Percent charging of transfer ribonucleic acid and levels of ppGpp and pppGpp in dormant and germinated spores of Bacillus megaterium.

Abstract:

:The levels of transfer ribonucleic acids (tRNAs) specific for 14 amino acids were almost identical in dormant spores and in spores germinated from 6 to 75 min. Germinated spore tRNAs specific for all amino acids tested were between 63 and 93% charged, and there was no significant change in this value from 6 to 75 min of germination. In contrast, tRNAs isolated from dormant spores specific for nine different amino acids were almost completely(>93%) uncharged. However, some dormant spore tRNAs, i.e., those for arginine, histidine, isoleucine, and valine, showed significant (21 to 72%) levels of aminoacylation. Dormant spores contained no detectable guanosine penta- (pppGpp), tetra- (ppGpp), or triphosphate (GTP). However, these nucleotides appeared in the first minutes of germination, and thereafter all increased in parallel with a ratio of pppGpp plus ppGpp to GTP of 0.07 to 0.11, which is characteristic of unstarved vegetative cells.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Setlow P

doi

10.1128/JB.118.3.1067-1074.1974

subject

Has Abstract

pub_date

1974-06-01 00:00:00

pages

1067-74

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

118

pub_type

杂志文章
  • Methylation of the cellular lipid of methionine-requiring Agrobacterium tumefaciens.

    abstract::Mutants of Agrobacterium tumefaciens requiring methionine for growth on a solid basal medium were induced by the use of N-methyl-N'-nitro-N-nitrosoguanidine. In addition to the difference of mutant strains, the extent of methionine dependency differed in a liquid basal medium and in the presence of aspartate or fumara...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.6.2078-2082.1968

    authors: Kaneshiro T

    更新日期:1968-06-01 00:00:00

  • COLORADO TICK FEVER VIRUS IN CELL CULTURE. I. CELL-TYPE SUSCEPTIBILITY AND INTERACTION WITH L CELLS.

    abstract::Trent, Dennis W. (University of Oklahoma School of Medicine, Oklahoma City) and L. Vernon Scott. Colorado tick fever virus in cell culture. I. Cell-type susceptibility and interaction with L cells. J. Bacteriol. 88:702-708. 1964.-Colorado tick fever (CTF) virus was serially propagated in monolayer cultures of L and FL...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.3.702-708.1964

    authors: TRENT DW,SCOTT LV

    更新日期:1964-09-01 00:00:00

  • Trypsin mediates growth phase-dependent transcriptional tegulation of genes involved in biosynthesis of ruminococcin A, a lantibiotic produced by a Ruminococcus gnavus strain from a human intestinal microbiota.

    abstract::Ruminococcin A (RumA) is a trypsin-dependent lantibiotic produced by Ruminococcus gnavus E1, a gram-positive strict anaerobic strain isolated from a human intestinal microbiota. A 12.8-kb region from R. gnavus E1 chromosome, containing the biosynthetic gene cluster of RumA, has been cloned and sequenced. It consisted ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.1.18-28.2002

    authors: Gomez A,Ladiré M,Marcille F,Fons M

    更新日期:2002-01-01 00:00:00

  • High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis.

    abstract::The master regulator for entry into sporulation in Bacillus subtilis is the response regulator Spo0A, which directly governs the expression of about 121 genes. Using cells in which the synthesis of Spo0A was under the control of an inducible promoter or in which production of the regulatory protein was impaired by a p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.4.1357-1368.2005

    authors: Fujita M,González-Pastor JE,Losick R

    更新日期:2005-02-01 00:00:00

  • Formation of molecular complexes between a structurally defined M protein and acylated or deacylated lipoteichoic acid of Streptococcus pyogenes.

    abstract::The orientation of lipoteichoic acid (LTA) molecules on the surface of bacterial cells undoubtedly is determined by the ability of the LTA, during its transit through the cell wall, to bind via its polyglycerophosphate backbone or its glycolipid moieties to other constituents of the cytoplasmic membrane and the cell w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.2.426-433.1982

    authors: Ofek I,Simpson WA,Beachey EH

    更新日期:1982-02-01 00:00:00

  • Population genetic analysis of Helicobacter pylori by multilocus enzyme electrophoresis: extensive allelic diversity and recombinational population structure.

