Abstract:
:Addition of purines to the growth medium of Escherichia coli represses synthesis of cytosine deaminase (codA) and enzymes of purine de novo synthesis. After Tn10 mutagenesis, mutants displaying derepressed levels of cytosine deaminase in the presence of hypoxanthine were isolated. One of these had simultaneously acquired resistance to the hypoxanthine analog 6-mercaptopurine. The mutation purR6::Tn10 was shown to affect de novo synthesis of the purine enzymes glutamine phosphoribosylpyrophosphate amidotransferase (purF) and phosphoribosyl glycinamide synthetase (purD). The mutation was mapped by P1 transduction at 36 min on the E. coli linkage map. A plasmid containing the purR region was obtained by complementation of the purR6::Tn10 mutation. By comparing the restriction maps of the cloned fragment and the E. coli chromosome, the purR gene was found to be located very close to the lpp gene (36.3 min).
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kilstrup M,Meng LM,Neuhard J,Nygaard Pdoi
10.1128/jb.171.4.2124-2127.1989subject
Has Abstractpub_date
1989-04-01 00:00:00pages
2124-7issue
4eissn
0021-9193issn
1098-5530journal_volume
171pub_type
杂志文章abstract::Respiratory nitrate reductase purified from the cell membrane of Escherichia coli is composed of three subunits, alpha, beta, and gamma, which are encoded, respectively, by the narG, narH, and narI genes of the narGHJI operon. The product of the narJ gene was deduced previously to be a highly charged, acidic protein w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.3.867-872.1992
更新日期:1992-02-01 00:00:00
abstract::The AOX1 gene, which encodes an alternative oxidase, was isolated from the genomic DNA library of Candida albicans. The gene encodes a polypeptide consisting of 379 amino acids with a calculated molecular mass of 43,975 Da. The aox1/aox1 mutant strain did not show cyanide-resistant respiration under normal conditions ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.13.4098-4102.1999
更新日期:1999-07-01 00:00:00
abstract::The P1 plasmid addiction operon is a compact genetic structure consisting of promoter, operator, antitoxin gene (phd), and toxin gene (doc). The 73-amino-acid antitoxin protein, Phd, has two distinct functions: it represses transcription (by binding to its operator) and it prevents host death (by binding and neutraliz...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.9.2692-2698.2004
更新日期:2004-05-01 00:00:00
abstract::The lmaA gene of Listeria monocytogenes encodes a protein capable of inducing delayed-type hypersensitivity reactions in L. monocytogenes-immune mice (S. Göhmann, M. Leimeister-Wachter, E. Schiltz, W. Goebel, and T. Chakraborty, M. Microbiol. 4:1091-1099, 1990). Here we show that it is the last gene of the lma operon,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.8.2707-2716.1997
更新日期:1997-04-01 00:00:00
abstract::Chlamydiae are unusual obligately intracellular bacteria that do not synthesize detectable peptidoglycan. However, they possess genes that appear to encode products with peptidoglycan biosynthetic activity. Bioinformatic analysis predicts that chlamydial MurE possesses UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelic acid (...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01029-09
更新日期:2009-12-01 00:00:00
abstract::Three genes were shown to provide functions specific for ferric enterobactin transport in Escherichia coli: fepA encoded the outer membrane receptor, fepB produced a periplasmic protein, and the fepC product was presumably a component of a cytoplasmic membrane permease system for this siderophore. A 10.6-kilobase-pair...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3638-3646.1987
更新日期:1987-08-01 00:00:00
abstract::The major facilitator superfamily includes a large collection of evolutionarily related proteins that have been implicated in the transport of a variety of solutes and metabolites across the membranes of organisms ranging from bacteria to humans. We have recently reported the three-dimensional structure, at 6.5 A reso...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.5.1712-1718.2003
更新日期:2003-03-01 00:00:00
abstract::We describe the derivation of two strains of Salmonella typhimurium LT2 which are r- m+ for all three of the known chromosomal genes for the restriction and modification of DNA, hsdLT, hsdSA, and hsdSB; the strains were designated LB5000 and LB5010. LB5000 is a smooth derivative sensitive to phage P22; LB5010 is a gal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.1.471-474.1983
更新日期:1983-10-01 00:00:00
