Abstract:
:Pseudomonas aeruginosa is usually resistant to a wide variety of antibacterial agents, and it has been inferred, on the basis of indirect evidence, that this was due to the low permeability of its outer membrane. We determined the permeability of P. aeruginosa outer membrane directly, by measuring the rates of hydrolysis of cephacetrile, cephaloridine, and various phosphate esters by hydrolytic enzymes located in the periplasm. The permeability to these compounds was about 100-fold lower than in the outer membrane of Escherichia coli K-12. Also, we found that the apparent Km values for active transport of various carbon and energy source compounds were typically higher than 20 microM in P. aeruginosa, in contrast to E. coli in which the values are usually lower than 5 microM. These results also are consistent with the notion that the P. aeruginosa outer membrane indeed has a low permeability to most hydrophilic compounds and that this membrane acts as a rate limiting step in active transport processes with high Vmax values.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Yoshimura F,Nikaido Hsubject
Has Abstractpub_date
1982-11-01 00:00:00pages
636-42issue
2eissn
0021-9193issn
1098-5530journal_volume
152pub_type
杂志文章abstract::We describe here the identification and characterization of two Listeria monocytogenes (Tn917-LTV3) relA and hpt transposon insertion mutants that were impaired in growth after attachment to a model surface. Both mutants were unable to accumulate (p)ppGpp in response to amino acid starvation, whereas the wild-type str...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.3.621-628.2002
更新日期:2002-02-01 00:00:00
abstract::Cryptococcus neoformans is an important pathogenic fungus that has been classified as a basidiomycete. Little is known of the molecular genetics of this fungal pathogen. To begin such studies, we devised a procedure for extraction of DNA from cryptococci; this method involved the use of the cell wall-active enzyme Nov...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.10.5596-5600.1989
更新日期:1989-10-01 00:00:00
abstract::Ishibashi, Masahide (Osaka University, Osaka, Japan), Yoshinobu Sugino, and Yukinori Hirota. Chromosomal location of thymine and arginine genes in Escherichia coli and an F' incorporating them. J. Bacteriol. 87:554-561. 1964.-The gene responsible for thymine requirement or independence in Escherichia coli K-12 is loca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.3.554-561.1964
更新日期:1964-03-01 00:00:00
abstract::Transcriptome analysis of a Bacillus subtilis rok strain that showed reduced complex colony structure formation revealed significant downregulation of the yuaB gene. Overexpression of yuaB restored structure formation in the rok strain. We show that transcription of yuaB is indirectly regulated by Rok, independently f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01170-10
更新日期:2011-02-01 00:00:00
abstract::Rogosa, M. (National Institutes of Health, Bethesda, Md.). The genus Veillonella. I. General cultural, ecological, and biochemical considerations. J. Bacteriol. 87:162-170. 1964.-Arguments are presented for excluding Veillonella discoides, V. reniformis, V. orbiculus, and V. vulvovaginitidis from the genus, and for re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.1.162-170.1964
更新日期:1964-01-01 00:00:00
abstract::Oxygen utilization in splenic cells of normal and BCG-sensitized guinea pigs was measured in a Warburg respirometer in the absence and in the presence of Old Tuberculin (OT). In the absence of OT, normal and sensitive cells respired at the same rate; in the presence of OT, normal cell respiration remained unchanged, w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.1.1-5.1969
更新日期:1969-01-01 00:00:00
abstract::By means of electron microscope autoradiography of component cultures of Bacillus subtilis exposed to [(3)H]thymidine-labeled transforming deoxyribonucleic acid competent and noncompetent cells can be distinguished. Competence is not limited to a specific phase of the cell division cycle. With serial section electron ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.2.334-341.1974
更新日期:1974-05-01 00:00:00
abstract::Mutations of Escherichia coli from sensitivity to nalidixic acid resistance were studied by fluctuation analysis. The mutant distributions in replicate cultures were not significantly affected either by the age of the carbon-starved preculture used for inocula or by the inoculum size. The data from 23 fluctuation test...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.10.2781-2787.1994
更新日期:1994-05-01 00:00:00
