coli as analyzed by both ion-exchange and thin-layer chromatography. Ribothymidine and 7-methylguanosine were absent; 1-methyladenosine, 1-methylguanosine, N2-methylguano...

journal_title：Journal of bacteriology

authors： Best AN

更新日期：1978-01-01 00:00:00

abstract：:The phospholipids and lipopolysaccharide of Aeromonas hydrophila were characterized. Phosphatidylethanolamine and phosphatidylglycerol were the major phospholipid components. The outer membrane contained more phosphatidylethanolamine and less phosphatidylglycerol than the inner membrane, and the phospholipids of the o...

journal_title：Journal of bacteriology

authors： Howard SP,Buckley JT

更新日期：1985-01-01 00:00:00

abstract：:Deletion of the citC gene, coding for isocitrate dehydrogenase, arrests sporulation of Bacillus subtilis at stage I after bipolar localization of the cell division protein FtsZ but before formation of the asymmetric septum. A spontaneous extragenic suppressor mutation that overcame the stage I block was found to map w...

journal_title：Journal of bacteriology

authors： Matsuno K,Sonenshein AL

更新日期：1999-06-01 00:00:00

abstract：:Romano, Antonio H. (University of Cincinnati, Cincinnati, Ohio), and Dorothy J. Geason. Pattern of sheath synthesis in Sphaerotilus natans. J. Bacteriol. 88:1145-1150. 1964.-Formation of the characteristic sheath of Sphaerotilus natans was followed by immunofluorescence. Fluorescent antisheath antibody was obtained by...

journal_title：Journal of bacteriology

authors： ROMANO AH,GEASON DJ

更新日期：1964-10-01 00:00:00

abstract：:Using a number of approaches we were unable to demonstrate a chemotactic response of Myxococcus xanthus to a variety of defined and complex materials. These data in addition to a number of prima facie arguments considerably reduce the likelihood that M. xanthus possesses a mechanism for chemotactic behavior. ...

journal_title：Journal of bacteriology

authors： Dworkin M,Eide D

更新日期：1983-04-01 00:00:00

abstract：:Two genes, nahG and nahW, encoding two independent salicylate 1-hydroxylases have been identified in the naphthalene-degrading strain Pseudomonas stutzeri AN10. While nahG resides in the same transcriptional unit as the meta-cleavage pathway genes, forming the naphthalene degradation lower pathway, nahW is situated ou...

journal_title：Journal of bacteriology

authors： Bosch R,Moore ER,García-Valdés E,Pieper DH

更新日期：1999-04-01 00:00:00

abstract：:The gammaproteobacterium Shewanella oneidensis MR-1 utilizes a complex electron transfer network composed primarily of c-type cytochromes to respire under anoxic conditions a variety of compounds, including fumarate, nitrate, and dimethyl sulfoxide (DMSO), in addition to the minerals Fe(III) and Mn(IV). Central to sev...

journal_title：Journal of bacteriology

authors： Cordova CD,Schicklberger MF,Yu Y,Spormann AM

更新日期：2011-05-01 00:00:00

abstract：:The ftsZ gene of Escherichia coli, which lies in a cluster of cell division genes at 2 min on the genetic map, codes for a protein which is thought to play a key role in triggering cell division. Using an ftsZ::lacZ operon fusion, we have studied the transcription of the ftsZ gene under conditions in which cell divisi...

journal_title：Journal of bacteriology

authors： Robin A,Joseleau-Petit D,D'Ari R

更新日期：1990-03-01 00:00:00

abstract：:The homotrimeric enzyme Mt-Cam from Methanosarcina thermophila is the archetype of the gamma class of carbonic anhydrases. A search of databases queried with Mt-Cam revealed that a majority of the homologs comprise a putative subclass (CamH) in which there is major conservation of all of the residues essential for the...

