Abstract:
:A procedure for the isolation of Bacillus subtilis mutants that appear to be defective in septum synthesis has been described. Fourteen mutants isolated with this technique were found to be located at four distinct loci on the B. subtilis chromosome. These have been designated divA, divB, divC, and divD. The four mutants in the divA group synthesize septa; however, they do so with a high frequency of error resulting in minicell production. Mapping data were obtained by scoring cotransduction frequencies using PBS1-transducing lysates. Thus, some of the genes apparently involved in septum synthesis and their order on the genome have been established. The results of a study by electron microscope of some of the mutants is also presented.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Van Alstyne D,Simon MIdoi
10.1128/JB.108.3.1366-1379.1971subject
Has Abstractpub_date
1971-12-01 00:00:00pages
1366-79issue
3eissn
0021-9193issn
1098-5530journal_volume
108pub_type
杂志文章abstract::Harper, M. K. (U.S. Department of Agriculture, Washington, D.C.), H. R. Curran, and M. J. Pallansch. Properties of electrodialyzed bacterial spores. J. Bacteriol. 88:1338-1340. 1964.-Washed spores of Bacillus cereus, B. megaterium, and B. stearothermophilis suspended in distilled water were electrodialyzed at a potent...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.5.1338-1340.1964
更新日期:1964-11-01 00:00:00
abstract::The Bacillus subtilis ResD-ResE two-component regulatory system activates genes involved in nitrate respiration in response to oxygen limitation or nitric oxide (NO). The sensor kinase ResE activates the response regulator ResD through phosphorylation, which then binds to the regulatory region of genes involved in ana...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.6.1694-1704.2004
更新日期:2004-03-01 00:00:00
abstract::Cholera, a severe diarrheal disease, is caused by ingestion of the gram-negative bacterium Vibrio cholerae. Expression of V. cholerae virulence factors is highly regulated at the transcriptional and posttranscriptional levels by a complex network of proteins and small noncoding RNAs. The direct activator of transcript...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00512-08
更新日期:2008-12-01 00:00:00
abstract::The carbon monoxide (CO) dehydrogenase activity band from Clostridium pasteurianum was shown to contain nickel by in situ activity staining of polyacrylamide gels. However, the majority of the nickel in cell extracts was found to electrophorese independently of CO dehydrogenase. Comparative analysis with Clostridium t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.561-566.1982
更新日期:1982-02-01 00:00:00
abstract::A hybrid plasmid was constructed between the 5.3-megadalton plasmid (pUH24) of Anacystis nidulans R2 and the Escherichia coli plasmid pBR322. This was accomplished by adding a transposon to pBR322 and transforming this DNA into A. nidulans. One resultant hybrid, pLS103, had a molecular weight of 6.8 x 10(6), replicate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.1.410-413.1982
更新日期:1982-04-01 00:00:00
abstract::In vivo assembly of the Klebsiella aerogenes urease nickel metallocenter requires the presence of UreD, UreF, and UreG accessory proteins and is further facilitated by UreE. Prior studies had shown that urease apoprotein exists in an uncomplexed form as well as in a series of UreD-urease (I.-S. Park, M.B. Carr, and R....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.18.5417-5421.1996
更新日期:1996-09-01 00:00:00
abstract::The levels of transfer ribonucleic acids (tRNAs) specific for 14 amino acids were almost identical in dormant spores and in spores germinated from 6 to 75 min. Germinated spore tRNAs specific for all amino acids tested were between 63 and 93% charged, and there was no significant change in this value from 6 to 75 min ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.3.1067-1074.1974
更新日期:1974-06-01 00:00:00
abstract::Transcription of the virB gene, a transcriptional regulator of invasion genes on the large plasmid of Shigella flexneri, is strictly regulated by growth temperature; when bacteria are grown at 37 degrees C, virB transcription is highly activated, while at 30 degrees C the level of virB transcription decreases to less ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.4.1094-1097.1995
更新日期:1995-02-01 00:00:00
