Lactate dehydrogenase activity in certain strains of Staphylococcus aureus.

abstract：:Protein export in Escherichia coli is mediated by translocase, a multisubunit membrane protein complex with SecA as the peripheral subunit and the SecY, SecE, and SecG proteins as the integral membrane domain. In the gram-positive bacterium Bacillus subtilis, SecA, SecY, and SecE have been identified through genetic a...

journal_title：Journal of bacteriology

authors： van Wely KH,Swaving J,Broekhuizen CP,Rose M,Quax WJ,Driessen AJ

更新日期：1999-03-01 00:00:00

abstract：:We have previously shown that both ends of the Tn3 family transposon Tn4652 contain integration host factor (IHF) binding sites and that IHF positively regulates expression of the Tn4652 transposase gene tnpA in Pseudomonas putida (R. Hõrak, and M. Kivisaar, J. Bacteriol. 180:2822-2829, 1998). Tn4652 can activate sile...

journal_title：Journal of bacteriology

authors： Teras R,Hõrak R,Kivisaar M

更新日期：2000-02-01 00:00:00

abstract：:To determine whether homologous recombination could be used to inactivate selected genes in Spiroplasma citri, plasmid constructs were designed to disrupt the motility gene scm1. An internal scm1 gene fragment was inserted into plasmid pKT1, which replicates in Escherichia coli but not in S. citri, and into the S. cit...

journal_title：Journal of bacteriology

authors： Duret S,Danet JL,Garnier M,Renaudin J

更新日期：1999-12-01 00:00:00

abstract：:Those genes in Escherichia coli defined by mutations which result in an inability to grow at high temperatures are designated htr, indicating a high temperature requirement. A new htr mutant of E. coli was isolated and characterized and is designated htrD. The htrD gene has been mapped to 19.3 min on the E. coli chrom...

journal_title：Journal of bacteriology

authors： Delaney JM,Ang D,Georgopoulos C

更新日期：1992-02-01 00:00:00

abstract：:The photosynthetic cyanobacterium Synechocystis sp. strain PCC6803 possesses homologs of known genes of the non-mevalonate 2-C-methyl-D-erythritol 2-phosphate (MEP) pathway for synthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Isoprenoid biosynthesis in extracts of this cyanobacterium, ...

journal_title：Journal of bacteriology

authors： Ershov YV,Gantt RR,Cunningham FX Jr,Gantt E

更新日期：2002-09-01 00:00:00

abstract：:Mutations in the spoIIIA and spoIIIJ loci disrupt the compartmentalization of gene expression during sporulation of Bacillus subtilis. The breakdown in compartmentalization is not the cause of their being blocked in spore formation. Rather, it appears to be a consequence of the engulfed prespore's being unstable. ...

journal_title：Journal of bacteriology

authors： Li Z,Di Donato F,Piggot PJ

更新日期：2004-04-01 00:00:00

abstract：:Nisin is a pore-forming antimicrobial peptide. The capacity of nisin to induce transmembrane movement of a fluorescent phospholipid in lipid vesicles was investigated. Unilamellar phospholipid vesicles that contained a fluorescent phospholipid (1-acyl-2-(6-[(7-nitro-2-1, 3-benzoxadiazol-4-yl)amino]caproyl)-sn-glycero-...

journal_title：Journal of bacteriology

authors： Moll GN,Konings WN,Driessen AJ

更新日期：1998-12-01 00:00:00

abstract：:Agrobacterium radiobacter NCIB 11883 was grown in lactose-limited continuous culture at a dilution rate of 0.045/h. Washed cells transported [14C]lactose and [methyl-14C]beta-D-thiogalactoside, a nonmetabolisable analog of lactose, at similar rates and with similar affinities (Km for transport, less than 1 microM). Tr...

journal_title：Journal of bacteriology

authors： Greenwood JA,Cornish A,Jones CW

更新日期：1990-04-01 00:00:00

abstract：:Pseudomonas aeruginosa XMG, isolated from soil, utilizes lactate. Here we present a 6.45-Mb assembly of its genome sequence. Besides the lactate utilization mechanism of the strain, the genome sequence may also provide other useful information related to P. aeruginosa, such as identifying genes involved in virulence, ...

journal_title：Journal of bacteriology

authors： Gao C,Hu C,Ma C,Su F,Yu H,Jiang T,Dou P,Wang Y,Qin T,Lv M,Xu P

更新日期：2012-09-01 00:00:00

abstract：:The heptapeptide-nucleotide microcin C (McC) targets aspartyl-tRNA synthetase. Upon its entry into a susceptible cell, McC is processed to release a nonhydrolyzable aspartyl-adenylate that inhibits aspartyl-tRNA synthetase, leading to the cessation of translation and cell growth. Here, we surveyed Escherichia coli cel...

