Growth phase-coupled changes of the ribosome profile in natural isolates and laboratory strains of Escherichia coli.

abstract：:The pathway of anaerobic toluene oxidation to benzoyl coenzyme A (benzoyl-CoA) consists of an initial reaction catalyzed by benzylsuccinate synthase, a glycyl radical enzyme adding the methyl group of toluene to the double bond of a fumarate cosubstrate, and a subsequent beta-oxidation pathway of benzylsuccinate. Benz...

journal_title：Journal of bacteriology

authors： Leuthner B,Heider J

更新日期：2000-01-01 00:00:00

abstract：:We report on the characterization of three new transcription units expressed during sporulation in Bacillus subtilis. Two of the units, cse15 and cse60, were mapped at about 123 degrees and 62 degrees on the genetic map, respectively. Their transcription commenced around h 2 of sporulation and showed an absolute requi...

journal_title：Journal of bacteriology

authors： Henriques AO,Bryan EM,Beall BW,Moran CP Jr

更新日期：1997-01-01 00:00:00

abstract：:The production of extracellular enzymes such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt) is activated by the cell density (quorum)-sensing signal, N-(3-oxohexanoyl)-L-homoserine lactone (HSL); plant signals; and aep genes during postexponential growth of Erwinia carotovora subs...

journal_title：Journal of bacteriology

authors： Cui Y,Chatterjee A,Liu Y,Dumenyo CK,Chatterjee AK

更新日期：1995-09-01 00:00:00

abstract：:A class of galactose-resistant mutants has been derived from strains of Salmonella typhimurium which are defective in uridine diphosphoglucose-4-epimerase. Resistant strains are phenotypically similar to parent organisms but do not lyse in the presence of galactose. Low levels of functional epimerase can be detected i...

journal_title：Journal of bacteriology

authors： Shuster CW,Rundell K

更新日期：1969-10-01 00:00:00

abstract：:We present the complete genome sequence of Listeria welshimeri, a nonpathogenic member of the genus Listeria. Listeria welshimeri harbors a circular chromosome of 2,814,130 bp with 2,780 open reading frames. Comparative genomic analysis of chromosomal regions between L. welshimeri, Listeria innocua, and Listeria monoc...

journal_title：Journal of bacteriology

authors： Hain T,Steinweg C,Kuenne CT,Billion A,Ghai R,Chatterjee SS,Domann E,Kärst U,Goesmann A,Bekel T,Bartels D,Kaiser O,Meyer F,Pühler A,Weisshaar B,Wehland J,Liang C,Dandekar T,Lampidis R,Kreft J,Goebel W,Chakraborty

更新日期：2006-11-01 00:00:00

abstract：:We have cloned fragments of DNA (up to 13 kb), from Pseudomonas putida AJ1, that code for two stereospecific haloalkanoate dehalogenases. These enzymes are highly specific for D and L substrates. The two genes, designated hadD and hadL, have been isolated and independently expressed in Escherichia coli and P. putida h...

journal_title：Journal of bacteriology

authors： Barth PT,Bolton L,Thomson JC

更新日期：1992-04-01 00:00:00

abstract：:In this study, we demonstrated the remarkable genome plasticity of lytic lactococcal phages that allows them to rapidly adapt to the dynamic dairy environment. The lytic double-stranded DNA phage ul36 was used to sequentially infect a wild-type strain of Lactococcus lactis and two isogenic derivatives with genes encod...

journal_title：Journal of bacteriology

authors： Labrie SJ,Moineau S

更新日期：2007-02-01 00:00:00

abstract：:In vivo and in vitro methylation, methanol production assays, and the use of specific antibodies raised against the sensory transducing protein Tar in Escherichia coli all failed to demonstrate the presence of methyl-accepting chemotaxis proteins (MCPs) in the photosynthetic bacterium Rhodobacter sphaeroides, although...

journal_title：Journal of bacteriology

authors： Sockett RE,Armitage JP,Evans MC

更新日期：1987-12-01 00:00:00

abstract：:A genetic analysis of a 12-kb DNA fragment containing Pseudomonas denitrificans cob genes was performed by transposon-mediated insertional mutagenesis. The nucleotide sequence and genetic analysis have shown that a 4.8-kb DNA subfragment carried two cob genes (cobS and cobT). Biochemical data concerning the complement...

journal_title：Journal of bacteriology

authors： Cameron B,Guilhot C,Blanche F,Cauchois L,Rouyez MC,Rigault S,Levy-Schil S,Crouzet J

