Bactericidal effect of 5-azacytidine on Escherichia coli carrying EcoRII restriction-modification enzymes.

abstract：:A temperate bacteriophage that determines the expression of enterohemolysin was isolated from Escherichia coli O26 strain C3888. The genetic determinant associated with enterohemolysin production (E-Hly determinant) was cloned from EcoRI-digested bacteriophage DNA in vector plasmid pUC8. pUC8 recombinant plasmid pEO19...

journal_title：Journal of bacteriology

authors： Beutin L,Bode L,Ozel M,Stephan R

更新日期：1990-11-01 00:00:00

abstract：:Reyranella massiliensis is an Alphaproteobacterium member of the class Rhodospirillaceae, growing in amoebae. We sequenced the genome of type strain 521(T). It is composed of a 5,792,218-bp chromosome and encodes 5,675 protein-coding genes and 53 RNA genes, including 3 rRNA genes. ...

journal_title：Journal of bacteriology

authors： Pagnier I,Croce O,Robert C,Raoult D,La Scola B

更新日期：2012-10-01 00:00:00

abstract：:Wood, N. P. (A. & M. College of Texas, College Station), and D. J. O'Kane. Formate-pyruvate exchange reaction in Streptococcus faecalis. I. Factor requirement for whole cells. J. Bacteriol. 87:97-103. 1964.-A factor present in plant and animal sources was found necessary for the incorporation of formate-C(14) into pyr...

journal_title：Journal of bacteriology

authors： WOOD NP,O'KANE DJ

更新日期：1964-01-01 00:00:00

abstract：:The Sinorhizobium meliloti megaplasmid pSymA has previously been implicated in gluconate utilization. We report a locus on pSymA encoding a putative tripartite ATP-independent periplasmic (TRAP) transporter that is required for gluconate utilization. The expression of this locus is negatively regulated by a GntR famil...

journal_title：Journal of bacteriology

authors： Steele TT,Fowler CW,Griffitts JS

更新日期：2009-02-01 00:00:00

abstract：:We show that both flanking IS256 elements carried by transposon Tn4001 are capable of generating head-to-tail tandem copies and free circular forms, implying that both are active. Our results suggest that the tandem structures arise from dimeric copies of the donor or vector plasmid present in the population by a mech...

journal_title：Journal of bacteriology

authors： Prudhomme M,Turlan C,Claverys JP,Chandler M

更新日期：2002-01-01 00:00:00

abstract：:This paper describes the major transmutations of donor deoxyribonucleic acid (DNA) after uptake by competent Bacillus subtilis cells. Kinetic experiments confirm that after exposure to competent cells, donor DNA is converted to double-stranded fragments (DSF) which can be isolated as early as 30 s from the beginning o...

journal_title：Journal of bacteriology

authors： Davidoff-Abelson R,Dubnau D

更新日期：1973-10-01 00:00:00

abstract：:A low level of recombination between homologous regions of plasmids and the host chromosome occurred during cell growth. The plasmids contained antibiotic resistance markers on the homologous regions which were only expressed when they were integrated into the chromosome. Such recombination took place in Rec+ rec-2 mu...

journal_title：Journal of bacteriology

authors： Balganesh M,Arrigoni L,Setlow JK

更新日期：1986-10-01 00:00:00

abstract：:Azotobacter vinelandii was grown diazotrophically in chemostat cultures limited by sucrose, citrate, or acetate. Specific activities of cellular oxygen consumption (qO2) and nitrogenase (acetylene reduction) were measured in situ at different dilution rates (D, representing the specific growth rate mu at steady state)...

journal_title：Journal of bacteriology

authors： Kuhla J,Oelze J

更新日期：1988-11-01 00:00:00

abstract：:We isolated 28 mutants of Bacillus subtilis deficient in the development of competence by using the transposon Tn917lacZ as a mutagen. The mutant strains were poorly transformable with plasmid and chromosomal DNAs but were normally transducible and exhibited wild-type resistance to DNA-damaging agents. The mutations w...

journal_title：Journal of bacteriology

authors： Hahn J,Albano M,Dubnau D

更新日期：1987-07-01 00:00:00

abstract：:The plasmid nature of genes specifying degradation of nicotine and nicotinate in Pseudomas convexa strain 1 (Pc1) is indicated by mitomycin curing and conjugational transfer to other strains. The NIC plasmid appears to be compatible with other metabolic plasmids in Pseudomonas putida. ...