journal_title：Journal of bacteriology

authors： Kazakov T,Vondenhoff GH,Datsenko KA,Novikova M,Metlitskaya A,Wanner BL,Severinov K

更新日期：2008-04-01 00:00:00

abstract：:We report on the cloning and characterization of the rfb gene cluster of the O75 lipopolysaccharide from a urinary tract isolate of Escherichia coli. Deletion cloning defined the minimum region of DNA that expressed the O75 antigen in E. coli host strains to be on a 12.4-kb insert. However, the E. coli strain expressi...

journal_title：Journal of bacteriology

authors： Batchelor RA,Haraguchi GE,Hull RA,Hull SI

更新日期：1991-09-01 00:00:00

abstract：:Rhodobacter sphaeroides is a free-living, photoheterotrophic bacterium known for its genomic and metabolic complexity. We have discovered that this purple photosynthetic organism possesses a quorum-sensing system. Quorum sensing occurs in a number of eukaryotic host-associated gram-negative bacteria. In these bacteria...

journal_title：Journal of bacteriology

authors： Puskas A,Greenberg EP,Kaplan S,Schaefer AL

更新日期：1997-12-01 00:00:00

abstract：:Bacillus subtilis mutants with reduced rates of cell wall autolysis reached a constant rate of wall turnover after a longer lag than the standard strain but eventually showed the same turnover rate. In reverse, a turnover-deficient mutant autolysed at a slightly higher rate than the standard strain. Consequently, ther...

journal_title：Journal of bacteriology

authors： Vitković L,Cheung HY,Freese E

更新日期：1984-01-01 00:00:00

abstract：:Fast-growing species of Rhizobium were found to secrete low-molecular-weight beta-2-linked glucans when cultured in synthetic liquid medium. These glucans are quite similar to beta-2-linked glucans produced by species of Agrobacterium. No reducing terminus was detected in these glucans. ...

journal_title：Journal of bacteriology

authors： York WS,McNeil M,Darvill AG,Albersheim P

更新日期：1980-04-01 00:00:00

abstract：:The activities of DNA methyltransferases are important for a variety of cellular functions in bacteria. In this study, we developed a modified high-throughput technique called methyl homopolymer tail mediated sequencing (methyl HTM-seq) to identify the undermethylated sites in the Vibrio cholerae genome for the two DN...

journal_title：Journal of bacteriology

authors： Dalia AB,Lazinski DW,Camilli A

更新日期：2013-05-01 00:00:00

abstract：:Primer extension analysis of transcripts of the Bacillus subtilis autolysin (cwlB) operon indicated that SigD-dependent transcripts from the Pd promoter are missing in the degU32(Hy) and degS200 (Hy) mutants. The degU32(Hy) mutation caused a 99% reduction in the expression of a sigD-lacZ translational fusion gene cons...

journal_title：Journal of bacteriology

authors： Tokunaga T,Rashid MH,Kuroda A,Sekiguchi J

更新日期：1994-08-01 00:00:00

abstract：:Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.), and Guy P. Youmans. Immunogenic activity of a ribosomal fraction obtained from Mycobacterium tuberculosis. J. Bacteriol. 89:1291-1298. 1965.-The highly immunogenic particulate fraction obtained from mechanically ruptured cells of the H37Ra strai...

journal_title：Journal of bacteriology

authors： YOUMANS AS,YOUMANS GP

更新日期：1965-05-01 00:00:00

abstract：:The R-factor R1 is present in a low number of copies per genome (near unity, so-called stringent control of replication). The replication of R1 was studied in a density-shift experiment. One generation after the shift about 20% of the R1 copies had not replicated, whereas about 20% had replicated at least twice. The r...

journal_title：Journal of bacteriology

authors： Gustafsson P,Nordström K

更新日期：1975-08-01 00:00:00

abstract：:The tprK gene of Treponema pallidum subsp. pallidum, the causative agent of venereal syphilis, belongs to a 12-member gene family and encodes a protein with a predicted cleavable signal sequence and predicted transmembrane domains. Except for the Nichols type strain, all rabbit-propagated isolates of T. pallidum exami...

journal_title：Journal of bacteriology

authors： LaFond RE,Centurion-Lara A,Godornes C,Rompalo AM,Van Voorhis WC,Lukehart SA

更新日期：2003-11-01 00:00:00

abstract：:Penicillin-binding protein 2 (PBP-2) of Escherichia coli K-12 was purified by covalent affinity chromatography using 6-aminopenicillanic acid covalently coupled to carboxymethyl-Sepharose (6-APA-CM-Sepharose). Purification of PBP-2 was accomplished by prebinding the methoxy cephalosporin, cefoxitin, to the Triton X-10...