abstract::Quantitative mass conversion of intact bacterial cells of Bacillus subtilis and B. licheniformis to L-phase variants has been effected after lysozyme treatment. After subculture of the unstable L-phase variants for several months in the presence of methicillin, stable L-phase variants were obtained which grew in the a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.1.456-465.1973
更新日期:1973-10-01 00:00:00
abstract::Filter matings between E. coli K-12 strains carrying an F'::Tn5,Tn9 factor with H. influenzae Rd strains gave rise to kanamycin-chloramphenicol-resistant H. influenzae strains at a frequency of approximately 10(-6). Transfer of the F' factor to H. influenzae was verified by expression of unselected markers in H. influ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.1905-1910.1987
更新日期:1987-05-01 00:00:00
abstract::We designed a selection strategy for the isolation of Escherichia coli mutants exhibiting enhanced protein disulfide isomerase activity. The folding of a variant of tissue plasminogen activator (v-tPA), a protein containing nine disulfide bonds, in the bacterial periplasm is completely dependent on the level of disulf...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.3.654-660.2004
更新日期:2004-02-01 00:00:00
abstract::Cells of Clostridium thermosaccharolyticum grown under strict anaerobiosis (modified Hungate technique) were examined during growth and sporulation by employing Nomarski interference-contrast and Zernike phase-contrast optics to delineate the sequence of morphological changes leading to the formation of free, mature s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.3.674-681.1972
更新日期:1972-09-01 00:00:00
abstract::Bacteria possess multiple mechanisms to survive exposure to various chemical stresses and antimicrobial compounds. In the enteric bacterium Escherichia coli, three homologous transcription factors-MarA, SoxS, and Rob-play a central role in coordinating this response. Three separate systems are known to regulate the ex...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00680-12
更新日期:2012-09-01 00:00:00
abstract::The fumarate reductase enzyme complex allows Escherichia coli to grow anaerobically with fumarate as a terminal electron acceptor for oxidative phosphorylation when the preferred compounds oxygen and nitrate are not available. We used the pKO promoter test vectors to identify a single promoter for the frdABCD genes wh...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.3.1100-1109.1985
更新日期:1985-12-01 00:00:00
abstract::We have sequenced a DNA fragment containing the pneumococcal bacteriophage HB-3 hbl gene, which codes for the phage lytic amidase. A remarkable nucleotide similarity (87.1%) between the lytA gene, coding for the pneumococcal amidase, the major autolysin of Streptococcus pneumoniae, and the hbl gene was found. This sim...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.9.5064-5070.1990
更新日期:1990-09-01 00:00:00
abstract::The extracellular chlamydial infectious particle, or elementary body (EB), is enveloped by an intra- and intermolecular cysteine cross-linked protein shell called the chlamydial outer membrane complex (COMC). A few abundant proteins, including the major outer membrane protein and cysteine-rich proteins (OmcA and OmcB)...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01628-09
更新日期:2010-06-01 00:00:00
abstract::The structural gene for histidyl-tRNA synthetase was localized to 53.8 min on the Escherichia coli genome. The gene order in this region was determined to be dapE-purC-upp-purG-(guaA, guaB)-hisS-glyA. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.1.264-267.1979
更新日期:1979-04-01 00:00:00
abstract::The intervals between rounds of chromosome and minichromosome replication were measured by density shift experiments and found to be similar. Thus the minichromosome, a lambda asnA oriC bacteriophage, mostly replicates once each division cycle rather than randomly, despite its high copy number. Slight differences betw...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.1.430-433.1987
更新日期:1987-01-01 00:00:00
abstract::Transcription of the gyrA and gyrB genes, which encode the subunits of DNA gyrase, increases in response to DNA relaxation. Previous studies have shown that a small segment of DNA extending from the -10 consensus hexamer to the start of transcription encodes the sequence determinants for this response. In this study, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.19.5999-6006.1994
更新日期:1994-10-01 00:00:00
