Abstract:
:Transposition is a DNA reorganization reaction potentially deleterious for the host. The frequency of transposition is limited by the amount of transposase. Therefore, strict regulation of a transposase is required to keep control over the destructive multiplication of the mobile element. We have shown previously that the expression of the transposase (tnpA) of the Pseudomonas putida PaW85 transposon Tn4652 is positively affected by integration host factor. Here, we present evidence that the amount of the transposase of Tn4652 in P. putida cells is controlled by the transposon-encoded protein (TnpC). Sequence analysis of the 120-amino-acid-long TnpC, coded just downstream of the tnpA gene, showed that it has remarkable similarity to the putative polypeptide encoded by the mercury resistance transposon Tn5041. As determined by quantitative Western blot analysis, the abundance of TnpA was reduced up to 10-fold in the intact tnpC background. In vivo experiments using transcriptional and translational fusions of the tnpA gene and the reporter gene gusA indicated that TnpC operates in the regulation of the transposase of Tn4652 at the post-transcriptional level.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hõrak R,Kivisaar Mdoi
10.1128/JB.181.20.6312-6318.1999keywords:
subject
Has Abstractpub_date
1999-10-01 00:00:00pages
6312-8issue
20eissn
0021-9193issn
1098-5530journal_volume
181pub_type
杂志文章abstract::Campylobacter jejuni is a leading food-borne pathogen causing gastroenteritis in humans. Although OxyR is a widespread oxidative stress regulator in many Gram-negative bacteria, C. jejuni lacks OxyR and instead possesses the metalloregulator PerR. Despite the important role played by PerR in oxidative stress defense, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05493-11
更新日期:2011-11-01 00:00:00
abstract::The DnaA protein concentration in Escherichia coli was increased above the wild-type level by inducing a lacP-controlled dnaA gene located on a plasmid. In these cells with different DnaA protein levels, we measured several parameters: dnaA gene expression; cell size, amount of DNA per cell, and number of origins per ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.20.6537-6545.1993
更新日期:1993-10-01 00:00:00
abstract::The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.15.4544-4548.1995
更新日期:1995-08-01 00:00:00
abstract::The reproduction and morphogenesis of Rickettsiella chironomi, an unusual procaryotic parasite of midge larvae, was studied with electron microscopy. The morphogenic cycle was similar to that found in chlamydia and consisted of four major cell types: (i) medium-sized spherical initial bodies (ca. 1 micrometers in diam...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.2.995-1002.1980
更新日期:1980-08-01 00:00:00
abstract::Transcriptional fusions to Salmonella typhimurium chromosomal genes were constructed by integration of a suicide fusion vector into the chromosome by homologous recombination with random cloned chromosomal fragments. We describe here a transductional method using the generalized transducing phage of S. typhimurium, P2...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.21.7086-7091.1993
更新日期:1993-11-01 00:00:00
abstract::The RecA proteins of the unusually strong protease-constitutive mutants recA1202 and recA1211 can use RNA in addition to single-stranded DNA (ssDNA) as a cofactor in the cleavage of the LexA repressor in vitro. In the presence of rRNA or tRNA, the effectiveness of these proteins decreased in the order RecA1202 greater...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4823-4827.1988
更新日期:1988-10-01 00:00:00
abstract::Trent, Dennis W. (University of Oklahoma School of Medicine, Oklahoma City) and L. Vernon Scott. Colorado tick fever virus in cell culture. I. Cell-type susceptibility and interaction with L cells. J. Bacteriol. 88:702-708. 1964.-Colorado tick fever (CTF) virus was serially propagated in monolayer cultures of L and FL...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.3.702-708.1964
更新日期:1964-09-01 00:00:00
abstract::Pseudomonas aeruginosa PAO1 catabolized the aromatic amines tyramine and octopamine through 4-hydroxyphenylacetic acid and 3,4-dihydroxyphenylacetic acid (HPA). meta ring cleavage was mediated by 3,4-dihydroxyphenylacetate 2,3-dioxygenase (HPADO), producing 2-hydroxy-5-carboxymethylmuconic semialdehyde (MSA). An NAD-d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.393-399.1988
更新日期:1988-01-01 00:00:00