journal_title：Journal of bacteriology

authors： Curtis SJ,Strominger JL

更新日期：1981-01-01 00:00:00

abstract：:The alkaline phosphatase (orthophosphoric monoester phosphydrolase, EC 3.1.3.1) of Bacillus licheniformis MC14 was studied in an attempt to determine the number of subunits contained in the 120,000-molecular-weight native enzyme. Two moles of arginine was liberated per mole of native enzyme by carboxypeptidases A and ...

journal_title：Journal of bacteriology

authors： Hulett FM,Schaffel SD,Campbell LL

更新日期：1976-11-01 00:00:00

abstract：:Microarray studies of the Escherichia coli response to nitric oxide and nitrosative stress have suggested that additional transcriptional regulators of this response remain to be characterized. We identify here the product of the yjeB gene as a negative regulator of the transcription of the ytfE, hmpA and ygbA genes, ...

journal_title：Journal of bacteriology

authors： Bodenmiller DM,Spiro S

更新日期：2006-02-01 00:00:00

abstract：:The cobB function of Salmonella typhimurium LT2 was defined in vivo as an alternative activity for the nicotinic acid mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase enzyme (CobT), which is involved in the assembly of the nucleotide loop of cobalamin in this bacterium (J. R. Trzebiatowski, G. A. O'T...

journal_title：Journal of bacteriology

authors： Tsang AW,Escalante-Semerena JC

更新日期：1996-12-01 00:00:00

abstract：:A superfamily of transport proteins, which includes the lactose permease of Escherichia coli, contains a highly conserved motif, G-X-X-X-D/E-R/K-X-G-R/K-R/K, in the loops that connect transmembrane segments 2 and 3 and transmembrane segments 8 and 9. Previous analysis of this motif in the lactose permease (A. E. Jesse...

journal_title：Journal of bacteriology

authors： Jessen-Marshall AE,Parker NJ,Brooker RJ

更新日期：1997-04-01 00:00:00

abstract：:Escherichia coli mutants with improved organic solvent tolerance levels showed high levels of outer membrane protein TolC and inner membrane protein AcrA. The TolC level was regulated positively by MarA, Rob, or SoxS. A possible mar-rob-sox box sequence was found upstream of the tolC gene. These findings suggest that ...

journal_title：Journal of bacteriology

authors： Aono R,Tsukagoshi N,Yamamoto M

更新日期：1998-02-01 00:00:00

abstract：:The colicin E2 immunity (ceiB) and lysis (celB) genes of colicin plasmid ColE2-P9 were cloned as a 900-base-pair insert under the control of the lac promoter in high-copy-number plasmid pUR222. Hosts carrying this plasmid were immune to colicin E2, produced increased amounts of immunity protein (molecular weight, 9,00...

journal_title：Journal of bacteriology

authors： Pugsley AP,Schwartz M

更新日期：1983-10-01 00:00:00

abstract：:Mevalonate (MVA) metabolism provides the isoprenoids used in archaeal lipid biosynthesis. In synthesis of isopentenyl diphosphate, the classical MVA pathway involves decarboxylation of mevalonate diphosphate, while an alternate pathway has been proposed to involve decarboxylation of mevalonate monophosphate. To identi...

journal_title：Journal of bacteriology

authors： Vannice JC,Skaff DA,Keightley A,Addo JK,Wyckoff GJ,Miziorko HM

更新日期：2014-03-01 00:00:00

abstract：:Streptococcus agalactiae is a primary cause of neonatal morbidity and mortality. Essential to the virulence of this pathogen is the production of a type-specific capsular polysaccharide (CPS) that enables the bacteria to evade host immune defenses. The identification, cloning, sequencing, and functional characterizati...

journal_title：Journal of bacteriology

authors： Chaffin DO,Beres SB,Yim HH,Rubens CE

更新日期：2000-08-01 00:00:00

abstract：:Integrating conjugative elements (ICEs) of the SXT/R391 family are major contributors to the spread of antibiotic resistance genes. These elements also catalyze their own diversity by promoting inter-ICE recombination through the action of the RecA-independent homologous recombination system that they encode. Here, we...

journal_title：Journal of bacteriology

authors： Garriss G,Poulin-Laprade D,Burrus V

更新日期：2013-05-01 00:00:00

abstract：:Bam35, a temperate double-stranded DNA bacteriophage with a 15-kb linear genome, infects gram-positive Bacillus thuringiensis cells. Bam35 morphology and genome organization resemble those of PRD1, a lytic phage infecting gram-negative bacteria. Bam35 and PRD1 have an outer protein coat surrounding a membrane that enc...

journal_title：Journal of bacteriology

authors： Gaidelyte A,Jaatinen ST,Daugelavicius R,Bamford JK,Bamford DH

更新日期：2005-05-01 00:00:00