Regulation by a novel protein of the bimodal distribution of lipopolysaccharide in the outer membrane of Escherichia coli.

abstract：:The flagella of Methanococcus voltae were isolated by using three procedures. Initially, cells were sheared to release the filaments, which were purified by differential centrifugation and banding in KBr gradients. Flagella were also prepared by solubilization of cells with 1% (vol/vol) Triton X-100 and purified as de...

journal_title：Journal of bacteriology

authors： Kalmokoff ML,Jarrell KF,Koval SF

更新日期：1988-04-01 00:00:00

abstract：:Transcriptome analysis of a Bacillus subtilis rok strain that showed reduced complex colony structure formation revealed significant downregulation of the yuaB gene. Overexpression of yuaB restored structure formation in the rok strain. We show that transcription of yuaB is indirectly regulated by Rok, independently f...

journal_title：Journal of bacteriology

authors： Kovács AT,Kuipers OP

更新日期：2011-02-01 00:00:00

abstract：:The nucleotide messenger cyclic di-GMP (c-di-GMP) plays a central role in the regulation of motility, virulence, and biofilm formation in many pathogenic bacteria. EAL domain-containing phosphodiesterases are the major signaling proteins responsible for the degradation of c-di-GMP and maintenance of its cellular level...

journal_title：Journal of bacteriology

authors： Chen MW,Kotaka M,Vonrhein C,Bricogne G,Rao F,Chuah ML,Svergun D,Schneider G,Liang ZX,Lescar J

更新日期：2012-09-01 00:00:00

abstract：:Multiple copies of a gene require enhanced investment on the part of the cell and, as such, call for an explanation. The observation that Escherichia coli has four copies of initiator tRNA (tRNAi) genes, encoding a special tRNA (tRNA(fMet)) required to start protein synthesis, is puzzling particularly because the cell...

journal_title：Journal of bacteriology

authors： Samhita L,Nanjundiah V,Varshney U

更新日期：2014-07-01 00:00:00

abstract：:wbdA is a mannosyltransferase gene that is involved in synthesis of the Escherichia coli O9a polysaccharide, a mannose homopolymer with a repeating unit of 2-alphaMan-1,2-alphaMan-1,3-alphaMan-1, 3-alphaMan-1. The equivalent structural O polysaccharide in the E. coli O9 and Klebsiella O3 strains is 2-alphaMan-1,2-alph...

journal_title：Journal of bacteriology

authors： Kido N,Kobayashi H

更新日期：2000-05-01 00:00:00

abstract：:Tetragenococcus halophila D10 catalyzes the decarboxylation of L-aspartate with nearly stoichiometric release of L-alanine and CO(2). This trait is encoded on a 25-kb plasmid, pD1. We found in this plasmid a putative asp operon consisting of two genes, which we designated aspD and aspT, encoding an L-aspartate-beta-de...

journal_title：Journal of bacteriology

authors： Abe K,Ohnishi F,Yagi K,Nakajima T,Higuchi T,Sano M,Machida M,Sarker RI,Maloney PC

更新日期：2002-06-01 00:00:00

abstract：:We characterized three distinct families of repeated sequences in the genome of the cyanobacterium Calothrix sp. strain PCC 7601. These repeated sequences were present at a level of about 100 copies per Calothrix genome and consisted of tandemly amplified heptanucleotides. These elements were named short tandemly repe...

journal_title：Journal of bacteriology

authors： Mazel D,Houmard J,Castets AM,Tandeau de Marsac N

更新日期：1990-05-01 00:00:00

abstract：:The proteome of Haemophilus influenzae strain Rd KW20 was analyzed by liquid chromatography (LC) coupled with ion trap tandem mass spectrometry (MS/MS). This approach does not require a gel electrophoresis step and provides a rapidly developed snapshot of the proteome. In order to gain insight into the central metabol...

journal_title：Journal of bacteriology

authors： Kolker E,Purvine S,Galperin MY,Stolyar S,Goodlett DR,Nesvizhskii AI,Keller A,Xie T,Eng JK,Yi E,Hood L,Picone AF,Cherny T,Tjaden BC,Siegel AF,Reilly TJ,Makarova KS,Palsson BO,Smith AL

更新日期：2003-08-01 00:00:00

abstract：:The uhp locus of Escherichia coli contains genes for the sugar phosphate transport system (uhpT) and the regulatory system which allows its induction by external glucose 6-phosphate (uhpRA). The uhp region was cloned onto high-copy-number plasmids, both from Uhp(+) plasmids of the Clarke-Carbon collection and from gen...

journal_title：Journal of bacteriology

authors： Shattuck-Eidens DM,Kadner RJ

更新日期：1983-09-01 00:00:00

abstract：:Protein synthesis in Allomyces marcogynus mitospores began at the time of encystment and was required for further germination. The rate of protein synthesis in germinating spores decreased in the absence of ribonucleic acid synthesis relative to the uninhibited control. There was a concomitant decrease in polysome con...