abstract::The 6th American Society for Microbiology Conference on Cell-Cell Communication in Bacteria convened from 16 to 19 October 2017 in Athens, GA. In this minireview, we highlight some of the research presented at that meeting that addresses central questions emerging in the field, including the following questions. How a...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00291-18
更新日期:2018-06-25 00:00:00
abstract::In Staphylococcus aureus, the transport of dicarboxylates is mediated in part by the Na+-linked carrier protein SdcS. This transporter is a member of the divalent-anion/Na+ symporter (DASS) family, a group that includes the mammalian Na+/dicarboxylate cotransporters NaDC1 and NaDC3. In earlier work, we cloned and expr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01452-06
更新日期:2007-02-01 00:00:00
abstract::The reactive alpha-oxoaldehydes such as glyoxal (GO) and methylglyoxal (MG) are generated in vivo from sugars through oxidative stress. GO and MG are believed to be removed from cells by glutathione-dependent glyoxalases and other aldehyde reductases. We isolated a number of GO-resistant (GO(r)) mutants from Escherich...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01127-09
更新日期:2010-08-01 00:00:00
abstract::Escherichia coli acetyl coenzyme A carboxylase (ACC), the first enzyme of the fatty acid biosynthetic pathway, is inhibited by acylated derivatives of acyl carrier protein (ACP). ACP lacking an acyl moiety does not inhibit ACC. Acylated derivatives of ACP having chain lengths of 6 to 20 carbon atoms were similarly inh...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.4.1499-1503.2001
更新日期:2001-02-01 00:00:00
abstract::An attempt was made to explain the puzzling observation that in bacteria 2,6-diaminopurine can replace guanine for guanineless mutants and for xanthineless mutants (both of which can make adenosine monophosphate de novo) but not for nonexacting purine auxotrophs (which cannot make adenosine monophosphate de novo). The...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.2.565-571.1968
更新日期:1968-02-01 00:00:00
abstract::We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis. Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01092-06
更新日期:2007-01-01 00:00:00
abstract::We screened Salmonella typhimurium, Citrobacter freundii, Klebsiella pneumoniae, Shigella boydii, and many isolates of Escherichia coli for DNA sequences homologous to those encoding each of two unrelated type I restriction and modification systems (EcoK and EcoA). Both K- and A-related hsd genes were identified, but ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1775-1782.1988
更新日期:1988-04-01 00:00:00
abstract::The lipid A components of substrate cell origin incorporated by Bdellovibrio bacteriovorus during intraperiplasmic growth (D. R. Nelson and S. C. Rittenberg, J. Bacteriol. 147:860-868, 1981) were shown to be integrated into its lipopolysaccharide structure. Lipid A isolated from bdellovibrios grown on Escherichia coli...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.3.869-874.1981
更新日期:1981-09-01 00:00:00
abstract::The gene encoding OprF, a major outer membrane protein in Pseudomonas species (formerly known as type 1 pseudomonads), was thought to be constitutively transcribed from a single sigma 70 promoter immediately upstream of the gene. We now report the identification of a novel putative ECF (extracytoplasmic function) sigm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.16.4746-4754.1999
更新日期:1999-08-01 00:00:00
abstract::Gene III (gIII) of phiLf, a filamentous phage specifically infecting Xanthomonas campestris pv. campestris, was previously shown to encode a virion-associated protein (pIII) required for phage adsorption. In this study, the transcription start site for the gene and the N-terminal sequence of the protein were determine...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.8.2465-2471.1999
更新日期:1999-04-01 00:00:00
abstract::ATP sulfurylase is a key enzyme in the energy-generating sulfur oxidation pathways of many chemoautotrophic bacteria. The utilization of reduced sulfur compounds to fuel CO2 fixation by the still-uncultured bacterial endosymbionts provides the basis of nutrition in invertebrates, such as the tubeworm Riftia pachyptila...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.12.3723-3729.1994
更新日期:1994-06-01 00:00:00
