Abstract:
:In this study, we demonstrated the remarkable genome plasticity of lytic lactococcal phages that allows them to rapidly adapt to the dynamic dairy environment. The lytic double-stranded DNA phage ul36 was used to sequentially infect a wild-type strain of Lactococcus lactis and two isogenic derivatives with genes encoding two phage resistance mechanisms, AbiK and AbiT. Four phage mutants resistant to one or both Abi mechanisms were isolated. Comparative analysis of their complete genomes, as well as morphological observations, revealed that phage ul36 extensively evolved by large-scale homologous and nonhomologous recombination events with the inducible prophage present in the host strain. One phage mutant exchanged as much as 79% of its genome compared to the core genome of ul36. Thus, natural phage defense mechanisms and prophage elements found in bacterial chromosomes contribute significantly to the evolution of the lytic phage population.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Labrie SJ,Moineau Sdoi
10.1128/JB.01111-06subject
Has Abstractpub_date
2007-02-01 00:00:00pages
1482-7issue
4eissn
0021-9193issn
1098-5530pii
JB.01111-06journal_volume
189pub_type
杂志文章abstract::Growth of Streptococcus faecalis in the presence of l-malate resulted in the induction of a "malic enzyme" [l-malate:nicotinamide adenine dinucleotide (NAD) oxidoreductase (decarboxylating), E.C. 1.1.1.39]. Synthesis of the malic enzyme did not appear to be subject to catabolite repression by intermediate products of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.2.705-711.1969
更新日期:1969-05-01 00:00:00
abstract::Bacillus subtilis strains containing deletions in the genes coding for one or two of the major small, acid-soluble spore proteins (SASP; termed SASP-alpha and SASP-beta) were constructed. These mutants sporulated normally, but the spores lacked either SASP-alpha, SASP-beta, or both proteins. The level of minor SASP di...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.1.174-178.1986
更新日期:1986-07-01 00:00:00
abstract::A third nuclear protein-coding gene termed PET122 has been shown to be required for a post-transcriptional step in expression of the mitochondrial COX3 gene is Saccharomyces cerevisiae. pet122 mutants fail to produce cytochrome c oxidase subunit III, which is the polypeptide product of the COX3 gene, but produce norma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.3.1399-1402.1988
更新日期:1988-03-01 00:00:00
abstract::Membrane vesicles containing internal alcohol dehydrogenase and nicotinamide adenine dinucleotide (NAD) were prepared from Escherichia coli ML308-225. Upon the addition of ethanol, these vesicles respired and transported several amino acids. Transport rates driven by internally generated reduced NAD (NADH) were about ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.2.861-865.1974
更新日期:1974-11-01 00:00:00
abstract::Analysis of rRNA genes in Vibrio fischeri indicates the presence of eight rRNA gene sets in this organism. It was found that the genes for 5S rRNA, 16S rRNA, and 23S rRNA are organized in operons in the following order: 5' end 16S rRNA 23S RNA 5S rRNA 3' end. Although the operons are homologous, they are not identical...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.2318-2321.1987
更新日期:1987-05-01 00:00:00
abstract::We have developed a rapid and sensitive fluorimetric method, based on the formation of a fluorescent product from nitrosation of 2,3-diaminonaphthalene, for measuring the ability of bacteria to catalyze nitrosation of amines. We have shown in Escherichia coli that nitrosation can be induced under anaerobic conditions ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.359-364.1988
更新日期:1988-01-01 00:00:00
abstract::Genetic transformation of auxotrophs of the extreme thermophile Thermus thermophilus HB27 to prototrophy was obtained at high frequencies of 10(-2) to 10(-1) when proliferating cell populations were exposed to chromosomal DNA from a nutritionally independent wild-type strain. The transformation frequency was proportio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.1.338-340.1986
更新日期:1986-04-01 00:00:00
abstract::A gene required for growth and viability in recA mutants of Escherichia coli K-12 was identified. This gene, rdgB (for Rec-dependent growth), mapped near 64 min on the E. coli genetic map. In a strain carrying a temperature-sensitive recA allele, recA200, and an rdgB mutation, DNA synthesis but not protein synthesis c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.9.4203-4210.1987
更新日期:1987-09-01 00:00:00