    abstract::Genetic diversity and relationships in 74 Helicobacter pylori isolates recovered from patients assigned to distinct clinical categories were estimated by examination of allelic variation in six genes encoding metabolic housekeeping enzymes by multilocus enzyme electrophoresis. Seventy-three distinct allele profiles, r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.13.3934-3938.1996

    authors: Go MF,Kapur V,Graham DY,Musser JM

    更新日期:1996-07-01 00:00:00

  • Synergistic effect of himA and gyrB mutations: evidence that him functions control expression of ilv and xyl genes.

    abstract::We have constructed Escherichia coli strains containing mutations at two different loci, both originally selected for failure to support lambda site-specific recombination: himA and gyrB-him(Ts). Although the gyrB-him(Ts) mutations by themselves reduce supercoiling at high temperature, the double mutants show a far gr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.2.484-489.1984

    authors: Friedman DI,Olson EJ,Carver D,Gellert M

    更新日期:1984-02-01 00:00:00

  • Regulation of Escherichia coli aspartate transcarbamylase synthesis by guanosine tetraphosphate and pyrimidine ribonucleoside triphosphates.

    abstract::The effects of guanosine tetraphosphate (ppGpp) and pyrimidine ribonucleoside triphosphates on Escherichia coli aspartate transcarbamylase (ATCase) synthesis were examined. To determine the effect of ppGpp, a stringent (relA+) and relaxed (relA) isogenic pair of E. coli K-12 strains was starved for isoleucine, and the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.2.998-1007.1983

    authors: Turnbough CL Jr

    更新日期:1983-02-01 00:00:00

  • In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae.

    abstract::In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.6.2876-2880.1987

    authors: Wong PK,Popham D,Keener J,Kustu S

    更新日期:1987-06-01 00:00:00

  • A novel role for Escherichia coli endonuclease VIII in prevention of spontaneous G-->T transversions.

    abstract::In the bacterium Escherichia coli, oxidized pyrimidines are removed by two DNA glycosylases, endonuclease III and endonuclease VIII (endo VIII), encoded by the nth and nei genes, respectively. Double mutants lacking both of these activities exhibit a high spontaneous mutation frequency, and here we show that all of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.20.6396-6402.1999

    authors: Blaisdell JO,Hatahet Z,Wallace SS

    更新日期:1999-10-01 00:00:00

  • Antibacterial nitroacridine, Nitroakridin 3582: binding to nucleic acids in vitro and effects on selected cell-free model systems of macromolecular biosynthesis.

    abstract::Nitroakridin 3582 (NA) formed complexes with native deoxyribonucleic acid (DNA) and with transfer ribonucleic acid (tRNA) species from Escherichia coli. Spectrophotometric titrations of NA with these nucleic acids produced numerical results from which nonlinear adsorption isotherms were derived. These curves indicated...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.3.1026-1033.1971

    authors: Wolfe AD,Cook TM,Hahn FE

    更新日期:1971-12-01 00:00:00

  • Excision repair of thymine glycols, urea residues, and apurinic sites in Escherichia coli.

    abstract::The genetic requirements for the excision repair of thymine glycols, urea residues, and apurinic (AP) sites were examined by measuring the survival in Escherichia coli mutants of phi X174 replicative form (RF) I transfecting DNA containing selectively introduced lesions. phi X RF I DNA containing thymine glycols was i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.8.3359-3366.1988

    authors: Laspia MF,Wallace SS

    更新日期:1988-08-01 00:00:00

  • Genome sequence of the repetitive-sequence-rich Mycoplasma fermentans strain M64.

    abstract::Mycoplasma fermentans is a microorganism commonly found in the genitourinary and respiratory tracts of healthy individuals and AIDS patients. The complete genome of the repetitive-sequence-rich M. fermentans strain M64 is reported here. Comparative genomics analysis revealed dramatic differences in genome size between...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05228-11

    authors: Shu HW,Liu TT,Chan HI,Liu YM,Wu KM,Shu HY,Tsai SF,Hsiao KJ,Hu WS,Ng WV

    更新日期:2011-08-01 00:00:00

  • Anaerobic production of alginate by Pseudomonas aeruginosa: alginate restricts diffusion of oxygen.

    abstract::Pseudomonas aeruginosa produced alginate and elevated algD (encoding GDPmannose 6-dehydrogenase) transcription under strict anaerobic conditions, especially when using nitrate as a terminal electron acceptor. Purified alginate added to bacterial suspensions caused a decrease in growth, suggesting that alginate contrib...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.24.7322-7325.1996

    authors: Hassett DJ

    更新日期:1996-12-01 00:00:00

  • Oxygen-dependent regulation of the central pathway for the anaerobic catabolism of aromatic compounds in Azoarcus sp. strain CIB.