abstract::The filamentous cyanobacterium Anabaena sp. strain PCC 7120 forms a periodic pattern of nitrogen-fixing heterocysts when grown in the absence of combined nitrogen. PatA is necessary for proper patterning of heterocysts along filaments. In this study, apparent transcriptional start points (tsps) were identified at nucl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00577-10
更新日期:2010-09-01 00:00:00
abstract::Natural and immune human antibodies reactive with heat-labile and heat-stable antigens of virulent Neisseria gonorrhoeae were studied by use of an indirect fluorescent-antibody (IFA) procedure. The immunoglobulin class of the reactive antibodies was identified by using fluorescein-conjugated antisera specific for huma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.1.141-148.1967
更新日期:1967-07-01 00:00:00
abstract::In Bacillus subtilis the citM gene encodes the Mg(2+)-citrate transporter. A target site for carbon catabolite repression (cre site) is located upstream of citM. Fusions of the citM promoter region, including the cre sequence, to the beta-galactosidase reporter gene were constructed and integrated into the amyE site o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.21.6099-6105.2000
更新日期:2000-11-01 00:00:00
abstract::The high-potential 4Fe-4S* center ofAzotobacter vinelandii ferredoxin I has been titrated potentiometrically by a reductive procedure. The absorbance decrease at 510 nm accompanying the reduction of the high-potential center titrated with an Em of 320 mV (n = 1). The low-potential 4Fe-4S* center was titrated by using ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.2.822-824.1978
更新日期:1978-11-01 00:00:00
abstract::Entomopathogenic bacteria of the genus Photorhabdus harbor a type III secretion system. This system was probably acquired prior to the separation of the species within this genus. Furthermore, the core components of the secretion machinery are highly conserved but the predicted effectors differ between Photorhabdus lu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.13.4376-4381.2004
更新日期:2004-07-01 00:00:00
abstract::The surface structure and cell envelope layers of various virulent Bacteroides nodosus strains were examined by light microscopy and by electron microscopy by using negative staining, thin-section, and freeze-fracture-etch techniques. Three surface structures were described: pili and a diffuse material, both of which ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.2.845-857.1980
更新日期:1980-02-01 00:00:00
abstract::Incubation of whole envelopes prepared from sonically oscillated Escherichia coli K-12 cultures with lysozyme in vitro resulted in the appearance of a protein species with an apparent molecular weight double that of outer membrane protein I. Similar dimers were also detected in purified outer membranes and whole envel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.2.411-418.1977
更新日期:1977-11-01 00:00:00
abstract::Integrons are genetic elements that contribute to lateral gene transfer in bacteria as a consequence of possessing a site-specific recombination system. This system facilitates the spread of genes when they are part of mobile cassettes. Most integrons are contained within chromosomes and are confined to specific bacte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01950-05
更新日期:2006-08-01 00:00:00
abstract::The genome of Halobacterium sp. strain NRC-1 contains a large gene cluster, gvpMLKJIHGFEDACNO, that is both necessary and sufficient for the production of buoyant gas-filled vesicles. Due to the resistance of gas vesicles to solubilization, only the major gas vesicle protein GvpA and a single minor protein, GvpC, were...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.10.3182-3186.2004
更新日期:2004-05-01 00:00:00
abstract::An incompetent mutant (4S Com(-)) does not release competence-inducing factor (CF) into the culture medium and is not infected with TP-1C phage deoxyribonucleic acid (DNA) unless CF is added to the transfection assay. A TP-1C phage-resistant mutant (4S Ton-r) releases relatively large amounts of CF into the culture me...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.3.955-959.1971
更新日期:1971-06-01 00:00:00
abstract::Doxtader, K. G. (Cornell University, Ithaca, N.Y.), and M. Alexander. Role of 3-nitropropanoic acid in nitrate formation by Aspergillus flavus. J. Bacteriol. 91:1186-1191. 1966.-Aspergillus flavus formed nitrate, 3-nitropropanoic acid (3-NPA), kojic acid, and a substance tentatively identified as N-formyl-N-hydroxy-gl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.3.1186-1191.1966
更新日期:1966-03-01 00:00:00