更新日期：1991-10-01 00:00:00

abstract：:Evidence is reported here that alkalinization of Clostridium acetobutylicum cytoplasm involves hydrogenase activity. A decrease of in vivo hydrogenase activity is accompanied by intracellular accumulation of protons leading to a negative (interior acidic) pH gradient. However, the organism is able to maintain a consta...

journal_title：Journal of bacteriology

authors： Girbal L,Vasconcelos I,Soucaille P

更新日期：1994-10-01 00:00:00

abstract：:Bacteroides thetaiotaomicron, a gram-negative obligate anaerobe, appears to utilize starch by first binding the polymer to its surface and then translocating it into the periplasmic space. Several genes that encode enzymes or outer membrane proteins involved in starch utilization have been identified. These have been ...

journal_title：Journal of bacteriology

authors： D'Elia JN,Salyers AA

更新日期：1996-12-01 00:00:00

abstract：:In this study, we demonstrated that the surface-expressed enolase from diarrheal isolate SSU of Aeromonas hydrophila bound to human plasminogen and facilitated the latter's tissue-type plasminogen activator-mediated activation to plasmin. The bacterial surface-bound plasmin was more resistant to the action of its spec...

journal_title：Journal of bacteriology

authors： Sha J,Erova TE,Alyea RA,Wang S,Olano JP,Pancholi V,Chopra AK

更新日期：2009-05-01 00:00:00

abstract：:Antigen 43 has been identified as a unique protein complex in the outer membrane of Escherichia coli. The complex contains two different polypeptides, alpha (Mr, 60,000) and beta (Mr, 53,000), in equal stoichiometry (P. Owen, P. Caffrey, and L.-G. Josefsson, J. Bacteriol. 169:3770-3777, 1987). The alpha subunit was re...

journal_title：Journal of bacteriology

authors： Caffrey P,Owen P

更新日期：1989-07-01 00:00:00

abstract：:Vibrio vulnificus is a causative agent of serious food-borne diseases in humans related to the consumption of raw seafood. It secretes a metalloprotease that is associated with skin lesions and serious hemorrhagic complications. In this study, we purified and characterized an extracellular metalloprotease (designated ...

journal_title：Journal of bacteriology

authors： Chang AK,Kim HY,Park JE,Acharya P,Park IS,Yoon SM,You HJ,Hahm KS,Park JK,Lee JS

更新日期：2005-10-01 00:00:00

abstract：:We identified in Listeria monocytogenes a gene encoding a protein homologous to MecA, a regulatory protein acting with ClpC and ComK in the competence pathway of Bacillus subtilis. In L. monocytogenes, MecA is involved, along with ClpC and ClpP, in the downregulation of a 64-kDa secreted protein. In B. subtilis, the M...

journal_title：Journal of bacteriology

authors： Borezee E,Msadek T,Durant L,Berche P

更新日期：2000-10-01 00:00:00

abstract：:Horizontal acquisition of novel chromosomal genes is considered to be a key process in the evolution of bacterial pathogens. However, the identification of gene presence or absence could be hindered by the inconsistencies in bacterial genome annotations. Here, we performed a cross-annotation of omnipresent core and mo...

journal_title：Journal of bacteriology

authors： Paul S,Sokurenko EV,Chattopadhyay S

更新日期：2016-11-04 00:00:00

abstract：:ClpX is a well-characterized bacterial chaperone that plays a role in many processes, including protein turnover and the remodeling of macromolecular complexes. All of these activities require ATP hydrolysis-dependent, ClpX-mediated protein unfolding. Here we used site-directed mutagenesis in combination with genetics...

journal_title：Journal of bacteriology

authors： Haeusser DP,Lee AH,Weart RB,Levin PA

更新日期：2009-03-01 00:00:00

abstract：:A simple three-step procedure is described which yields electrophoretically homogeneous preparations of ribitol-5-phosphate dehydrogenase and xylitol-5-phosphate dehydrogenase. The former enzyme is a 115,000-molecular-weight protein composed of two subunits of identical size and is specific for its substrate, ribitol....

journal_title：Journal of bacteriology

authors： Hausman SZ,London J

更新日期：1987-04-01 00:00:00

abstract：:The ftsH gene encodes an ATP- and Zn(2+)-dependent metalloprotease which is anchored to the cytoplasmic membrane via two transmembrane segments in such a way that the very short amino- and the long carboxy termini are exposed to the cytoplasm. Deletion of the ftsH gene in Bacillus subtilis results in a pleiotropic phe...

journal_title：Journal of bacteriology

authors： Wehrl W,Niederweis M,Schumann W

更新日期：2000-07-01 00:00:00

abstract：:Preparations of membrane plus wall derived from Bacillus subtilis W23 were used to study the in vitro synthesis of peptidoglycan and teichoic acid and their linkage to the preexisting cell wall. The teichoic acid synthesis showed an ordered requirement for the incorporation of N-acetylglucosamine from uridine 5'-dipho...

