Abstract:
:The S-layers of Thermus thermophilus HB27 and T. thermophilus HB8 are composed of protein units of 95 kDa (P95) and 100 kDa (P100), respectively. We have selected S-layer deletion mutants from both strains by complete replacement of the slpA gene. Mutants of the two strains showed similar defects in growth and morphology and overproduced an external cell envelope inside of which cells remained after division. However, the nature of this external layer is strain specific, being easily stained and regular in the HB8 delta slpA derivative and amorphous and poorly stained in the HB27 delta slpA strain. The addition of chromosomic DNA from T. thermophilus HB8 to growing cultures of T. thermophilus HB27 delta slpA led to the selection of a new strain, HB27C8, which expressed a functional S-layer composed of the P100 protein. Conversely, the addition of chromosomic DNA from T. thermophilus HB27 to growing cultures of T. thermophilus HB8 delta slpA allowed the isolation of strain HB8C27, which expressed a functional S-layer composed of the P95 protein. The driving force which selected the transference of the S-layer genes in these experiments was the difference in growth rates, one of the main factors leading to selection in natural environments.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Fernández-Herrero LA,Olabarría G,Castón JR,Lasa I,Berenguer Jdoi
10.1128/jb.177.19.5460-5466.1995subject
Has Abstractpub_date
1995-10-01 00:00:00pages
5460-6issue
19eissn
0021-9193issn
1098-5530journal_volume
177pub_type
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更新日期:1969-04-01 00:00:00
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更新日期:1995-11-01 00:00:00
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更新日期:2012-02-01 00:00:00