journal_title：Journal of bacteriology

authors： Thacker R,Rørvig O,Kahlon P,Gunsalus IC

更新日期：1978-07-01 00:00:00

abstract：:A noncomposite transposon, designated Tn5541, was isolated from strain Fd33 of the filamentous cyanobacterium Fremyella diplosiphon UTEX 481. Sequence analysis showed that Tn5541 is structurally and genetically very similar to Tn5469, which is also endogenous to F. diplosiphon. Both Tn5469 and Tn5541 encode homologous...

journal_title：Journal of bacteriology

authors： Schaefer MR,Kahn K

更新日期：1998-11-01 00:00:00

abstract：:Pseudomonas oleovorans contains an isomerase which catalyzes the cis-trans conversion of the abundant unsaturated membrane fatty acids 9-cis-hexadecenoic acid (palmitoleic acid) and 11-cis-octadecenoic acid (vaccenic acid). We purified the isomerase from the periplasmic fraction of Pseudomonas oleovorans. The molecula...

journal_title：Journal of bacteriology

authors： Pedrotta V,Witholt B

更新日期：1999-05-01 00:00:00

abstract：:Division site placement in Escherichia coli involves interactions of the MinD protein with MinC and MinE and with other MinD molecules to form membrane-associated polymeric structures. In this work, as part of a study of these interactions, we established that heterologous membrane-associated proteins such as MinD can...

journal_title：Journal of bacteriology

authors： Taghbalout A,Ma L,Rothfield L

更新日期：2006-04-01 00:00:00

abstract：:In recent work, we described the excision of a large genomic region from Enterococcus faecium D344R in which the sequence from "joint" regions suggested that excision resulted from the interaction of conjugative transposon Tn916 and the related mobile element Tn5386. In the present study, we examined the ability of in...

journal_title：Journal of bacteriology

authors： Rice LB,Carias LL,Hutton-Thomas R,Rudin S

更新日期：2007-05-01 00:00:00

abstract：:Mycobacterium tuberculosis utilizes the methylerythritol phosphate (MEP) pathway for biosynthesis of isopentenyl diphosphate and its isomer, dimethylallyl diphosphate, precursors of all isoprenoid compounds. This pathway is of interest as a source of new drug targets, as it is absent from humans and disruption of the ...

journal_title：Journal of bacteriology

authors： Eoh H,Brown AC,Buetow L,Hunter WN,Parish T,Kaur D,Brennan PJ,Crick DC

更新日期：2007-12-01 00:00:00

abstract：:The neuS-encoded polysialytransferase (polyST) in Escherichia coli K1 catalyzes synthesis of polysialic acid homopolymers composed of unbranched sialyl alpha 2,8 linkages. Subcloning and complementation experiments showed that the K1 neuS was functionally interchangeable with the neuS from E. coli K92 (S. M. Steenberg...

journal_title：Journal of bacteriology

authors： Vimr ER,Bergstrom R,Steenbergen SM,Boulnois G,Roberts I

更新日期：1992-08-01 00:00:00

abstract：:Resistance of Staphylococcus aureus mediated by the penicillinase (Pc-ase) plasmid to divalent metal ions of Hg and Cd was found to be controlled by different mechanisms. The Hg resistance of the Pc-ase plasmid-carrying organisms is based upon a process of changing the ion incorporated in the cell into a somewhat inno...

journal_title：Journal of bacteriology

authors： Kondo I,Ishikawa T,Nakahara H

更新日期：1974-01-01 00:00:00

abstract：:During the progression of Mycoplasma hominis cultures from the early logarithmic phase to the stationary phase of growth, the total phospholipid content of the cell membranes decreased. Measurement of the amount of the various phospholipids during the growth cycle showed that a decrease in the phosphatidylglycerol (PG...

journal_title：Journal of bacteriology

authors： Rottem S,Greenberg AS

更新日期：1975-02-01 00:00:00

abstract：:Kinsky, Stephen C. (Washington University, St. Louis, Mo.). Induction and repression of nitrate reductase in Neurospora crassa. J. Bacteriol. 82:898-904. 1961.-Techniques are described for studying induced enzyme formation in Neurospora crassa. The effects of various parameters (time, pH, nitrate concentration, etc.) ...

journal_title：Journal of bacteriology

authors： KINSKY SC

更新日期：1961-12-01 00:00:00

abstract：:A DNA sequence (brpA) which regulates the expression of the genes of the bialaphos biosynthesis pathway (bap) in Streptomyces hygroscopicus was identified and characterized. A newly isolated nonproducing mutant (NP57) had a pleiotropic defect involving at least 6 of the 13 known bap genes; only the step 6 conversion c...

