High-frequency variation in Mycoplasma pulmonis colony size.

abstract：:Branching enzyme (BE) catalyzes formation of the branch points in glycogen and amylopectin by cleavage of the alpha-1,4 linkage and its subsequent transfer to the alpha-1,6 position. We have identified a novel BE encoded by an uncharacterized open reading frame (TK1436) of the hyperthermophilic archaeon Thermococcus k...

journal_title：Journal of bacteriology

authors： Murakami T,Kanai T,Takata H,Kuriki T,Imanaka T

更新日期：2006-08-01 00:00:00

abstract：:From Xanthomonas campestris pv. phaseoli, we have isolated by two independent methods genes involved in peroxide detoxification (ahpC and ahpF), a gene involved in peroxide sensing and transcription regulation (oxyR), and a gene of unknown function (orfX). Amino acid sequence analysis of AhpC, AhpF, and OxyR showed hi...

journal_title：Journal of bacteriology

authors： Loprasert S,Atichartpongkun S,Whangsuk W,Mongkolsuk S

更新日期：1997-06-01 00:00:00

abstract：:A series of studies were performed on a Staphylococcus aureus strain thought to contain a pencillinase plasmid integrated into the host chromosome. Reassociation kinetics analysis of whole-cell deoxyribonucleic acid (DNA) in the presence of pure radioactive plasmid DNA revealed that plasmid-specific sequences were pre...

journal_title：Journal of bacteriology

authors： Novick R,Zouzias D,Rush M

更新日期：1975-12-01 00:00:00

abstract：:Pseudomonas aeruginosa is usually resistant to a wide variety of antibacterial agents, and it has been inferred, on the basis of indirect evidence, that this was due to the low permeability of its outer membrane. We determined the permeability of P. aeruginosa outer membrane directly, by measuring the rates of hydroly...

journal_title：Journal of bacteriology

authors： Yoshimura F,Nikaido H

更新日期：1982-11-01 00:00:00

abstract：:Entomopathogenic bacteria of the genus Photorhabdus harbor a type III secretion system. This system was probably acquired prior to the separation of the species within this genus. Furthermore, the core components of the secretion machinery are highly conserved but the predicted effectors differ between Photorhabdus lu...

journal_title：Journal of bacteriology

authors： Brugirard-Ricaud K,Givaudan A,Parkhill J,Boemare N,Kunst F,Zumbihl R,Duchaud E

更新日期：2004-07-01 00:00:00

abstract：:After inoculation of a stationary-phase culture of Nitrosomonas europaea into fresh growth solution, the cell-associated orthophosphate increased rapidly to 800 mumoles/g (wet weight), whereas the acid-insoluble long-chain polyphosphate content decreased rapidly to 22 mumoles/g. As growth proceeded, the orthophosphate...

journal_title：Journal of bacteriology

authors： Terry KR,Hooper AB

更新日期：1970-07-01 00:00:00

abstract：:The reactive alpha-oxoaldehydes such as glyoxal (GO) and methylglyoxal (MG) are generated in vivo from sugars through oxidative stress. GO and MG are believed to be removed from cells by glutathione-dependent glyoxalases and other aldehyde reductases. We isolated a number of GO-resistant (GO(r)) mutants from Escherich...

journal_title：Journal of bacteriology

authors： Lee C,Kim I,Lee J,Lee KL,Min B,Park C

更新日期：2010-08-01 00:00:00

abstract：:A new insertion sequence, IS1634, has been identified in Mycoplasma mycoides subsp. mycoides small-colony type (SC). IS1634 shows structural and functional similarities to IS1549 of Mycobacterium smegmatis and with it seems to form a new class or family of insertion sequences. IS1634 has a size of 1,872 bp, including ...

journal_title：Journal of bacteriology

authors： Vilei EM,Nicolet J,Frey J

更新日期：1999-02-01 00:00:00

abstract：:Vibrio cholerae is a natural inhabitant of aquatic ecosystems. Some strains of V. cholerae can colonize the human host and cause cholera, a profuse watery diarrhea. The major pathogenicity factors and virulence regulators of V. cholerae are either encoded in mobile genetic elements acquired in the environment (e.g. pa...

journal_title：Journal of bacteriology

authors： Sakib SN,Reddi G,Almagro-Moreno S

更新日期：2018-03-26 00:00:00

abstract：:The genes for biosynthesis of the branched-chain amino acids leucine, isoleucine, and valine in Lactococcus lactis subsp. lactis NCDO2118 were characterized by cloning, complementation in Escherichia coli and Bacillus subtilis, and nucleotide sequence analysis. Nine structural genes are clustered on a 12-kb DNA fragme...