journal_title：Journal of bacteriology

authors： Zimmerman SA,Tomb JF,Ferry JG

更新日期：2010-03-01 00:00:00

abstract：:The DnaA protein concentration in Escherichia coli was increased above the wild-type level by inducing a lacP-controlled dnaA gene located on a plasmid. In these cells with different DnaA protein levels, we measured several parameters: dnaA gene expression; cell size, amount of DNA per cell, and number of origins per ...

journal_title：Journal of bacteriology

authors： Atlung T,Hansen FG

更新日期：1993-10-01 00:00:00

abstract：:Biofilms are structured multicellular communities of bacteria that form through a developmental process. In standing culture, undomesticated strains of Bacillus subtilis produce a floating biofilm, called a pellicle, with a distinct macroscopic architecture. Here we report on a comprehensive analysis of B. subtilis pe...

journal_title：Journal of bacteriology

authors： Kobayashi K

更新日期：2007-07-01 00:00:00

abstract：:Azotobacter vinelandii produces two detectable catalases during growth on minimal medium. The heat-labile catalase expressed during exponential growth phase was identified as a KatG homologue by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a mixed protein sample. The second catalase was heat resista...

journal_title：Journal of bacteriology

authors： Sandercock JR,Page WJ

更新日期：2008-02-01 00:00:00

abstract：:Methanomonas methanooxidans incorporates both carbon atoms of acetate into the glutamate and aspartate families of amino acids during growth on methane; carbon dioxide is also evolved from both carbon atoms of acetate. The distribution of carboxyl-labeled acetate incorporated into convalently bound glutamate is consis...

journal_title：Journal of bacteriology

authors： Wadzinski AM,Ribbons DW

更新日期：1975-07-01 00:00:00

abstract：:Vibrio parahaemolyticus possesses dual flagellar systems adapted for movement under different circumstances. A single polar flagellum propels the bacterium in liquid (i.e., swimming) with a motor that is powered by the sodium motive force. Multiple proton-driven lateral flagella enable translocation over surfaces (i.e...

journal_title：Journal of bacteriology

authors： Stewart BJ,McCarter LL

更新日期：2003-08-01 00:00:00

abstract：:Dienelactone hydrolases have previously been shown to play a crucial role in chlorocatechol degradation via the modified ortho cleavage pathway. Recently, the enzymes induced in 4-fluorobenzoate-utilizing bacteria have been classified into three groups on the basis of their specificity towards cis- and trans-dienelact...

journal_title：Journal of bacteriology

authors： Schlömann M,Ngai KL,Ornston LN,Knackmuss HJ

更新日期：1993-05-01 00:00:00

abstract：:The ESX-1 (ESAT-6 system 1) secretion system plays a conserved role in the virulence of diverse mycobacterial pathogens, including the human pathogen Mycobacterium tuberculosis and M. marinum, an environmental mycobacterial species. The ESX-1 system promotes the secretion of protein virulence factors to the extracytop...

journal_title：Journal of bacteriology

authors： Bosserman RE,Nicholson KR,Champion MM,Champion PA

更新日期：2019-06-21 00:00:00

abstract：:Enterococcus faecalis ATCC 11700 is able to use arginine and the diamine agmatine as a sole energy source. Via the highly homologous deiminase pathways, arginine and agmatine are converted into CO2, NH3, and the end products ornithine and putrescine, respectively. In the arginine deiminase pathway, uptake of arginine ...

journal_title：Journal of bacteriology

authors： Driessen AJ,Smid EJ,Konings WN

更新日期：1988-10-01 00:00:00

abstract：:Thin pili of the closely related IncI1 plasmids ColIb-P9 and R64 are required only for liquid mating and belong to the type IV family of pili. They were sedimented by ultracentrifugation from culture medium in which Escherichia coli cells harboring ColIb-P9- or R64-derived plasmids had been grown, and then the pili we...

journal_title：Journal of bacteriology

authors： Yoshida T,Furuya N,Ishikura M,Isobe T,Haino-Fukushima K,Ogawa T,Komano T

更新日期：1998-06-01 00:00:00