Glycerol-induced unraveling of the tight helical conformation of Escherichia coli type 1 fimbriae.

abstract：:The Escherichia coli dnaN159 allele encodes a mutant form of the beta-sliding clamp (beta159) that is impaired for interaction with the replicative DNA polymerase (Pol), Pol III. In addition, strains bearing the dnaN159 allele require functional Pol I for viability. We have utilized a combination of genetic and bioche...

journal_title：Journal of bacteriology

authors： Maul RW,Sanders LH,Lim JB,Benitez R,Sutton MD

更新日期：2007-07-01 00:00:00

abstract：:The glycan chains in peptidoglycan or murein are cross-linked by transpeptidation of the peptide side chains. To assess the fraction of side chains involved in cross-bridges, distinction has been made between cross-linkage and cross-linking. The first expression refers to the situation in unlabeled (or fully labeled) ...

journal_title：Journal of bacteriology

authors： Driehuis F,de Jonge B,Nanninga N

更新日期：1992-03-01 00:00:00

abstract：:ULTRACENTRIFUGATION IN SUCROSE DENSITY GRADIENTS WAS EMPLOYED TO ESTIMATE THE MOLECULAR WEIGHTS AND TO DETERMINE POSSIBLE PHYSICAL AGGREGATION OF THE FIVE ENZYMES CATALYZING STEPS TWO TO SIX IN THE PRECHORISMIC ACID PORTION OF THE POLYAROMATIC SYNTHETIC PATHWAY IN SIX SPECIES OF BACTERIA: Escherichia coli, Salmonella ...

journal_title：Journal of bacteriology

authors： Berlyn MB,Giles NH

更新日期：1969-07-01 00:00:00

abstract：:Binding of Saccharomyces cerevisiae alpha-agglutinin to target a cells was assayed by agglutination inhibition and 125I-alpha-agglutinin binding. The assays showed characteristics of equilibrium binding, namely saturability, competability, and the establishment of a kinetic endpoint in the presence of free alpha-agglu...

journal_title：Journal of bacteriology

authors： Lipke PN,Terrance K,Wu YS

更新日期：1987-02-01 00:00:00

abstract：:An open-reading-frame fragment of a Methylobacterium sp. strain AM1 gene (moxF) encoding a portion of the methanol dehydrogenase structural protein has been used as a hybridization probe to detect similar sequences in a variety of methylotrophic bacteria. This hybridization was used to isolate clones containing putati...

journal_title：Journal of bacteriology

authors： Stephens RL,Haygood MG,Lidstrom ME

更新日期：1988-05-01 00:00:00

abstract：:Hook-associated proteins (HAPs) were excreted into the culture medium of the Fla+ strain as well as into the growth medium of the filamentless mutants of Salmonella typhimurium. This indicates that the bacteria synthesize HAPs excessively, beyond the amount required for construction of flagella. The extra HAPs are she...

journal_title：Journal of bacteriology

authors： Homma M,Iino T

更新日期：1985-12-01 00:00:00

abstract：:Of 16 restriction endonucleases known to hydrolyze rare 6- or 8-base recognition sequences that were tested, only SpeI, NotI, AscI, and SfiI generated fragments of chromosomal DNA from Yersinia pestis, the causative agent of bubonic plague, of sufficient length to permit physical analysis by pulsed-field gel electroph...

journal_title：Journal of bacteriology

authors： Lucier TS,Brubaker RR

更新日期：1992-04-01 00:00:00

abstract：:Faithful DNA replication involves the removal of RNA residues from genomic DNA prior to the ligation of nascent DNA fragments in all living organisms. Because the physiological roles of archaeal type 2 RNase H are not fully understood, the substrate structure requirements for the detection of RNase H activity need fur...

journal_title：Journal of bacteriology

authors： Le Laz S,Le Goaziou A,Henneke G

更新日期：2010-07-01 00:00:00

abstract：:An in vitro transposition system, developed to facilitate gene disruption in Deinococcus radiodurans R1, has been used to inactivate the gene designated dr1819 in uvrA-1(+) and uvrA-1 backgrounds. dr1819 encodes a protein with homology to a UV DNA damage endonuclease expressed by Schizosaccharomyces pombe. Interruptio...

journal_title：Journal of bacteriology

authors： Earl AM,Rankin SK,Kim KP,Lamendola ON,Battista JR

更新日期：2002-02-01 00:00:00

abstract：:The EutD protein of Salmonella enterica is homologous to the catalytic domain of the phosphotransacetylase (Pta) enzyme. The Pta-like activity level of the EutD enzyme compared favorably to that of other Pta enzymes. High-pressure liquid chromatography and mass spectrometry verified that acetyl-coenzyme A was the prod...