journal_title：Journal of bacteriology

authors： Smith BA,Burke DD

更新日期：1980-09-01 00:00:00

abstract：:The rnt gene encoding RNase T was cloned on a 13-kilobase BamHI fragment. Restriction analysis of the fragment and comparison of it with the Escherichia coli restriction map localized rnt to kilobase coordinates 1733 to 1746, corresponding to about 36 min on the genetic map. The map location was confirmed by cotransdu...

journal_title：Journal of bacteriology

authors： Case LM,Chen XN,Deutscher MP

更新日期：1989-10-01 00:00:00

abstract：:A new insertion element, IS1549, was identified serendipitously from Mycobacterium smegmatis LR222 during experiments using a vector designed to detect the excision of IS6110 from between the promoter region and open reading frame (ORF) of an aminoglycoside phosphotransferase gene. Six of the kanamycin-resistant isola...

journal_title：Journal of bacteriology

authors： Plikaytis BB,Crawford JT,Shinnick TM

更新日期：1998-03-01 00:00:00

abstract：:In contrast to previous findings, we demonstrate that the dissimilatory (bi)sulfite reductase genes (dsrAB) of Desulfobacula toluolica were vertically inherited. Furthermore, Desulfobacterium anilini and strain mXyS1 were identified, by dsrAB sequencing of 17 reference strains, as members of the donor lineage for thos...

journal_title：Journal of bacteriology

authors： Zverlov V,Klein M,Lücker S,Friedrich MW,Kellermann J,Stahl DA,Loy A,Wagner M

更新日期：2005-03-01 00:00:00

abstract：:Rhodobacter sphaeroides is a free-living, photoheterotrophic bacterium known for its genomic and metabolic complexity. We have discovered that this purple photosynthetic organism possesses a quorum-sensing system. Quorum sensing occurs in a number of eukaryotic host-associated gram-negative bacteria. In these bacteria...

journal_title：Journal of bacteriology

authors： Puskas A,Greenberg EP,Kaplan S,Schaefer AL

更新日期：1997-12-01 00:00:00

abstract：:Mouse-virulent Salmonella typhimurium strains SR-11 and ATCC 14028-1s express curli fibers, thin aggregative fibers, at ambient temperature on plates as judged by Western blot analysis and electron microscopy. Concomitantly with curli expression, cells develop a rough and dry colony morphology and bind the dye Congo r...

journal_title：Journal of bacteriology

authors： Römling U,Bian Z,Hammar M,Sierralta WD,Normark S

更新日期：1998-02-01 00:00:00

abstract：:Pseudomonas fluorescens strain KU-7 is a prototype microorganism that metabolizes 2-nitrobenzoate (2-NBA) via the formation of 3-hydroxyanthranilate (3-HAA), a known antioxidant and reductant. The initial two steps leading to the sequential formation of 2-hydroxy/aminobenzoate and 3-HAA are catalyzed by a NADPH-depend...

journal_title：Journal of bacteriology

authors： Iwaki H,Muraki T,Ishihara S,Hasegawa Y,Rankin KN,Sulea T,Boyd J,Lau PC

更新日期：2007-05-01 00:00:00

abstract：:Members of the Streptococcus bovis group are important causes of endocarditis. However, factors associated with their pathogenicity, such as adhesins, remain uncharacterized. We recently demonstrated that endocarditis-derived Streptococcus gallolyticus subsp. gallolyticus isolates frequently adhere to extracellular ma...

journal_title：Journal of bacteriology

authors： Sillanpää J,Nallapareddy SR,Qin X,Singh KV,Muzny DM,Kovar CL,Nazareth LV,Gibbs RA,Ferraro MJ,Steckelberg JM,Weinstock GM,Murray BE

更新日期：2009-11-01 00:00:00

abstract：:The interaction between endonuclease V, the cyclobutane pyrimidine dimer-specific N-glycosylase/abasic lyase from bacteriophage T4, and DNA was investigated by DNase I footprinting methods. The catalytically inactive mutant E23Q was found to interact with a smaller region of DNA at the abasic site analog, tetrahydrofu...

journal_title：Journal of bacteriology

authors： Latham KA,Manuel RC,Lloyd RS

更新日期：1995-09-01 00:00:00

abstract：:A DNA region carrying lysS, the gene encoding the lysyl-tRNA synthetase, was cloned from the extreme thermophile prokaryote Thermus thermophilus VK-1 and sequenced. The analysis indicated an open reading frame encoding a protein of 492 amino acids. This putative protein has significant homologies to previously sequenc...

journal_title：Journal of bacteriology

authors： Chen J,Brevet A,Lapadat-Tapolsky M,Blanquet S,Plateau P

更新日期：1994-05-01 00:00:00

abstract：:A thermosensitive sporulation mutant of Bacillus subtilis containing a mutation in the secY gene was isolated and characterized. No asymmetric septum specific to the sporulation was detected by electron microscopy at the nonpermissive temperature, indicating that the block occurred at a very early stage of sporulation...