abstract::Five lacY mutants with amber stop codons at known positions were each placed into 12 different suppressor strains. The 60 amino acid substitutions obtained in this manner were tested for growth on lactose-minimal medium plates and for transport of lactose, melibiose, and thiomethylgalactoside. Most of the amino acid s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.16.5436-5441.1992
更新日期:1992-08-01 00:00:00
abstract::SecB is a cytosolic protein required for rapid and efficient export of particular periplasmic and outer membrane proteins in Escherichia coli. SecB promotes export by stabilizing newly synthesized precursor proteins in a nonnative conformation and by targeting the precursors to the inner membrane. Biochemical studies ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.10.3010-3017.1999
更新日期:1999-05-01 00:00:00
abstract::Tn2603 is a multiple-resistance transposon encoding resistance to ampicillin, streptomycin, sulfonamide, and mercury and having a molecular size of 20 kilobase pairs, with 200-base-pair inverted repeats at both ends. The essential sites and functions of Tn2603 which are required for its transposition were determined t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.2.723-728.1982
更新日期:1982-08-01 00:00:00
abstract::Most iron in mammals is found within the heme prosthetic group. Consequently, many bacterial pathogens possess heme acquisition systems to utilize iron from the host. Here, we demonstrate that Mycobacterium tuberculosis can utilize heme as an iron source, suggesting that M. tuberculosis possesses a yet-unknown heme ac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01312-10
更新日期:2011-04-01 00:00:00
abstract::Salmonella enteritidis was injected intravenously, intraperitoneally, or subcutaneously into specific pathogen-free mice. The number of organisms in the blood, liver, spleen, peritoneal cavity, and draining inguinal lymph node was determined by daily enumeration. Opsonization of the organism with hyperimmune serum inc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.2.667-675.1969
更新日期:1969-02-01 00:00:00
abstract::Type 1 pili of Escherichia coli are the prototype of the somatic class of pili found on many strains of bacteria. As a first step in the genetic analysis of type 1 piliation, an extensive series of nonpiliated derivatives of E. coli K-12 strain AW405, was characterized to produce attached or free pili when examined in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.1.495-505.1977
更新日期:1977-04-01 00:00:00
abstract::In growing cultures of Escherichia coli, the nonacylated glycerol of phosphatidylglycerol (PG) is labeled more rapidly than is the acylated glycerol. This is, in part, due to a rapid exchange reaction of the nonacylated glycerol. Only some of the PG molecules undergo this reaction while others are stable. Using a muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.1.210-214.1973
更新日期:1973-10-01 00:00:00
abstract::Three floc-forming, gram-negative, polarly flagellated rods were isolated and characterized. Our isolates were compared to four similar floc-forming organisms previously isolated in another laboratory and classified as two species of Zoogloea, one of Pseudomonas, and as one unidentified gram-negative rod. Possession o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.5.1903-1909.1968
更新日期:1968-05-01 00:00:00
abstract::To study growth and cell division of anaerobic hyperthermophilic archaea in vivo, a cultivation technique using glass capillaries was developed. At temperatures of 90 to 98 degrees C, at least 10 successive cell divisions of Pyrodictium abyssi TAG 11 were documented. Cells divide by binary fission. Visualized under a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.16.5114-5118.1999
更新日期:1999-08-01 00:00:00
abstract::Benzoyl coenzyme A (benzoyl-CoA) reductase is a key enzyme in the anaerobic metabolism of aromatic compounds catalyzing the ATP-driven reductive dearomatization of benzoyl-CoA. The enzyme from Thauera aromatica uses a reduced 2[4Fe-4S] ferredoxin as electron donor. In this work, we identified 2-oxoglutarate:ferredoxin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.14.3975-3983.2002
更新日期:2002-07-01 00:00:00
abstract::Highly efficient promoters of coliphage T5 were identified by selecting for functional properties. Eleven such promoters belonging to all three expression classes of the phage were analyzed. Their average AT content was 75% and reached 83% in subregions of the sequences. Besides the well-known conserved sequences arou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.1.70-77.1985