journal_title：Journal of bacteriology

authors： Anzai H,Murakami T,Imai S,Satoh A,Nagaoka K,Thompson CJ

更新日期：1987-08-01 00:00:00

abstract：:In Streptococcus mutans, competence for genetic transformation and biofilm formation are dependent on the two-component signal transduction system ComDE together with the inducer peptide pheromone competence-stimulating peptide (CSP) (encoded by comC). Here, it is shown that the same system is also required for expres...

journal_title：Journal of bacteriology

authors： van der Ploeg JR

更新日期：2005-06-01 00:00:00

abstract：:The abundance of oxidants and reductants must be balanced for an organism to thrive. Bacteria have evolved methods to prevent redox imbalances and to mitigate their deleterious consequences through the expression of detoxification enzymes, antioxidants, and systems to repair or degrade damaged proteins and DNA. Regula...

journal_title：Journal of bacteriology

authors： Reniere ML

更新日期：2018-08-10 00:00:00

abstract：:The minimum sequence of the enzymatic (A) subunit of Shiga toxin (STX) required for activity was investigated by introducing N-terminal and C-terminal deletions in the molecule. Enzymatic activity was assessed by using an in vitro translation system. A 253-amino-acid STX A polypeptide, which is recognized as the enzym...

journal_title：Journal of bacteriology

authors： Haddad JE,al-Jaufy AY,Jackson MP

更新日期：1993-08-01 00:00:00

abstract：:The gut microbiota plays a crucial role in the development of the immune system and confers benefits or disease susceptibility to the host. Emerging studies have indicated the gut microbiota could affect pulmonary health and disease through cross talk between the gut microbiota and the lungs. Gut microbiota dysbiosis c...

journal_title：Journal of bacteriology

authors： Zhou A,Lei Y,Tang L,Hu S,Yang M,Wu L,Yang S,Tang B

更新日期：2021-01-25 00:00:00

abstract：:Electron cryotomography was used to analyze the structure of the Lyme disease spirochete, Borrelia burgdorferi. This methodology offers a new means for studying the native architecture of bacteria by eliminating the chemical fixing, dehydration, and staining steps of conventional electron microscopy. Using electron cr...

journal_title：Journal of bacteriology

authors： Charon NW,Goldstein SF,Marko M,Hsieh C,Gebhardt LL,Motaleb MA,Wolgemuth CW,Limberger RJ,Rowe N

更新日期：2009-01-01 00:00:00

abstract：:The Escherichia coli structural gene for glutathione synthetase, gshB, was cloned into pBR322. Plasmids containing gshB were able to complement the glutathione requirement of a trxA gshB double mutant, and cells containing the plasmids were found to have elevated levels of glutathione synthetase. A mutant gshB allele ...

journal_title：Journal of bacteriology

authors： Daws T,Lim CJ,Fuchs JA

更新日期：1989-09-01 00:00:00

abstract：:Formylglutamate amidohydrolase (FGase) catalyzes the terminal reaction in the five-step pathway for histidine utilization in Pseudomonas putida. By this action, N-formyl-L-glutamate (FG) is hydrolyzed to produce L-glutamate plus formate. Urocanate, the first product in the pathway, induced all five enzymes, but FG was...

journal_title：Journal of bacteriology

authors： Hu L,Mulfinger LM,Phillips AT

更新日期：1987-10-01 00:00:00

abstract：:Oligopeptidase B (OpdB) is a serine peptidase broadly distributed among unicellular eukaryotes, gram-negative bacteria, and spirochetes which has emerged as an important virulence factor and potential therapeutic target in infectious diseases. We report here the cloning and expression of the opdB homologue from Salmon...

journal_title：Journal of bacteriology

authors： Morty RE,Fülöp V,Andrews NW

更新日期：2002-06-01 00:00:00

abstract：:Penicillium chrysogenum is an important producer of penicillin antibiotics. A key step in their biosynthesis is the oxidative cyclization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to isopenicillin N by the enzyme isopenicillin N synthase (IPNS). bis-ACV, the oxidized disulfide form of ACV is, however, ...

journal_title：Journal of bacteriology

authors： Cohen G,Argaman A,Schreiber R,Mislovati M,Aharonowitz Y

更新日期：1994-02-01 00:00:00

abstract：:When Bacillus subtilis is subjected to phosphate starvation, genes of the Pho regulon are either induced or repressed. Among those induced are genes encoding alkaline phosphatases (APases). A set of isogenic mutants, with a beta-galactosidase gene transcriptionally fused to the inactivated target gene, was used to ide...

journal_title：Journal of bacteriology

authors： Prágai Z,Eschevins C,Bron S,Harwood CR

更新日期：2001-04-01 00:00:00