Abstract:
:The gamma-proteobacterium Francisella tularensis is one of the most infectious human pathogens, and the highly virulent organism F. tularensis subsp. tularensis (type A) and less virulent organism F. tularensis subsp. holarctica (type B) are most commonly associated with significant disease in humans and animals. Here we report the complete genome sequence and annotation for a low-passage type B strain (OSU18) isolated from a dead beaver found near Red Rock, Okla., in 1978. A comparison of the F. tularensis subsp. holarctica sequence with that of F. tularensis subsp. tularensis strain Schu4 (P. Larsson et al., Nat. Genet. 37:153-159, 2005) highlighted genetic differences that may underlie different pathogenicity phenotypes and the evolutionary relationship between type A and type B strains. Despite extensive DNA sequence identity, the most significant difference between type A and type B isolates is the striking amount of genomic rearrangement that exists between the strains. All but two rearrangements can be attributed to homologous recombination occurring between two prominent insertion elements, ISFtu1 and ISFtu2. Numerous pseudogenes have been found in the genomes and are likely contributors to the difference in virulence between the strains. In contrast, no rearrangements have been observed between the OSU18 genome and the genome of the type B live vaccine strain (LVS), and only 448 polymorphisms have been found within non-transposase-coding sequences whose homologs are intact in OSU18. Nonconservative differences between the two strains likely include the LVS attenuating mutation(s).
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Petrosino JF,Xiang Q,Karpathy SE,Jiang H,Yerrapragada S,Liu Y,Gioia J,Hemphill L,Gonzalez A,Raghavan TM,Uzman A,Fox GE,Highlander S,Reichard M,Morton RJ,Clinkenbeard KD,Weinstock GMdoi
10.1128/JB.00506-06subject
Has Abstractpub_date
2006-10-01 00:00:00pages
6977-85issue
19eissn
0021-9193issn
1098-5530pii
188/19/6977journal_volume
188pub_type
杂志文章abstract::Ethanolamine (EA) metabolism is a trait associated with enteric pathogens, including enterohemorrhagic Escherichia coli O157:H7 (EHEC). EHEC causes severe bloody diarrhea and hemolytic uremic syndrome. EHEC encodes the ethanolamine utilization (eut) operon that allows EHEC to metabolize EA and gain a competitive advan...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00937-13
更新日期:2013-11-01 00:00:00
abstract::Burchard, Robert P. (University of Minnesota, Minneapolis), and Martin Dworkin. Light-induced lysis and carotenogenesis in Myxococcus xanthus. J. Bacteriol. 91:535-545. 1966.-Myxococcus xanthus, grown vegetatively in the light, developed an orange carotenoid after the cells entered stationary phase of growth; pigment ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.2.535-545.1966
更新日期:1966-02-01 00:00:00
abstract::Staphylococcus aureus responds with protective strategies against phagocyte-derived reactive oxidants to infect humans. Herein, we report the transcriptome analysis of the cellular response of S. aureus to hydrogen peroxide-induced oxidative stress. The data indicate that the oxidative response includes the induction ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.4.1648-1659.2006
更新日期:2006-02-01 00:00:00
abstract::Gene conversion, defined as the nonreciprocal transfer of DNA, is one result of homologous recombination. Three steps in recombination could give rise to gene conversion: (i) DNA synthesis for repair of the degraded segment, (ii) Holliday junction migration, leading to heteroduplex formation, and (iii) repair of misma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00111-09
更新日期:2009-08-01 00:00:00
abstract::Cyclic nucleotide-independent protein kinase (EC 2.7.1.37) activity was found in the nuclear cap organelle, within which ribosomes of zoospores of Blastocladiella emersonii are sequestered. Two protein kinase activities were resolved from the high-salt wash fraction of zoospore ribosomes by selective adsorption to DEA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.3.1467-1471.1983
更新日期:1983-06-01 00:00:00
abstract::Environmental iron concentrations coordinately regulate transcription of genes involved in iron acquisition and virulence via the ferric uptake regulation (fur) system. We identified and sequenced the fur gene and flanking regions of three Bartonella species. The most notable difference between Bartonella Fur and othe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.19.5751-5755.2001
更新日期:2001-10-01 00:00:00
abstract::Chronic lung infections in cystic fibrosis (CF) could be treated more effectively if the effects of antimicrobials on pathogens in situ were known. Here, we compared changes in the microbial community composition and pathogen growth rates in longitudinal studies of seven pediatric CF patients undergoing intravenous an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00365-18
