Abstract:
:The genes governing three of the enzymes of the isoleucine-valine biosynthetic pathway form the operon: operator-ilvA-ilvD-ilvE. The enzymes are: ilvA, l-threonine deaminase; ilvD, dihydroxy acid dehydrase; and ilvE, transaminase B. A nonsense mutation in the ilvD gene (D-ochre) and a nonsense mutation in the ilvE gene (E-amber) affect the properties of the proximal gene product, l-threonine deaminase (TD), in addition to inactivating the enzymes produced by the genes in which the mutations have occurred. The D-ochre mutation causes TD to move in diffusion and gel filtration experiments as though it were 30% smaller than the wild-type enzyme. The E-amber mutation causes TD to move in similar experiments as though it were much larger than the wild-type enzyme. Both mutations completely abolish the sensitivity of TD to l-isoleucine, the normal feedback inhibitor of the wild-type enzyme. The effects of the nonsense mutations on TD can be reversed in three ways: by genetic reversion of the D-ochre mutation; by treatment of the altered enzymes with 3.0 m urea; and by forming a heterozygous diploid, containing the wild-type allele as well as the mutant allele of ilvD or ilvE. The results suggest that the subunits of TD undergo abnormal aggregation in the presence of the partial polypeptides produced by the mutant alleles of ilvD or ilvE; multi-enzyme aggregates in extracts of wild type, however, could not be detected.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Wechsler JA,Adelberg EAdoi
10.1128/JB.98.3.1179-1194.1969subject
Has Abstractpub_date
1969-06-01 00:00:00pages
1179-94issue
3eissn
0021-9193issn
1098-5530journal_volume
98pub_type
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journal_title:Journal of bacteriology
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doi:10.1128/JB.117.2.900-903.1974
更新日期:1974-02-01 00:00:00
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更新日期:2004-11-01 00:00:00
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更新日期:1986-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.1.234-239.1970
更新日期:1970-04-01 00:00:00
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更新日期:2004-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1987-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.3.1171-1174.1982
更新日期:1982-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.3.968-977.1972
更新日期:1972-06-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/jb.176.24.7653-7658.1994
更新日期:1994-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.1.74-80.1968
更新日期:1968-01-01 00:00:00
abstract::The complete Bacillus subtilis genome contains two genes with the potential to encode glutamate dehydrogenase (GlutDH) enzymes. Mutations in these genes were constructed and characterized. The rocG gene proved to encode a major GlutDH whose synthesis was induced in media containing arginine or ornithine or, to a lesse...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1996-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1963-09-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1994-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.175.24.8018-8023.1993
更新日期:1993-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1973-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1968-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.4.860-866.1962
更新日期:1962-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.19.5898-5908.1999
更新日期:1999-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3853-3856.1987
更新日期:1987-08-01 00:00:00