abstract::An apparent operator-constitutive mutation was discovered in the repressible acid phosphatase system in Saccharomyces cerevisiae. The site of mutation, designated PHOO, was found to be closely linked to the phoD locus. The mutant allele, PHOO, was semidominant over the wild-type allele and effective for the expression...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.2.608-617.1974
更新日期:1974-11-01 00:00:00
abstract::Glycerol is a primary energy source for heterotrophic haloarchaea and a major component of "salty" biodiesel waste. Glycerol is catabolized solely by glycerol kinase (encoded by glpK) to glycerol-3-phosphate (G3P) in Haloferax volcanii. Here we characterized the next critical step of this metabolic pathway: the conver...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00276-11
更新日期:2011-09-01 00:00:00
abstract::Glyoxal is toxic and mutagenic α-oxoaldehyde generated in vivo as an oxidation by-product of sugar metabolism. We selected glyoxal-resistant mutants from an Escherichia coli strain lacking major glyoxal-detoxifying genes, gloA and yqhD, by growing cells in medium containing a lethal concentration of glyoxal. The mutan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06062-11
更新日期:2012-04-01 00:00:00
abstract::Atractyloside and carboxyatractyloside partially inhibited nitrogenase activity (acetylene reduction) by isolated vesicles of Frankia strain EAN1pec. Extracts of disrupted vesicles showed nitrogenase activity that was not affected by the inhibitors. The vesicles accumulated ATP by an atractyloside-sensitive mechanism....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.7.3053-3057.1988
更新日期:1988-07-01 00:00:00
abstract::Cyclic di-AMP (c-di-AMP) is an essential and ubiquitous second messenger among bacteria. c-di-AMP regulates many cellular pathways through direct binding to several molecular targets in bacterial cells. c-di-AMP depletion is well known to destabilize the bacterial cell wall, resulting in increased bacteriolysis and en...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00307-20
更新日期:2020-11-19 00:00:00
abstract::The structure of sheathed flagella from Bdellovibrio bacteriovorus was investigated. The first three periods of these flagella were characterized by progressively smaller wavelengths and amplitudes in periods more distal to the cell. The damped appearance was due to a single nonrandom transition between two helical st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.3.1038-1046.1985
更新日期:1985-09-01 00:00:00
abstract::A widely distributed family of small regulators, called C proteins, controls a subset of restriction-modification systems. The C proteins studied to date activate transcription of their own genes and that of downstream endonuclease genes; this arrangement appears to delay endonuclease expression relative to that of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.2.488-497.2005
更新日期:2005-01-01 00:00:00
abstract::When incubated in the light under 100% oxygen, wild-type blue-green algae (Anacystis nidulans, Synechococcus cedrorum) die out rapidly at temperatures of 4 to 15 C, and at 35 C (or at 26 C in the case of S. cedrorum) in the absence of CO(2). Photosynthesis is impaired in these cells long before they die. Blocking of p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.3.682-689.1972
更新日期:1972-09-01 00:00:00
abstract::Site-specific mutagenesis has identified amino acids involved in bR proton transport. Biophysical studies of the mutants have elucidated the roles of two membrane-embedded residues: Asp-85 serves as the acceptor for the proton from the isomerized retinylidene Schiff base, and Asp-96 participates in reprotonation of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/jb.175.6.1555-1560.1993
更新日期:1993-03-01 00:00:00
abstract::The effects of temperature and attractants on chemotaxis in psychrotrophic Pseudomonas fluorescens were examined using the Adler capillary assay technique. Several organic acids, amino acids, and uronic acids were shown to be attractants, whereas glucose and its oxidation products, gluconate and 2-ketogluconate, elici...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.1.338-342.1980
更新日期:1980-07-01 00:00:00
abstract::Successful completion of the bacteriophage P2 lytic cycle requires phage-induced lysis of its Escherichia coli host, a process that is poorly understood. Genetic analysis of lysis-deficient mutants defined a single locus, gene K, which lies within the largest late transcription unit of P2 and maps between head gene L ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.16.4974-4984.1994
更新日期:1994-08-01 00:00:00