更新日期:2018-11-26 00:00:00
abstract::Escherichia coli sigma70-dependent promoters have typically been characterized as either -10/-35 promoters, which have good matches to both the canonical -10 and the -35 sequences or as extended -10 promoters (TGn/-10 promoters), which have the TGn motif and an excellent match to the -10 consensus sequence. We report ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00853-06
更新日期:2006-12-01 00:00:00
abstract::The isocitrate lyase from a thermophilic Bacillus is activated about threefold by a variety of salts. Such strong stimulation of activity is not seen with isocitrate lyase from the mesophiles, Bacillus licheniformis, Bacillus megaterium, Escherichia coli, and Aspergillus nidulans. The salt activation is markedly pH-de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.3.1160-1169.1973
更新日期:1973-12-01 00:00:00
abstract::In Salmonella, ilv-linked rfe genes participate in the biosynthesis of the enterobacterial common antigen (CA) as well as of certain types of O antigen (serogroups C1 and L). rff genes, probably in the same cluster with rfe, are required for CA synthesis (P.H. Mäkelä et al., in preparation). Several Escherichia coli s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.2.755-762.1976
更新日期:1976-08-01 00:00:00
abstract::Methanol:5-hydroxybenzimidazolylcobamide methyltransferase (MT1) is the first of two enzymes required for transfer of the methyl group of methanol to 2-mercaptoethanesulfonic acid in Methanosarcina barkeri. MT1 binds the methyl group of methanol to its corrinoid prosthetic group only when the central cobalt atom of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.5.1278-1283.1993
更新日期:1993-03-01 00:00:00
abstract::Upon induction, Bacillus megaterium 216 produces the bacteriocin megacin A-216, which leads to lysis of the producer cell and kills B. megaterium and a few other bacterial species. The DNA region responsible for megacinogeny was cloned in B. megaterium. The nucleotide sequence of a 5,494-bp-long subfragment was determ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00557-08
更新日期:2008-10-01 00:00:00
abstract::The pH dependence of transport of the neutral amino acids L-serine and L-alanine by membrane vesicles of Streptococcus cremoris have been studied in detail. The rates of four modes of facilitated diffusion (e.g., influx, efflux, exchange, and counterflow) of L-serine and L-alanine increase with increasing H+ concentra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2748-2754.1987
更新日期:1987-06-01 00:00:00
abstract::The cloned gene coding for Bacillus licheniformis penicillinase (penP) was introduced into Escherichia coli in a heat-inducible lambda Qam vector. After induction, significant amounts of penicillinase were synthesized in the new host. The cellular location of the penicillinase was found to be almost exclusively the ou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.2.657-663.1983
更新日期:1983-08-01 00:00:00
abstract::The incA gene product of ColE2-P9 and ColE3-CA38 plasmids is an antisense RNA that regulates the production of the plasmid-coded Rep protein essential for replication. The Rep protein specifically binds to the origin and synthesizes a unique primer RNA at the origin. The IncB incompatibility is due to competition for ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.23.7233-7243.1994
更新日期:1994-12-01 00:00:00
abstract::A set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described along with methods for their use and examples. One pair of primers is capable of amplifying nearly full-length 16S ribosomal DNA (rDNA) ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.697-703.1991
更新日期:1991-01-01 00:00:00
abstract::The highly conserved DnaK chaperones consist of an N-terminal ATPase domain, a central substrate-binding domain, and a C-terminal domain whose function is not known. Since Bacillus subtilis dnaK was not able to complement an Escherichia coli dnaK null mutant, we performed domain element swap experiments to identify th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.6.1971-1974.1999
更新日期:1999-03-01 00:00:00
abstract::Production of bacterial flagella is controlled by a multitiered regulatory system that coordinates the expression of 40 to 50 subunits and ordered assembly of these elaborate structures. Flagellar expression is environmentally controlled, presumably to optimize the benefits and liabilities of having these organelles o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00576-17
更新日期:2018-03-12 00:00:00
abstract::We have sequenced the entire region of DNA required for the biosynthesis of CS5 pili from enterotoxigenic Escherichia coli O115:H40 downstream of the major subunit gene, designated csfA (for coli surface factor five A). Five more open reading frames (ORFs) (csfB, csfC, csfE, csfF, and csfD) which are transcribed in th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.18.5847-5851.1999
