A single amino acid substitution in a mannosyltransferase, WbdA, converts the Escherichia coli O9 polysaccharide into O9a: generation of a new O-serotype group.

abstract：:High-affinity iron acquisition in Bacillus subtilis is mediated via the bacillibactin catechole siderophore pathway. Three of the four essential pathway steps, bacillibactin synthesis, Fe-bacillibactin uptake, and Fe-bacillibactin hydrolysis have been characterized previously. The functional and regulatory components ...

journal_title：Journal of bacteriology

authors： Miethke M,Schmidt S,Marahiel MA

更新日期：2008-08-01 00:00:00

abstract：:The cell walls of a selected isolate of Staphylococcus aureus FDA 209P were observed undergoing progressive disintegration when exposed to lysostaphin (1 unit/ml) in 24% NaCl solution. Electron micrographs of ultrathin sections of test cells after exposure to lysostaphin for 2 min showed only superficial evidence of l...

journal_title：Journal of bacteriology

authors： Schuhardt VT,Huber TW,Pope LM

更新日期：1969-01-01 00:00:00

abstract：:Levin, Alfred P. (The Johns Hopkins University, Baltimore, Md.), and Philip E. Hartman. Action of a histidine analogue, 1,2,4-triazole-3-alanine, in Salmonella typhimurium. J. Bacteriol. 86:820-828. 1963.-The effect of the histidine analogue, 1,2,4-triazole-3-alanine (TRA), on growth and enzyme synthesis in histidine ...

journal_title：Journal of bacteriology

authors： LEVIN AP,HARTMAN PE

更新日期：1963-10-01 00:00:00

abstract：:Proteins of the YidC/OxaI/Alb3 family play a crucial role in the insertion, folding, and/or assembly of membrane proteins in prokaryotes and eukaryotes. Bacillus subtilis has two YidC-like proteins, denoted SpoIIIJ and YqjG. SpoIIIJ and YqjG are largely exchangeable in function, but SpoIIIJ has a unique role in sporul...

journal_title：Journal of bacteriology

authors： Geng Y,de Keyzer J,Scheffers DJ,Driessen AJ

更新日期：2014-04-01 00:00:00

abstract：:In Pseudomonas aeruginosa, flagellar genes are regulated in a cascade headed by FleQ, an NtrC/NifA-type activator. FleQ and RpoN positively regulate expression of flhA, fliE, fliL, and fleSR genes, among others. Direct interaction of FleQ with flhA, fliE, fliL, and fleSR promoters was demonstrated by gel shift assay, ...

journal_title：Journal of bacteriology

authors： Jyot J,Dasgupta N,Ramphal R

更新日期：2002-10-01 00:00:00

abstract：:Xylella fastidiosa is a phytopathogenic bacterium that is responsible for diseases in many economically important crops. Although different strains have been studied, little is known about X. fastidiosa stress responses. One of the better characterized stress responses in bacteria is the heat shock response, which ind...

journal_title：Journal of bacteriology

authors： Koide T,Vêncio RZ,Gomes SL

更新日期：2006-08-01 00:00:00

abstract：:The ruvB genes of the highly divergent thermophilic eubacteria Thermus thermophilus and Thermotoga maritima were cloned, sequenced, and expressed in Escherichia coli. Both thermostable RuvB proteins were purified to homogeneity. Like E. coli RuvB protein, both purified thermostable RuvB proteins showed strong double-s...

journal_title：Journal of bacteriology

authors： Tong J,Wetmur JG

更新日期：1996-05-01 00:00:00

abstract：:Expression of the Bacillus licheniformis alpha-amylase gene, amyL, was temporally activated and subject to catabolite repression both in its natural host and when cloned on a 3.55-kilobase fragment in Bacillus subtilis. A subclone from which the promoter region of amyL and sequences upstream from the promoter were del...

journal_title：Journal of bacteriology

authors： Laoide BM,Chambliss GH,McConnell DJ

更新日期：1989-05-01 00:00:00

abstract：:Glycerol was found to unravel the helical conformation of Escherichia coli type 1 fimbriae without appreciable depolymerization. The linearized fimbrial polymers have a diameter of 2 nm, react strongly with a monoclonal antibody directed at an inaccessible epitope on native fimbriae, and display greater mannose-bindin...

journal_title：Journal of bacteriology

authors： Abraham SN,Land M,Ponniah S,Endres R,Hasty DL,Babu JP

更新日期：1992-08-01 00:00:00

abstract：:Glucosylated deoxyribonucleic acid (DNA) from phages T4 and T6 competes poorly with homologous DNA causing only a slight decrease of transformation in Group H Streptococcus strain Challis. Other types of heterologous DNAs (Micrococcus luteus, Clostridium perfringens, Escherichia coli, calf thymus and non-glucosylated ...

