Abstract:
:The spx gene encodes an RNA polymerase-binding protein that exerts negative and positive transcriptional control in response to oxidative stress in Bacillus subtilis. It resides in the yjbC-spx operon and is transcribed from at least five promoters located in the yjbC regulatory region or in the yjbC-spx intergenic region. Induction of spx transcription in response to treatment with the thiol-specific oxidant diamide is the result of transcription initiation at the P(3) promoter located upstream of the spx coding sequence. Previous studies conducted elsewhere and analyses of transcription factor mutants using transformation array technology have uncovered two transcriptional repressors, PerR and YodB, that target the cis-acting negative control elements of the P(3) promoter. Expression of an spx-bgaB fusion carrying the P(3) promoter is elevated in a yodB or perR mutant, and an additive increase in expression was observed in a yodB perR double mutant. Primer extension analysis of spx RNA shows the same additive increase in P(3) transcript levels in yodB perR mutant cells. Purified YodB and PerR repress spx transcription in vitro when wild-type spx P(3) promoter DNA was used as a template. Point mutations at positions within the P(3) promoter relieved YodB-dependent repression, while a point mutation at position +24 reduced PerR repression. DNase I footprinting analysis showed that YodB protects a region that includes the P(3) -10 and -35 regions, while PerR binds to a region downstream of the P(3) transcriptional start site. The binding of both repressors is impaired by the treatment of footprinting reactions with diamide or hydrogen peroxide. The study has uncovered a mechanism of dual negative control that relates to the oxidative stress response of gram-positive bacteria.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Leelakriangsak M,Kobayashi K,Zuber Pdoi
10.1128/JB.01520-06subject
Has Abstractpub_date
2007-03-01 00:00:00pages
1736-44issue
5eissn
0021-9193issn
1098-5530pii
JB.01520-06journal_volume
189pub_type
杂志文章abstract::A deoxyribonucleic acid-directed in vitro system for the synthesis of Escherichia coli carbamoylphosphate synthase has been developed, and its properties have been studied. The system uses the deoxyribonucleic acid of a lambda phage carrying the car genes (lambdadcarAB) as template and mediates the synthesis of both s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.1.58-66.1980
更新日期:1980-01-01 00:00:00
abstract::Several enzymes and metabolites concerned with carbohydrate metabolism were examined in mice infected with Listeria monocytogenes. Liver glycogen and glucose decreased parallel to severity of infection. The concentration of glucose in the blood fell to abnormally low levels with a hypoglycemia being most evident at 72...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.2.538-543.1967
更新日期:1967-02-01 00:00:00
abstract::Pyrrolnitrin at 10 mug/ml inhibited the growth of Saccharomyces cerevisiae, Penicillium atrovenetum, and P. oxalicum. The primary site of action of pyrrolnitrin on S. cerevisiae was the terminal electron transport system between succinate or reduced nicotinamide adenine dinucleotide (NADH) and coenzyme Q. At growth in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.310-318.1969
更新日期:1969-10-01 00:00:00
abstract::Ralstonia eutropha JMP134(pJP4) degrades 3-chlorobenzoate (3-CB) by using two not completely isofunctional, pJP4-encoded chlorocatechol degradation gene clusters, tfdC(I)D(I)E(I)F(I) and tfdD(II)C(II)E(II)F(II). Introduction of several copies of each gene cluster into R. eutropha JMP222, which lacks pJP4 and thus accu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.5.1534-1542.2003
更新日期:2003-03-01 00:00:00
abstract::Burkholderia species, including opportunistic pathogens in the Burkholderia cepacia complex (Bcc), have genes to produce contact-dependent growth inhibition (CDI) system proteins. CDI is a phenomenon in which Gram-negative bacteria use the toxic C terminus of a polymorphic surface-exposed exoprotein, BcpA, to inhibit ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00012-19
更新日期:2019-05-22 00:00:00
abstract::Birnbaum, Jerome (University of Cincinnati, Cincinnati, Ohio), and Herman C. Lichstein. Metabolism of biotin and analogues of biotin by microorganisms. IV. Degradation of biotin, oxybiotin, and desthiobiotin by Lactobacillus casei. J. Bacteriol. 92:925-930. 1966.-Lactobacillus casei degrades biotin when it is present ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.4.925-930.1966
更新日期:1966-10-01 00:00:00
abstract::Using an oligonucleotide microarray, we searched for previously unrecognized transcription units in intergenic regions in the genome of Bacillus subtilis, with an emphasis on identifying small genes activated during spore formation. Nineteen transcription units were identified, 11 of which were shown to depend on one ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00534-10
