The adsorption protein genes of Xanthomonas campestris filamentous phages determining host specificity.

Abstract:

:Gene III (gIII) of phiLf, a filamentous phage specifically infecting Xanthomonas campestris pv. campestris, was previously shown to encode a virion-associated protein (pIII) required for phage adsorption. In this study, the transcription start site for the gene and the N-terminal sequence of the protein were determined, resulting in the revision of the translation initiation site from the one previously predicted for this gene. For comparative study, the gIII of phiXv, a filamentous phage specifically infecting X. campestris pv. vesicatoria, was cloned and sequenced. The deduced amino acid sequences of these two pIIIs exhibit a high degree of identity in their C-terminal halves and possess the structural features typical of the adsorption proteins of filamentous phages: a signal sequence in the N terminus, a long glycine-rich region near the center, and a hydrophobic membrane anchorage domain in the C terminus. The regions between gIII and the upstream gVIII, 128 nucleotides in both phages, are larger than those of other filamentous phages. A hybrid phage of phiXv, consisting of the phiLf pIII and all the other components derived from phiXv, was able to infect X. campestris pv. campestris but not X. campestris pv. vesicatoria, indicating that gIII is the gene specifying host specificity and demonstrating the interchangeability of the pIIIs.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Lin NT,Liu TJ,Lee TC,You BY,Yang MH,Wen FS,Tseng YH

doi

10.1128/JB.181.8.2465-2471.1999

keywords:

subject

Has Abstract

pub_date

1999-04-01 00:00:00

pages

2465-71

issue

8

eissn

0021-9193

issn

1098-5530

journal_volume

181

pub_type

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