更新日期:1985-10-01 00:00:00
abstract::A clinical isolate of Staphylococcus aureus was found to be tolerant (MBC much greater than MIC) to a number of beta-lactam antibiotics, including oxacillin. Biophotometric analysis showed that a number of concentrations of oxacillin were capable of stimulating rapid cellular lysis in this organism, but the extent of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1831-1836.1988
更新日期:1988-04-01 00:00:00
abstract::Motility in mycobacteria was described for the first time in 1999. It was reported that Mycobacterium smegmatis and Mycobacterium avium could spread on the surface of solid growth medium by a sliding mechanism and that the presence of cell wall glycopeptidolipids was essential for motility. We recently reported that M...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00572-08
更新日期:2008-10-01 00:00:00
abstract::Mycoplasma mobile has a unique mechanism that enables it to glide on solid surfaces faster than any other gliding mycoplasma. To elucidate the gliding mechanism, we developed a transformation system for M. mobile based on a transposon derived from Tn4001. Modification of the electroporation conditions, outgrowth time,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01418-13
更新日期:2014-05-01 00:00:00
abstract::Dworkin, Martin (Indiana University Medical Center, Indianapolis, Ind.). Nutritional requirements for vegetative growth of Myxococcus xanthus. J. Bacteriol. 84:250-257. 1962.-This investigation was part of a program to clarify the environmental regulation of fruiting-body formation in a fruiting myxobacterium. By use ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.2.250-257.1962
更新日期:1962-08-01 00:00:00
abstract::Proteus mirabilis swarming behavior is characterized by the development of concentric rings of growth that are formed as cyclic events of swarmer cell differentiation, swarming migration, and cellular differentiation are repeated during colony translocation across a surface. This cycle produces the bull's-eye colony o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::Uranyl nitrate inhibited the transport of several amino acids, a vitamin, and a disaccharide into yeast. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.1205-1206.1971
更新日期:1971-03-01 00:00:00
abstract::It was shown previously that the disruption of the ahrC gene encoding a predicted ArgR family transcription factor results in a severe defect in biofilm formation in vitro, as well as a significant attenuation of virulence of Enterococcus faecalis strain OG1RF in multiple experimental infection models. Using transcrip...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00269-18
更新日期:2018-08-24 00:00:00
abstract::Widely studied Salmonella enterica serovar Typhimurium strains ATCC 14028s and SL1344 harbor a cryptic ST64B prophage unable to produce infectious virions. We found that coculturing either strain with an isogenic sibling lacking the prophage leads to the appearance of active forms of the virus. Active phage originates...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.12.4038-4041.2004
更新日期:2004-06-01 00:00:00
abstract::Bacterial flagellar filaments grow at their distal ends, from flagellin that travels through a central channel ∼2 nm in diameter. The flagellin is extruded from the cytoplasm by a pump powered by a proton motive force (PMF). We measured filament growth in cells near the mid-exponential-phase with flagellin bearing a s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06735-11
更新日期:2012-05-01 00:00:00
abstract::Addition of purines to the growth medium of Escherichia coli represses synthesis of cytosine deaminase (codA) and enzymes of purine de novo synthesis. After Tn10 mutagenesis, mutants displaying derepressed levels of cytosine deaminase in the presence of hypoxanthine were isolated. One of these had simultaneously acqui...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.4.2124-2127.1989
更新日期:1989-04-01 00:00:00
abstract::The toxR gene of Vibrio cholerae encodes a transcriptional activator required for the expression of the cholera toxin genes (ctxAB) and more than 15 other genes encoding secreted or membrane proteins. The latter group includes virulence genes involved in the biogenesis of the TCP pilus, the accessory colonization fact...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.9.2842-2851.1991
更新日期:1991-05-01 00:00:00