Identification of Chlamydia trachomatis outer membrane complex proteins by differential proteomics.

Abstract:

:The extracellular chlamydial infectious particle, or elementary body (EB), is enveloped by an intra- and intermolecular cysteine cross-linked protein shell called the chlamydial outer membrane complex (COMC). A few abundant proteins, including the major outer membrane protein and cysteine-rich proteins (OmcA and OmcB), constitute the overwhelming majority of COMC proteins. The identification of less-abundant COMC proteins has been complicated by limitations of proteomic methodologies and the contamination of COMC fractions with abundant EB proteins. Here, we used parallel liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) analyses of Chlamydia trachomatis serovar L2 434/Bu EB, COMC, and Sarkosyl-soluble EB fractions to identify proteins enriched or depleted from COMC. All well-described COMC proteins were specifically enriched in the COMC fraction. In contrast, multiple COMC-associated proteins found in previous studies were strongly enriched in the Sarkosyl-soluble fraction, suggesting that these proteins are not COMC components or are not stably associated with COMC. Importantly, we also identified novel proteins enriched in COMC. The list of COMC proteins identified in this study has provided reliable information for further understanding chlamydial protein secretion systems and modeling COMC and EB structures.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Liu X,Afrane M,Clemmer DE,Zhong G,Nelson DE

doi

10.1128/JB.01628-09

subject

Has Abstract

pub_date

2010-06-01 00:00:00

pages

2852-60

issue

11

eissn

0021-9193

issn

1098-5530

pii

JB.01628-09

journal_volume

192

pub_type

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