    abstract::The role of oxygen in the transcriptional regulation of the PN promoter that controls the bzd operon involved in the anaerobic catabolism of benzoate in the denitrifying Azoarcus sp. strain CIB has been investigated. In vivo experiments using PN::lacZ translational fusions, in both Azoarcus sp. strain CIB and Escheric...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.7.2343-2354.2006

    authors: Durante-Rodríguez G,Zamarro MT,García JL,Díaz E,Carmona M

    更新日期:2006-04-01 00:00:00

  • Conjugative transfer of Tn916 in Enterococcus faecalis: trans activation of homologous transposons.

    abstract::Tn916 [carries tet(M)] is a 16.4-kb conjugative transposon that can establish itself in multiple copies in Enterococcus faecalis. To study the interaction of coresident homologous transposons during conjugation, an E. faecalis mutant defective in homologous recombination was utilized for construction of strains harbor...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.22.7136-7141.1991

    authors: Flannagan SE,Clewell DB

    更新日期:1991-11-01 00:00:00

  • Biosynthesis of phospholipids in Bacillus megaterium.

    abstract::Information on the biosynthesis of phospholipids in bacteria has been derived principally from the study of Escherichia coli and other gram-negative organisms. We have now carried out a detailed study of the pathways of phospholipid biosynthesis in the gram-positive organism Bacillus megarterium KM in relation to inve...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.996-1007.1979

    authors: Langley KE,Yaffe MP,Kennedy EP

    更新日期:1979-12-01 00:00:00

  • Biochemical and phylogenetic characterization of a novel diaminopimelate biosynthesis pathway in prokaryotes identifies a diverged form of LL-diaminopimelate aminotransferase.

    abstract::A variant of the diaminopimelate (DAP)-lysine biosynthesis pathway uses an LL-DAP aminotransferase (DapL, EC 2.6.1.83) to catalyze the direct conversion of L-2,3,4,5-tetrahydrodipicolinate to LL-DAP. Comparative genomic analysis and experimental verification of DapL candidates revealed the existence of two diverged fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01381-07

    authors: Hudson AO,Gilvarg C,Leustek T

    更新日期:2008-05-01 00:00:00

  • Genome sequence of Microbacterium yannicii, a bacterium isolated from a cystic fibrosis patient.

    abstract::Microbacterium yannicii is a Gram-positive, aerobic, yellow-pigmented, rod-shaped, nonmotile, oxidase-negative, and catalase-positive bacterium isolated on Columbia colistin-nalidixic acid (CNA) agar with 5% sheep blood from the sputum of a cystic fibrosis patient. The present study reports the draft genome of a Micro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01088-12

    authors: Sharma P,Diene SM,Gimenez G,Robert C,Rolain JM

    更新日期:2012-09-01 00:00:00

  • Distinct functions of the two specificity determinants in replication initiation of plasmids ColE2-P9 and ColE3-CA38.

    abstract::The plasmid ColE2-P9 Rep protein specifically binds to the cognate replication origin to initiate DNA replication. The replicons of the plasmids ColE2-P9 and ColE3-CA38 are closely related, although the actions of the Rep proteins on the origins are specific to the plasmids. The previous chimera analysis identified tw...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01695-06

    authors: Aoki K,Shinohara M,Itoh T

    更新日期:2007-03-01 00:00:00

  • Lactate metabolism by Veillonella parvula.

    abstract::A strain of Veillonella parvula M4, which grows readily in lactate broth without a requirement for carbon dioxide, has been isolated from the oral cavity. Anaerobic, washed cells of this organism fermented sodium lactate to the following products (moles/100 moles of lactate): propionate, 66; acetate, 40; carbon dioxid...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.3.999-1005.1971

    authors: Ng SK,Hamilton IR

    更新日期:1971-03-01 00:00:00

  • Mutational analysis of the Myxococcus xanthus Omicron4499 promoter region reveals shared and unique properties in comparison with other C-signal-dependent promoters.

    abstract::The bacterium Myxococcus xanthus undergoes multicellular development during times of nutritional stress and uses extracellular signals to coordinate cell behavior. C-signal affects gene expression late in development, including that of Omega4499, an operon identified by insertion of Tn5 lac into the M. xanthus chromos...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.12.3766-3776.2004

    authors: Yoder DR,Kroos L

    更新日期:2004-06-01 00:00:00

  • Surface viscoelasticity of individual gram-negative bacterial cells measured using atomic force microscopy.