journal_title：Journal of bacteriology

authors： Ceglowski P,Fuchs PG,Soltyk A

更新日期：1975-12-01 00:00:00

abstract：:The gene for the catabolic NAD-linked glutamate dehydrogenase of Peptostreptococcus asaccharolyticus was cloned by selection of Escherichia coli for complementation of a biosynthetic defect. Cloned fragments containing the gene and the P. asaccharolyticus transcription and translation signals are very highly expressed...

journal_title：Journal of bacteriology

authors： Snedecor B,Chu H,Chen E

更新日期：1991-10-01 00:00:00

abstract：:The glpD (MSMEG_6761) gene encoding glycerol-3-phosphate dehydrogenase was shown to be crucial for M. smegmatis to utilize glycerol as the sole carbon source. The glpD gene likely forms the glpFKD operon together with glpF and glpK, encoding a glycerol facilitator and glycerol kinase, respectively. The gylR (MSMEG_675...

journal_title：Journal of bacteriology

authors： Bong HJ,Ko EM,Song SY,Ko IJ,Oh JI

更新日期：2019-11-20 00:00:00

abstract：:Using PCR to create a probe based on conserved region 2 of sigma factors, we have cloned the sigA gene coding for the major sigma factor of Rhizobium meliloti. The 684-residue protein encoded by the sigA gene was expressed in vitro in coupled transcription-translation experiments with R. meliloti extracts and migrated...

journal_title：Journal of bacteriology

authors： Rushing BG,Long SR

更新日期：1995-12-01 00:00:00

abstract：:5-Azacytidine was found to be bactericidal to Escherichia coli carrying plasmids specifying EcoRII restriction-modification systems, but not to the same strains lacking these plasmids. Of other base analogs tested, only 5(beta-D-ribofuranosyl)isocytidine had similar, although weaker, effects. Plasmids that had lost th...

journal_title：Journal of bacteriology

authors： Friedman S

更新日期：1982-07-01 00:00:00

abstract：:Klebsiella pneumoniae is a nosocomial pathogen frequently isolated from opportunistic infections, especially in clinical environments. In spite of its potential pathogenicity, this microorganism has several metabolic potentials that could be used in biotechnology applications. K. pneumoniae is able to metabolize glyce...

journal_title：Journal of bacteriology

authors： Marçal D,Rêgo AT,Carrondo MA,Enguita FJ

更新日期：2009-02-01 00:00:00

abstract：:The rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (ELH I and ELH II) of Acinetobacter calcoaceticus were determined. The molecular weights of ELH I and ELH II were found by gel filtration to be approximately 24,000 and 21,000, respectively. In crude extract at...

journal_title：Journal of bacteriology

authors： Katagiri M,Wheelis ML

更新日期：1971-05-01 00:00:00

abstract：:A cell surface antigen complex from Zwittergent-solubilized Myxococcus xanthus has been purified by immunoaffinity chromatography with monoclonal antibody (MAb) 1604 and by subsequent gel filtration. We propose that the cell surface antigen (CSA) 1604 complex participates in intercellular interactions. The apparent to...

journal_title：Journal of bacteriology

authors： Jarvis BW,Dworkin M

更新日期：1989-09-01 00:00:00

abstract：:Glycosylation is a common modulation of protein function in eukaryotes and is biologically important. However, in bacteria protein glycosylation is rare, and relatively few bacterial glycoproteins are known. In Escherichia coli only two glycoproteins have been described to date. Here we introduce a novel member to thi...

journal_title：Journal of bacteriology

authors： Sherlock O,Dobrindt U,Jensen JB,Munk Vejborg R,Klemm P

更新日期：2006-03-01 00:00:00

abstract：:The segregation of mating loci with other unselected genes was analyzed in recombinants obtained from matings of Nocardia canicruria and N. erythropolis. The loci C/c and E/e control nocardial compatibility. Four mating genotype combinations were observed: cE, Ce, CE, and ce. Strains of N. erythropolis bear the genoty...

journal_title：Journal of bacteriology

authors： Brownell GH,Kelly KL

更新日期：1969-07-01 00:00:00

abstract：UNLABELLED:The GlnPQ transporter from Lactococcus lactis has the remarkable feature of having two substrate-binding domains (SBDs) fused to the N terminus of the transmembrane domain (TMD), and thus four SBDs are present in the homodimeric complex. Although X-ray structures and ligand binding data are available for bot...