更新日期:2010-10-01 00:00:00
abstract::Evidence for the existence of two molecular species of exfoliative toxin (ET) synthesized by Staphylococcus hyicus (SHET) under chromosomal and plasmid control is presented. Serological evidence that these molecular species of toxins are distinct from each other is given. The molecular weights of SHET from plasmidless...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.14.4096-4100.2000
更新日期:2000-07-01 00:00:00
abstract::The gamma-proteobacterium Francisella tularensis is one of the most infectious human pathogens, and the highly virulent organism F. tularensis subsp. tularensis (type A) and less virulent organism F. tularensis subsp. holarctica (type B) are most commonly associated with significant disease in humans and animals. Here...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00506-06
更新日期:2006-10-01 00:00:00
abstract::Grixazone (GX), which is a diffusible yellow pigment containing a phenoxazinone chromophore, is one of the secondary metabolites under the control of A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) in Streptomyces griseus. GX production is also induced by phosphate starvation. The whole biosynthesis gen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00055-07
更新日期:2007-05-01 00:00:00
abstract::The ski22::Tn917lac insertion mutation in Bacillus subtilis was isolated in a screen for mutations that cause a defect in sporulation but are suppressed by the presence or overexpression of the histidine protein kinase encoded by kinA (spoIIJ). The ski22::Tn917lac insertion mutation was in ald, the gene encoding alani...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.21.6789-6796.1993
更新日期:1993-11-01 00:00:00
abstract::A Mycoplasma gallisepticum subcellular fraction (P2), which contains the deoxyribonucleic acid replication complex, can be isolated by differential centrifugation of freeze-thaw-lysed cells. The nascent deoxyribonucleic acid is released from P2 by Lubrol-WX, sodium dodecyl sulfate, Pronase, and deoxyribonuclease, but ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.495-501.1974
更新日期:1974-10-01 00:00:00
abstract::The reversion behavior of pleiotropic carbohydrate mutants, previously designated as ctr, was studied. The mutants revert to complete restoration of the wild-type phenotype, as well as to a spectrum of partial wild-type phenotypes. Lac(+) reversions were found in the lac region (11 min) and some Mal(+) reversions occu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.3.1318-1324.1970
更新日期:1970-12-01 00:00:00
abstract::The genes encoding the two isoenzymes of methyl coenzyme M reductase (MRI and MRII) in Methanobacterium thermoautotrophicum delta H have been cloned and sequenced. The MRI-encoding mcr operon (mcrBDCGA) has been located immediately upstream from the mtr operon (mtrEDCBA) that encodes N5-methyltetrahydromethanopterin:c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.20.6384-6391.1994
更新日期:1994-10-01 00:00:00
abstract::Methanococcus aeolicus, Methanococcus maripaludis, and Methanococcus voltae contain similar levels of four enzymes of branched-chain amino acid biosynthesis: acetohydroxy acid synthase, acetohydroxy acid isomeroreductase, dihydroxy acid dehydratase, and transaminase B. Following growth at low partial pressures of H2-C...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.6.2086-2092.1991
更新日期:1991-03-01 00:00:00
abstract::A key reaction in the biosynthesis of menaquinone involves the conversion of the soluble bicyclic naphthalenoid compound 1, 4-dihydroxy-2-naphthoic acid (DHNA) to the membrane-bound demethylmenaquinone. The enzyme catalyzing this reaction, DHNA-octaprenyltransferase, attaches a 40-carbon side chain to DHNA. The menA g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.10.2782-2787.1998
更新日期:1998-05-01 00:00:00
abstract::Two open reading frames (ORFs) were identified immediately downstream of the four structural genes for the soluble hydrogenase (SH) of Alcaligenes eutrophus H16. While a mutation in ORF2 had no obvious effect on hydrogen metabolism, an in-frame deletion in ORF1, subsequently designated hoxW, led to a complete loss of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.8.2368-2374.1996
更新日期:1996-04-01 00:00:00
abstract::A macrorestriction map representing the complete physical map of the Rhodobacter sphaeroides 2.4.1 chromosomes has been constructed by ordering the chromosomal DNA fragments from total genomic DNA digested with the restriction endonucleases AseI, SpeI, DraI, and SnaBI. Junction fragments and multiple restriction endon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.5850-5859.1989