journal_title：Journal of bacteriology

authors： Brinsmade SR,Escalante-Semerena JC

更新日期：2004-03-01 00:00:00

abstract：:The potassium, calcium, magnesium, and zinc ion content of cells in exponential and synchronously growing cultures of Escherichia coli B/r was determined with an X-ray fluorescence spectrometer and an atomic absorption spectrophotometer. Cellular potassium, calcium, and magnesium content increased smoothly during the ...

journal_title：Journal of bacteriology

authors： Kung FC,Raymond J,Glaser DA

更新日期：1976-06-01 00:00:00

abstract：:Stocks, Peter K. (Louisiana State University, Baton Rouge), and C. S. McCleskey. Identity of the pink-pigmented methanol-oxidizing bacteria as Vibrio extorquens. J. Bacteriol. 88:1065-1070. 1964.-Pink-pigmented bacteria isolated from enrichment cultures of methane oxidizers were found to possess similar morphological,...

journal_title：Journal of bacteriology

authors： STOCKS PK,MCCLESKEY CS

更新日期：1964-10-01 00:00:00

abstract：:Two chemotactic transducers for inorganic phosphate (P(i)), designated CtpH and CtpL, have been identified in Pseudomonas aeruginosa. The corresponding genes (ctpH and ctpL) were inactivated by inserting kanamycin and tetracycline resistance gene cassettes into the wild-type genes in the P. aeruginosa PAO1 genome. Com...

journal_title：Journal of bacteriology

authors： Wu H,Kato J,Kuroda A,Ikeda T,Takiguchi N,Ohtake H

更新日期：2000-06-01 00:00:00

abstract：:Beta toxin is a neutral sphingomyelinase secreted by certain strains of Staphylococcus aureus. This virulence factor lyses erythrocytes in order to evade the host immune system as well as scavenge nutrients. The structure of beta toxin was determined at 2.4-A resolution using crystals that were merohedrally twinned. T...

journal_title：Journal of bacteriology

authors： Huseby M,Shi K,Brown CK,Digre J,Mengistu F,Seo KS,Bohach GA,Schlievert PM,Ohlendorf DH,Earhart CA

更新日期：2007-12-01 00:00:00

abstract：:The tdh promoter of Escherichia coli is induced seven- to eightfold when cells are grown in the presence of exogenous leucine. A scheme was devised to select mutants that exhibited high constitutive expression of the tdh promoter. The mutations in these strains were shown to lie within a previously identified gene (lr...

journal_title：Journal of bacteriology

authors： Rex JH,Aronson BD,Somerville RL

更新日期：1991-10-01 00:00:00

abstract：:Poly(U)- and poly(UG)-programmed cell-free systems were developed from the extreme thermophilic, anaerobic eubacterium Thermotoga maritima, and their susceptibility to aminoglycoside and other antibiotics was assayed at a temperature (75 degrees C) close to the physiological optimum (80 degrees C) for cell growth and ...

journal_title：Journal of bacteriology

authors： Londei P,Altamura S,Huber R,Stetter KO,Cammarano P

更新日期：1988-09-01 00:00:00

abstract：:L-Glutamate decarboxylase (GAD) activity of Clostridium perfringens (ATCC 8009) cells grown in various culture conditions was investigated. Remarkable variations of GAD level occur during the growth cycle in thioglycollate broth. These changes are affected by the pH of the culture medium. Addition of alkali to the cul...

journal_title：Journal of bacteriology

authors： Cozzani I,Barsacchi R,Dibenedetto G,Saracchi L,Falcone G

更新日期：1975-09-01 00:00:00

abstract：:Numerous bacteria and mammalian cells harbor two enzymes, phosphopentomutase (PPM) and 2-deoxyribose 5-phosphate aldolase (DERA), involved in the interconversion between nucleosides and central carbon metabolism. In this study, we have examined the presence of this metabolic link in the hyperthermophilic archaeon, The...

journal_title：Journal of bacteriology

authors： Rashid N,Imanaka H,Fukui T,Atomi H,Imanaka T

更新日期：2004-07-01 00:00:00

abstract：:ATP sulfurylase is a key enzyme in the energy-generating sulfur oxidation pathways of many chemoautotrophic bacteria. The utilization of reduced sulfur compounds to fuel CO2 fixation by the still-uncultured bacterial endosymbionts provides the basis of nutrition in invertebrates, such as the tubeworm Riftia pachyptila...

journal_title：Journal of bacteriology

authors： Laue BE,Nelson DC

更新日期：1994-06-01 00:00:00

abstract：:In the microbial conversion of added hypoxanthine to 5'-inosinic acid, Mn(2+) concentration in the growth medium is known to have a profound effect both on the yield of 5'-inosinic acid and the morphology of cells of Brevibacterium ammoniagenes. To elucidate the mechanism in which Mn(2+) was concerned with cell morpho...