abstract:UNLABELLED:Campylobacter jejuni is a leading cause of bacterial diarrheal disease and a frequent commensal of the intestinal tract in poultry and other animals. For optimal growth and colonization of hosts, C. jejuni employs two-component regulatory systems (TCSs) to monitor environmental conditions and promote proper ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02564-14
更新日期:2015-05-01 00:00:00
abstract::Twelve strains of Agrobacterium radiobacter isolated from naturally occurring crown galls or soil were found to be avirulent on sunflower, tomato, Kalanchoe, and carrot. Eleven strains contained plasmids of molecular weights 77 X 10(6) to 182 X 10(6) as determined by electron microscopy. One strain contained only a sm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.1.76-80.1977
更新日期:1977-01-01 00:00:00
abstract::Toxin-antitoxin (TA) loci consist of two genes in an operon, encoding a stable toxin and an unstable antitoxin. The expression of toxin leads to cell growth arrest and sometimes bacterial death, while the antitoxin prevents the cytotoxic activity of the toxin. In this study, we show that the chromosome of Yersinia pes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00336-10
更新日期:2010-07-01 00:00:00
abstract::The staphylococcal virulon is activated by the density-sensing agr system, which is autoinduced by a short peptide (autoinducing peptide [AIP]) processed from a propeptide encoded by agrD. A central segment of the agr locus, consisting of the C-terminal two-thirds of AgrB (the putative processing enzyme), AgrD, and th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.22.6517-6522.2000
更新日期:2000-11-01 00:00:00
abstract::A macrorestriction map representing the complete physical map of the Rhodobacter sphaeroides 2.4.1 chromosomes has been constructed by ordering the chromosomal DNA fragments from total genomic DNA digested with the restriction endonucleases AseI, SpeI, DraI, and SnaBI. Junction fragments and multiple restriction endon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.5850-5859.1989
更新日期:1989-11-01 00:00:00
abstract::Korormicin is an antibiotic produced by some pseudoalteromonads which selectively kills Gram-negative bacteria that express the Na+-pumping NADH:quinone oxidoreductase (Na+-NQR.) We show that although korormicin is an inhibitor of Na+-NQR, the antibiotic action is not a direct result of inhibiting enzyme activity. Ins...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00718-18
更新日期:2019-05-08 00:00:00
abstract::Sequencing 16S rRNA genes (SSU) cloned from Aeromonas strains revealed that strains contained up to six copies differing by < or = 1.5%. The SSU copies from Aeromonas veronii LMG13695 clustered with sequences from four Aeromonas species. These results demonstrate intragenomic heterogeneity of SSU and suggest caution w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.18.6561-6564.2005
更新日期:2005-09-01 00:00:00
abstract::Payne, W. J. (University of Georgia, Athens), and R. L. Todd. Flavin-linked dehydrogenation of ether glycols by cell-free extracts of a soil bacterium. J. Bacteriol. 91:1533-1536. 1966.-Cell-free extracts of bacterium TEG-5 grown on tetraethylene glycol dehydrogenated a variety of ether glycols and nonylphenoxy and se...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.4.1533-1536.1966
更新日期:1966-04-01 00:00:00
abstract::In bacteria like Escherichia coli, the accumulation of glucose-6-phosphate (G6P) or its analogs such as α-methyl glucoside-6-phosphate (αMG6P) results in stress that appears in the form of growth inhibition. The small RNA SgrS is an essential part of the response that helps E. coli combat glucose-phosphate stress; the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00705-13
更新日期:2013-11-01 00:00:00
abstract::The nucleotide sequence of the DNA encoding the ADP-ribosyltransferase (A1) fragment of cholera enterotoxin was determined. A putative precursor of the A1 peptide contains an 18-amino acid leader peptide, and the mature A1 peptide contains 194 amino acids. The primary structure of the A1 fragment from cholera enteroto...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.3.1086-1089.1984
更新日期:1984-09-01 00:00:00
abstract::Weiss, Emilio (Naval Medical Research Institute, Bethesda, Md.) and Harry R. Dressler. Increased resistance to chloramphenicol in Rickettsia prowazekii with a note on failure to demonstrate genetic interaction among strains. J. Bacteriol. 83:409-414. 1962.-A further effort to produce genetically labeled Rickettsia pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.2.409-414.1962
更新日期:1962-02-01 00:00:00