abstract::Colicinogenic factors ColI and ColV, which have been shown to behave as sex factors, could not be induced with mitomycin C. In contrast, the ColE(1), ColE(2), and ColE(3) factors, which do not exhibit any fertility factor characteristics, are inducible by this agent. The induced production of colicins E(1), E(2), and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.3.691-699.1967
更新日期:1967-09-01 00:00:00
abstract::The ribG gene at the 5' end of the riboflavin operon of Bacillus subtilis and a reading frame at 442 kb on the Escherichia coli chromosome (subsequently designated ribD) show similarity with deoxycytidylate deaminase and with the RIB7 gene of Saccharomyces cerevisiae. The ribG gene of B. subtilis and the ribD gene of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.6.2022-2028.1997
更新日期:1997-03-01 00:00:00
abstract::Bacteriophage 7-7-1, a member of the family Myoviridae, infects the soil bacterium Agrobacterium sp. strain H13-3. Infection requires attachment to actively rotating bacterial flagellar filaments, with flagellar number, length, and rotation speed being important determinants for infection efficiency. To identify the s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00363-18
更新日期:2018-09-10 00:00:00
abstract::Nitroheterocyclic and nitroaromatic compounds constitute an enormous range of chemicals whose potent biological activity has significant human health and environmental implications. The biological activity of nitro-substituted compounds is derived from reductive metabolism of the nitro moiety, a process catalyzed by a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.21.5529-5539.1998
更新日期:1998-11-01 00:00:00
abstract::From a lysogen with lambda integrated in the leu operon, specialized transducing phages that carry the cell division, murein biosynthesis, and envelope permeability genes located about 0.5 min to the right of leu were isolated. These phages were used to identify the previously undiscovered cell division gene sep. A ge...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.91-100.1978
更新日期:1978-01-01 00:00:00
abstract::The AOX1 gene, which encodes an alternative oxidase, was isolated from the genomic DNA library of Candida albicans. The gene encodes a polypeptide consisting of 379 amino acids with a calculated molecular mass of 43,975 Da. The aox1/aox1 mutant strain did not show cyanide-resistant respiration under normal conditions ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.13.4098-4102.1999
更新日期:1999-07-01 00:00:00
abstract::A hypothesis that preferential rejection of donor markers by the hex system of pneumococcus is due to lethal double-strand breaks has been examined in terms of its implications for the extent of the excision required. Experiments reported here were directed at asking whether hex-dependent marker efficiency depends on ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.1.125-135.1976
更新日期:1976-01-01 00:00:00
abstract::Genome sizes of six different Wolbachia strains from insect and nematode hosts have been determined by pulsed-field gel electrophoresis of purified DNA both before and after digestion with rare-cutting restriction endonucleases. Enzymes SmaI, ApaI, AscI, and FseI cleaved the studied Wolbachia strains at a small number...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.7.2219-2225.2001
更新日期:2001-04-01 00:00:00
abstract::Transcriptional expression of the puc operon in Rhodobacter sphaeroides 2.4.1 is dependent on the partial pressure of oxygen. By using transcriptional fusions in trans of a promoterless fragment derived from the aminoglycoside-3'-phosphotransferase gene of Tn903 to puc operon-specific DNA containing a 629-bp 5' cis-ac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.4.1158-1171.1992
更新日期:1992-02-01 00:00:00
abstract::The acetogenic bacterium Clostridium thermoautotrophicum, grown on methanol, glucose, or CO2-H2, contained high levels of corrinoids, formate dehydrogenase, tetrahydrofolate enzymes, carbon monoxide dehydrogenase, and hydrogenase. Cell-free extracts catalyzed pyruvate-dependent formation of acetate from methyltetrahyd...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.1.507-509.1982
更新日期:1982-07-01 00:00:00
abstract::All Escherichia coli strains so far examined possess a chromosomally encoded nanATEK-yhcH operon for the catabolism of sialic acids. These unique nine-carbon sugars are synthesized primarily by higher eukaryotes and can be used as carbon, nitrogen, and energy sources by a variety of microbial pathogens or commensals. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.16.4806-4815.2003
更新日期:2003-08-01 00:00:00