更新日期:1989-11-01 00:00:00
abstract::Bacteriophage phi29 protein p6 is a viral architectural protein, which binds along the whole linear phi29 DNA in vivo and is involved in initiation of DNA replication and transcription control. Protein p1 is a membrane-associated viral protein, proposed to attach the viral genome to the cell membrane. Protein p17 is i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.24.8401-8406.2004
更新日期:2004-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.8.2466-2475.2004
更新日期:2004-04-01 00:00:00
abstract::Production of Bacillus subtilis exoproteases is positively regulated by the DegS-DegU two-component regulatory system and other regulatory factors including DegR and ProB. It was shown that the expression of degR was virtually abolished in a sigD mutant and that the transcriptional initiation site in vivo is preceded ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.1.216-222.1996
更新日期:1996-01-01 00:00:00
abstract::The ubiquitous opportunistic pathogen Pseudomonas aeruginosa has five aerobic terminal oxidases: bo(3)-type quinol oxidase (Cyo), cyanide-insensitive oxidase (CIO), aa3-type cytochrome c oxidase (aa3), and two cbb(3)-type cytochrome c oxidases (cbb(3)-1and cbb(3)-2). These terminal oxidases are differentially regulate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02176-14
更新日期:2014-12-01 00:00:00
abstract::Obligate intracellular bacteria comprising the order Chlamydiales lack the ability to synthesize nucleotides de novo and must acquire these essential compounds from the cytosol of the host cell. The environmental protozoan endosymbiont Protochlamydia amoebophila UWE25 encodes five nucleotide transporters with specific...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00433-13
更新日期:2013-08-01 00:00:00
abstract::The exit of glutamate from Escherichia coli K-12 cells preloaded with the radioactive amino acid and its relation to the reaction of entry were studied. Experiments with cells preloaded to different intracellular concentrations of radioactive glutamate confirmed our earlier conclusion that glutamate exit was a first-o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.1.51-57.1973
更新日期:1973-01-01 00:00:00
abstract::Germaine, G. R. (University of Minnesota, Minneapolis), and D. L. Anderson. Binding of homologous polymerized deoxyribonucleic acid by Streptomyces griseus. J. Bacteriol. 92:662-667. 1966-An irreversible P(32) deoxyribonucleic acid (DNA) binding system is described for Streptomyces griseus S104. The 69% guanine plus c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.3.662-667.1966
更新日期:1966-09-01 00:00:00
abstract::Escherichia coli acetyl coenzyme A carboxylase (ACC), the first enzyme of the fatty acid biosynthetic pathway, is inhibited by acylated derivatives of acyl carrier protein (ACP). ACP lacking an acyl moiety does not inhibit ACC. Acylated derivatives of ACP having chain lengths of 6 to 20 carbon atoms were similarly inh...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.4.1499-1503.2001
更新日期:2001-02-01 00:00:00
abstract::We used cells carrying plasmids causing the overproduction of Gln3p, Ure2p, or both of these proteins to elucidate the ability of Ure2p to prevent the activation of gene expression by Gln3p in cells growing in a glutamine-containing medium. Our results indicate that Ure2p probably does not interfere with the binding o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.15.4734-4736.1996
更新日期:1996-08-01 00:00:00
abstract::Brucella native haptens (NHs) extracted with hot water from smooth (S)-type B. abortus and B. melitensis were purified to high levels of serological activity and compared with the polysaccharide obtained by acid hydrolysis (PS) of the S lipopolysaccharide (S-LPS). By 13C nuclear magnetic resonance analysis, NHs showed...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.4.1070-1079.1996
更新日期:1996-02-01 00:00:00
abstract::Rhodobacter sphaeroides cells containing an in-frame deletion within ccmA lack detectable soluble and membrane-bound c-type cytochromes and are unable to grow under conditions where these proteins are required. Only strains merodiploid for ccmABCDG were found after attempting to generate cells containing either a ccmG...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.15.4643-4647.2001
更新日期:2001-08-01 00:00:00
abstract::Metal-dependent superoxide dismutases (SODs) with a specific requirement for a manganese or iron ion for catalytic activity and copper- and zinc-dependent enzymes are essential for detoxification of superoxide anion radicals. Genome sequence analyses predict the existence of a nickel-dependent enzyme (NiSOD) as the un...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.22.7821-7825.2004
更新日期:2004-11-01 00:00:00