journal_title：Journal of bacteriology

authors： Fulyani F,Schuurman-Wolters GK,Slotboom DJ,Poolman B

更新日期：2015-11-09 00:00:00

abstract：:Genetic and segregation analysis of Escherichia coli strains containing a partial duplication of the trp operon reveal that the 2.5-min-long region trpD-purB is duplicated in tandem in the chromosome. The adjacent loci cysB and fabD are not duplicated. Although one copy of the duplicated region is longer than the maxi...

journal_title：Journal of bacteriology

authors： Simonian MH,Goldstein RV,Mosteller RD

更新日期：1978-02-01 00:00:00

abstract：:The growth of strain araC(c)67, which produces the enzymes of the ara operon constitutively, is inhibited by the addition of ribitol. Isolation of strains resistant to ribitol yields mutants of either the araB or araC genes. A model to account for the inhibition by ribitol is discussed. ...

journal_title：Journal of bacteriology

authors： Katz L

更新日期：1970-05-01 00:00:00

abstract：:Starvation and cell density regulate the developmental expression of Myxococcus xanthus gene 4521. Three classes of mutants allow expression of this developmental gene during growth on nutrient agar, such that colonies of strains containing a Tn5 lac Omega4521 fusion are Lac(+). One class of these mutants inactivates ...

journal_title：Journal of bacteriology

authors： Guo D,Wu Y,Kaplan HB

更新日期：2000-08-01 00:00:00

abstract：:Salmonella typhimurium contains a transport system for sn-glycerol-3-phosphate that is inducible by growth on glycerol and sn-glycerol-3-phosphate. In fully induced cells, the system exhibited an apparent Km of 50 microM and a Vmax of 2.2 nmol/min . 10(8) cells. The corresponding system in Escherichia coli exhibits, u...

journal_title：Journal of bacteriology

authors： Hengge R,Larson TJ,Boos W

更新日期：1983-07-01 00:00:00

abstract：:The delta subunit of RNA polymerase, RpoE, is widespread in low-G+C Gram-positive bacteria and is thought to play a role in enhancing transcriptional specificity by blocking RNA polymerase binding at weak promoter sites and stimulating RNA synthesis by accelerating core enzyme recycling. Despite the well-studied bioch...

journal_title：Journal of bacteriology

authors： Xue X,Tomasch J,Sztajer H,Wagner-Döbler I

更新日期：2010-10-01 00:00:00

abstract：:Gene III (gIII) of phiLf, a filamentous phage specifically infecting Xanthomonas campestris pv. campestris, was previously shown to encode a virion-associated protein (pIII) required for phage adsorption. In this study, the transcription start site for the gene and the N-terminal sequence of the protein were determine...

journal_title：Journal of bacteriology

authors： Lin NT,Liu TJ,Lee TC,You BY,Yang MH,Wen FS,Tseng YH

更新日期：1999-04-01 00:00:00

abstract：:The addition of 1 mM orthophenanthroline (OP) into a mating mixture drastically reduced the production of recombinants. Examination of the effect of OP on each step of conjugation showed that the effect on the following steps could not account for the up to 500-fold reduction of recombinant formation: (i) preliminary ...

journal_title：Journal of bacteriology

authors： Ou JT,Reim R

更新日期：1976-10-01 00:00:00

abstract：:Replication-proficient (Rep+) revertants were isolated from mutants of IncFII plasmid NR1 that were replication defective (Rep-). The parental Rep- plasmids contained a mutation that inactivated promoter PE for transcription of RNA-E, a trans-acting repressor of translation of the essential RepA1 replication initiatio...

journal_title：Journal of bacteriology

authors： Wu R,Wang X,Womble DD,Rownd RH

更新日期：1993-05-01 00:00:00

abstract：:The effect of L-methionine-DL-sulfoximine, an inhibitor of glutamine synthetase, on the formation of nitrate reductase in the wild-type strain of Neurospora in the presence of ammonium ions and of glutamine was studied. Under conditions in which glutamine synthetase was inactivated, it was found that only glutamine co...

journal_title：Journal of bacteriology

authors： Premakumar R,Sorger GJ,Gooden D

更新日期：1980-07-01 00:00:00

abstract：:The reoxidation of NADH generated in reactions within the mitochondrial matrix of Saccharomyces cerevisiae is catalyzed by an NADH dehydrogenase designated Ndi1p (C. A. M. Marres, S. de Vries, and L. A. Grivell, Eur. J. Biochem. 195:857-862, 1991). Gene disruption analysis was used to examine possible metabolic functi...

journal_title：Journal of bacteriology

authors： Small WC,McAlister-Henn L

更新日期：1998-08-01 00:00:00