abstract::Studies of biosynthetic pathways with purines as substrates showed that Methanococcus vannielii was capable of degrading xanthine to an extent that several of the carbon atoms were converted to CO2. Experiments to determine whether this catabolic activity could satisfy the entire nitrogen requirement for growth of M. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.2.681-684.1986
更新日期:1986-08-01 00:00:00
abstract::Unsaturated fatty acid (ufa) auxotrophs of Neurospora crassa were obtained by treatment of conidia with N-methyl-N'-nitro-N-nitrosoguanidine followed by isolation on media containing polyunsaturated fatty acids suspended in Tergitol NP-40. The 24 mutants for which reisolates were obtained from crosses with wild type w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.3.1144-1148.1977
更新日期:1977-06-01 00:00:00
abstract::The so-called symbiotic region of the Bradyrhizobium japonicum chromosome (C. Kündig, H. Hennecke, and M. Göttfert, J. Bacteriol. 175:613-622, 1993) was screened for the presence of genes controlled by the nitrogen fixation regulatory protein NifA. Southern blots of restriction enzyme-digested cosmids that represent a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.6.1472-1480.2000
更新日期:2000-03-01 00:00:00
abstract::Western immunoblots and assays of Bacillus subtilis extracts showed that intracellular serine protease 1 is produced in a form larger than previously reported, appears not to have undergone N-terminal processing, and is active in the presence or absence of calcium. No evidence for an inactive precursor form of the pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.1.473-476.1990
更新日期:1990-01-01 00:00:00
abstract::Clostridium sporogenes PA 3679 is widely used as a nontoxigenic surrogate for proteolytic strains of Clostridium botulinum in the derivation and validation of thermal processes in food. Here we report the draft assembly and annotation of the C. sporogenes PA 3679 genome. Preliminary analysis demonstrates a high degree...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06765-11
更新日期:2012-03-01 00:00:00
abstract::The Tat system is a recently discovered protein export pathway that serves to translocate folded proteins, often containing redox cofactors, across the bacterial cytoplasmic membrane. Here we report that tat strains are associated with a mutant cell septation phenotype, where chains of up to 10 cells are evident. Muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.1.139-144.2001
更新日期:2001-01-01 00:00:00
abstract::Pyrrolnitrin at 10 mug/ml inhibited the growth of Saccharomyces cerevisiae, Penicillium atrovenetum, and P. oxalicum. The primary site of action of pyrrolnitrin on S. cerevisiae was the terminal electron transport system between succinate or reduced nicotinamide adenine dinucleotide (NADH) and coenzyme Q. At growth in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.310-318.1969
更新日期:1969-10-01 00:00:00
abstract::The specificity of the growth requirement of Methanobacterium ruminantium strain M1 for a new coenzyme, 2-mercaptoethanesulfonic acid (HS--CoM), was examined. A variety of derivatives, analogs, and potential biosynthetic precursors of coenzyme M were tested; only a restricted range of thioether, thioester, and thiocar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.1.256-263.1979
更新日期:1979-01-01 00:00:00
abstract::Exogenous proline specifically stimulates the growth rate of enteric bacteria in media of inhibitory osmotic strength (J. H. B. Christian, Aust. J. Biol. Sci. 8:490-497, 1955). I observed that Salmonella typhimurium mutants which lack both of the previously known proline permeases (putP proP) are stimulated by proline...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.3.1433-1443.1982
更新日期:1982-09-01 00:00:00
abstract::Stalk formation is a novel pattern of multicellular organization. Yeast cells which survive UV irradiation form colonies that grow vertically to form very long (0.5 to 3.0 cm) and thin (0.5 to 4 mm in diameter) multicellular structures. We describe the conditions required to obtain these stalk-like structures reproduc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.15.3992-3996.1998
更新日期:1998-08-01 00:00:00
abstract::Among strains of Haemophilus influenzae, the ability to catabolize tryptophan (as detected by indole production) varies and is correlated with pathogenicity. Tryptophan catabolism is widespread (70 to 75%) among harmless respiratory isolates but is nearly universal (94 to 100%) among strains causing serious disease, i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.1.107-118.1998
更新日期:1998-01-01 00:00:00