更新日期:1999-09-01 00:00:00
abstract::Cells of Clostridium thermosaccharolyticum grown under strict anaerobiosis (modified Hungate technique) were examined during growth and sporulation by employing Nomarski interference-contrast and Zernike phase-contrast optics to delineate the sequence of morphological changes leading to the formation of free, mature s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.3.674-681.1972
更新日期:1972-09-01 00:00:00
abstract::Gilden, R. V. (Wistar Institute, Philadelphia, Pa.), and R. I. Carp. Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells. J. Bacteriol. 91:1295-1297. 1966.-Synthesis of the simian virus 40 (SV40) T antigen in primary African green monkey kidney cells was abolis...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.3.1295-1297.1966
更新日期:1966-03-01 00:00:00
abstract::Hyatt, Mildred T. (Pioneering Research Division. U.S. Army Natick Laboratories, Natick, Mass.), and Hillel S. Levinson. Effect of sugars and other carbon compounds on germination and postgerminative development of Bacillus megaterium spores. J. Bacteriol. 88:1403-1415. 1964.-A total of 77 carbon-containing compounds w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.5.1403-1415.1964
更新日期:1964-11-01 00:00:00
abstract::The synthesis of pyruvate carboxylase (PC) was studied by using quantitative immunoblot analysis with an antibody raised against PC purified from Rhodobacter capsulatus and was found to vary 20-fold depending on the growth conditions. The PC content was high in cells grown on pyruvate or on carbon substrates metaboliz...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.5.1460-1468.1997
更新日期:1997-03-01 00:00:00
abstract::Aeromonas hydrophila AH-3 lateral flagella are not assembled when bacteria grow in liquid media; however, lateral flagellar genes are transcribed. Our results indicate that A. hydrophila lateral flagellar genes are transcribed at three levels (class I to III genes) and share some similarities with, but have many impor...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01994-12
更新日期:2013-04-01 00:00:00
abstract::Penicillin-binding protein 4a (PBP4a) from Bacillus subtilis was overproduced and purified to homogeneity. It clearly exhibits DD-carboxypeptidase and thiolesterase activities in vitro. Although highly isologous to the Actinomadura sp. strain R39 DD-peptidase (B. Granier, C. Duez, S. Lepage, S. Englebert, J. Dusart, O...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.5.1595-1599.2001
更新日期:2001-03-01 00:00:00
abstract::Smith, P. B. (Communicable Disease Center, Atlanta, Ga.). Growth of staphylococci on mercuric chloride agar. J. Bacteriol. 84:1016-1019. 1962.-An attempt has been made to corroborate a report that "epidemic" strains of staphylococci are more resistant to mercuric salts than are "nonepidemic" strains. A comparison of m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.5.1016-1019.1962
更新日期:1962-11-01 00:00:00
abstract::Bacteriocin 28b from Serratia marcescens binds to Escherichia coli outer membrane proteins OmpA and OmpF and to lipopolysaccharide (LPS) core (J. Enfedaque, S. Ferrer, J. F. Guasch, J. Tomás, and M. Requé, Can. J. Microbiol. 42:19-26, 1996). A cosmid-based genomic library of S. marcescens was introduced into E. coli N...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.19.5741-5747.1996
更新日期:1996-10-01 00:00:00
abstract::Rhodobacter sphaeroides maintained intracellular ammonium pools of 1.1 to 2.6 mM during growth in several fixed nitrogen sources as well as during diazotrophic growth. Addition of 0.15 mM NH4+ to washed, nitrogen-free cell suspensions was followed by linear uptake of NH4+ from the medium and transient formation of int...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.4.1632-1638.1987
更新日期:1987-04-01 00:00:00
abstract::The occurrence of the novel regulatory nucleotide bis(3',5')-cyclic diguanylic acid (c-di-GMP) and its relation to cellulose biogenesis in the plant pathogen Agrobacterium tumefaciens was studied. c-di-GMP was detected in acid extracts of 32P-labeled cells grown in various media, and an enzyme responsible for its form...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.12.6649-6655.1989
更新日期:1989-12-01 00:00:00
abstract::Filaments of a thermosensitive Bacillus megaterium mutant showed an altered macromolecular composition compared with salt-cured mutant cells and parental cells. Filaments contained more peptidoglycan, polyglucose, poly-beta-hydroxy-butyrate, and deoxyribonucleic acid per unit of protein. The ribonucleic acid-to-protei...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.1.454-456.1980
更新日期:1980-10-01 00:00:00