journal_title：Journal of bacteriology

authors： Wyke AW,Ward JB

更新日期：1977-06-01 00:00:00

abstract：:The large and small subunits of Escherichia coli glutamate synthase were isolated. The small subunit catalyzes the NH(3)-dependent synthesis of glutamate. The large subunit exhibits glutaminase activity. ...

journal_title：Journal of bacteriology

authors： Mantsala P,Zalkin H

更新日期：1976-04-01 00:00:00

abstract：:A procedure for the isolation of Bacillus subtilis mutants that appear to be defective in septum synthesis has been described. Fourteen mutants isolated with this technique were found to be located at four distinct loci on the B. subtilis chromosome. These have been designated divA, divB, divC, and divD. The four muta...

journal_title：Journal of bacteriology

authors： Van Alstyne D,Simon MI

更新日期：1971-12-01 00:00:00

abstract：:The fermentative hyperthermophile Pyrococcus furiosus contains an NADPH-utilizing, heterotetrameric (alphabetagammadelta), cytoplasmic hydrogenase (hydrogenase I) that catalyzes both H(2) production and the reduction of elemental sulfur to H(2)S. Herein is described the purification of a second enzyme of this type, hy...

journal_title：Journal of bacteriology

authors： Ma K,Weiss R,Adams MW

更新日期：2000-04-01 00:00:00

abstract：:A mutant of Escherichia coli, previously shown to contain abnormal nucleoside triphosphate pools, was found to be defective in its ability to synthesize thymidine nucleotides. The defect is not in the enzyme thymidylate synthetase but in deoxycytidine triphosphate deaminase, an enzyme that supplies deoxyuridine monoph...

journal_title：Journal of bacteriology

authors： O'Donovan GA,Edlin G,Fuchs JA,Neuhard J,Thomassen E

更新日期：1971-02-01 00:00:00

abstract：:Monobromoacetate (MBA) is toxic for the 1,2-dichloroethane-degrading bacterium Xanthobacter autotrophicus GJ10 at concentrations higher than 5 mM. Mutants which are able to grow on higher concentrations of MBA were isolated and found to overexpress haloacid dehalogenase, which is encoded by the dhlB gene. In mutant GJ...

journal_title：Journal of bacteriology

authors： van der Ploeg J,Willemsen M,van Hall G,Janssen DB

更新日期：1995-03-01 00:00:00

abstract：:Plasmid R1 is a low-copy-number plasmid that is present at a level of about four or five copies per average cell. The copy number is controlled posttranscriptionally at the level of synthesis of the rate-limiting initiator protein RepA. In addition to this, R1 has an auxiliary system that derepresses a second promoter...

journal_title：Journal of bacteriology

authors： Olsson JA,Paulsson J,Nordström K

更新日期：2004-01-01 00:00:00

abstract：:Expression of fimA, the structural gene for type 1 fimbriae of Escherichia coli, is phase variable. Significant homologies were identified between the recombinases which control fimbrial phase variation, FimB and FimE, and the integrase class of site-specific recombinases. Normal expression of fimA was shown to requir...

journal_title：Journal of bacteriology

authors： Dorman CJ,Higgins CF

更新日期：1987-08-01 00:00:00

abstract：:Numerous field isolates of Moraxella bovis have previously been classified by serological techniques into seven serogroups, each defined by homologous cross-reaction with antisera prepared against purified pili of a single prototype strain. The gene encoding pilin from each of the prototype strains has been characteri...

journal_title：Journal of bacteriology

authors： Atwell JL,Tennent JM,Lepper AW,Elleman TC

更新日期：1994-08-01 00:00:00

abstract：:Monoubiquitination of the 12-transmembrane segment (12-TMS) Saccharomyces cerevisiae maltose transporter promoted the maximal internalization rate of this protein. This modification is similar to that of the 7-TMS alpha-factor receptor but different from that of the 12-TMS uracil and general amino acid permeases. This...

journal_title：Journal of bacteriology

authors： Lucero P,Peñalver E,Vela L,Lagunas R

更新日期：2000-01-01 00:00:00

abstract：UNLABELLED:The ubiquitous bacterial second messenger cyclic di-GMP (c-di-GMP) has recently become prominent as a trigger for biofilm formation in many bacteria. It is generated by diguanylate cyclases (DGCs; with GGDEF domains) and degraded by specific phosphodiesterases (PDEs; containing either EAL or HD-GYP domains)....

journal_title：Journal of bacteriology

authors： Povolotsky TL,Hengge R

更新日期：2015-08-24 00:00:00