journal_title：Journal of bacteriology

authors： Godon JJ,Chopin MC,Ehrlich SD

更新日期：1992-10-01 00:00:00

abstract：:The yeast two-hybrid assay revealed that Bacillus subtilis DnaD, a possible component of the primosome and required for replication initiation, interacted with DnaA and DnaD itself. The mutant DnaD23 was incapable of interacting with DnaA but retained interaction with the wild-type DnaD. These results suggest that int...

journal_title：Journal of bacteriology

authors： Ishigo-Oka D,Ogasawara N,Moriya S

更新日期：2001-03-01 00:00:00

abstract：:Fundamental questions in bacterial gene regulation concern how multiple regulatory proteins interact with the transcription apparatus at a single promoter and what are the roles of protein contacts with RNA polymerase and changes in DNA conformation. Transcription of the Escherichia coli uhpT gene, encoding the induci...

journal_title：Journal of bacteriology

authors： Olekhnovich IN,Kadner RJ

更新日期：1999-12-01 00:00:00

abstract：:We have sequenced a DNA fragment containing the pneumococcal bacteriophage HB-3 hbl gene, which codes for the phage lytic amidase. A remarkable nucleotide similarity (87.1%) between the lytA gene, coding for the pneumococcal amidase, the major autolysin of Streptococcus pneumoniae, and the hbl gene was found. This sim...

journal_title：Journal of bacteriology

authors： Romero A,Lopez R,Garcia P

更新日期：1990-09-01 00:00:00

abstract：:Production of deoxyribonucleotides for DNA synthesis is an essential and tightly regulated process. The class Ia ribonucleotide reductase (RNR), the product of the nrdAB genes, is required for aerobic growth of Escherichia coli. In catalyzing the reduction of ribonucleotides, two of the cysteines of RNR become oxidize...

journal_title：Journal of bacteriology

authors： Feeney MA,Ke N,Beckwith J

更新日期：2012-03-01 00:00:00

abstract：:The opportunistic bacterial pathogen Vibrio vulnificus causes severe wound infection and fatal septicemia. We used alkaline phosphatase insertion mutagenesis in a clinical isolate of V. vulnificus to find genes necessary for virulence, and we identified fadR, which encodes a regulator of fatty acid metabolism. The fad...

journal_title：Journal of bacteriology

authors： Brown RN,Gulig PA

更新日期：2008-12-01 00:00:00

abstract：:Site-directed mutagenesis has been used to identify a number of charged residues essential for the transport activity of the PheP protein. These residues are highly conserved in the cluster of amino acid transporters. However, some other conserved residues and a number of aromatic residues have been shown not to be es...

journal_title：Journal of bacteriology

authors： Pi J,Wookey PJ,Pittard AJ

更新日期：1993-11-01 00:00:00

abstract：:A Flavobacterium sp. (strain 50001), capable of degrading 2,4-dichlorophenoxyacetate (2,4-D), 2-methyl-4-chlorophenoxyacetate, and 2-chlorobenzoate and imparting resistance to mercury, harbored a degradative plasmid, pRC10. Cured strains of the Flavobacterium sp. lost the plasmid as well as the ability to degrade thes...

journal_title：Journal of bacteriology

authors： Chaudhry GR,Huang GH

更新日期：1988-09-01 00:00:00

abstract：:The virulence of Pseudomonas aeruginosa and other surface pathogens involves the coordinate expression of a wide range of virulence determinants, including type IV pili. These surface filaments are important for the colonization of host epithelial tissues and mediate bacterial attachment to, and translocation across, ...

journal_title：Journal of bacteriology

authors： Leech AJ,Mattick JS

更新日期：2006-12-01 00:00:00

abstract：:Virgibacillus halodenitrificans 1806 is an endospore-forming halophilic bacterium isolated from salterns in Korea. Here, we report the draft genome sequence of V. halodenitrificans 1806, which may reveal the molecular basis of osmoadaptation and insights into carbon and anaerobic metabolism in moderate halophiles. ...

journal_title：Journal of bacteriology

authors： Lee SJ,Lee YJ,Jeong H,Lee SJ,Lee HS,Pan JG,Kim BC,Lee DW

更新日期：2012-11-01 00:00:00

abstract：:A hybrid plasmid was constructed between the 5.3-megadalton plasmid (pUH24) of Anacystis nidulans R2 and the Escherichia coli plasmid pBR322. This was accomplished by adding a transposon to pBR322 and transforming this DNA into A. nidulans. One resultant hybrid, pLS103, had a molecular weight of 6.8 x 10(6), replicate...