abstract::Netropsin, a polypeptide antibiotic which binds specifically to adenylate-thymidylate-rich regions of deoxyribonucleic acid, inhibitis sporulation at about stage II, but does not inhibit growth of Bacillys subtilis. An analysis of the sporulation-associated enzymes aconitase, alkaline phosphatase, and glucose dehydrog...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.1.80-85.1976
更新日期:1976-10-01 00:00:00
abstract::Escherichia coli VC30 is a temperature-sensitive mutant which is defective in autolysis. Strain VC30 lyses at 30 degrees C when treated with beta-lactam antibiotics or D-cycloserine or when deprived of diaminiopimelic acid. The same treatments inhibit growth of the mutant at 42 degrees C but do not cause lysis. Strain...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.1.385-387.1984
更新日期:1984-07-01 00:00:00
abstract::In cyanobacteria, gene expression is regulated mainly at the level of transcription initiation, which is mediated by the RNA polymerase holoenzyme. The RNA polymerase core is catalytically active, while the sigma factor recognizes promoter sequences. Group 2 sigma factors are similar to the principal sigma factor but ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00132-09
更新日期:2009-06-01 00:00:00
abstract::We previously described a gene, lpt3, required for the addition of phosphoethanolamine (PEtn) at the 3 position on the beta-chain heptose (HepII) of the inner-core Neisseria meningitidis lipopolysaccharide (LPS), but it has long been recognized that the inner-core LPS of some strains possesses PEtn at the 6 position (...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.20.6970-6982.2004
更新日期:2004-10-01 00:00:00
abstract::Transcription of the dnaA gene of the cyanobacterium Synechocystis sp. strain PCC 6803 is light dependent and yields a monocistronic mRNA, as determined by Northern analysis. Surprisingly, mutants with inactivated dnaA were viable. In batch cultures under standard conditions, the mutants grew like the wild type and di...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.18.4946-4949.1998
更新日期:1998-09-01 00:00:00
abstract::Thin pili of the closely related IncI1 plasmids ColIb-P9 and R64 are required only for liquid mating and belong to the type IV family of pili. They were sedimented by ultracentrifugation from culture medium in which Escherichia coli cells harboring ColIb-P9- or R64-derived plasmids had been grown, and then the pili we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.11.2842-2848.1998
更新日期:1998-06-01 00:00:00
abstract::Developmental expression of the Myxococcus xanthus gene 4521 requires extracellular A-signal. This signal is generated in response to nutrient limitation and functions in cell density sensing. To identify the upstream limit of the minimum region required in vivo for A-signal-dependent 4521 expression, a 5' deletion an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.16.4645-4651.1995
更新日期:1995-08-01 00:00:00
abstract::A cyclic polyol, 1,5-anhydro-D-glucitol (AG), is widely detected in most organisms, although little is known about its metabolism and physiological roles. The present study demonstrates the synthesis of AG in Escherichia coli C600. The major portion of the synthesized AG was indicated to be derived from glucose retain...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.22.7138-7141.1993
更新日期:1993-11-01 00:00:00
abstract::Pseudomonas putida DOT-T1 was isolated after enrichment on minimal medium with 1% (vol/vol) toluene as the sole C source. The strain was able to grow in the presence of 90% (vol/vol) toluene and was tolerant to organic solvents whose log P(ow) (octanol/water partition coefficient) was higher than 2.3. Solvent toleranc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.14.3911-3916.1995
更新日期:1995-07-01 00:00:00
abstract::Using a number of approaches we were unable to demonstrate a chemotactic response of Myxococcus xanthus to a variety of defined and complex materials. These data in addition to a number of prima facie arguments considerably reduce the likelihood that M. xanthus possesses a mechanism for chemotactic behavior. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.1.437-442.1983
更新日期:1983-04-01 00:00:00
abstract::Escherichia coli K antigens (capsular polysaccharides) are divided into two broad classes, designated groups I and II, on the basis of a number of chemical, physical, and genetic criteria. Group I K antigens can be further subdivided on the basis of the absence (group IA) or presence (group IB) of amino sugars in the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.23.7515-7522.1993
更新日期:1993-12-01 00:00:00