    abstract::The cell envelope of gram-negative bacteria is responsible for many important biological functions: it plays a structural role, it accommodates the selective transfer of material across the cell wall, it undergoes changes made necessary by growth and division, and it transfers information about the environment into th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00132-08

    authors: Vadillo-Rodriguez V,Beveridge TJ,Dutcher JR

    更新日期:2008-06-01 00:00:00

  • Mutation of recF, recJ, recO, recQ, or recR improves Hfr recombination in resolvase-deficient ruv recG strains of Escherichia coli.

    abstract::The formation of recombinants in Hfr crosses was studied in Escherichia coli strains carrying combinations of genes known to affect recombination and DNA repair. Mutations in ruv and recG eliminate activities that have been shown to process Holliday junction intermediates by nuclease cleavage and/or branch migration. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.6.1570-1577.1994

    authors: Ryder L,Whitby MC,Lloyd RG

    更新日期:1994-03-01 00:00:00

  • The lrp gene product regulates expression of lysU in Escherichia coli K-12.

    abstract::In Escherichia coli K-12, expression of the lysU gene is regulated by the lrp gene product, as indicated by an increase in the level of lysyl-tRNA synthetase activity and LysU protein in an lrp mutant. Comparison of the patterns of protein expression visualized by two-dimensional gel electrophoresis indicated that Lys...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.9.2779-2784.1992

    authors: Lin R,Ernsting B,Hirshfield IN,Matthews RG,Neidhardt FC,Clark RL,Newman EB

    更新日期:1992-05-01 00:00:00

  • Alpha-l-arabinofuranosidase from Rhodotorula flava.

    abstract::An alpha-L-arabinofuranosidase (EC 3.2.1.55) from the culture fluid of Rhodotorula flava IFO 0407 grown on beet arabinan as a carbon source has been highly purified. The purified enzyme has a pH optimum of 2.0. The enzyme is unusually acid stable, retaining 82% of its activity after being maintained for 24 h at pH 1.5...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.3.1073-1077.1978

    authors: Uesaka E,Sato M,Raiju M,Kaji A

    更新日期:1978-03-01 00:00:00

  • CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase, which is involved in the synthesis of the major wall teichoic acid in Bacillus subtilis 168, is encoded by tagF (rodC).

    abstract::Assays of CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase (CGPTase) (EC 2.7.8.12) in membranes isolated from Bacillus subtilis 168 wild type and 11 strains bearing conditional lethal thermosensitive mutations in tagB, tagD, or tagF revealed that CGPTase deficiency was associated only with mutant tagF all...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.2.646-649.1992

    authors: Pooley HM,Abellan FX,Karamata D

    更新日期:1992-01-01 00:00:00

  • BIOLOGY OF BUDDING BACTERIA. 3. FINE STRUCTURE OF RHODOMICROBIUM AND HYPHOMICROBIUM SPP.

    abstract::Conti, S. F. (Dartmouth Medical School, Hanover, N.H.), and Peter Hirsch. Biology of budding bacteria. III. Fine structure of Rhodomicrobium and Hyphomicrobium spp. J. Bacteriol. 89:503-512. 1965.-The ultrastructure of 14 strains of hyphomicrobia, and of Rhodomicrobium vannielii, was investigated by means of electron ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.2.503-512.1965

    authors: CONTI SF,HIRSCH P

    更新日期:1965-02-01 00:00:00

  • AmiC functions as an N-acetylmuramyl-l-alanine amidase necessary for cell separation and can promote autolysis in Neisseria gonorrhoeae.

    abstract::Neisseria gonorrhoeae is prone to undergo autolysis under many conditions not conducive to growth. The role of autolysis during gonococcal infection is not known, but possible advantages for the bacterial population include provision of nutrients to a starving population, modulation of the host immune response by rele...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00724-06

    authors: Garcia DL,Dillard JP

    更新日期:2006-10-01 00:00:00

  • Mutations in the araC regulatory gene of Escherichia coli B/r that affect repressor and activator functions of AraC protein.

    abstract::Mutations in the araC gene of Escherichia coli B/r were isolated which alter both activation of the araBAD operon expression and autoregulation. The mutations were isolated on an araC-containing plasmid by hydroxylamine mutagenesis of plasmid DNA. The mutant phenotype selected was the inability to autoregulate. The DN...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.3.892-900.1986

    authors: Cass LG,Wilcox G

    更新日期:1986-06-01 00:00:00