journal_title：Journal of bacteriology

authors： Sherman LA,van de Putte P

更新日期：1982-04-01 00:00:00

abstract：:The tprK gene of Treponema pallidum subsp. pallidum, the causative agent of venereal syphilis, belongs to a 12-member gene family and encodes a protein with a predicted cleavable signal sequence and predicted transmembrane domains. Except for the Nichols type strain, all rabbit-propagated isolates of T. pallidum exami...

journal_title：Journal of bacteriology

authors： LaFond RE,Centurion-Lara A,Godornes C,Rompalo AM,Van Voorhis WC,Lukehart SA

更新日期：2003-11-01 00:00:00

abstract：:A gene encoding only the C-terminal portion of the receptor-transducer protein Tar of Escherichia coli was constructed. The gene product was detected and localized in the cytoplasmic fraction of the cell by immunoblotting with anti-Tar antibodies. The C-terminal fragments from wild-type and mutant tar genes were chara...

journal_title：Journal of bacteriology

authors： Oosawa K,Mutoh N,Simon MI

更新日期：1988-06-01 00:00:00

abstract：:The accumulation of imidazoleglycerol phosphate during growth of Neurospora crassa in the presence of 3-amino-1,2,4-triazole was found to cause derepression of tryptophan synthetase and to inhibit the induction of kynureninase. Accumulation of indoleglycerol phosphate in response to growth in the presence of indole ac...

journal_title：Journal of bacteriology

authors： Turner JR,Matchett WH

更新日期：1968-05-01 00:00:00

abstract：:The involvement of the recently characterized 5' exonuclease activity of RNase J1 and endonuclease activity of RNase Y in the turnover of ΔermC mRNA in Bacillus subtilis was investigated. Evidence is presented that both of these activities determine the half-life of ΔermC mRNA. ...

journal_title：Journal of bacteriology

authors： Yao S,Richards J,Belasco JG,Bechhofer DH

更新日期：2011-11-01 00:00:00

abstract：:Three additional ATPase genes, clustered in the order ahaH, ahaI, and ahaK, were found upstream of the previously characterized genes ahaECFABDG coding for the archaeal A1A0 ATPase from Methanosarcina mazei. ahaH, the first gene in the cluster, is preceded by a conserved promoter sequence. Northern blot analysis revea...

journal_title：Journal of bacteriology

authors： Ruppert C,Wimmers S,Lemker T,Müller V

更新日期：1998-07-01 00:00:00

abstract：:The cysteines of the Escherichia coli periplasmic enzyme alkaline phosphatase, which are involved in disulfide bonds in the native enzyme, were found to be fully reduced when the protein was retained in the cytoplasm. Under these circumstances the cysteines remained reduced for at least several minutes after the synth...

journal_title：Journal of bacteriology

authors： Derman AI,Beckwith J

更新日期：1991-12-01 00:00:00

abstract：:The two-partner secretion system ExlBA, expressed by strains of Pseudomonas aeruginosa belonging to the PA7 group, induces hemorrhage in lungs due to disruption of host cellular membranes. Here we demonstrate that the exlBA genes are controlled by a pathway consisting of cAMP and the virulence factor regulator (Vfr). ...

journal_title：Journal of bacteriology

authors： Berry A,Han K,Trouillon J,Robert-Genthon M,Ragno M,Lory S,Attrée I,Elsen S

更新日期：2018-05-24 00:00:00

abstract：:In Escherichia coli, the following genes are involved in motility and chemotaxis. The H gene is the structural gene for flagellin. Mutation in the mot gene results in paralysis of the flagella, and mutation in the fla genes leads to an absence of flagella. The cheA, cheB, and cheC genes are required for chemotaxis. Th...

journal_title：Journal of bacteriology

authors： Armstrong JB,Adler J

更新日期：1969-01-01 00:00:00

abstract：:Nearly every known species of Eubacteria encodes a homolog of the Borrelia burgdorferi EbfC DNA-binding protein. We now demonstrate that fluorescently tagged EbfC associates with B. burgdorferi nucleoids in vivo and that chromatin immunoprecipitation (ChIP) of wild-type EbfC showed it to bind in vivo to sites througho...

journal_title：Journal of bacteriology

authors： Jutras BL,Bowman A,Brissette CA,Adams CA,Verma A,Chenail AM,Stevenson B

更新日期：2012-07-01 00:00:00

abstract：:The Enterococcus faecalis plasmid pAD1 undergoes conjugative transfer in response to cAD1, a peptide sex pheromone emitted by potential bacterial recipients. Regulation of pAD1 transfer involves a number of plasmid-encoded determinants:iad, which determines a peptide-competitive inhibitor iAD1; signal sensing and tran...

journal_title：Journal of bacteriology

authors： Pontius LT,Clewell DB

更新日期：1992-05-01 00:00:00