Accumulation of sulfite by a sulfate-using revertant of Salmonella pullorum.

Abstract:

:Sulfate-utilizing revertants of a cysteine auxotroph of Salmonella pullorum have been found that cause an accumulation in growth medium of a sulfur compound more oxidized than sulfide. The sulfur compound was presumptively identified as sulfite by the formation of a colored complex in the presence of basic fuchsin and formaldehyde, oxidation to sulfate by peroxide, and acid-volatility. The acid-volatile material was identified as sulfite by formation of an S-sulfonyl derivative of 5,5'-dithio-bis(2-nitrobenzoic acid) which was chromatographically and electrophoretically identical to an authentic S-sulfonyl derivative. The presence of sulfate in minimal medium is required for sulfite accumulation, and both cysteine and selenate inhibit the accumulation. No evidence was obtained to indicate that a reduced sulfur compound was the precursor of the accumulated sulfite.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kline BC,Schoenhard DE

doi

10.1128/JB.100.1.365-369.1969

subject

Has Abstract

pub_date

1969-10-01 00:00:00

pages

365-9

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

100

pub_type

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