journal_title：Journal of bacteriology

authors： Oka T,Udagawa K,Kinoshita S

更新日期：1968-11-01 00:00:00

abstract：:The spx gene encodes an RNA polymerase-binding protein that exerts negative and positive transcriptional control in response to oxidative stress in Bacillus subtilis. It resides in the yjbC-spx operon and is transcribed from at least five promoters located in the yjbC regulatory region or in the yjbC-spx intergenic re...

journal_title：Journal of bacteriology

authors： Leelakriangsak M,Kobayashi K,Zuber P

更新日期：2007-03-01 00:00:00

abstract：:Agrobacterium tumefaciens R10 was mutagenized by using the promoter probe transposon Tn5-gusA7, and a library of approximately 5,000 transcriptional fusions was screened for octopine-inducible patterns of gene expression. Twenty-one mutants carrying strongly inducible gusA fusions, 20 of which showed defects in the ca...

journal_title：Journal of bacteriology

authors： Cho K,Fuqua C,Martin BS,Winans SC

更新日期：1996-04-01 00:00:00

abstract：:The lic3 locus of Haemophilus influenzae consists of four open reading frames. The derived amino acid sequences of orf2 and orf4 exhibit homology to Escherichia coli GalE and AdK, respectively. The functions of orf1 and orf3 remain unknown. orf1 contains multiple tandem repeats of the tetrameric DNA sequence CAAT near...

journal_title：Journal of bacteriology

authors： Szabo M,Maskell D,Butler P,Love J,Moxon R

更新日期：1992-11-01 00:00:00

abstract：:Many bacteria can accumulate glycine betaine for osmoprotection and catabolize it as a growth substrate, but how they regulate these opposing roles is poorly understood. In Pseudomonas syringae B728a, expression of the betaine catabolism genes was reduced by an osmotic upshift to an intermediate stress level, consiste...

journal_title：Journal of bacteriology

authors： Li S,Yu X,Beattie GA

更新日期：2013-05-01 00:00:00

abstract：:Transition metals are essential for life but are toxic when in excess. Metal ion intoxication may result from the mismetallation of essential metal-dependent enzymes with a noncognate metal. To begin to identify enzymes and processes that are susceptible to mismetallation, we have selected for strains with increased r...

journal_title：Journal of bacteriology

authors： Pi H,Wendel BM,Helmann JD

更新日期：2020-03-11 00:00:00

abstract：:The ability to restart broken DNA replication forks is essential across all domains of life. In Escherichia coli, the priA, priB, priC, and dnaT genes encode the replication restart proteins (RRPs) to accomplish this task. PriA plays a critical role in replication restart such that its absence reveals a dramatic pheno...

journal_title：Journal of bacteriology

authors： Leroux M,Jani N,Sandler SJ

更新日期：2017-08-08 00:00:00

abstract：:Heme A, as a prosthetic group, is found exclusively in respiratory oxidases of mitochondria and aerobic bacteria. Bacillus subtilis CtaA and other heme A synthases catalyze the conversion of a methyl side group on heme O into a formyl group. The catalytic mechanism of heme A synthase is not understood, and little is k...

journal_title：Journal of bacteriology

authors： Hederstedt L,Lewin A,Throne-Holst M

更新日期：2005-12-01 00:00:00

abstract：:The opportunistic pathogen Pseudomonas aeruginosa produces multifunctional, polar, filamentous appendages termed type IV pili. Type IV pili are involved in colonization during infection, twitching motility, biofilm formation, bacteriophage infection, and natural transformation. Electrostatic surface analysis of modele...

journal_title：Journal of bacteriology

authors： van Schaik EJ,Giltner CL,Audette GF,Keizer DW,Bautista DL,Slupsky CM,Sykes BD,Irvin RT

更新日期：2005-02-01 00:00:00

abstract：:When an Escherichia coli K-12 culture was starved for glucose, 50% of the cells lost viability in about 6 days. When a K-12 mutant lacking five distinct peptidase activities, CM89, was starved in the same manner, viability was lost much more rapidly; 50% of the cells lost viability in about 2 days, whereas a parent st...

journal_title：Journal of bacteriology

authors： Reeve CA,Bockman AT,Matin A

更新日期：1984-03-01 00:00:00

abstract：:Many of the gene products that participate in nitrogen metabolism are sensitive to nitrogen catabolite repression (NCR), i.e., their expression is decreased to low levels when readily used nitrogen sources such as asparagine are provided. Previous work has shown this NCR sensitivity requires the cis-acting UASNTR elem...

journal_title：Journal of bacteriology

authors： Coffman JA,el Berry HM,Cooper TG

更新日期：1994-12-01 00:00:00