journal_title：Journal of bacteriology

authors： Yoshikawa H,Jeong SM,Hirata A,Kawamura F,Doi RH,Takahashi H

更新日期：1993-06-01 00:00:00

abstract：:Osmotic stress was found to induce biofilm formation in a Staphylococcus aureus mucosal isolate. Inactivation of a global regulator of the bacterial stress response, the alternative transcription factor sigma(B), resulted in a biofilm-negative phenotype and loss of salt-induced biofilm production. Complementation of t...

journal_title：Journal of bacteriology

authors： Rachid S,Ohlsen K,Wallner U,Hacker J,Hecker M,Ziebuhr W

更新日期：2000-12-01 00:00:00

abstract：:The E. coli RelA protein is a ribosome-dependent (p)ppGpp synthetase that is activated in response to amino acid starvation. RelA can be dissected both functionally and physically into two domains: The N-terminal domain (NTD) (amino acids [aa] 1 to 455) contains the catalytic domain of RelA, and the C-terminal domain ...

journal_title：Journal of bacteriology

authors： Gropp M,Strausz Y,Gross M,Glaser G

更新日期：2001-01-01 00:00:00

abstract：:Integral membrane proteins from over 20 ubiquitous families of channels, secondary carriers, and primary active transporters were analyzed for average size differences between homologues from the three domains of life: Bacteria, Archaea, and Eucarya. The results showed that while eucaryotic homologues are consistently...

journal_title：Journal of bacteriology

authors： Chung YJ,Krueger C,Metzgar D,Saier MH Jr

更新日期：2001-02-01 00:00:00

abstract：:The composition of the molecular species of acidic phospholipids in Escherichia coli B during the late exponential growth phase at 37 degrees C was determined. Two phosphatidyl groups of cardiolipin, the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties of cardiolipin, were isolated by limited hydrolysis with p...

journal_title：Journal of bacteriology

authors： Yokota K,Kanamoto R,Kito M

更新日期：1980-03-01 00:00:00

abstract：:Acid titrations of intact and butanol-treated cells of Streptococcus faecalis revealed that nearly all of the intracellular K(+) ions could diffuse into the suspending medium in association with small anions, including ribonucleic acid breakdown products, when the cell membrane was damaged. In contrast, nearly all of ...

journal_title：Journal of bacteriology

authors： Marquis RE,Porterfield N,Matsumura P

更新日期：1973-05-01 00:00:00

abstract：:As a result of serial exposures to a mutagenic agent, N-methyl-N'-nitro-N-nitrosoguanidine, the yield of enterotoxin A produced by the last mutant in the series was increased nearly 20-fold over the amount produced by the parent Staphylococcus aureus 100. ...

journal_title：Journal of bacteriology

authors： Friedman ME,Howard MB

更新日期：1971-04-01 00:00:00

abstract：:Pfs expression is required for several metabolic pathways and limits the production of autoinducer-2, a molecule proposed to play a central role in interspecies quorum sensing. The present study reveals physiological conditions and promoter DNA elements that regulate Escherichia coli pfs transcription. Pfs transcripti...

journal_title：Journal of bacteriology

authors： Kim Y,Lew CM,Gralla JD

更新日期：2006-11-01 00:00:00

abstract：:MotA contains a conserved C-terminal cluster of negatively charged residues, and MotB contains a conserved N-terminal cluster of positively charged residues. Charge-altering mutations affecting these residues impair motility but do not diminish Mot protein levels. The motility defects are reversed by second-site mutat...

journal_title：Journal of bacteriology

authors： Hosking ER,Manson MD

更新日期：2008-08-01 00:00:00

abstract：:Alcaligenes eutrophus H16 produces two [NiFe] hydrogenases which catalyze the oxidation of hydrogen and enable the organism to utilize H2 as the sole energy source. The genes (hoxK and hoxG) for the heterodimeric, membrane-bound hydrogenase (MBH) are located adjacent to a series of eight accessory genes (hoxZ, hoxM, h...

journal_title：Journal of bacteriology

authors： Bernhard M,Schwartz E,Rietdorf J,Friedrich B

更新日期：1996-08-01 00:00:00

abstract：:The RNA polymerase (RNAP) binding protein A (RbpA) contributes to the formation of stable RNAP-promoter open complexes (RPo) and is essential for viability in mycobacteria. Four domains have been identified in the RbpA protein, i.e., an N-terminal tail (NTT) that interacts with RNAP β' and σ subunits, a core domain (C...

journal_title：Journal of bacteriology

authors： Prusa J,Jensen D,Santiago-Collazo G,Pope SS,Garner AL,Miller JJ,Ruiz Manzano A,Galburt EA,Stallings CL

更新日期：2018-06-11 00:00:00